Разработка и применение современных лабораторных методов в эпидемиологическом мониторинге, диагностики и лечении энтеровирусных инфекций

У роботі проведена порівняльна оцінка специфічності й чутливості тест-системи ПЛР, зі специфічним праймером до ДНК 207 п.н. 5'-нетрансльованої області генома энтеровируса для всіх типів ентеровірусів (крім вірусу поліомієліту) у порівнянні із класичним культуральним методом. А також методологічний підхід спільного використання вищеописаної реакції ПЦР із визначенням антитіл класу Іg до вірусів Коксаки й ЕСНО у системі ІФА діагностики, розробленої авторами, і спектр застосування розробленого комплекс.In work the estimation of specificity and sensitivity of test system PTSR, with specific primers to DNA 207 n.n is spent comparative. 5 '-not broadcast areas генома an enterovirus for all types of enteroviruses (except a poliomyelitis virus) in comparison with classical the virology a method. And also the methodological approach of sharing of above described reaction PCR with definition of antibodies of a class ІgG to viruses Cocsaki and ЕСНО in system IFA of diagnostics developed by authors, and a spectrum of application of the developed complex

Significant impact of different oxygen breathing conditions on noninvasive in vivo tumor-hypoxia imaging using [18F]-fluoro-azomycinarabino-furanoside ([18F]FAZA)

<p>Abstract</p> <p>Background</p> <p>[¹⁸F]FAZA is a PET biomarker with great potential for imaging tumor hypoxia. Aim of our study was to compare [¹⁸F]FAZA uptake in mice with subcutaneous exogenous CT26 colon carcinomas and endogenous polyoma middle-T (PyV-mT) mammary carcinomas and to analyze the influence of different breathing protocols in CT26 colon carcinomas as well as the reversibility or irreversibility of [¹⁸F]FAZA uptake.</p> <p>Methods</p> <p>We injected subcutaneous CT26 colon carcinoma or polyomavirus middle-T (PyV-mT) mammary carcinoma-bearing mice intravenously with¹⁸F-FAZA and performed PET scans 1-3 h post injection (<it>p.i.</it>). To analyze the impact of oxygen supply in CT26 carcinomas we used three different breathing protocols: (P0) air; (P1) 100% oxygen 1 h prior injection until 3 h <it>p.i.</it>; (P2) 100% oxygen breathing starting 2 min prior tracer injection until 1 h <it>p.i. </it>and during the PET scans; mice were breathing air between the 2 h and 3 h 10 min static scans. Normalized PET images were analyzed by using defined regions of interest. Finally, some mice were dissected for pimonidazole immunohistochemistry.</p> <p>Results</p> <p>There was no difference in¹⁸F-FAZA uptake 1-3 h <it>p.i. </it>between the two carcinoma types (CT26: 1.58 ± 0.45%ID/cc; PyV-mT: 1.47 ± 0.89%ID/cc, 1 h <it>p.i.</it>, tumor size < 0.5 cm³). We measured a significant tracer clearance, which was more pronounced in muscle tissue (P0). The [¹⁸F]FAZA tumor-to-muscle-ratios in CT26 colon carcinoma-bearing mice 2 h and 3 h, but not 1 h <it>p.i. </it>were significantly higher when the mice breathed air (P0: 3.56 ± 0.55, 3 h) compared to the oxygen breathing protocols (P1: 2.45 ± 0.58; P2: 2.77 ± 0.42, 3 h). Surprisingly, the breathing protocols P1 and P2 showed no significant differences in T/M ratios, thus indicating that the crucial [¹⁸F]FAZA uptake phase is during the first hour after [¹⁸F]FAZA injection. Importantly, the muscle clearance was not affected by the different oxygen breathing conditions while the tumor clearance was lower when mice were breathing air.</p> <p>Conclusion</p> <p>Exogenous CT26 colon carcinomas and endogenous polyoma middle-T (PyV-mT) mammary carcinomas showed no differences in [¹⁸F]FAZA uptake 1-3 h <it>p.i. </it>Our analysis using various breathing protocols with air (P0) and with pure oxygen (P1, P2) clearly indicate that [¹⁸F]FAZA is an appropriate PET biomarker for <it>in vivo </it>analysis of hypoxia revealing an enhanced tracer uptake in tumors with reduced oxygen supply. [¹⁸F]FAZA uptake was independent of tumor-type.</p

