Conformational studies of various hemoglobins by natural-abundance 13C NMR spectroscopy

Studies of variously liganded hemoglobins (both from human and rabbit) by natural-abundance 13C NMR spectroscopy have revealed apparent conformational differences that have been interpreted on the basis of two quaternary structures for the α2ß2 tetramer, and variable tertiary structures for the individual α and ß subunits. In solution, rabbit hemoglobins appear to have somewhat more flexibility than human hemoglobins

Laserlight visual cueing device for freezing of gait in Parkinson's disease: a case study of the biomechanics involved

AbstractBackground: Freezing of gait (FOG) is a serious gait disorder affecting up to two-thirds of people with Parkinson's disease (PD). Cueing has been explored as a method of generating motor execution using visual transverse lines on the floor. However, the impact of a laser light visual cue remains unclear. Objective: To determine the biomechanical effect of a laser cane on FOG in a participant with PD compared to a healthy age- and gender-matched control. Methods: The participant with PD and healthy control were given a task of initiating gait from standing. Electromyography (EMG) data were collected from the tibialis anterior (TA) and the medial gastrocnemius (GS) muscles using an 8-channel system. A 10-camera system (Qualisys) recorded movement in 6 degrees of freedom and a calibrated anatomical system technique was used to construct a full body model. Center of mass (COM) and center of pressure (COP) were the main outcome measures. Results: The uncued condition showed that separation of COM and COP took longer and was of smaller magnitude than the cued condition. EMG activity revealed prolonged activation of GS, with little to no TA activity. The cued condition showed earlier COM and COP separation. There was reduced fluctuation in GS, with abnormal, early bursts of TA activity. Step length improved in the cued condition compared to the uncued condition. Conclusion: Laserlight visual cueing improved step length beyond a non-cued condition for this patient indicating improved posture and muscle control

Tm3+/Ho3+ codoped tellurite fiber laser

Continuous-wave and Q-switched lasing from a Tm 3+ /Ho 3+ codoped tellurite fiber is reported. An Yb 3+ /Er 3+ -doped silica fiber laser operating at 1.6μm was used as an in-band pump source, exciting the Tm 3+ ions into the F 4 3 level. Energy is then nonradiatively transferred to the upper laser level, the I 7 5 state of Ho 3+ . The laser transition is from the I 7 5 level to the I 8 5 level, and the resulting emission is at 2.1μm . For continuous wave operation, the slope efficiency was 62% and the threshold 0.1W ; the maximum output demonstrated was 0.16W . Mechanical Q switching resulted in a pulse of 0.65μJ energy and 160ns duration at a repetition rate of 19.4kHz

RSRM-11 (36OW011) ballistics mass properties (STS-35)

The propulsion performance and reconstructed mass properties data from Thiolol's RSRM-11 motors which were assigned to the STS-35 launch are contained. The Thiokol manufacturing designations for the motors were 360W011A/360W011B, which are referred to as RSRM-11A and RSRM-B, respectively. The launch of STS-35 occurred on 2 December 1990 at the Eastern Test Range (ETR). The data contained herein was input to the STS-35 Flight Evaluation Report. The SRM propellant, TP-H1148, is a composite type solid propellants, formulated of polybutediene acrylic acid, acryonitrile terpolymer binder, epoxy curing agent, ammonium perchlorate oxidizer, and aluminum powder fuel. A small amount of burning rate catalyst (iron oxide) was added to achieve the desired propellant burn rate. The propellant evaluation and raw material information for the RSRM-11 are included. The ballistic performance presented was based on the Operational Flight Instrumentation (OFI) 12.5 sample per second pressure data for the steady state and tail off portion of the pressure trace. Recent studies have shown that the transducers are affected by the measuring system at KSC and temperature gradients created by the igniter heaters. Therefore, an adjustment to the data from each transducer is made to make the initial reading match the atmospheric pressure at the time of launch

The Efficiencies Defense in Mergers: The Baby-Food Case Reconsidered

The Federal Trade Commission’s successful challenge to the proposed merger of Heinz and Beech-Nut baby food operations in 2001 remains a controversial case that raises concern over the role of cost efficiencies in merger analysis. Although the FTC argued that the merger would result in an increased likelihood of coordinated effects, we develop an alternative explanation for why the merger was likely to harm consumers even in the absence of such cooperation. We show that a conventional model of vertical product differentiation is able to replicate the premerger market data. Vertical product differentiation assumes that consumers agree on the relative quality of different products, which seems to describe the baby food market. When the model is then used to determine potential post-merger outcomes, we find that only using the most favorable assumptions for Heinz, would the claimed cost-efficiencies have been passed on to consumers. Under any more conservative and realistic scenarios, consumer prices rise substantially. The analysis supports the decision to oppose the merger. It also raises some doubt about the merit of cost efficiencies as a merger defense when an industry is characterized by vertical product differentiation

Long-range electron transfer in structurally engineered pentaammineruthenium (histidine-62) cytochrome c

In many biological processes, long-range electron transfer (ET) plays a key role. When the three-dimensional structures of proteins are accurately known, use of modified proteins and protein-protein complexes provides an experimental approach to study ET rates between two metal centers. For Ru(His)- modified proteins, the introduction of histidine residues at any desired surface location by site-directed mutagenesis opens the way for systematic investigations of ET pathways