2,383 research outputs found
Childrenâs Digital Play during the COVID-19 Pandemic: insights from the Play Observatory
The COVID-19 pandemic has had an enormous impact on many aspects of childrenâs day-to-day lives, including their play. Measures such as lockdowns, school and playground closures, quarantine, isolation and social distancing introduced to curb transmission have resulted in major consequences for where, when, how and with whom children can play. This article reports on interim findings from âThe Play Observatoryâ, a 15-month project researching childrenâs play experiences during the COVID-19 pandemic. Collecting data through an online survey and online case studies, the research offers insights into ways in which childrenâs play has endured, adapted and responded to restrictions brought about by the pandemic. This article focuses on childrenâs digital play throughout this period, including examples of digital gaming, online play, social media, playful creation of digital media texts and hybrid online-offline play. Drawing on theories relating to dynamic literacies, multimodal perspectives and the Reggio Emilia concept of the âhundred languagesâ, this article examines the role of the digital in childrenâs contemporary play practices and the specific affordances of digital play during times of stress, uncertainty and physical distancing. The findings highlight ways in which digital play continued, adapted, evolved and reflected childrenâs experiences and understandings of the pandemic. The study reveals the complexity of digital play and its place within contemporary digital childhoods, troubling simplistic notions of âscreen timeâ and highlighting the increasingly blurred boundaries around digital and non-digital practices, calling for educational approaches that value digital play as significant meaning-making
Trapped lipopolysaccharide and LptD intermediates reveal lipopolysaccharide translocation steps across the Escherichia coli outer membrane
Lipopolysaccharide (LPS) is a main component of the outer membrane of Gram-negative bacteria, which is essential for the vitality of most Gram-negative bacteria and plays a critical role for drug resistance. LptD/E complex forms a N-terminal LPS transport slide, a hydrophobic intramembrane hole and the hydrophilic channel of the barrel, for LPS transport, lipid A insertion and core oligosaccharide and O-antigen polysaccharide translocation, respectively. However, there is no direct evidence to confirm that LptD/E transports LPS from the periplasm to the external leaflet of the outer membrane. By replacing LptD residues with an unnatural amino acid p-benzoyl-L-phenyalanine (pBPA) and UV-photo-cross-linking in E.coli, the translocon and LPS intermediates were obtained at the N-terminal domain, the intramembrane hole, the lumenal gate, the lumen of LptD channel, and the extracellular loop 1 and 4, providing the first direct evidence and âsnapshotsâ to reveal LPS translocation steps across the outer membrane
Early growth response 2 (EGR2) is a novel regulator of the senescence programme.
Senescence, a state of stable growth arrest, plays an important role in ageing and age-related diseases in vivo. Although the INK4/ARF locus is known to be essential for senescence programmes, the key regulators driving p16 and ARF transcription remain largely underexplored. Using siRNA screening for modulators of the p16/pRB and ARF/p53/p21 pathways in deeply senescent human mammary epithelial cells (DS HMECs) and fibroblasts (DS HMFs), we identified EGR2 as a novel regulator of senescence. EGR2 expression is up-regulated during senescence, and its ablation by siRNA in DS HMECs and HMFs transiently reverses the senescent phenotype. We demonstrate that EGR2 activates the ARF and p16 promoters and directly binds to both the ARF and p16 promoters. Loss of EGR2 down-regulates p16 levels and increases the pool of p16- p21- 'reversed' cells in the population. Moreover, EGR2 overexpression is sufficient to induce senescence. Our data suggest that EGR2 is a direct transcriptional activator of the p16/pRB and ARF/p53/p21 pathways in senescence and a novel marker of senescence
Growth characteristics in individuals with osteogenesis imperfecta in North America: results from a multicenter study.
