Study of Shoot Multiplication of Strawberry (Fragaria ananassa)

Effects of different hormonal concentration on multiple shoot formation of RABI Strawberry-3 were studied. Where the explants were runner tips that cultured on MS basal medium supplemented with 6-Benzyl Adenine and Kinetin. The maximum percentage 80% of shoot was obtained from Benzyl Adenine concentrations of 0.5 mg/l and 1.0 mg/l whereas Kinetin concentrations were 1.5 mg/l and 2.0 mg/l separately. More promising result was found by combining two impressive concentrations of each hormone. When MS basal media was supplemented with Kinetin 2.0mg/l + Benzyl Adenin A 0.5 mg/l, Kinetin 1.5 mg/l + Benzyl Adenine 0.5 mg/l and Kinetin 1.5 mg/l + Benzyl Adenine 1.0 mg/l respectively then 100% shoots were obtained. DOI: http://dx.doi.org/10.3329/ijarit.v1i1-2.13936 Int. J. Agril. Res. Innov. & Tech. 1 (1&2): 69-72, December, 201

An Efficient Irrigation System for Plasticulture of Strawberry in Bangladesh

Plasticulture technique is a commonly used technique for advanced strawberry production. Due to high cost and tough maintenance, this technique is not practiced commercially in Bangladesh. We have found that, if the irrigation system can be switched to a cheaper one with high efficiency, then the cost can be minimized. We applied Micro-drip mediated irrigation system to minimize the cost. Our proposed modification of plasticulture using micro-drip irrigation system was outstanding in all our concerned horticultural parameters like height of the plants, number of leaves, north-south spreading, and east-west spreading of the plants. Paired Samples analysis between conventional and plasticulture procedure, test of significance for the pairs of mean, analysis of variance and LSD test at 5% level of significance were done for statistical analysis to conclude the  result. The statistical analysis has also shown that the plasticulture method proposed by us was highly significant over the conventional technique and practical for the rural farmer of Bangladesh. It reduces the production costs, improves fruit quality and yield, and reduces the need for chemical pesticides. Keywords: Micro drip irrigation, Soil solarization, Mulching, and Fruit Qualit

An efficient callus initiation and direct regeneration of Stevia rebaudiana

The experiment was carried out to establish an efficient callus induction system of Stevia rebaudiana from a variety of explants as well as direct regeneration from nodes. Callus initiation was done to extract secondary metabolites. However direct regeneration is essential for rapid large-scale clonal propagation. Among leaf, node and inter-nodal explants, the best callus initiation performance was observed by nodes. MS medium fortified with α-naphthalene acetic acid (NAA) 2.0 mg/L + 6-benzyladenine (BA) 2.0 mg/L showed the highest (93.33 ± 6.67%) callus induction by nodal explants. Though inter-nodal explants showed a moderate response (73.33 ± 6.67%) for callus induction in MS medium complemented with NAA 3.0 mg/L + BA 1.0 mg/L, nodal explants showed higher response (86.67 ± 13.33%) than inter-nodal explants in that nutritional environment. Leaf explants always showed very poor callus. However, the best direct regenerating medium was MS medium + BA 1.0 mg/L for multiple shoot proliferation and then transferring those shoots to ½ MS medium + NAA 1.0 mg/L for root formation.Key words: In vitro, Stevia rebaudiana, 2,4-D, α-naphthalene acetic acid (NAA), 6-benzyladenine (BA), plant growth regulators

Development of Efficient Callus Initiation of Malta (Citrus sinensis) Through Tissue Culture

The effects of different hormonal concentration on shoot formation and callus induction were studied on BARI Malta-1 (Citrus sinensis). Seeds containing seed coat and without seed coat was treated by Murashige and Skoog (MS) media supplemented with 6-benzyl adenine (BA) and Kinetin (KIN). Removal of seed coat showed an early response for shoot formation. The highest (70%) shoot formation was obtained from seeds without seed coat treated with MS basal media + BA 1.0 mg/l while KIN showed no response for shoot formation in any supplemented concentration. However, in case of callus induction internodes and apical shoot tips were used as explants and 2, 4-dichlorophenoxy acetic acid (2, 4-D) was used as callus inducing hormone. MS basal media supplemented with 2, 4-D, 2.0 mg/l showed highest (68%) callus induction. DOI: http://dx.doi.org/10.3329/ijarit.v1i1-2.13935 Int. J. Agril. Res. Innov. & Tech. 1 (1&2): 64-68, December, 201

Personal protective equipment (PPE) related adverse skin reactions among healthcare workers at the main COVID-19 isolation center in Barbados

