Agromorphological genetic diversity of Spanish traditional melons

[EN] The variability of 62 Spanish landraces (and two hybrids used as reference) was described by analysing 39 morphological traits and eight SSR makers. Results showed that 81% of the examined genetic pool belonged to the inodorus type. Spanish traditional melons presented fruits from flattened and globular shapes to elliptical. Rind colour varied from pale green, almost white and yellow to dark green and almost black. Rind texture varied from smooth to intensely wrinkled. Spanish landraces also had larger fruits (average fruit weight 2.6 kg) and longer vegetative cycles (117 days to maturity) compared to landraces from other geographical origins. Farmers seem to have focused on selection towards large fruits, which usually requires the longest production cycles. Fruit colour, size and shape seemed to have been determinant in varietal selection. Hierarchical clustering resulted in two main groups (climacteric and non-climacteric). The largest group was composed of 60 accessions of non-climacteric types, which includes the most demanded by national markets, 'Piel de Sapo', which fruits were characterized by an ovate or elliptical shape, a green rind, big spots and stains distributed over the whole fruit, a rounded blossom end shape and a very pointed stem end shape. The study demonstrates that the Spanish genetic pool is much more diverse. Wide variability was found in a geographical area with vast historical importance in melon farming. These evaluations has allowed the identification of several uniform groups of non-climacteric cultivars ('Piel de Sapo', 'Mochuelo', 'Tendral', Yellow/White, Winter and Black groups) and a set of highly variable climacteric ones. However, many accessions with singular properties remain unclassified, demonstrating the morphological variability of the studied collection. Melons in Spain have wide variability together with a vast historical importance on farms. Some fruit types, or at least some morphological characters reflected by painters during centuries, have reached the present. The present study confirmed the need to preserve these irreplaceable genetic resources and continue their study and evaluation for valuable traits which could enhance farmer's opportunities for entering new markets.The authors would like to thank Maria Jose Diez, the curator of the COMAV's Genebank for providing some of the melon varieties included in this study. Financial support was received from the Genome-Spain Foundation through the Project MELONOMICS (http://www.gen-es.org/es/melanomics.cfm). This study was partially supported by the Project AGL2014-53398-C2-R (funded by the Spanish Economy and Competitiveness Department, and the European Regional Development Fund).Lázaro, A.; Fernandez, I.; Borrero, M.; Cabello, F.; Lopez-Sese, A.; Gómez-Guillamon, M.; Picó Sirvent, MB. (2017). Agromorphological genetic diversity of Spanish traditional melons. Genetic Resources and Crop Evolution. 64(7):1687-1706. doi:10.1007/s10722-016-0466-0S16871706647Alvarez de Sotomayor JM (1824) Columela (AD 4–AD 70) Los Doce Libros de Agricultura. Traducidos al castellano por D.Juan María Alvarez de Sotomayor y Rubio, SantanderBlanca J, Esteras C, Ziarsolo P, Perez D, Fernandez-Pedrosa V, Collado C, Rodriguez de Pablos R, Ballester A, Roig C, Canizares J, Pico B (2012) Transcriptome sequencing for SNP discovery across Cucumis melo. BMC Genomics 13:280. doi: 10.1186/1471-2164-13-280Camacho-Villa TC, Maxted N, Scholtena M, Ford-Lloyd B (2005) Defining and identifying crop landraces. Plant Genet Resour Charact Util 3(3):373–384Cubero JI (2001) El libro de agricultura de Al Awam/Edición y comentario sobre la traducción de Banqueri. Empresa Pública para el Desarrollo Agrario y Pesquero de Andalucía, SevillaECPGR Working Group on Cucurbits (2008) Minimum descriptors for Cucurbita spp., cucumber, melon and watermelon. ECPGR Secretariat, Rome, ItalyEscribano S, Lázaro A (2009) Agro-morphological diversity of Spanish traditional melons (Cucumis melo L.) of the Madrid provenance. Genet Resour Crop Evol 56(4):481–497Escribano S, Lázaro A (2012) Sensorial characteristics of Spanish traditional melon genotypes: has the flavor of melon changed in the last century? Eur Food Res Technol 234(4):581–592. doi: 10.1007/s00217-012-1661-7Escribano S, Lázaro A, Cuevas HE, Lopez-Sese AI, Staub JE (2012) Spanish melons (Cucumis melo L.) of the Madrid provenance: a unique germplasm reservoir. Genet Resour Crop Evol 59(3):359–373Esquinas-Alcázar JT, Gulik PJ (1983) Genetic resources of Cucurbitaceae: a global report. International Board of Plant Genetic Resources, RomeEsteras C, Nuez F, Picó MB (2011) Genetic diversity studies in cucurbits using molecular tools. In: Wang Y-H, Behera TK, Kole C (eds) Genetics, genomics and breeding of crop plants. Genetics, genomics and breeding of cucurbits. Science, pp 225–255Esteras C, Formisano G, Roig C, Diaz A, Blanca J, Garcia-Mas J, Gomez-Guillamon ML, Lopez-Sese AI, Lazaro A, Monforte AJ, Pico B (2013) SNP genotyping in melons: genetic variation, population structure, and linkage