PurposeOsteogenesis imperfecta (OI) predisposes people to recurrent fractures, bone deformities, and short stature. There is a lack of large-scale systematic studies that have investigated growth parameters in OI.MethodsUsing data from the Linked Clinical Research Centers, we compared height, growth velocity, weight, and body mass index (BMI) in 552 individuals with OI. Height, weight, and BMI were plotted on Centers for Disease Control and Prevention normative curves.ResultsIn children, the median z-scores for height in OI types I, III, and IV were -0.66, -6.91, and -2.79, respectively. Growth velocity was diminished in OI types III and IV. The median z-score for weight in children with OI type III was -4.55. The median z-scores for BMI in children with OI types I, III, and IV were 0.10, 0.91, and 0.67, respectively. Generalized linear model analyses demonstrated that the height z-score was positively correlated with the severity of the OI subtype (Pâ<â0.001), age, bisphosphonate use, and rodding (Pâ<â0.05).ConclusionFrom the largest cohort of individuals with OI, we provide median values for height, weight, and BMI z-scores that can aid the evaluation of overall growth in the clinic setting. This study is an important first step in the generation of OI-specific growth curves
Use of Combined Hartree-Fock-Roothaan Theory in Evaluation of Lowest States of K [Ar]4s^0 3d^1 and Cr+ [Ar]4s^0 3d^5 Isoelectronic Series Over Noninteger n-Slater Type Orbitals
By the use of integer and noninteger n-Slater Type Orbitals in combined
Hartree-Fock-Roothaan method, self consistent field calculations of orbital and
lowest states energies have been performed for the isoelectronic series of open
shell systems K [Ar]4s^0 3d^1 2(D) (Z=19-30) and Cr+ [Ar] 4s^0 3d^5 6(S)
(Z=24-30). The results of calculations for the orbital and total energies
obtained from the use of minimal basis sets of integer- and noninteger n-Slater
Type Orbitals are given in tables. The results are compared with the
extended-basis Hartree-Fock computations. The orbital and total energies are in
good agreement with those presented in the literature. The results are
accurately and considerably can be useful in the application of
non-relativistic and relativistic combined Hartree-Fock-Roothaan approach for
heavy atomic systems.Comment: 11 pages, 6 tables, 2 figures. submitte
Characteristic Evolution and Matching
I review the development of numerical evolution codes for general relativity
based upon the characteristic initial value problem. Progress in characteristic
evolution is traced from the early stage of 1D feasibility studies to 2D
axisymmetric codes that accurately simulate the oscillations and gravitational
collapse of relativistic stars and to current 3D codes that provide pieces of a
binary black hole spacetime. Cauchy codes have now been successful at
simulating all aspects of the binary black hole problem inside an artificially
constructed outer boundary. A prime application of characteristic evolution is
to extend such simulations to null infinity where the waveform from the binary
inspiral and merger can be unambiguously computed. This has now been
accomplished by Cauchy-characteristic extraction, where data for the
characteristic evolution is supplied by Cauchy data on an extraction worldtube
inside the artificial outer boundary. The ultimate application of
characteristic evolution is to eliminate the role of this outer boundary by
constructing a global solution via Cauchy-characteristic matching. Progress in
this direction is discussed.Comment: New version to appear in Living Reviews 2012. arXiv admin note:
updated version of arXiv:gr-qc/050809
Synthesized grain size distribution in the interstellar medium
We examine a synthetic way of constructing the grain size distribution in the
interstellar medium (ISM). First we formulate a synthetic grain size
distribution composed of three grain size distributions processed with the
following mechanisms that govern the grain size distribution in the Milky Way:
(i) grain growth by accretion and coagulation in dense clouds, (ii) supernova
shock destruction by sputtering in diffuse ISM, and (iii) shattering driven by
turbulence in diffuse ISM. Then, we examine if the observational grain size
distribution in the Milky Way (called MRN) is successfully synthesized or not.
We find that the three components actually synthesize the MRN grain size
distribution in the sense that the deficiency of small grains by (i) and (ii)
is compensated by the production of small grains by (iii). The fraction of each
{contribution} to the total grain processing of (i), (ii), and (iii) (i.e., the
relative importance of the three {contributions} to all grain processing
mechanisms) is 30-50%, 20-40%, and 10-40%, respectively. We also show that the
Milky Way extinction curve is reproduced with the synthetic grain size
distributions.Comment: 10 pages, 6 figures, accepted for publication in Earth, Planets, and
Spac
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