Resonance Raman spectra of wurtzite and zincblende CdSe nanocrystals

Resonance Raman spectra and absolute differential Raman cross-sections have been measured for CdSe nanocrystals in both the wurtzite and zincblende crystal forms at four excitation wavelengths from 457.9 to 514.5 nm. The frequency and bandshape of the longitudinal optical (LO) phonon fundamental is essentially identical for both crystal forms at each excitation wavelength. The LO phonon overtone to fundamental intensity ratio appears to be slightly higher for the wurtzite form, which may suggest slightly stronger exciton-phonon coupling from the Fröhlich mechanism in the wurtzite form. The LO fundamental Raman cross-sections are very similar for both crystal forms at each excitation wavelength. © 2012 Elsevier B.V. All rights reserved

Neural crest migration is driven by a few trailblazer cells with a unique molecular signature narrowly confined to the invasive front

Neural crest (NC) cell migration is crucial to the formation of peripheral tissues during vertebrate development. However, how NC cells respond to different microenvironments to maintain persistence of direction and cohesion in multicellular streams remains unclear. To address this, we profiled eight subregions of a typical cranial NC cell migratory stream. Hierarchical clustering showed significant differences in the expression profiles of the lead three subregions compared with newly emerged cells. Multiplexed imaging of mRNA expression using fluorescent hybridization chain reaction (HCR) quantitatively confirmed the expression profiles of lead cells. Computational modeling predicted that a small fraction of lead cells that detect directional information is optimal for successful stream migration. Single-cell profiling then revealed a unique molecular signature that is consistent and stable over time in a subset of lead cells within the most advanced portion of the migratory front, which we term trailblazers. Model simulations that forced a lead cell behavior in the trailing subpopulation predicted cell bunching near the migratory domain entrance. Misexpression of the trailblazer molecular signature by perturbation of two upstream transcription factors agreed with the in silico prediction and showed alterations to NC cell migration distance and stream shape. These data are the first to characterize the molecular diversity within an NC cell migratory stream and offer insights into how molecular patterns are transduced into cell behaviors

Health literacy, health status, and healthcare utilization of Taiwanese adults: results from a national survey

Abstract Background Low health literacy is considered a worldwide health threat. The purpose of this study is to assess the prevalence and socio-demographic covariates of low health literacy in Taiwanese adults and to investigate the relationships between health literacy and health status and health care utilization. Methods A national survey of 1493 adults was conducted in 2008. Health literacy was measured using the Mandarin Health Literacy Scale. Health status was measured based on self-rated physical and mental health. Health care utilization was measured based on self-reported outpatient clinic visits, emergency room visits, and hospitalizations. Results Approximately thirty percent of adults were found to have low (inadequate or marginal) health literacy. They tended to be older, have fewer years of schooling, lower household income, and reside in less populated areas. Inadequate health literacy was associated with poorer mental health (OR, 0.57; 95% CI, 0.35-0.91). No association was found between health literacy and health care utilization even after adjusting for other covariates. Conclusions Low (inadequate and marginal) health literacy is prevalent in Taiwan. High prevalence of low health literacy is not necessarily indicative of the need for interventions. Systematic efforts to evaluate the impact of low health literacy on health outcomes in other countries would help to illuminate features of health care delivery and financing systems that may mitigate the adverse health effects of low health literacy.http://deepblue.lib.umich.edu/bitstream/2027.42/78252/1/1471-2458-10-614.xmlhttp://deepblue.lib.umich.edu/bitstream/2027.42/78252/2/1471-2458-10-614.pdfPeer Reviewe

Beyond Correlation Filters: Learning Continuous Convolution Operators for Visual Tracking

Discriminative Correlation Filters (DCF) have demonstrated excellent performance for visual object tracking. The key to their success is the ability to efficiently exploit available negative data by including all shifted versions of a training sample. However, the underlying DCF formulation is restricted to single-resolution feature maps, significantly limiting its potential. In this paper, we go beyond the conventional DCF framework and introduce a novel formulation for training continuous convolution filters. We employ an implicit interpolation model to pose the learning problem in the continuous spatial domain. Our proposed formulation enables efficient integration of multi-resolution deep feature maps, leading to superior results on three object tracking benchmarks: OTB-2015 (+5.1% in mean OP), Temple-Color (+4.6% in mean OP), and VOT2015 (20% relative reduction in failure rate). Additionally, our approach is capable of sub-pixel localization, crucial for the task of accurate feature point tracking. We also demonstrate the effectiveness of our learning formulation in extensive feature point tracking experiments. Code and supplementary material are available at http://www.cvl.isy.liu.se/research/objrec/visualtracking/conttrack/index.html.Comment: Accepted at ECCV 201

Determination of urban volatile organic compound emission ratios and comparison with an emissions database

