Formation of polarized contractile interfaces by self-organized Toll-8/Cirl GPCR asymmetry

International audienc

UFMylation of MRE11 is essential for telomere length maintenance and hematopoietic stem cell survival

International audienc

RESULTATS DE LA PROTHESE BIPOLAR* DANS LE TRAITEMENT DES ARTHROPATHIES DEGENERATIVES A COIFFE DETRUITE (A PROPOS DE 46 CAS)

LILLE2-BU Santé-Recherche (593502101) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF

Quantitative proteomic analysis exploring progression of colorectal cancer: Modulation of the serpin family

International audienceColorectal cancer (CRC) remains a major cause of cancer related-death in developed countries. The mortality risk is correlated with the stage of CRC determined at the primary diagnosis and early diagnosis is associated with enhanced survival rate. Currently, only faecal occult blood tests are used to screen for CRC. Consequently, there is an incentive to identify specific markers of CRC. We used quantitative proteomic analysis of serum samples to characterize protein profiles in adenoma, CRC and healthy control samples. We identified 89 distinct proteins modulated between normal, colorectal adenoma and carcinoma patients. This list emphasizes proteins involved in enzyme regulator activities and in particular the serpin family. In serum samples, protein profiles of three members of the serpin family (SERPINA1, SERPINA3 and SERPINC1) were confirmed by ELISA assays. We obtained sensitivity/specificity values of 95%/95% for both SERPINA1 and SERPINC1, and 95%/55% for SERPINA3. This study supports the idea that serum proteins can discriminate adenoma and CRC patients from unaffected patients and reveals a panel of regulated proteins that might be useful for selecting patients for colonoscopy. By evaluating SERPINA1, SERPINA3 and SERPINC1, we highlight the potential role of the serpin family during the development and progression of CRC

Experimental Variability on Modal Characteristics of an In-Situ Pump

Model Validation and Uncertainty Quantification, Volume 3, Orlando, FL, FEB 02-05, 2015Considering that experimental data, usually considered as the reference in test-analysis correlation and model updating purpose, must also be concerned with uncertainty quantification, the experimental variability on modal characteristics of two nominally-identical pumps in industrial environment is observed, via a benchmark between 5 independent teams. Free choice is let to determine the number and the type of sensors, the excitation means, the number of excitation points and the modal identification method. Variabilities relative to the operator and pump specimen are addressed; moreover specific variability relative to the modal identification phase can be quantified using the same experimental test runs. Due to differences on experimental meshes, from 27 to 138 measurement points, a SEREP expansion on a finite element mesh is first performed, allowing the comparison of experimental modal bases. Global variability observed, based on half paired among identified eigenmodes (within 10 % frequency gap and 50 % MAC criteria), shows a 2 % mean frequency gap and 32 % mean damping ratio gap. Unit-to-unit variability, essentially attributed to different actual bolted torque levels at component connections and pipe-to-pump connections, and realization of the frame fixation on concrete, shows 10 % mean frequency gap and 28 % mean damping gap. Specific variability due to modal identification operator, as a component of the global variability, is still significant with a number of identified modes that varies by a factor of 2; the so paired modes show 1 % mean frequency gap and 24 % mean damping ratio gap

Propriétés interfaciales et moussantes de protéines globulaires laitières étuvées

National audienceEn plus de leurs propriétés nutritionnelles, les protéines laitières ont des propriétés fonctionnelles jouant un rôle essentiel dans l'appétence et la qualité organoleptique des aliments. En particulier, les protéines laitières ont des propriétés moussantes résultant de leur aptitude à s’adsorber rapidement à l’interface eau/air, se déployer à l’interface et former un film interfacial résistant mécaniquement. (1) L’auto-assemblage des protéines globulaires à l’interface et les propriétés mécaniques du film sont sensibles à leur structure. Des modifications structurales de faible ampleur, affectant peu la structure tertiaire, peuvent avoir d’importantes conséquences sur leurs propriétés interfaciales et sur leurs propriétés moussantes. (2) Notre objectif est d’étudier le lien entre la structure des protéines du lactosérum, leurs propriétés interfaciales et leurs propriétés moussantes. Ainsi, après avoir procédé à des modifications structurales de faible ampleur de protéines de lactosérum, nous étudions leurs propriétés moussantes. Nous travaillons sur un produit industriel laitier, un isolat de protéines sériques (WPI) et sur de la β-lactoglobuline purifiée (β-Lg), qui en est le principal constituant. Pour modifier la structure des protéines, nous effectuons un étuvage ou traitement thermique à sec. Ce procédé est connu pour améliorer les propriétés moussantes de protéines globulaires d'autres origines. (2) L’effet de différents paramètres d’étuvage sont étudiés : l’activité de l'eau de la poudre, le pH avant déshydratation et la durée de l’étuvage. Les agrégats solubles et insolubles, éventuellement générés, sont éliminés avant d’effectuer des analyses structurales et d’évaluer les propriétés moussantes. En particulier, du fait de la présence résiduelle de lactose dans le WPI, l'impact sur les propriétés moussantes d'une éventuelle lactosylation non enzymatique des protéines dans les conditions de l'étuvage est évalué, grâce à la comparaison avec la β-Lg purifiée. Les liens entre les modifications des propriétés moussantes et celles du comportement des protéines à l'interface eau-air seront discutés

CRB2 controls primary ciliogenesis in association with PAR6α, HOOK2 and RAB8A

Ciliogenesis is an essential process in animal development and physiology and when affected, the origin of severe pathologies called ciliopathies. CRB2 has been recently implicated when mutated in several pathologies such as retina degeneration and a ciliopathy-like syndrome. Here, we have investigated the role of CRB2 in primary ciliogenesis and showed that CRB2 depletion blocks the formation of the ciliary vesicle after activation of the mother centriole, indicating a defect in either transport or anchoring of the ciliary vesicle. CRB2 is accumulated in intracellular vesicles in the vicinity of the centrosome and we showed here that CRB2 forms a complex made of HOOK2 and PAR6α, two proteins important for primary ciliogenesis or centrosome integrity. In addition, CRB2 interacts with RAB8A which over-expression rescued CRB2 knockdown. All together, these findings indicate that CRB2 regulates a membrane transport step essential for the formation of the ciliary vesicle.</jats:p

How foam stability against drainage is affected by conditions of prior whey protein powder storage and dry-heating: A multidimensional experimental approach

International audienceIn the present work, we investigated the effects of powder dry-heating parameters on whey protein foams stability, especially against drainage.To this aim, whey protein isolate solutions were prepared at various pH (3.5, 5.0, 6.5), with or without a prior dialysis step to reduce the lactose content, freeze-dried, adjusted to various levels (0.12, 0.23, 0.52) of powder water activity aw and dry-heated at 70 °C for up to 125 h. Protein solutions were then reconstituted at pH 7.0 and foams prepared by air bubbling.An original approach was followed to study the foam stability against drainage, involving monitoring of the liquid fraction as a function of both height in the foam column and time, and analysing the whole set of time and height liquid fraction profiles using multivariate statistics.The effects of dry-heating parameters were markedly interdependent, resulting in complex effects on foam stability. However, the results suggest that dry-heating at pH 3.5 increased foam stability. Moreover, the aw adjustment step, though consisting in a two-week pre-conditioning at room temperature, also had a significant effect on the foam stability, of the same order of magnitude as dry-heating effects