Rancang Bangun Sistem Informasi Asistensi ORACLE Berbasis Web Di Prodi Sistem Informasi UNIKOM

Learning process in the Computer Laboratory cannot be separated by quantity and computer specs as well as qualified lecturer. learning process in the Oracle Laboratory of Information System Major can not be held optimally, because many students in the laboratory and limited  time for study. Not all students can be accommodate to solve their problems in the laboratory. This problem is solved with the chosen laboratory assistant. During the process of selecting a laboratory assistant do with conventional process. The election process can not produce a qualified assistant. Besides that,  scheduling lab assistants performed manually, resulting in a scheduling conflict with class schedules the assistant and needs to create a schedule and  printing BAP (Berita Acara Perkuliahan) returned.The certificate of assistance is granted at the end of the semester, done manually and can be made repeatedly. Therefore, need to be built Laboratory Information System Assistance Oracle (SIASLORA) that can process data assistant candidate selection, scheduling assistance Oracle lab, printing BAP (Berita Acara Perkuliahan) and printing a  certificate of assistance quickly and accurately. Development method of SIASLORA using prototype development system and structured system approach using context diagram and data flow diagram. The Programing language using PHP (Hypertext Preprocessor) and data base management system (DBMS) using MySQL. With SIASLORA expected to overcome the problems described earlier.Keywords — laboratory Oracle, kcomputerize, information system, SIASLORA

Rancangan Alat Bantu Pembelajaran Daur Hidup Hewan Untuk Siswa SLB Bagian B Tunarungu Berbasis Multimedia

Children with special needs are individuals who are not much different from children in general, but in carrying out educational activities still have obstacles. One of the children with special needs is a deaf child who receives a variety of subject matter, one of which is animal life cycle material in science subjects. In the process of delivering the material is still done conventionally, the teacher conveys through cues so that it becomes an obstacles to deliver material that requires visuals about the processes that occur in the material. The purpose of this study is to create a multimedia application of learning the life cycle of animals to help deaf students in SLBN Cicendo in visualizing the material. The author uses qualitative descriptive research methods, data collection methods using interviews, system development models using prototypes, and tools to design applications using Unified Modeling Language (UML). Based on the design of the application made, the application is built using Adobe Flash CS6 Professional software, so it is expected to help the delivery of material to deaf students more easily and attractively.

The parasporal crystals of Bacillus pumilus strain 15.1: a potential virulence factor?

Bacillus pumilus strain 15.1 was previously found to cause larval mortality in the Med-fly 27 Ceratitis capitata and was shown to produce crystals in association with the spore. As 28 parasporal crystals are well-known as invertebrate-active toxins in entomopathogenic 29 bacteria such as Bacillus thuringiensis (Cry and Cyt toxins) and Lysinibacillus sphaericus (Bin 30 and Cry toxins), the B. pumilus crystals were characterised. The crystals were composed of a 31 45 kDa protein that was identified as an oxalate decarboxylase by peptide mass 32 fingerprinting, N-terminal sequencing and by comparison with the genome sequence of strain 33 15.1. Synthesis of crystals by a plasmid-cured derivative of strain 15.1 (produced using a 34 novel curing strategy), demonstrated that the oxalate decarboxylase was encoded 35 chromosomally. Crystals spontaneously solubilized when kept at low temperatures and the 36 protein produced was resistant to trypsin treatment. The insoluble crystals produced by 37 B. pumilus 15.1 did not show significant toxicity when bioassayed against C. capitata larvae, 38 but once the OxdD protein was solubilized, an increase of toxicity was observed. We also 39 demonstrate that the OxdD present in the crystals has oxalate decarboxylate activity as the 40 formation of formate was detected, which suggests a possible mechanism for B. pumilus 15.1 41 activity. To our knowledge, the characterization of the B. pumilus crystals as oxalate 42 decarboxylase is the first report of the natural production of parasporal inclusions of an 43 enzyme

Using phage display technology to obtain Crybodies active against non-target insects

The insecticidal Cry toxins produced by Bacillus thuringiensis (Bt) are increasingly important in the biological control of insect pests and vectors of human disease. Markets for Bt products and transgenic plants expressing their toxins are driven by their specificity, safety and the move away from chemical control agents. However, the high specificity of Cry toxins can also prove to be a limitation when there is no known Cry toxin active against a particular target. Novel activities can be discovered by screening natural Bt isolates or through modifications of the Cry proteins. Here we demonstrate the use of λ-phage displaying Cry1Aa13 toxin variants modified in domain II loop 2 (Crybodies) to select retargeted toxins. Through biopanning using gut tissue from larvae of the non-target insect Aedes aegypti, we isolated a number of phage for further testing. Two of the overexpressed Cry toxin variants showed significant activity against A. aegypti larvae while another induced mortality at the pupal stage. We present the first report of the use of phage display to identify novel activities toward insects from distant taxonomic Orders and establish this technology based on the use of Crybodies as a powerful tool for developing tailor-made insecticides against new target insects

