75 research outputs found
GPCRs from fusarium graminearum detection, modeling and virtual screening - the search for new routes to control head blight disease.
201
Structure-based virtual screening of hypothetical inhibitors of the enzyme longiborneol synthase: a potential target to reduce Fusarium head blight disease.
Meloidogyne incognita PASSE-MURAILLE (MiPM) gene encodes a cell-penetrating protein that interacts with the CSN5 subunit of the COP9 Signalosome.
Na publicação: Erika V. S. Albuquerque and Maria F. Grossi-de-Sá
Meloidogyne incognita PASSE-MURAILLE (MiPM) Gene Encodes a Cell-Penetrating Protein That Interacts With the CSN5 Subunit of the COP9 Signalosome
The pathogenicity of phytonematodes relies on secreted virulence factors to rewire host cellular pathways for the benefits of the nematode. In the root-knot nematode (RKN) Meloidogyne incognita, thousands of predicted secreted proteins have been identified and are expected to interact with host proteins at different developmental stages of the parasite. Identifying the host targets will provide compelling evidence about the biological significance and molecular function of the predicted proteins. Here, we have focused on the hub protein CSN5, the fifth subunit of the pleiotropic and eukaryotic conserved COP9 signalosome (CSN), which is a regulatory component of the ubiquitin/proteasome system. We used affinity purification-mass spectrometry (AP-MS) to generate the interaction network of CSN5 in M. incognita-infected roots. We identified the complete CSN complex and other known CSN5 interaction partners in addition to unknown plant and M. incognita proteins. Among these, we described M. incognita PASSE-MURAILLE (MiPM), a small pioneer protein predicted to contain a secretory peptide that is up-regulated mostly in the J2 parasitic stage. We confirmed the CSN5-MiPM interaction, which occurs in the nucleus, by bimolecular fluorescence complementation (BiFC). Using MiPM as bait, a GST pull-down assay coupled with MS revealed some common protein partners between CSN5 and MiPM. We further showed by in silico and microscopic analyses that the recombinant purified MiPM protein enters the cells of Arabidopsis root tips in a non-infectious context. In further detail, the supercharged N-terminal tail of MiPM (NTT-MiPM) triggers an unknown host endocytosis pathway to penetrate the cell. The functional meaning of the CSN5-MiPM interaction in the M. incognita parasitism is discussed. Moreover, we propose that the cell-penetrating properties of some M. incognita secreted proteins might be a non-negligible mechanism for cell uptake, especially during the steps preceding the sedentary parasitic phase
A chemosensory GPCR as a potential target to control the Root-Knot Nematode Meloidogyne incognita parasitism in plants.
Made available in DSpace on 2019-10-24T18:15:24Z (GMT). No. of bitstreams: 1
molecules2403798.pdf: 19607038 bytes, checksum: 72043c875bb4164ff917bd5eeeed827d (MD5)
Previous issue date: 2019bitstream/item/203619/1/molecules-24-03798.pd
Systematic analysis, comparison, and integration of disease based human genetic association data and mouse genetic phenotypic information
Multiple Polymorphisms Affect Expression and Function of the Neuropeptide S Receptor (NPSR1)
Peer reviewe
Germline Genetic Contributions to Risk for Esophageal Adenocarcinoma, Barrett's Esophagus, and Gastroesophageal Reflux
Esophageal adenocarcinoma (EA) is an increasingly common cancer with poor survival. Barrett’s esophagus (BE) is the main precursor to EA, and every year 0.12% to 0.5% of BE patients progress to EA. BE typically arises on a background of chronic gastroesophageal reflux (GERD), one of the risk factors for EA
Prevalence of Helicobacter pylori infection in patients with inflammatory bowel disease: pilot study Prevalencia de la infecciĂłn por Helicobacter pylori en pacientes con enfermedad inflamatoria intestinal: un estudio piloto
- …