Solid-amorphous transition is related to the waterlike anomalies in a fluid without liquid-liquid phase transition

The most accepted origin for the water anomalous behavior is the phase transition between two liquids (LLPT) in the supercooled regime connected to the glassy first order phase transition at lower temperatures. Two length scales potentials are an effective approach that have long being employed to understand the properties of fluids with waterlike anomalies and, more recently, the behavior of colloids and nanoparticles. These potentials can be parameterized to have distinct shapes, as a pure repulsive ramp, such as the model proposed by de Oliveira et al. [J. Chem. Phys. 124, 64901 (2006)]. This model has waterlike anomalies despite the absence of LLPT. To unravel how the waterlike anomalies are connected to the solid phases we employ Molecular Dynamics simulations. We have analyzed the fluid-solid transition under cooling, with two solid crystalline phases, BCC and HCP, and two amorphous regions being observed. We show how the competition between the scales creates an amorphous cluster in the BCC crystal that leads to the amorphization at low temperatures. A similar mechanism is found in the fluid phase, with the system changing from a BCC-like to an amorphous-like structure in the point where a maxima in $k_T$ is observed. With this, we can relate the competition between two fluid structures with the amorphous clusterization in the BCC phase.Those findings help to understand the origins of waterlike behavior in systems without liquid-liquid critical point

Diffusion Enhancement in Core-softened fluid confined in nanotubes

We study the effect of confinement in the dynamical behavior of a core-softened fluid. The fluid is modeled as a two length scales potential. This potential in the bulk reproduces the anomalous behavior observed in the density and in the diffusion of liquid water. A series of $NpT$ Molecular Dynamics simulations for this two length scales fluid confined in a nanotube were performed. We obtain that the diffusion coefficient increases with the increase of the nanotube radius for wide channels as expected for normal fluids. However, for narrow channels, the confinement shows an enhancement in the diffusion coefficient when the nanotube radius decreases. This behavior, observed for water, is explained in the framework of the two length scales potential.Comment: 17 pages, 8 figures, accept for publication at J. Chem. Phy

LEAP Works! Outcomes of a Family-Based Nutrition Education and Physical Activity Promotion Program

Overweight is an endemic public health concern for children, adolescents, and adults. Reducing the prevalence of childhood overweight is a national health objective and nine million U.S. children, currently classified as overweight, require effective nutrition education and physical activity promotion services. The intent of this study was to evaluate the effectiveness of a family-based nutrition education and physical activity promotion program, Lifelong Eating and Activity Patterns (LEAP), as an effective approach for the prevention and treatment of child overweight. The study design was an eight-week intervention and participant files were analyzed retrospectively to ascertain information for the study variables. The main outcome measures were changes in body mass index (BMI), percent body fat, fitness level, and eating and activity behaviors for child and parent participants. Qualitative data gathered from an exit survey for both children and parents were used to assess perceived benefits, value, and effectiveness of the program. Participants included 107 boys (n=48) and girls (n=59) ages 7-14, mean age 10.6 years, and their accompanying parents. The child participants were either “at risk for overweight” (3%) or “overweight” (97%). The results showed a significant decrease in BMI for both child and parent participants and percent body fat for child participants. There was a significant increase in reported fruit and vegetable consumption, regular physical activity, and in fitness parameters for both child and parent participants. The qualitative exit survey results indicated that the LEAP program resulted in significant improvements in knowledge, attitude, and behavior. In conclusion, this family-based nutrition education and physical activity promotion program was effective in decreasing BMI and body fat, increasing fitness level, and eliciting positive changes in eating and activity attitudes and behavior in both child and parent participants

Plasminogen activator inhibitor-1 and type 2 diabetes: a systematic review and meta-analysis of observational studies

An emerging body of evidence has implicated plasminogen activator inhibitor-1 (PAI-1) in the development of type 2 diabetes (T2D), though findings have not always been consistent. We systematically reviewed epidemiological studies examining the association of PAI-1 with T2D. EMBASE, PubMed, Web of Science, and the Cochrane Library were searched to identify studies for inclusion. Fifty-two studies (44 cross-sectional with 47 unique analytical comparisons and 8 prospective) were included. In pooled random-effects analyses of prospective studies, a comparison of the top third vs. bottom third of baseline PAI-1 values generated a RR of T2D of 1.67 (95% CI 1.28-2.18) with moderate heterogeneity (I-2 = 38%). Additionally, of 47 cross-sectional comparisons, 34(72%) reported significantly elevated PAI-1 among diabetes cases versus controls, 2(4%) reported significantly elevated PAI-1 among controls, and 11(24%) reported null effects. Results from pooled analyses of prospective studies did not differ substantially by study design, length of follow-up, adjustment for various putative confounding factors, or study quality, and were robust to sensitivity analyses. Findings from this systematic review of the available epidemiological literature support a link between PAI-1 and T2D, independent of established diabetes risk factors. Given the moderate size of the association and heterogeneity across studies, future prospective studies are warranted