disequilibrium. Theor Appl Genet 126(5):1285–1303FAO (2013) FAOSTAT-trade and markets. http://www.fao.org/es/esc/en/index.html . Accessed 31 Mar 2016Fernandez-Trujillo JP, Fernandez-Talavera M, Ruiz-Leon M, Roca MJ, Dos-Santos N (2012) Aroma volatiles during whole melon ripening in a climacteric near-isogenic line and its inbred non-climacteric parents. International Society of Horticulture Science, Leuven, p 1Gao P, Ma H, Luan F, Song H (2012) DNA fingerprinting of Chinese melon provides evidentiary support of seed quality appraisal. PLoS ONE 7(12): e52431. doi: 10.1371/journal.pone.0052431Hammer K, Gladis T (2014) Notes on infraspecific nomenclature and classifications of cultivated plants in Compositae, Cruciferae, Cucurbitaceae, Gramineae (with a remark on Triticum dicoccon Schrank) and Leguminosae. Genet Resour Crop Evol 61(8):1455–1467Herrera GA (1777) Agricultura general. Don Antonio de Sancha, MadridKerje T, Grum M (2000) The origin of melon, Cucumis melo: a review of the literature. Acta Hortic 510:37–44Kimura M, Crow JF (1964) The number of alleles that can be maintained in a finite population. Genetics 49:725–738Laguna A (1991) Pedacio Dioscorides anazarbeo, Acerca de la materia medicinal y de los venenos mortiferos. Consejería de Agricultura y Cooperación de la Comunidad, Secretaría General Técnica, MadridLázaro A, Fernández-Navarro I, Cabello F, de Lorenzo C (2010) Catálogo de variedadres de melón tradicionales españolas. IMIDRA, Madrid, ISBN-10: 84-695-6262-2. www.madrid.org/bvirtual/BVCM003438.pdfLázaro A, Fernández-Navarro I, de Lorenzo C (2016) Using landraces in agriculture, food and cooking: experiences around a big city in Southern Europe. In: Maxted N, Dulloo MF-LB (eds) Enhancing crop genepool use. CABI, Birmingham, pp 313–317Levene H (1949) On a matching problem arising in genetics. Ann Math Stat 20:91–94Lewontin RC (1972) The apportionment of human diversity. Evol Biol 6:381–398López-Sesé AI, Staub JE, Gómez-Guillamón ML (2003) Genetic analysis of Spanish melon (Cucumis melo L.) germplasm using a standardized molecular-marker array and geographically diverse reference accessions. Theor Appl Genet 108(1):41–52Lotti C, Marcotrigiano A, De Giovanni C, Resta P, Ricciardi A, Zonno V, Fanizza G, Ricciardi L (2008) Univariate and multivariate analysis performed on bio-agronomical traits of Cucumis melo L. germplasm. Genet Resour Crop Evol 55(4):511–522McCreight JD, Nerson H, Grumet R (1993) Melon, Cucumis melo L. In: Kalloo G, Berkg BO (eds) Genetic improvement of vegetable crops. Pergamon Press, New York, pp 267–294Negri V, Becker H, Onnela J, Sartori A, Strajeru S, Laliberté B (2000) A first inventory of on-farm conservation and management activities in Europe including examples of formal and informal sector cooperation. In: Laliberté B, Maggioni L, Maxted N, Negri V (eds) ECP/GR In situ and on-farm conservation network. Report of a task force on wild species conservation in genetic reserves and a task force on on-farm conservation and management. Isola Polvese, Italy, pp 15–31Nei M (1973) Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci USA 70(12):3321–3323Paris HS, Amar Z, Lev E (2012) Medieval emergence of sweet melons, Cucumis melo (Cucurbitaceae). Ann Bot 110(1):23–33Pitrat M (2008) Melon (Cucumis melo L.). In: Prohens J, Nuez F (eds) Handbook of crop breeding: vegetables, vol 1. Springer, New York, pp 283–315Rodriguez-Mohedano R, Rodriguez-Mohedano P (1781) Historia Literaria de España. Joachin Ibarra, MadridRoy A, Bal SS, Fergany M, Kaur S, Singh H, Malik A, Singh J, Monforte AJ, Dhillon NPS (2012) Wild melon diversity in India (Punjab State). Genet Resour Crop Evol 59(5):755–767Sensoy S, Buyukalaca S, Abak K (2007) Evaluation of genetic diversity in Turkish melons (Cucumis melo L.) based on phenotypic characters and RAPD markers. Genet Resour Crop Evol 54(6):1351–1365Spataro G, Negri V (2013) The European seed legislation on conservation varieties: focus, implementation, present and future impact on landrace on farm conservation. Genet Resour Crop Evol 60(8):2421–2430Staub JE, Danin-Poleg Y, Fazio G, Horejsi T, Reis N, Katzir N (2000) Comparative analysis of cultivated melon groups (Cucumis melo L.) using random amplified polymorphic DNA and simple sequence repeat markers. Euphytica 115(3):225–241Staub J, López-Sesé A, Fanourakis N (2004) Diversity among melon landraces (Cucumis melo L.) from Greece and their genetic relationships with other melon germplasm of diverse origins. Euphytica 136(2):151–166Szamosi C, Solmaz I, Sari N, Barsony C (2010) Morphological evaluation and comparison of Hungarian and Turkish melon (Cucumis melo L.) germplasm. Sci Hortic 124(2):170–182Tomason Y, Nimmakayala P, Levi A, Reddy U (2013) Map-based molecular diversity, linkage disequilibrium and association mapping of fruit traits in melon. Mol Breed 31(4):829–841Tzitzikas EN, Monforte AJ, Fatihi A, Kypriotakis Z, Iacovides TA, Ioannides IM, Kalaitzis P (2009) Genetic diversity and population structure of traditional Greek and Cypriot melon cultigens (Cucumis melo L.) based on simple sequence repeat variability. HortScience 44(7):1820–182

