29 research outputs found
Photosynthesis down-regulation precedes carbohydrate accumulation under sink limitation in Citrus
Photosynthesis down-regulation due to an imbalance between sources and sinks in Citrus leaves could be mediated by excessive accumulation of carbohydrates. However, there is limited understanding of the physiological role of soluble and insoluble carbohydrates in photosynthesis regulation and the elements triggering the down-regulation process. In this work, the role of non-structural carbohydrates in the regulation of photosynthesis under a broad spectrum of source-sink relationships has been investigated in the Salustiana sweet orange. Soluble sugar and starch accumulation in leaves, induced by girdling experiments, did not induce down-regulation of the photosynthetic rate in the presence of sinks (fruits). The leaf-to-fruit ratio did not modulate photosynthesis but allocation of photoassimilates to the fruits. The lack of strong sink activity led to a decrease in the photosynthetic rate and starch accumulation in leaves. However, photosynthesis down-regulation due to an excess of total soluble sugars or starch was discarded because photosynthesis and stomatal conductance reduction occurred prior to any significant accumulation of these carbohydrates. Gas exchange and fluorescence parameters suggested biochemical limitations to photosynthesis. In addition, the expression of carbon metabolism-related genes was altered within 24 h when strong sinks were removed. Sucrose synthesis and export genes were inhibited, whereas the expression of ADP-glucose pyrophosphorylase was increased to cope with the excess of assimilates. In conclusion, changes in starch and soluble sugar turnover, but not sugar content per se, could provide the signal for photosynthesis regulation. In these conditions, non-stomatal limitations strongly inhibited the photosynthetic rate prior to any significant increase in carbohydrate levels. © The Author 2011. Published by Oxford University Press. All rights reserved.This work was supported by the Conselleria de Cultura, Educacio i Esport de la Generalitat Valenciana [GV/2007/213 and GV/2009/034].González Nebauer, S.; Renau Morata, B.; Guardiola, JL.; Molina Romero, RV. (2011). Photosynthesis down-regulation precedes carbohydrate accumulation under sink limitation in Citrus. Tree Physiology. 31(2):169-177. doi:10.1093/treephys/tpq103S16917731
DNA methylation changes in triticale due to in vitro culture plant regeneration and consecutive reproduction
ASSESSMENT OF GENETIC STABILITY OF MICROPROPAGATED Eucalyptus globulus Labill HYBRID CLONES BY MEANS OF FLOW CYTOMETRY AND MICROSATELLITES MARKERS
Variabilité génétique des populations tunisiennes de Myrtus communis L. (Myrtaceae) estimée par des marqueurs isoenzymatiques et moléculaires (RAPD)
Identification and characterization of nuclear microsatellites in Mediterranean cedars (Cedrus sp.)
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Genetic diversity and structure of natural and managed populations of Cedrus atlantica (Pinaceae) assessed using random amplified polymorphic DNA
Cedrus atlantica (Pinaceae) is a large and exceptionally long-lived conifer native to the Rif and Atlas Mountains of North Africa. To assess levels and patterns of genetic diversity of this species. samples were obtained throughout the natural range in Morocco and from a forest plantation in Arbucies, Girona (Spain) and analyzed using RAPD markers. Within-population genetic diversity was high and comparable to that revealed by isozymes. Managed populations harbored levels of genetic variation similar to those found in their natural counterparts. Genotypic analyses Of Molecular variance (AMOVA) found that most variation was within populations. but significant differentiation was also found between populations. particularly in Morocco. Bayesian estimates of F,, corroborated the AMOVA partitioning and provided evidence for Population differentiation in C. atlantica. Both distance- and Bayesian-based Clustering methods revealed that Moroccan populations comprise two genetically distinct groups. Within each group, estimates of population differentiation were close to those previously reported in other gymnosperms. These results are interpreted in the context of the postglacial history of the species and human impact. The high degree of among-group differentiation recorded here highlights the need for additional conservation measures for some Moroccan Populations of C. atlantica
Genetic Diversity and Differentiation of Juniperus thurifera in Spain and Morocco as Determined by SSR
Juniperus thurifera L. is an important tree endemic to the western Mediterranean basin that it is able to grow in semi-arid
climates. It nowadays exhibits a disjunct distribution pattern, occurring in North Africa, Spain, France and the Italian Alps.
The Strait of Gibraltar has acted as an efficient barrier against gene flow between African and European populations, which
are considered different subspecies by some authors. We aimed at describing the intraspecific genetic diversity of J.
thurifera in populations from the Iberian Peninsula and Morocco and the phylogeographical relationships among these
populations. The ploidy level of J. thurifera was examined and eleven nuclear microsatellites (nSSRs) developed for J.
thurifera were assessed for genotyping this species. Six nSSRs were polymorphic and subsequently used to assess the
genetic diversity and structure of the studied populations. Genotyping of the tetraploid J. thurifera using nuclear
microsatellites supports the separation of Moroccan and Spanish populations into two genetically differentiated groups that
correspond to the proposed subspecies africana and thurifera. High values of within population genetic diversity were
found, that accounted for 90% of the total genetic variance, while population structure was weak. The estimators of genetic
diversity were higher in populations of Spain than in populations of Morocco pointing for a possible loss of genetic diversity
during the spread of this species to Africa from Europe