12 research outputs found
Can sleep and resting behaviours be used as indicators of welfare in shelter dogs (Canis lupusfamiliaris)?
Previous research on humans and animals suggests that the analysis of sleep patterns
may reliably inform us about welfare status, but little research of this kind has been carried
out for non-human animals in an applied context. This study explored the use of sleep and
resting behaviour as indicators of welfare by describing the activity patterns of dogs (Canis
lupus familiaris) housed in rescue shelters, and comparing their sleep patterns to other
behavioural and cognitive measures of welfare. Sleep and activity patterns were observed
over five non-consecutive days in a population of 15 dogs. Subsequently, the characteristics
of sleep and resting behaviour were described and the impact of activity on patterns of
sleep and resting behaviour analysed. Shelter dogs slept for 2.8% of the day, 14.3% less
than previously reported and experienced less sleep fragmentation at night (32 sleep
bouts). There were no statistically significant relationships between behaviours exhibited
during the day and sleep behaviour. A higher proportion of daytime resting behaviour was
significantly associated with a positive judgement bias, less repetitive behaviour and
increased time spent coded as ‘relaxed’ across days by shelter staff. These results suggest
that, in the context of a busy shelter environment, the ability to rest more during the day
could be a sign of improved welfare. Considering the non-linear relationship between sleep
and welfare in humans, the relationship between sleep and behavioural indicators of welfare,
including judgement bias, in shelter dogs may be more complex than this study could
detect
CD8+ T cell-mediated control of distant tumours following local photodynamic therapy is independent of CD4+ T cells and dependent on natural killer cells
Cancer survival rates decrease in the presence of disseminated disease. However, there are few therapies that are effective at eliminating the primary tumour while providing control of distant stage disease. Photodynamic therapy (PDT) is an FDA-approved modality that rapidly eliminates local tumours, resulting in cure of early disease and palliation of advanced disease. Numerous pre-clinical studies have shown that local PDT treatment of tumours enhances anti-tumour immunity. We hypothesised that enhancement of a systemic anti-tumour immune response might control the growth of tumours present outside the treatment field. To test this hypothesis we delivered PDT to subcutaneous (s.c.) tumours of mice bearing both s.c. and lung tumours and monitored the growth of the untreated lung tumours. Our results demonstrate that PDT of murine tumours provided durable inhibition of the growth of untreated lung tumours. The inhibition of the growth of tumours outside the treatment field was tumour-specific and dependent on the presence of CD8+ T cells. This inhibition was accompanied by an increase in splenic anti-tumour cytolytic activity and by an increase in CD8+ T cell infiltration into untreated tumours. Local PDT treatment led to enhanced anti-tumour immune memory that was evident 40 days after tumour treatment and was independent of CD4+ T cells. CD8+ T cell control of the growth of lung tumours present outside the treatment field following PDT was dependent upon the presence of natural killer (NK) cells. These results suggest that local PDT treatment of tumours lead to induction of an anti-tumour immune response capable of controlling the growth of tumours outside the treatment field and indicate that this modality has potential in the treatment of distant stage disease