Theoretical Models of Sunspot Structure and Dynamics

Recent progress in theoretical modeling of a sunspot is reviewed. The observed properties of umbral dots are well reproduced by realistic simulations of magnetoconvection in a vertical, monolithic magnetic field. To understand the penumbra, it is useful to distinguish between the inner penumbra, dominated by bright filaments containing slender dark cores, and the outer penumbra, made up of dark and bright filaments of comparable width with corresponding magnetic fields differing in inclination by some 30 degrees and strong Evershed flows in the dark filaments along nearly horizontal or downward-plunging magnetic fields. The role of magnetic flux pumping in submerging magnetic flux in the outer penumbra is examined through numerical experiments, and different geometric models of the penumbral magnetic field are discussed in the light of high-resolution observations. Recent, realistic numerical MHD simulations of an entire sunspot have succeeded in reproducing the salient features of the convective pattern in the umbra and the inner penumbra. The siphon-flow mechanism still provides the best explanation of the Evershed flow, particularly in the outer penumbra where it often consists of cool, supersonic downflows.Comment: To appear in "Magnetic Coupling between the Interior and the Atmosphere of the Sun", eds. S.S. Hasan and R.J. Rutten, Astrophysics and Space Science Proceedings, Springer-Verlag, Heidelberg, Berlin, 200