LEAP Works! Outcomes of a Family-based Nutrition Education and Physical Activity Promotion Program

Abstract Overweight is an endemic public health concern for children, adolescents, and adults. Reducing the prevalence of childhood overweight is a national health objective and nine million U.S. children, currently classified as overweight, require effective nutrition education and physical activity promotion services. The intent of this study was to evaluate the effectiveness of a family-based nutrition education and physical activity promotion program, Lifelong Eating and Activity Patterns (LEAP), as an effective approach for the prevention and treatment of child overweight. The study design was an eight-week intervention and participant files were analyzed retrospectively to ascertain information for the study variables. The main outcome measures were changes in body mass index (BMI), percent body fat, fitness level, and eating and activity behaviors for child and parent participants. Qualitative data gathered from an exit survey for both children and parents were used to assess perceived benefits, value, and effectiveness of the program. Participants included 107 boys (n=48) and girls (n=59) ages 7-14, mean age 10.6 years, and their accompanying parents. The child participants were either "at risk for overweight" (3%) or "overweight" (97%). The results showed a significant decrease in BMI for both child and parent participants and percent body fat for child participants. There was a significant increase in reported fruit and vegetable consumption, regular physical activity, and in fitness parameters for both child and parent participants. The qualitative exit survey results indicated that the LEAP program resulted in significant improvements in knowledge, attitude, and behavior. In conclusion, this family-based nutrition education and physical activity promotion program was effective in decreasing BMI and body fat, increasing fitness level, and eliciting positive changes in eating and activity attitudes and behavior in both child and parent participants

Knowledge, attitudes and practices about vaccination in Trentino, Italy in 2019

Vaccination is among the most important areas of progress in the worldwide history of public health. However, a crescent wave of anti-vaccine groups has grown in Western countries, especially in Italy, in the last two decades. Our aim was to evaluate adult's hesitancy and knowledge about vaccines and related diseases in Trentino-Alto Adige -the Italian region with the lowest vaccination coverages

Human neutrophil alloantigens systems

Neutrophil alloantigens are involved in a variety of clinical conditions including immune neutropenias, transfusion-related acute lung injury (TRALI), refractoriness to granulocyte transfusions and febrile transfusion reactions. In the last decade, considerable progress has been made in the characterization of the implicated antigens. Currently, seven antigens are assigned to five human neutrophil antigen (HNA) systems. The HNA-1a, HNA-1b and HNA-1c antigens have been identified as polymorphic forms of the neutrophil Fcγ receptor IIIb (CD16b), encoded by three alleles. Recently, the primary structure of the HNA-2a antigen was elucidated and the HNA-2a-bearing glycoprotein was identified as a member of the Ly-6/uPAR superfamily, which has been clustered as CD177. The HNA-3a antigen is located on a 70-95 kDa glycoprotein; however, its molecular basis is still unknown. Finally, the HNA-4a and HNA-5a antigens were found to be caused by single nucleotide mutations in the αM (CD11b) and αL (CD11a) subunits of the leucocyte adhesion molecules (β2 integrins). Molecular and biochemical characterization of neutrophil antigenshave expanded our diagnostic tools by the introduction of genotyping techniques and immunoassays for antibody identification. Further studies in the field of neutrophil immunology will facilitate the prevention and management of transfusion reactions and immune diseases caused by neutrophil antibodies.Os aloantígenos de neutrófilos estão associados a várias condições clínicas como neutropenias imunes, insuficiência pulmonar relacionada à transfusão (TRALI), refratariedade à transfusão de granulócitos, e reações transfusionais febris. Na última década, foi observado considerável progresso na caracterização dos aloantígenos envolvidos nestas condições clínicas. Atualmente sete antígenos estão incluídos em cinco sistemas de antígenos de neutrófilo humano (HNA). Os antígenos HNA-1a, HNA-1b e HNA-1c foram identificados como formas polimórficas do receptor Fcγ RIIIb (CD16b), codificados por três alelos. Recentemente, a estrutura primária do antígeno HNA-2a foi elucidada e a glicoproteína carreadora do antígeno foi identificada como um membro da superfamília Ly-6/uPARe designada como CD177. O antígeno HNA-3a está localizadoem uma glicoproteína de 70-90 kDa, entretanto sua base molecular ainda é desconhecida. Finalmente, os antígenos HNA-4ae HNA-5a são resultantes de mutações de um único nucleotídeo nas subunidades αM (CD11b) and αL (CD11a) das moléculas de adesão de leucócitos (β2 integrinas). A caracterização molecular e bioquímica dos antígenos neutrofílicos permitiu a expansão das ferramentas diagnósticas pela introdução de técnicas de genotipagem e imunoensaios para a identificação de anticorpos. Novos estudos envolvendo a imunologia de granulócitos serão de grande valor para a prevenção e tratamento de reações transfusionais e doenças imunes causadas por aloanticorpos de neutrófilos.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Federal de São Paulo (UNIFESP)UNIFESPSciEL