LS-DYNA CONTACT PROCEDURE ANALYSIS FOR SELECTED MECHANICAL SYSTEMS

Finite Element Method is one of the most frequently used computational schemes in numerical analyses. A contact phenomenon is an essential issue when modelling the physical interaction between two or more bodies. Depending on the used software, the various contact algorithms are applied. In the paper, the authors present the results of evaluation of different contact methods implemented in LS-Dyna CAE software. The most common problem arising during contact modelling is the occurrence of penetration between the coupling elements, which leads to an increase of contact forces and, consequently, a local deformation of finite elements. In the introduction, a theoretical background related to mathematical aspects of contact modelling is presented, in particular search methods for contact surfaces and the penalty forces calculating method. Subsequently, a contact analysis for different variants of interaction of the elements is presented. In the first analysed case, shaft- sleeve interaction, in both rotation and translation, is presented. The variable factors in this case were a number of interacting finite elements and the type of a contact algorithm. The second case focused on the interaction forces resulting from the interaction between two sliding bodies in the harmonic motion. A sliding body was pressed against the plane using an increasing force. A variable factor in this case was the type of the implemented contact algorithm

CHEMISTRY IN SUPERACIDS .8. SUPERACID-CATALYZED CARBONYLATION OF METHANE, METHYL HALIDES, METHYL-ALCOHOL, AND DIMETHYL ETHER TO METHYL ACETATE AND ACETIC-ACID

Superacid-catalyzed (BF, BF3-H2O, HF-BF3, and CF3SO3H) carbonylation of methane and its substituted derivatives (particularly, methyl alcohol and dimethyl ether) gave acetic acid and methyl acetate. HF-BF3 was found to be the most effective catalyst, giving nearly complete conversion of methyl alcohol or dimethyl ether. CF3SO3H led to lower yields and also to the formation of methyl trifluoromethanesulfonate. Possible reaction pathways and mechanisms are discussed on the basis of experimental results. Contrasted with Rh-catalyzed carbonylation of methyl alcohol, the superacid-catalyzed reaction does not necessitate expensive catalyst and conditions necessary with sensitive organometallic catalyst systems

Interaction of Linear Polyelectrolytes with Proteins: Role of Specific Charge-Charge Interaction and Ionic Strength

We present a thermodynamic study of the interaction of synthetic, linear polyelectrolytes with bovine serum albumin (BSA). All polyelectrolytes are based on poly(allyl glycidyl ether) which has been modified by polymer-analogous reaction with anionic (-SO3Na), cationic (-NH3Cl or -NHMe2Cl) or zwitterionic groups (-NMe2(CH2)3SO3). While the anionic polymer shows a very weak interaction, the zwitterionic polymer exhibits no interaction with BSA (pI = 4.7) under the applied pH = 7.4, ionic strength (I = 23–80 mM) and temperature conditions (T = 20–37 °C). A strong binding, however, was observed for the polycations bearing primary amino or tertiary dimethyl amino groups, which could be analysed in detail by isothermal titration calorimetry (ITC). The analysis was done using an expression which describes the free energy of binding, ΔGb, as the function of the two decisive variables, temperature, T, and salt concentration, cs. The underlying model splits ΔGb into a term related to counterion release and a term related to water release. While the number of released counter ions is similar for both systems, the release of bound water is more important for the primary amine compared to the tertiary N,N-dimethyl amine presenting polymer. This finding is further traced back to a closer contact of the polymers’ protonated primary amino groups in the complex with oppositely charged moieties of BSA as compared to the bulkier protonated tertiary amine groups. We thus present an investigation that quantifies both driving forces for electrostatic binding, namely counterion release and change of hydration, which contribute to a deeper understanding with direct impact on future advancements in the biomedical field

