Production system and method for producing fluids from a well

A production system and method for producing fluids from a well are presented. The production system may include a submersible pump and a jet pump. The submersible pump may be arranged within the well. The jet pump may be arranged within the well downstream of the submersible pump. The jet pump may include a power fluid intake configured to receive a power fluid and a produced fluid intake configured to receive a produced fluid. The power fluid intake may be in fluid communication with the submersible pump. The produced fluid intake may be in fluid communication with gas within the well. In an embodiment, the produced fluid intake may be in fluid communication with separated gas within an annulus of the well. Beneficially, the system may allow, among other things, a submersible pump and a jet pump to be used in combination in high gas-liquid-ratio wells without installing a gas vent line.Board of Regents, University of Texas Syste

Production system and method for producing fluids from a well

A production system and method for producing fluids from a well are presented. The production system may include a submersible pump and a jet pump. The submersible pump may be arranged within the well. The jet pump may be arranged within the well downstream of the submersible pump. The jet pump may include a power fluid intake configured to receive a power fluid and a produced fluid intake configured to receive a produced fluid. The power fluid intake may be in fluid communication with the submersible pump. The produced fluid intake may be in fluid communication with gas within the well. In an embodiment, the produced fluid intake may be in fluid communication with separated gas within an annulus of the well. Beneficially, the system may allow, among other things, a submersible pump and a jet pump to be used in combination in high gas-liquid-ratio wells without installing a gas vent line.Board of Regents, University of Texas Syste

Development of Cutaneous Leishmaniasis after Leishmania Skin Test

Thirty-year-old female with a previous history of a cutaneous ulcer suspicious of leishmaniasis 20 years ago presented with a new complaint of a depressed papular lesion 8 × 7 mm in the right lower extremity. The lesion was of 10-day duration. Because early cutaneous leishmaniasis (CL) lesions may have a non-ulcerated appearance, a Leishmania skin test (LST) was performed on the forearm with a strong positive result (38 × 32 mm). After 8 days, the lesion in the leg, which was diagnosed as folliculitis, completely healed. However, a typical CL ulcer (26 × 24 mm) developed at the LST site. Histopathology of the new lesion did not identifiy parasites, but the findings were consistent with a diagnosis of CL. Further analysis identified amastigotes by immunohistochemical stain. Mononuclear cells harvested from the patient were stimulated with Leishmania antigen and showed high levels of production of both tumor necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ): 2,943 pg/mL and 2,313 pg/mL, respectively. After 40 days of treatment with antimony and pentoxifylline, the ulcer resolved. The development of CL at the LST site suggests a strong Th1 immune response, and it is an in vivo documentation of the role of the host immune response in the pathology of CL. It teaches us that LST should be cautiously, if at all, used in patients with self-healing CL ulcers

Morphogenetic and Structural Characteristics of Clones of Elephant Grass Managed under Intermittent Stocking

Morphogenetic studies of the growth dynamics of leaves and tillers of forage grasses have enabled the management strategies for grasses to be defined for various environmental conditions (Euclides et al. 2010). As the development of clones of Pennisetum purpureum is recent (Pereira e Lédo 2008), detailed information on their growth characteristic under pasture is necessary as a reference for the adoption of appropriate management practices. The objective of this study was to assess the morphogenetic and structural characteristics of basal and aerial tillers in pastures of two small-size clones of elephant grass managed under intermittent stocking for six grazing cycles in spring and summer

The role of the biomechanics analyst in swimming training and competition analysis

Swimming analysts aid coaches and athletes in the decision-making by providing evidence-based recommendations. The aim of this narrative review was to report the best practices of swimming analysts that have been supporting high-performance athletes. It also aims to share how swimming analysts can translate applied research into practice. The role of the swimming analyst, as part of a holistic team supporting high-performance athletes, has been expanding and is needed to be distinguished from the job scope of a swimming researcher. As testing can be time-consuming, analysts must decide what to test and when to conduct the evaluation sessions. Swimming analysts engage in the modelling and forecast of the performance, that in short- and mid-term can help set races target-times, and in the long-term provide insights on talent and career development. Races can be analysed by manual, semi-automatic or fully automatic video analysis with single or multi-cameras set-ups. The qualitative and quantitative analyses of the swim strokes, start, turns, and finish are also part of the analyst job scope and associated with race performance goals. Land-based training is another task that can be assigned to analysts and aims to enhance the performance, prevent musculoskeletal injuries and monitor its risk factors.This project was funded by the FCT - Portuguese Foundation for Science and Technology (UIDB/DTP/04045/2020).info:eu-repo/semantics/publishedVersio

Selective enumeration of propionibacteria in Emmental-type cheese using PetrifilmTM Aerobic count plates added to lithium glycerol broth

