A virologist’s guide to hide and seek : evasion of innate immunity by primate lentiviruses

HIV is the cause of a chronic, incurable infection in 37 million people worldwide in 2014. This thesis investigates how the immune system detects HIV and how in turn HIV avoids detection by the immune system. The understanding of the viral evasion mechanisms that prevent immune detection (“Hide and Seek”) is important to successfully develop future vaccines and cure strategies for HIV. Primate lentiviruses belong to the retrovirus family and include the human immunodeficiency viruses (HIV-1 and HIV-2) and simian immunodeficiency viruses (SIV). HIV infects cells of the immune system, including subsets of T cells and dendritic cells (DC). Upon cell entry, the detection of the virus by cellular pattern recognition receptors triggers an intracellular cascade of innate antiviral defense mechanisms. In DCs, these mechanisms include the secretion of interferon α and the induction of cellular restriction factors, among these members of the APOBEC3 family that inhibit viral replication. As demonstrated in Paper III, low doses of interferon α protected DCs from HIV-1 infection and limited viral spread from DCs to T cells by inducing an increase in APOBEC3G, F and A expression. DCs are professional antigen presenting cells that present antigen to cells of the innate and adaptive immune system. Invariant natural killer T cells (iNKT) cells are innate T cells that recognize endogenous and exogenous lipid antigens presented by CD1d. Activated iNKT cells regulate the immune response by producing cytokines that recruit and activate innate and adaptive immune cells. Previous studies have shown that the HIV-1 accessory proteins Vpu and Nef interfere with CD1d cell surface expression in infected DCs, thus inhibiting the effective activation of iNKT cells. The results of Paper II demonstrated that infected DCs respond to HIV-1 infection by increasing CD1d surface levels and enhanced presentation of the endogenous lipid GlcCer. This enabled iNKT cell activation by HIV-infected DCs. However, HIV-1 counteracts iNKT cell activation by reducing CD1d cell surface expression using the HIV-1 proteins Nef and Vpu. In Paper I, efforts to elucidate the mechanism of CD1d antagonism by Vpu identified a highly conserved C-terminal APW motif in HIV-1 group M subtype B Vpu proteins that was necessary for CD1d downregulation. Moreover, we identified this immune evasion mechanism to be a conserved function of diverse HIV-1 and related SIV Vpus. These findings emphasize the role of CD1d-mediated immunity in the antiviral defense against HIV-1 and support the need for further studies investigating the therapeutic potential of Vpu inhibition in the future. Previous studies found that innate cellular immune responses are altered in chronic HIV-1 infection. Our results in Paper IV from an occupational cohort in Guinea-Bissau suggest that this is a general phenomenon of chronic HIV infection as NK and iNKT cells were partly lost and the remaining populations displayed elevated activation levels in chronic HIV-1, HIV-2, and dual infections

Software Requirements and Prototype Development of a Web Application for Quality Assurance (QA) in Radiation Therapy - a QAlender

The implementation of a web based portal QA solution will lead to a high acceptance of the staff as the usage of commonly known standard software (e.g. web browser) allows intuitive handling. In the daily use a significant simplification of the workflow and Performance enhancement can be achieved by easy access to the check documents. As the data is now saved in a database it can easily be processed and long-term trends can be displayed. Therefore possible errors can be detected much easier and earlier. By the usage of time stamps and user authentication procedures and user responsibilities are comprehensibly documented. As the software is browser based, integration into an existing software Environment is not critical. As only technical QA data is processed, no further data security measures are necessary. A certification as a medical product is not required

Modelling human choices: MADeM and decision‑making

Research supported by FAPESP 2015/50122-0 and DFG-GRTK 1740/2. RP and AR are also part of the Research, Innovation and Dissemination Center for Neuromathematics FAPESP grant (2013/07699-0). RP is supported by a FAPESP scholarship (2013/25667-8). ACR is partially supported by a CNPq fellowship (grant 306251/2014-0)