Polarization-dependence of anomalous scattering in brominated DNA and RNA molecules, and importance of crystal orientation in single- and multiple-wavelength anomalous diffraction phasing

In this paper the anisotropy of anomalous scattering at the Br K-absorption edge in brominated nucleotides is investigated, and it is shown that this effect can give rise to a marked directional dependence of the anomalous signal strength in X-ray diffraction data. This implies that choosing the correct orientation for crystals of such molecules can be a crucial determinant of success or failure when using single- and multiple-wavelength anomalous diffraction (SAD or MAD) methods to solve their structure. In particular, polarized absorption spectra on an oriented crystal of a brominated DNA molecule were measured, and were used to determine the orientation that yields a maximum anomalous signal in the diffraction data. Out of several SAD data sets, only those collected at or near that optimal orientation allowed interpretable electron density maps to be obtained. The findings of this study have implications for instrumental choices in experimental stations at synchrotron beamlines, as well as for the development of data collection strategy programs

The Lorentzian distance formula in noncommutative geometry

For almost twenty years, a search for a Lorentzian version of the well-known Connes' distance formula has been undertaken. Several authors have contributed to this search, providing important milestones, and the time has now come to put those elements together in order to get a valid and functional formula. This paper presents a historical review of the construction and the proof of a Lorentzian distance formula suitable for noncommutative geometry.Comment: 16 pages, final form, few references adde

Motion Sickness Lessons from the Southern Ocean

BACKGROUND: The objectives were to assess the prevalence, severity, and medication taken, and to look for predictive factors, in order to better identify characteristics of passengers at risk of motion sickness during transport from Hobart in Tasmania to the French polar stations in Antarctica. METHODS: There were 239 passengers who were surveyed over 4 yr with 4 round trips per year using the Motion Sickness Susceptibility Questionnaire (MSSQ), Simulator Sickness Questionnaire (SSQ), state-trait anxiety test (STAI -Trait and STAI -State), and general parameters (age, gender, number of trips, jet-lag, direction of the trip), medication, calculation of the distance of each passenger’s cabin to the Centre of Gravity (CoG.). RESULTS: While the passengers had a low intrinsic sensitivity to motion sickness (MSSQ), 94% reported at least one SSQ symptom of motion sickness, and 38% vomited. Five associated factors were discovered: greater initial sensitivity (MSSQ), anticipation of being ill, younger age, higher level of anxiety at midtrip, and greater distance from the CoG. Of the passengers, there were 54% who took anti-motion sickness medication at different times of the trip, however, these passengers experienced more nausea. This could be due to self-selection since they were more sensitive to motion sickness. CONCLUSION: We identified three predictive factors of motion sickness (greater intrinsic susceptibility, younger age, and greater cabin distance from the CoG). For preventive purposes, two associated factors of MS (anticipation of being ill, MSSQ score) were determined to classify three groups of risk of MS to improve passenger care during the trip

Reporting Charcoal Rot in Chia and Developing a Susceptibility Assay

Chia (Salvia Hispanica) cross breeds were planted in the summer of 2018 with the intent of selective breeding for agricultural benefit. Preexisting pathogens in the soil caused 40-50% fatality of adult plants. This was surprising due to the precursory knowledge that chia has antibiotic and antifungal oils (Elshafie et. al. 2018); chia was only recently documented to be susceptible to Fusarium wilt (Fusarium oxysporum). The primary pathogen responsible was identified as Macrophomina phaseolina (aka charcoal rot); a widespread soilborne pathogen which has multiple commercial hosts (Su et. al. 2001). M. phaesolina on wheat seed vector where used as inoculums (Brandari 2017) for chia to evaluate disease progression and symptoms in chia. Samples of this trial produced M. phaseolina from diseased chia tissues confirming susceptibility to M. phaseolina; in addition carefully sampled root and stem fractions identified the pathology of fungus from root to stem. The two parental varieties of the cross, chia-Pinta and chia-Tropic continue to be compared for their disease resistance to M. phaseolina. Identifying disease resistant genes allows for breeding of resistant cultivars, improving the marketability of chia

Plastid DNA variation in Prunus serotina var. serotina (Rosaceae), a North American tree invading Europe

Black cherry (Prunus serotina) is a tree from North America, where it is often used for economical purposes, whereas it is widespread and invasive in Europe. Plastid DNA variation was first investigated in both its native and invasive ranges using microsatellite loci and sequences of three intergenic spacers (trnT-trnL, trnD-trnT and trnS-trnG). This analysis was focused on P. serotina var. serotina, with the inclusion of samples of closely related taxa. Length variation at a microsatellite locus (ccmp5) and a few sequence polymorphisms were identified among P. serotina samples. Four new primer pairs were then designed to specifically amplify variable regions and a combination of five markers was finally proposed for phylogeographic studies in P. serotina. These loci allow identification of six chlorotypes in P. serotina var. serotina, which may be particularly useful to depict the maternal origins of European invasive population

The Value of a virtual Kitchen to assess the activities of Daily life in Alzheimer Disease

Patients with Alzheimer\u27s disease (AD patients) experience difficulties in everyday life activities that effect independence. These difficulties can be tested using virtual reality (VR) environments that simulate real life situations. The purpose of this study was to examine the value of a virtual kitchen as an assessment tool of everyday life activities in AD patients. We focus on the assessment results obtained in a group of AD patients on a virtual kitchen designed to assess their ability to prepare a virtual cup of coffee using a virtual coffee machine. An identical real daily living task was tested. Twenty-four AD patients were matched to 32 healthy elderly controls. Significant differences in performance between research and control groups were detected on the virtual and the real tasks, with the research group performing worse. In addition, regression analyses revealed that the number of errors in the virtual test was the best predictor for the real assessment and the Instrumental Activities of Daily Living score. This provides initial support for the sensitivity and the ecological validity of the virtual kitchen as an assessment tool of everyday life activities in AD patient

Stress–strain relationships and yielding of metal-organic framework monoliths

Metal-organic frameworks (MOFs) have emerged as a versatile material platform for a wide range of applications. However, the development of practical devices is constrained by their inherently low mechanical stability. The synthesis of MOFs in a monolithic morphology represents a viable way for the transition of these materials from laboratory research to real-world applications. For the design of MOF-based devices, the mechanical characterization of such materials cannot be overlooked. In this regard, stress-strain relationships represent the most valuable tool for assessing the mechanical response of materials. Here, we use flat punch nanoindentation, micropillar compression and Raman microspectroscopy to investigate the stress-strain behaviour of MOF monoliths. A pseudo-plastic flow is observed under indentation, where the confining pressure prevents unstable crack propagation. Material flow is accommodated by grain boundary sliding, with occasional stepwise cracking to accommodate excessive stress building up. Micropillar compression reveals a brittle failure of ZIF-8, while plastic flow is observed for MIL-68

Polychronous Interpretation of Synoptic, a Domain Specific Modeling Language for Embedded Flight-Software

The SPaCIFY project, which aims at bringing advances in MDE to the satellite flight software industry, advocates a top-down approach built on a domain-specific modeling language named Synoptic. In line with previous approaches to real-time modeling such as Statecharts and Simulink, Synoptic features hierarchical decomposition of application and control modules in synchronous block diagrams and state machines. Its semantics is described in the polychronous model of computation, which is that of the synchronous language Signal.Comment: Workshop on Formal Methods for Aerospace (FMA 2009