The Evershed Flow and the Brightness of the Penumbra

The Evershed flow is a systematic motion of gas that occurs in the penumbra of all sunspots. Discovered in 1909, it still lacks a satisfactory explanation. We know that the flow is magnetized, often supersonic, and that it shows conspicuous fine structure on spatial scales of 0.2"-0.3", but its origin remains unclear. The hope is that a good observational understanding of the relation between the flow and the penumbral magnetic field will help us determine its nature. Here I review advances in the characterization of the Evershed flow and sunspot magnetic fields from high-resolution spectroscopic and spectropolarimetric measurements. Using this information as input for 2D heat transfer simulations, it has been demonstrated that hot Evershed upflows along nearly horizontal field lines are capable of explaining one of the most intriguing aspects of sunspots: the surplus brightness of the penumbra relative to the umbra. They also explain the existence of penumbral filaments with dark cores. These results support the idea that the Evershed flow is largely responsible for the transport of energy in the penumbra.Comment: 18 pages, to appear in "Magnetic Coupling between the Interior and the Atmosphere of the Sun", eds. S.S. Hasan and R.J. Rutten, Astrophysics and Space Science Proceedings, Springer, Heidelberg, 200

Convection and the Origin of Evershed Flows

Numerical simulations have by now revealed that the fine scale structure of the penumbra in general and the Evershed effect in particular is due to overturning convection, mainly confined to gaps with strongly reduced magnetic field strength. The Evershed flow is the radial component of the overturning convective flow visible at the surface. It is directed outwards -- away from the umbra -- because of the broken symmetry due to the inclined magnetic field. The dark penumbral filament cores visible at high resolution are caused by the 'cusps' in the magnetic field that form above the gaps. Still remaining to be established are the details of what determines the average luminosity of penumbrae, the widths, lengths, and filling factors of penumbral filaments, and the amplitudes and filling factors of the Evershed flow. These are likely to depend at least partially also on numerical aspects such as limited resolution and model size, but mainly on physical properties that have not yet been adequately determined or calibrated, such as the plasma beta profile inside sunspots at depth and its horizontal profile, the entropy of ascending flows in the penumbra, etc.Comment: 13 pages, 7 figures. To appear in "Magnetic Coupling between the Interior and the Atmosphere of the Sun", eds. S.S. Hasan and R.J. Rutten, Astrophysics and Space Science Proceedings, Springer-Verlag, Heidelberg, Berlin, 200

Time-to-pregnancy and pregnancy outcomes in a South African population

<p>Abstract</p> <p>Background</p> <p>Time-to-pregnancy (TTP) has never been studied in an African setting and there are no data on the rates of adverse pregnancy outcomes in South Africa. The study objectives were to measure TTP and the rates of adverse pregnancy outcomes in South Africa, and to determine the reliability of the questionnaire tool.</p> <p>Methods</p> <p>The study was cross-sectional and applied systematic stratified sampling to obtain a representative sample of reproductive age women for a South African population. Data on socio-demographic, work, health and reproductive variables were collected on 1121 women using a standardized questionnaire. A small number (n = 73) of randomly selected questionnaires was repeated to determine reliability of the questionnaire. Data was described using simple summary statistics while Kappa and intra-class correlation statistics were calculated for reliability.</p> <p>Results</p> <p>Of the 1121 women, 47 (4.2%) had never been pregnant. Mean gravidity was 2.3 while mean parity was 2.0 There were a total of 2467 pregnancies; most (87%) resulted in live births, 9.5% in spontaneous abortion and 2.2% in still births. The proportion of planned pregnancies was 39% and the median TTP was 6 months. The reliability of the questionnaire for TTP data was good; 63% for all participants and 97% when censored at 14 months. Overall reliability of reporting adverse pregnancy outcomes was very high, ranging from 90 - 98% for most outcomes.</p> <p>Conclusion</p> <p>This is the first comprehensive population-based reproductive health study in South Africa, to describe the biologic fertility of the population, and provides rates for planned pregnancies and adverse pregnancy outcomes. The reliability of the study questionnaire was substantial, with most outcomes within 70 - 100% reliability index. The study provides important public information for health practitioners and researchers in reproductive health. It also highlights the need for public health intervention programmes and epidemiological research on biologic fertility and adverse pregnancy outcomes in the population.</p