Concert recording 2016-10-26

[Track 1]. Come raggio di sol / Antonio Caldara -- [Track 2]. Wishing you were somehow here again from The phantom of the opera / Andrew Lloyd Webber -- [Track 3]. Nicht mehr zu dir zu gehen / Johannes Brahms -- [Track 4]. You are not rich from La périchole / Jacques Offenbach -- [Track 5]. Una donna a quindici anni from Cosi fan tutte / Wolfgang Amadeus Mozart -- [Track 6]. Stranger in paradise from Kismet / George Forrest -- [Track 7]. Come paride vezzoso from L\u27elisir d\u27amore / Gaetano Donizetti Questo amor, vergogna mia from Edgar / Giacomo Puccini -- [Track 8]. Dein angesicht / Robert Schumann -- [Track 9]. Mai / Gabriel Faure -- [Track 10]. Ganymed / Franz Schubert -- [Track 11]. The sound of music from The sound of music / Richard Rodgers Oscar Hammerstein II -- [Track 12]. Après un rève / Faure -- [Track 13]. Steal me, sweet thief from The old main and the thief / Fian Carlo Menotti -- [Track 14]. Four encore songs / Florence Price -- [Track 15]. Morire / Puccini -- [Track 16]. Come scoglio from Cosi fan tutte / Mozart

Exploring the boundaries of microbial habitability in soil

© The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Dragone, N. B., Diaz, M. A., Hogg, I., Lyons, W. B., Jackson, W. A., Wall, D. H., Adams, B. J., & Fierer, N. Exploring the boundaries of microbial habitability in soil. Journal of Geophysical Research: Biogeosciences, 126(6), (2021): e2020JG006052, https://doi.org/10.1029/2020JG006052.Microbes are widely assumed to be capable of colonizing even the most challenging terrestrial surface environments on Earth given enough time. We would not expect to find surface soils uninhabited by microbes as soils typically harbor diverse microbial communities and viable microbes have been detected in soils exposed to even the most inhospitable conditions. However, if uninhabited soils do exist, we might expect to find them in Antarctica. We analyzed 204 ice-free soils collected from across a remote valley in the Transantarctic Mountains (84–85°S, 174–177°W) and were able to identify a potential limit of microbial habitability. While most of the soils we tested contained diverse microbial communities, with fungi being particularly ubiquitous, microbes could not be detected in many of the driest, higher elevation soils—results that were confirmed using cultivation-dependent, cultivation-independent, and metabolic assays. While we cannot confirm that this subset of soils is completely sterile and devoid of microbial life, our results suggest that microbial life is severely restricted in the coldest, driest, and saltiest Antarctic soils. Constant exposure to these conditions for thousands of years has limited microbial communities so that their presence and activity is below detectable limits using a variety of standard methods. Such soils are unlikely to be unique to the studied region with this work supporting previous hypotheses that microbial habitability is constrained by near-continuous exposure to cold, dry, and salty conditions, establishing the environmental conditions that limit microbial life in terrestrial surface soils.This work was supported by grants from the U.S. National Science Foundation (ANT 1341629 to B. J. Adams, N. Fierer, W. Berry Lyons, and D. H. Wall and OPP 1637708 to B. J. Adams) with additional support provided to N. B. Dragone from University Colorado Department of Ecology and Evolutionary Biology

Elevational constraints on the composition and genomic attributes of microbial communities in Antarctic soils

© The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Dragone, N. B., Henley, J. B., Holland-Moritz, H., Diaz, M., Hogg, I. D., Lyons, W. B., Wall, D. H., Adams, B. J., & Fierer, N. Elevational constraints on the composition and genomic attributes of microbial communities in Antarctic soils. Msystems, 7(1), (2022): e01330-21, https://doi.org/10.1128/msystems.01330-21.The inland soils found on the Antarctic continent represent one of the more challenging environments for microbial life on Earth. Nevertheless, Antarctic soils harbor unique bacterial and archaeal (prokaryotic) communities able to cope with extremely cold and dry conditions. These communities are not homogeneous, and the taxonomic composition and functional capabilities (genomic attributes) of these communities across environmental gradients remain largely undetermined. We analyzed the prokaryotic communities in soil samples collected from across the Shackleton Glacier region of Antarctica by coupling quantitative PCR, marker gene amplicon sequencing, and shotgun metagenomic sequencing. We found that elevation was the dominant factor explaining differences in the structures of the soil prokaryotic communities, with the drier and saltier soils found at higher elevations harboring less diverse communities and unique assemblages of cooccurring taxa. The higher-elevation soil communities also had lower maximum potential growth rates (as inferred from metagenome-based estimates of codon usage bias) and an overrepresentation of genes associated with trace gas metabolism. Together, these results highlight the utility of assessing community shifts across pronounced environmental gradients to improve our understanding of the microbial diversity found in Antarctic soils and the strategies used by soil microbes to persist at the limits of habitability.Geospatial support for this work was provided by the Polar Geospatial Center under NSF-OPP awards 1043681 and 155969. This work was supported by grants from the U.S. National Science Foundation Office of Polar Programs (1341629, 1341629, 1341736, and 1637708 to B.J.A., N.F., W.B.L., and D.H.W.), with additional support provided to N.B.D. from the University of Colorado Department of Ecology and Evolutionary Biology