Preserving and promoting the health of university students

Currently, the health of the young population is a serious problem. The number of students with chronic diseases of various etiologies increases annually. The article discusses the methods and conditions aimed at improving the adaptation processes to increase physical stress.В настоящее время здоровье молодого населения представляет собой серьезную проблему. Ежегодно увеличивается количество студентов, имеющих хронические заболевания различной этиологии. В статье рассматриваются методы и условия, направленные на совершенствование адаптационных процессов к увеличению физического напряжения

Concordance study between the ParaDNA® Intelligence Test, a rapid DNA profiling assay, and a conventional STR typing kit (AmpFlSTR® SGM Plus®).

The ParaDNA® Intelligence Test enables STR profiling directly from human biological samples and evidence items collected from crime scene in 75min. Designed for non-expert use this system allows DNA information to be available to investigators before it would typically be available from a laboratory. The ParaDNA Intelligence Test system amplifies D3S1358, D8S119, D16S539, D18S1358 and TH01 STR loci and the gender typing locus amelogenin and detects the alleles present with HyBeacon® probes. Individual DNA samples from 381 UK Caucasian individuals were analysed using AmpFlSTR® SGM Plus® and the ParaDNA Intelligence Test with the derived STR profiles compared. Here we describe the high level of concordance demonstrated between the two systems and discuss this with reference to allele frequencies and the discriminatory power offered by the ParaDNA Intelligence Test

ПНЕВМОНІЯ ТА ЕНЦЕФАЛІТ ЯК УСКЛАДНЕННЯ ГОСТРИХ РЕСПІРАТОРНИХ ЗАХВОРЮВАНЬ І ГРИПУ

SUMMARY. The article provides a review of the literatureon complications of influenza and acute respiratoryinfections with pneumonia, encephalitis and theirpossible agents. The description of cases of pneumoniaand encephalitis in the soldier on a background of acuterespiratory disease is illustrated.Key words: pneumonia, encephalitis, military.Наводиться огляд літератури стосовно усклад-нення грипу та гострих респіраторних захворюваньпневмонією, енцефалітом та можливі їх збудники.Приведено опис випадку розвитку пневмонії та енце-фаліту у військовослужбовця на фоні гострого респі-раторного захворювання.Ключові слова: пневмонія, енцефаліт, військо-вослужбовці

ODRL Policy Modelling and Compliance Checking

This paper addresses the problem of constructing a policy pipeline that enables compliance checking of business processes against regulatory obligations. Towards this end, we propose an Open Digital Rights Language (ODRL) profile that can be used to capture the semantics of both business policies in the form of sets of required permissions and regulatory requirements in the form of deontic concepts, and present their translation into Answer Set Programming (via the Institutional Action Language (InstAL)) for compliance checking purposes. The result of the compliance checking is either a positive compliance result or an explanation pertaining to the aspects of the policy that are causing the noncompliance. The pipeline is illustrated using two (key) fragments of the General Data Protect Regulation, namely Articles 6 (Lawfulness of processing) and Articles 46 (Transfers subject to appropriate safeguards) and industrially-relevant use cases that involve the specification of sets of permissions that are needed to execute business processes. The core contributions of this paper are the ODRL profile, which is capable of modelling regulatory obligations and business policies, the exercise of modelling elements of GDPR in this semantic formalism, and the operationalisation of the model to demonstrate its capability to support personal data processing compliance checking, and a basis for explaining why the request is deemed compliant or not

Bedside Sublingual Video Imaging of Microcirculation in Assessing Bacterial Infection in Cirrhosis