Background: The use of personal protective equipment (PPE) reduces the risk of transmission of infectious agents significantly among healthcare workers (HCWs). The study aimed to investigate the prevalence and characteristics of PPE-related adverse skin reactions among HCWs working at the main COVID-19 isolation center in Barbados. Methods: A cross-sectional web-based online survey was conducted during April to June 2021 which recorded demographic information, details of PPE use and adverse skin reactions including severity and duration of onset of symptoms. Results: Most of the respondents used PPE for consecutive days (77.9%), 1–6 h/day (59.2%), and more than a year (62.5%). Fewer than half of the participants (45.6%) experienced adverse skin reactions from the use of PPE. The reactions were mostly observed in the cheeks (40.4%) and nose bridges (35.6%). Females had more reactions than their male counterparts (p = 0.003). The use of N95 masks and a combination of surgical and N95 masks produced adverse effects predominantly in the ears (60%) and cheeks (56.4%). Binary logistic regression showed that female HCWs (OR = 5.720 95% CI: 1.631, 20.063), doctors (OR = 5.215 95% CI: 0.877, 31.002), and longer duration of PPE use (>1 year) (OR = 2.902 95% CI: 0.958, 8.787) caused a significantly higher prevalence of adverse skin reactions. Conclusion: The PPE-related skin reactions were common among HCWs which mainly occurred due to prolonged use. Preventive measures inclusive of appropriate training of HCWs on the use of PPE are recommended to minimize these adverse event

Development of efficient callus initiation of malta (Citrus sinensis) through tissue culture

The effects of different hormonal concentration on shoot formation and callus induction were studied on BARI Malta-1 (Citrus sinensis). Seeds containing seed coat and without seed coat was treated by Murashige and Skoog (MS) media supplemented with 6-benzyl adenine (BA) and Kinetin (KIN). Removal of seed coat showed an early response for shoot formation. The highest (70%) shoot formation was obtained from seeds without seed coat treated with MS basal media + BA 1.0 mg/l while KIN showed no response for shoot formation in any supplemented concentration. However, in case of callus induction internodes and apical shoot tips were used as explants and 2, 4-dichlorophenoxy acetic acid (2, 4-D) was used as callus inducing hormone. MS basal media supplemented with 2, 4-D, 2.0 mg/l showed highest (68%) callus induction

A model driven approach to the design of a gamified e-learning system for clinical guidelines

Clinical practice guidelines (CPGs) are indispensable in the practice of evidence-based medicine. However, the cost of effective CPG dissemination strategies is prohibitive and not cost-effective. Therefore, scalable strategies using available technology are needed. We describe a formal model-driven approach to design a gamified e-learning system for clinical guidelines. We employ gamification to increase user motivation and engagement in the training of guideline content. Our approach involves the use of models for different aspects of the system, an entity model for the clinical domain, a workflow model for the clinical processes and a game model to manage the training sessions. A game engine instantiates a training session by coupling the workflow and entity models to automatically generate questions based on the data in the model instances. Our approach is flexible and adaptive as it allows for easy updates of the guidelines, integration with different device interfaces and representation of any guideline

STUDY OF SHOOT MULTIPLICATION OF STRAWBERRY (Fragaria ananassa)

Effects of different hormonal concentration on multiple shoot formation of RABI Strawberry-3 were studied. Where the explants were runner tips that cultured on MS basal medium supplemented with 6-Benzyl Adenine and Kinetin. The maximum percentage 80% of shoot was obtained from Benzyl Adenine concentrations of 0.5 mg/l and 1.0 mg/l whereas Kinetin concentrations were 1.5 mg/l and 2.0 mg/l separately. More promising result was found by combining two impressive concentrations of each hormone. When MS basal media was supplemented with Kinetin 2.0mg/l + Benzyl Adenin A 0.5 mg/l, Kinetin 1.5 mg/l + Benzyl Adenine 0.5 mg/l and Kinetin 1.5 mg/l + Benzyl Adenine 1.0 mg/l respectively then 100% shoots were obtained

Study of Shoot Multiplication of Strawberry (Fragaria ananassa)

Effects of different hormonal concentration on multiple shoot formation of RABI Strawberry-3 were studied. Where the explants were runner tips that cultured on MS basal medium supplemented with 6-Benzyl Adenine and Kinetin. The maximum percentage 80% of shoot was obtained from Benzyl Adenine concentrations of 0.5 mg/l and 1.0 mg/l whereas Kinetin concentrations were 1.5 mg/l and 2.0 mg/l separately. More promising result was found by combining two impressive concentrations of each hormone. When MS basal media was supplemented with Kinetin 2.0mg/l + Benzyl Adenin A 0.5 mg/l, Kinetin 1.5 mg/l + Benzyl Adenine 0.5 mg/l and Kinetin 1.5 mg/l + Benzyl Adenine 1.0 mg/l respectively then 100% shoots were obtained. DOI: http://dx.doi.org/10.3329/ijarit.v1i1-2.13936 Int. J. Agril. Res. Innov. & Tech. 1 (1&2): 69-72, December, 201

A model driven approach to the design of a gamified e-learning system for clinical guidelines

Clinical practice guidelines (CPGs) are indispensable in the practice of evidence-based medicine. However, the cost of effective CPG dissemination strategies is prohibitive and not cost-effective. Therefore, scalable strategies using available technology are needed. We describe a formal model-driven approach to design a gamified e-learning system for clinical guidelines. We employ gamification to increase user motivation and engagement in the training of guideline content. Our approach involves the use of models for different aspects of the system, an entity model for the clinical domain, a workflow model for the clinical processes and a game model to manage the training sessions. A game engine instantiates a training session by coupling the workflow and entity models to automatically generate questions based on the data in the model instances. Our approach is flexible and adaptive as it allows for easy updates of the guidelines, integration with different device interfaces and representation of any guideline