During the NEAQS-ITCT2k4 campaign in New England, anthropogenic VOCs and CO were measured downwind from New York City and Boston. The emission ratios of VOCs relative to CO and acetylene were calculated using a method in which the ratio of a VOC with acetylene is plotted versus the photochemical age. The intercept at the photochemical age of zero gives the emission ratio. The so determined emission ratios were compared to other measurement sets, including data from the same location in 2002, canister samples collected inside New York City and Boston, aircraft measurements from Los Angeles in 2002, and the average urban composition of 39 U.S. cities. All the measurements generally agree within a factor of two. The measured emission ratios also agree for most compounds within a factor of two with vehicle exhaust data indicating that a major source of VOCs in urban areas is automobiles. A comparison with an anthropogenic emission database shows less agreement. Especially large discrepancies were found for the C2-C4 alkanes and most oxygenated species. As an example, the database overestimated toluene by almost a factor of three, which caused an air quality forecast model (WRF-CHEM) using this database to overpredict the toluene mixing ratio by a factor of 2.5 as well. On the other hand, the overall reactivity of the measured species and the reactivity of the same compounds in the emission database were found to agree within 30%. Copyright 2007 by the American Geophysical Union

SEX-SPECIFIC MUSCULOSKELETAL ASYMMETRIES FOLLOWING ANTERIOR CRUCIATE LIGAMENT RECONSTRUCTION

Olivia A. Anderson 1, Allen L. Redinger1, Emery L. Mintz 2,3, Caleb C. Voskuil 2, Jason A. Mogonye 3,4, Joshua C. Carr 2,3 & Breanne S. Baker 1 1 Oklahoma State University, Stillwater, Oklahoma; 2 Texas Christian University, Fort Worth, Texas; 3 Texas Christian University School of Medicine, Fort Worth, Texas; 4 JPS Health Network, Fort Worth, Texas Females are predisposed to more risk factors for primary and secondary anterior cruciate ligament (ACL) injury. Factors such as lower-body bone, muscle, and fat tissue asymmetries post-ACL reconstruction (ACLR) influence recovery; however, if these asymmetries are sex-specific is unknown. PURPOSE: This study aimed to evaluate the influence of sex on musculoskeletal asymmetries and perception of knee function post-ACLR. METHODS: Females (n=11) and males (n=11) with a history of ACLR provided voluntary informed consent prior to completing Dual-energy X-ray Absorptiometry (DXA) scans to measure lower-body bone mineral content (BMC), Fat %, and lean mass (LM), additionally inter-limb asymmetry indices (AI%) were calculated for each tissue. Perceptions of knee function were captured via the International Knee Documentation Committee (IKDC) instrument and participants provided surgical information such as time since surgery and graft type used. Initial Independent t-tests were used to assess sex differences for all variables; however, time since surgery was included as a clinically significant covariate for subsequent analyses. Additionally, Pearson’s Correlations Coefficients evaluated the association between AI% and IKDC scores. Lastly, Cohen’s effect sizes (d) were calculated and α=0.05. RESULTS: Females had greater asymmetries for BMC and LM and lower IKDC scores compared to males post-ACLR (adjusted p≤0.027, d=0.40–0.74). Furthermore, a sex-dependent correlation was observed as tissue asymmetries were negatively correlated with IKDC scores for only females (all r≥0.755; p≤0.007). CONCLUSIONS: These data suggested females with a history of ACLR have greater musculoskeletal tissue asymmetries compared to males, which are associated with lower perceptions of knee function. As such, sex-specific ACLR rehabilitation and musculoskeletal monitoring protocols may need to be developed as we aim to reduce females’ elevated risk for ACL reinjury

Motivated proteins: a web application for studying small three-dimensional protein motifs

<b>BACKGROUND:</b> Small loop-shaped motifs are common constituents of the three-dimensional structure of proteins. Typically they comprise between three and seven amino acid residues, and are defined by a combination of dihedral angles and hydrogen bonding partners. The most abundant of these are alphabeta-motifs, asx-motifs, asx-turns, beta-bulges, beta-bulge loops, beta-turns, nests, niches, Schellmann loops, ST-motifs, ST-staples and ST-turns.We have constructed a database of such motifs from a range of high-quality protein structures and built a web application as a visual interface to this. <b>DESCRIPTION:</b> The web application, Motivated Proteins, provides access to these 12 motifs (with 48 sub-categories) in a database of over 400 representative proteins. Queries can be made for specific categories or sub-categories of motif, motifs in the vicinity of ligands, motifs which include part of an enzyme active site, overlapping motifs, or motifs which include a particular amino acid sequence. Individual proteins can be specified, or, where appropriate, motifs for all proteins listed. The results of queries are presented in textual form as an (X)HTML table, and may be saved as parsable plain text or XML. Motifs can be viewed and manipulated either individually or in the context of the protein in the Jmol applet structural viewer. Cartoons of the motifs imposed on a linear representation of protein secondary structure are also provided. Summary information for the motifs is available, as are histograms of amino acid distribution, and graphs of dihedral angles at individual positions in the motifs. <b>CONCLUSION:</b> Motivated Proteins is a publicly and freely accessible web application that enables protein scientists to study small three-dimensional motifs without requiring knowledge of either Structured Query Language or the underlying database schem