Bacterial infections are common in cirrhosis and can lead to life-threatening complications. Sidestream dark-field (SDF) imaging has recently emerged as a noninvasive tool for capturing real-time video images of sublingual microcirculation in critically ill patients with sepsis. The objective of this study was to assess the utility of SDF in determining underlying infection in patients with cirrhosis. Sublingual microcirculation was compared among patients with compensated cirrhosis (Group A, n = 13), cirrhosis without sepsis (Group B, n = 18), cirrhosis with sepsis (Group C, n = 14), and sepsis only (Group D, n = 10). The blood flow was semi-quantitatively evaluated in four equal quadrants in small (10–25 mm); medium (26–50 mm); and large (51–100 mm) sublingual capillaries. The blood flow was described as no flow (0), intermittent flow (1), sluggish flow (2), and continuous flow (3). The overall flow score or microvascular flow index (MFI) was measured for quantitative assessment of microcirculation and predicting power for concurrent infection in cirrhosis. Marked impairment was observed at all levels of microvasculature in Groups B and C when compared with Group A. This effect was restricted to small vessels only when Group B was compared with Group C. MFI < 1.5 was found to have highest sensitivity (100%) and specificity (100%) for infection in decompensated cirrhosis. SDF imaging of sublingual microcirculation can be a useful bedside diagnostic tool to assess bacterial infection in cirrhosis

RNA delivery by extracellular vesicles in mammalian cells and its applications.

The term 'extracellular vesicles' refers to a heterogeneous population of vesicular bodies of cellular origin that derive either from the endosomal compartment (exosomes) or as a result of shedding from the plasma membrane (microvesicles, oncosomes and apoptotic bodies). Extracellular vesicles carry a variety of cargo, including RNAs, proteins, lipids and DNA, which can be taken up by other cells, both in the direct vicinity of the source cell and at distant sites in the body via biofluids, and elicit a variety of phenotypic responses. Owing to their unique biology and roles in cell-cell communication, extracellular vesicles have attracted strong interest, which is further enhanced by their potential clinical utility. Because extracellular vesicles derive their cargo from the contents of the cells that produce them, they are attractive sources of biomarkers for a variety of diseases. Furthermore, studies demonstrating phenotypic effects of specific extracellular vesicle-associated cargo on target cells have stoked interest in extracellular vesicles as therapeutic vehicles. There is particularly strong evidence that the RNA cargo of extracellular vesicles can alter recipient cell gene expression and function. During the past decade, extracellular vesicles and their RNA cargo have become better defined, but many aspects of extracellular vesicle biology remain to be elucidated. These include selective cargo loading resulting in substantial differences between the composition of extracellular vesicles and source cells; heterogeneity in extracellular vesicle size and composition; and undefined mechanisms for the uptake of extracellular vesicles into recipient cells and the fates of their cargo. Further progress in unravelling the basic mechanisms of extracellular vesicle biogenesis, transport, and cargo delivery and function is needed for successful clinical implementation. This Review focuses on the current state of knowledge pertaining to packaging, transport and function of RNAs in extracellular vesicles and outlines the progress made thus far towards their clinical applications

Thrombocytopenia in malaria: who cares?

Despite not being a criterion for severe malaria, thrombocytopenia is one of the most common complications of both Plasmodium vivax and Plasmodium falciparum malaria. In a systematic review of the literature, platelet counts under 150,000/mm³ ranged from 24-94% in patients with acute malaria and this frequency was not different between the two major species that affected humans. Minor bleeding is mentioned in case reports of patients with P. vivax infection and may be explained by medullary compensation with the release of mega platelets in the peripheral circulation by megakaryocytes, thus maintaining a good primary haemostasis. The speculated mechanisms leading to thrombocytopenia are: coagulation disturbances, splenomegaly, bone marrow alterations, antibody-mediated platelet destruction, oxidative stress and the role of platelets as cofactors in triggering severe malaria. Data from experimental models are presented and, despite not being rare, there is no clear recommendation on the adequate management of this haematological complication. In most cases, a conservative approach is adopted and platelet counts usually revert to normal ranges a few days after efficacious antimalarial treatment. More studies are needed to specifically clarify if thrombocytopenia is the cause or consequence of the clinical disease spectrum