LS-Dyna contact procedure analysis for selected mechanical systems

Finite Element Method is one of the most frequently used computational schemes in numerical analyses. A contact phenomenon is an essential issue when modelling the physical interaction between two or more bodies. Depending on the used software, the various contact algorithms are applied. In the paper, the authors present the results of evaluation of different contact methods implemented in LS-Dyna CAE software. The most common problem arising during contact modelling is the occurrence of penetration between the coupling elements, which leads to an increase of contact forces and, consequently, a local deformation of finite elements. In the introduction, a theoretical background related to mathematical aspects of contact modelling is presented, in particular search methods for contact surfaces and the penalty forces calculating method. Subsequently, a contact analysis for different variants of interaction of the elements is presented. In the first analysed case, shaft- sleeve interaction, in both rotation and translation, is presented. The variable factors in this case were a number of interacting finite elements and the type of a contact algorithm. The second case focused on the interaction forces resulting from the interaction between two sliding bodies in the harmonic motion. A sliding body was pressed against the plane using an increasing force. A variable factor in this case was the type of the implemented contact algorithm

Hippocampal activity predicts contextual misattribution of false memories

Failure of contextual retrieval can lead to false recall, wherein people retrieve an item or experience that occurred in a different context or did not occur at all. Whereas the hippocampus is thought to play a crucial role in memory retrieval, we lack understanding of how the hippocampus supports retrieval of items related to a target context while disregarding related but irrelevant information. Using direct electrical recordings from the human hippocampus, we investigate the neural process underlying contextual misattribution of false memories. In two large datasets, we characterize key physiological differences between correct and false recalls that emerge immediately prior to vocalization. By differentiating between false recalls that share high or low contextual similarity with the target context, we show that low-frequency activity (6 to 18 Hz) in the hippocampus tracks similarity between the current and retrieved context. Applying multivariate decoding methods, we were able to reliably predict the contextual source of the to-be-recalled item. Our findings elucidate one of the hallmark features of episodic memory: our ability to distinguish between memories that were formed on different occasions

Exhumation of the High‐Pressure Tsäkkok Lens, Swedish Caledonides: Insights From the Structural and White Mica 40Ar/39Ar Geochronological Record

Integrated structural, geochemical, and geochronological investigations were conducted on metasedimentary rocks in the eclogite-bearing Tsäkkok Lens of the Seve Nappe Complex (Scandinavian Caledonides) to resolve its exhumation history. Three deformation events are defined. D1 is likely related to the prograde to peak-metamorphic stages, represented by a locally preserved S1. D2 resulted in vertical shortening and is defined by a pervasive S2 and cm-/m-scale F2 closed folds. D2 terminated with Scandian thrusting, which emplaced the overlying Köli Nappe Complex. D3 records NE-SW shortening and constitutes m-/km-scale F3 open folds that deformed the Tsäkkok Lens and Köli Nappe Complex together. In situ white mica 40Ar/39Ar geochronology was conducted on select metasedimentary samples possessing S1 or S2 to resolve the timing of exhumation. Postdecompression cooling of the Tsäkkok Lens is best recorded by samples containing S1 or S2 that yield homogeneous white mica chemistry and 40Ar/39Ar dates. The timing of cooling is resolved to 477.2 ± 4.1 Ma (S1) and 475.3 ± 3.5 Ma (S2). Vertical shortening of the lens during exhumation may have proceeded until 458.1 ± 9.0 Ma. Later-stage deformation during Scandian thrusting penetrated the Tsäkkok Lens at 429.9 ± 9.0 Ma, or younger. This resulted in noncoaxial deformation of the metasedimentary rocks, producing heterogeneous white mica chemistry and partially reset the older 40Ar/39Ar cooling record. Temperatures for deformation are resolved to the upper greenschist‐lower amphibolite facies. Altogether, the Tsäkkok Lens records rapid exhumation from eclogite‐facies conditions to midcrustal depths or shallower, followed by emplacement of the overlying Köli Nappe Complex