Consenting to health record linkage: evidence from a multi-purpose longitudinal survey of a general population

Background: The British Household Panel Survey (BHPS) is the first long-running UK longitudinal survey with a non-medical focus and a sample covering the whole age range to have asked for permission to link to a range of administrative health records. This study determines whether informed consent led to selection bias and reflects on the value of the BHPS linked with health records for epidemiological research. Methods. Multivariate logistical regression is used, with whether the respondent gave consent to data linkage or not as the dependent variable. Independent variables were entered as four blocks; (i) a set of standard demographics likely to be found in most health registration data, (ii) a broader set of socio-economic characteristics, (iii) a set of indicators of health conditions and (iv) information about the use of health services. Results: Participants aged 16-24, males and those living in England were more likely to consent. Consent is not biased with respect to socio-economic characteristics or health. Recent users of GP services are underrepresented among consenters. Conclusions: Whilst data could only be linked for a minority of BHPS participants, the BHPS offers a great range of information on people's life histories, their attitudes and behaviours making it an invaluable source for epidemiological research. © 2012 Knies et al; licensee BioMed Central Ltd

Meridional Overturning Circulation in a multi-basin model. Part II: Sensitivity to diffusivity and wind in warm and cool climates

This is the final version. Available from the American Meteorological Society via the DOI in this record. The response of the meridional overturning circulation (MOC) to changes in Southern Ocean (SO) zonal wind forcing and Pacific basin vertical diffusivity is investigated under varying buoyancy forcings, corresponding to ‘warm’, ‘present-day’ and ‘cold’ states, in a two-basin general circulation model connected by a southern circumpolar channel. We find that the Atlantic MOC (AMOC) strengthens with increased SO wind stress or diffusivity in the model Pacific, under all buoyancy forcings. The sensitivity of the AMOC to wind stress increases as the buoyancy forcing is varied from a warm to a present-day or cold state, whereas it is most sensitive to the Pacific diffusivity in a present-day or warm state. Similarly, the AMOC is more sensitive to buoyancy forcing over the Southern Ocean under reduced wind stress or enhanced Pacific diffusivity. These results arise because of the increased importance of the Pacific pathway in the warmer climates, giving an increased linkage between the basins and so the opportunity for the diffusivity in the Pacific to affect the overturning in the Atlantic. In cooler states, such as in glacial climates, the two basins are largely decoupled and the wind strength over the SO is the primary determinant of the AMOC strength. Both wind- and diffusively-driven upwelling sustain the AMOC in the warmer (present-day) state. Changes in SO wind stress alone do not shoal the AMOC to resemble that observed at the last glacial maximum; changes in the buoyancy forcing are also needed to decouple the two basins.Natural Environment Research Council (NERC)Royal Societ

Meridional Overturning Circulation in a Multibasin Model. Part I: Dependence on Southern Ocean Buoyancy Forcing

This is the final version. Available on open access from the American Meteorological Society via the DOI in this recordThe variation in the strength and structure of the overturning circulation under varying Southern Ocean buoyancy forcing, corresponding to present day, a cooler (glacial) state, and a possible future warmer state is analyzed in an idealized two-basin general circulation model connected by a southern circumpolar channel. A connection between the North Atlantic Deep Water (NADW) cell in the Atlantic basin and the Pacific Deep Water (PDW) cell in the Pacific basin occurs with a direct flow of NADW into the channel’s lower cell, while PDW upwelled in the Pacific basin can flow directly into the upper wind-driven cell in the channel. The intersection of these cells along with direct zonal flows between the basins completes the interbasin circulation. The present-day Atlantic meridional overturning circulation (AMOC) in the model is upwelled both by wind-driven upwelling in the Southern Ocean and by diffusion in the Pacific and Atlantic. In a cooler climate with enhanced sea ice, the NADW cell shoals, which can then no longer flow directly into the channel’s lower cell, reducing the Pacific pathway of NADW. This leads to a substantial weakening of the AMOC, suggesting buoyancy forcing changes can play a substantial role in the transition of the AMOC to a glacial state. In contrast, in a warmer equilibrium climate state with reduced AABW formation, the NADW cell strengthens and deepens. NADW is increasingly directed along the Pacific pathway, while the direct upwelling in the channel’s wind-driven upper cell plays a smaller role.Leverhulme TrustRoyal Societ