Propionibacteria derived from dairy products are relevant starter cultures for the production of Swiss and Emmental-type cheeses, and the monitoring of which is mandatory for proper quality control. This study aimed to evaluate an alternative procedure to enumerate propionibacteria, in order to develop a reliable and practical methodology to be employed by dairy industries. 2,3,5-triphenyltetrazolium chloride (TTC) inhibitory activity was tested against five reference strains (CIRM 09, 38, 39, 40 and 116); TTC at 0·0025% (w/v) was not inhibitory, with the exception of one strain (CIRM 116). Subsequently, the four TTC-resistant strains, three commercial starter cultures (PS-1, PB-I, and CHOO) and twelve Emmental-type cheese samples were subjected to propionibacteria enumeration using Lithium Glycerol (LG) agar, and Petrifilm™ Aerobic Count (AC) plates added to LG broth (anaerobic incubation at 30 °C for 7 d). Petrifilm™ AC added to LG broth presented high counts than LG agar (P<0·05) for only two reference strains (CIRM 39, and 40) and for all commercial starter cultures. Cheese sample counts obtained by both procedures did not show significant differences (P<0·05). Significant correlation indexes were observed between the counts recorded by both methods (P<0·05). These results demonstrate the reliability of Petrifilm™ AC plates added to LG broth in enumerating select Propionibacterium spp., despite some limitations observed for specific commercial starter cultures

Integrated approach of hydrogeomorphology and GIS mapping to the evaluation of ground water resources: an example from the hydromineral system of Caldas da Cavaca, NW Portugal.

Understanding the role of geomorphology is essential to accurately assess hydrogeological systems and groundwater resources. Hard-rock watersheds provide a source of valuable water resources. They commonly exhibit complex geological bedrock and morphological features as well as distinctive gradients in rainfall and temperature. A comprehensive evaluation and integrated gro undwater resources study has been carried out for Caldas da Cavaca hydromineral system in NW Portugal, using hydrogeomorphology and GIS mapping techniques. Thematic maps were prepared from satellite imagery, topographical and geological mapping and other hydrogeological field data. These maps were converted to GIS format a nd then integrated using GIS software with the purpose of elaborating a hydrogeomorphological map intended to delineate the infiltration potential areas for the study region. Finally, this study highlights the importance of hydrogeomorphological cartography and groundwater GIS mapping as useful tools to support hydrogeological surveys, as well as for decision-making in the scope of management plans respecting to land and water resources and groundwater sustainability

High variability within Candida albicans transcription factor RLM1: Isolates from vulvovaginal infections show a clear bias toward high molecular weight alleles

Previous studies have correlated the severity of recurrent vulvovaginal Candida infections (VVC) and balanitis in patients from China with the presence of some dominant genotypes at the ORF RLM1. Here we tested VVC vs non-VVC isolates from Portugal, Brazil and Greece and, although the same genotypes were identified in VVC isolates, they were present in only five out of 150 strains. However, this analysis showed that VVC isolates presented a higher percentage of genotypes with similar high molecular weight alleles, in comparison with strains isolated from other biological source

A new tetra-primer ARMS-PCR for genotyping bovine kappa-casein polymorphisms

[EN] Kappa-casein (κ-casein) is one of the most abundant milk proteins. Its main function is to avoid the aggregation of casein micelles, keeping them, and therefore calcium phosphate, in pockets in solution. In bovines, a κ-casein functional polymorphism has been associated with fat, calcium, and protein milk contents and faster curd contraction in cheese production. Quicker curd contraction reduces the loss of milk solids, enhancing cheese yield. This polymorphism induces a double amino acid substitution (Thr136Ile and Ala148Asp). The polymorphism is normally detected by PCR-RFLP, which is a laborious method. An interesting methodological alternative is the tetra-primer amplification refractory mutation system PCR (tetra-primer ARMS-PCR). A tetra-primer ARMS-PCR for the detection of this κ-casein polymorphism has been described. However, specificity was not achieved, probably due to problems with primer design. We developed a new tetra-primer ARMS-PCR for the detection of the κ-casein polymorphism. This new method was validated in a double-blind test, by comparison with the results obtained for 50 Guzerá bulls formerly genotyped by PCR-RFLP. This new method achieved 100% sensitivity and specificity. We conclude that this method is a useful, cost-efficient alternative for the detection of functional κ-casein polymorphismsSIResearch supported by Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG), Conselho Nacional de Pesquisa (CNPq), Pró-Reitoria de Pesquisa de Universidade Federal de Minas gerais (PRPq/UFMG), and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). MRSC, PASF, MAM, and RSV have CNPq fellowships. IRC has a CAPES fellowship. We are grateful to Professor Vânia Maldini Pena and Ms. Ariane Figueiredo Menicucci from Colégio Brasileiro de Criadores do Guzerá, for providing biological sample

Cardiovascular dysfunction in obesity and new diagnostic imaging techniques: the role of noninvasive image methods

Obesity is a major public health problem affecting adults and children in both developed and developing countries. This condition often leads to metabolic syndrome, which increases the risk of cardiovascular disease. A large number of studies have been carried out to understand the pathogenesis of cardiovascular dysfunction in obese patients. Endothelial dysfunction plays a key role in the progression of atherosclerosis and the development of coronary artery disease, hypertension and congestive heart failure. Noninvasive methods in the field of cardiovascular imaging, such as measuring intima-media thickness, flow-mediated dilatation, tissue Doppler, and strain, and strain rate, constitute new tools for the early detection of cardiac and vascular dysfunction. These techniques will certainly enable a better evaluation of initial cardiovascular injury and allow the correct, timely management of obese patients. The present review summarizes the main aspects of cardiovascular dysfunction in obesity and discusses the application of recent noninvasive imaging methods for the early detection of cardiovascular alterations