402 research outputs found
Entering the age of the new genetics with eyes wide open.
A concerted international effort currently is being made to localize human genes and to identify their role in specific diseases. This will enable physicians to test for a broader range of genetic characteristics and to manipulate human somatic and germ-line cells. With this new age of genetics comes a host of ethical issues and questions
Structural insight into industrially relevant glucoamylases : flexible positions of starch-binding domains
Glucoamylases are one of the most important classes of enzymes in the industrial degradation of starch biomass. They consist of a catalytic domain and a carbohydrate-binding domain (CBM), with the latter being important for the interaction with the polymeric substrate. Whereas the catalytic mechanisms and structures of the individual domains are well known, the spatial arrangement of the domains with respect to each other and its influence on activity are not fully understood. Here, the structures of three industrially used fungal glucoamylases, two of which are full length, have been crystallized and determined. It is shown for the first time that the relative orientation between the CBM and the catalytic domain is flexible, as they can adopt different orientations independently of ligand binding, suggesting a role as an anchor to increase the contact time and the relative concentration of substrate near the active site. The flexibility in the orientations of the two domains presented a considerable challenge for the crystallization of the enzymes
Complex Feeding Tracks of the Sessile Herbivorous Insect Ophiomyia maura as a Function of the Defense against Insect Parasitoids
Because insect herbivores generally suffer from high mortality due to their natural enemies, reducing the risk of being located by natural enemies is of critical importance for them, forcing them to develop a variety of defensive measures. Larvae of leaf-mining insects lead a sedentary life inside a leaf and make conspicuous feeding tracks called mines, exposing themselves to the potential risk of parasitism. We investigated the defense strategy of the linear leafminer Ophiomyia maura Meigen (Diptera: Agromyzidae), by focusing on its mining patterns. We examined whether the leafminer could reduce the risk of being parasitized (1) by making cross structures in the inner area of a leaf to deter parasitoids from tracking the mines due to complex pathways, and (2) by mining along the edge of a leaf to hinder visually searching parasitoids from finding mined leaves due to effective background matching of the mined leaves among intact leaves. We quantified fractal dimension as mine complexity and area of mine in the inner area of the leaf as interior mine density for each sample mine, and analyzed whether these mine traits affected the susceptibility of O. maura to parasitism. Our results have shown that an increase in mine complexity with the development of occupying larvae decreases the probability of being parasitized, while interior mine density has no influence on parasitism. These results suggest that the larval development increases the host defense ability through increasing mine complexity. Thus the feeding pattern of these sessile insects has a defensive function by reducing the risk of parasitism
Observation by an Air-Shower Array in Tibet of the Multi-TeV Cosmic-Ray Anisotropy due to Terrestrial Orbital Motion Around the Sun
We report on the solar diurnal variation of the galactic cosmic-ray intensity
observed by the Tibet III air shower array during the period from 1999 to 2003.
In the higher-energy event samples (12 TeV and 6.2 TeV), the variations are
fairly consistent with the Compton-Getting anisotropy due to the terrestrial
orbital motion around the sun, while the variation in the lower-energy event
sample (4.0 TeV) is inconsistent with this anisotropy. This suggests an
additional anisotropy superposed at the multi-TeV energies, e.g. the solar
modulation effect. This is the highest-precision measurement of the
Compton-Getting anisotropy ever made.Comment: 4 pages, 2 figures, includes .bbl fil
Detection of Multi-TeV Gamma Rays from Markarian 501 during an Unforeseen Flaring State in 1997 with the Tibet Air Shower Array
In 1997, the BL Lac Object Mrk 501 entered a very active phase and was the
brightest source in the sky at TeV energies, showing strong and frequent
flaring. Using the data obtained with a high density air shower array that has
been operating successfully at Yangbajing in Tibet since 1996, we searched for
gamma-ray signals from this source during the period from February through
August in 1997. Our observation detected multi-TeV -ray signals at the
3.7-Sigma level during this period. The most rapid increase of the excess
counts was observed between April 7 and June 16 and the statistical
significance of the excess counts in this period was 4.7-Sigma. Among several
observations of flaring TeV gamma-rays from Mrk 501 in 1997, this is the only
observation using a conventional air shower array. We present the energy
spectrum of gamma-rays which will be worthy to compare with those obtained by
imaging atmospheric Cerenkov telescopes.Comment: 9 pages, 7 figures, To appear in Ap
Observation of Multi-Tev Gamma Rays from the Crab Nebula Using the Tibet Air Shower Array
The Tibet experiment, operating at Yangbajing (4,300 m above sea level), is
the lowest energy air shower array and the new high density array constructed
in 1996 has sensitivity to -ray air showers at energies as low as 3
TeV. With this new array, the Crab Nebula was observed in multi-TeV
-rays and a signal was detected at the 5.5 level. We also
obtained the energy spectrum of -rays in the energy region above 3 TeV
which partially overlaps those observed with imaging atmospheric Cherenkov
telescopes. This is the first observation of -ray signals from point
sources with a conventional air shower array using scintillation detectors.Comment: 9 pages, 4 figures, Accepted for publication in ApJ Letter
The energy spectrum of all-particle cosmic rays around the knee region observed with the Tibet-III air-shower array
We have already reported the first result on the all-particle spectrum around
the knee region based on data from 2000 November to 2001 October observed by
the Tibet-III air-shower array. In this paper, we present an updated result
using data set collected in the period from 2000 November through 2004 October
in a wide range over 3 decades between eV and eV, in which
the position of the knee is clearly seen at around 4 PeV. The spectral index is
-2.68 0.02(stat.) below 1PeV, while it is -3.12 0.01(stat.) above 4
PeV in the case of QGSJET+HD model, and various systematic errors are under
study now.Comment: 12 pages, 7 figures, accepted by Advances in space researc
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