Effects of fishery protection on biometry and genetic structure of two target sea cucumber species from the Mediterranean Sea

Sea cucumber fisheries are now occurring in most of the tropical areas of the world, having expanded from its origin in the central Indo-Pacific. Due to the overexploitation of these resources and the increasing demand from Asian countries, new target species from Mediterranean Sea and northeastern Atlantic Ocean are being caught. The fishery effects on biometry and genetic structure of two target species (Holothuria polii and H. tubulosa) from Turkey, were assessed. The heaviest and largest individuals of H. polii were found into the non-fishery area of Kusadasi, also showing the highest genetic diversity. Similar pattern was detected in H. tubulosa, but only the weight was significantly higher in the protected area. However, the observed differences on the fishery effects between species, could be explained considering the different percentage of catches (80% for H. polii and 20% for H. tubulosa)

Chemicals released by male sea cucumber mediate aggregation and spawning behaviours

The importance of chemical communication in reproduction has been demonstrated in many marine broadcast spawners. However, little is known about the use of chemical communication by echinoderms, the nature of the compounds involved and their mechanism(s) of action. Here, the hypothesis that the sea cucumber Holothuria arguinensis uses chemical communication for aggregation and spawning was tested. Water conditioned by males, but not females, attracted both males and females; gonad homogenates and coelomic fluid had no effect on attraction. Male spawning water, but not female spawning water, stimulated males and females to release their gametes; the spermatozoa alone did not induce spawning. H. arguinensis male spawning water also induced spawning in the phylogenetically related H. mammata. This indicates that males release pheromones together with their gametes that induce spawning in conspecifics and possibly sympatric species. Finally, the male pheromone seems to be a mixture with at least one labile compound (biological activity is lost after four hours at ambient temperature) possibly including phosphatidylcholines. The identification of pheromones in sea cucumbers offers a new ecological perspective and may have practical applications for their aquaculture.FCT - Foundation for Science and Technology [UID/Multi/04326/2013, SFRH/BD/90761/2012]info:eu-repo/semantics/publishedVersio

Enhanced Botrytis cinerea resistance of Arabidopsis plants grown in compost may be explained by increased expression of defense-related genes, as revealed by microarray analysis

Composts are the products obtained after the aerobic degradation of different types of organic matter waste and can be used as substrates or substrate/soil amendments for plant cultivation. There is a small but increasing number of reports that suggest that foliar diseases may be reduced when using compost, rather than standard substrates, as growing medium. The purpose of this study was to examine the gene expression alteration produced by the compost to gain knowledge of the mechanisms involved in compost-induced systemic resistance. A compost from olive marc and olive tree leaves was able to induce resistance against Botrytis cinerea in Arabidopsis, unlike the standard substrate, perlite. Microarray analyses revealed that 178 genes were differently expressed, with a fold change cut-off of 1, of which 155 were up-regulated and 23 were down-regulated in compost-grown, as against perlite-grown plants. A functional enrichment study of up-regulated genes revealed that 38 Gene Ontology terms were significantly enriched. Response to stress, biotic stimulus, other organism, bacterium, fungus, chemical and abiotic stimulus, SA and ABA stimulus, oxidative stress, water, temperature and cold were significantly enriched, as were immune and defense responses, systemic acquired resistance, secondary metabolic process and oxireductase activity. Interestingly, PR1 expression, which was equally enhanced by growing the plants in compost and by B. cinerea inoculation, was further boosted in compost-grown pathogen-inoculated plants. Compost triggered a plant response that shares similarities with both systemic acquired resistance and ABA-dependent/independent abiotic stress responses