Rifapentine access in Europe: growing concerns over key tuberculosis treatment component

[No abstract available]Support statement: C. Lange is supported by the German Center of Infection Research (DZIF). All other authors have no funding to declare for this study. Funding information for this article has been deposited with the Crossref Funder Registry

Optimizing multiplex SNP-based data analysis for genotyping of Mycobacterium tuberculosis isolates.

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Dynamics of TB mixed infections through space and time

Abstract de la comunicación oral presentada al Scientific Meeting on Mycobacteria. MycoPORTO 2019 Porto (Portugal), 19-20 de septiembre de 2019Pág. 25 del libro de abstracts que se adjunta. Mixed infections happen when at least two unrelated strains of the same pathogen can be detected in an individual. This has been linked to worse clinical outcomes in tuberculosis infections, as undetected strains presenting different antibiotic resistance profiles can lead to treatment failure. Here, we present a study of the extent of mixed infections in Georgia, a high-burden setting in which up to 11% of new TB cases are MDR/RR-TB. We obtained NGS data from cultures derived from surgery and sputum samples from 20 patients. Combined with a customized bioinformatics pipeline we enhanced the detection of multiple strains as opposed to just using a clinical sputum sample, identifying an unprecedented number of mixed infection cases of up to 40% of the patients analyzed. We also characterized transmission using 358 clinical samples and detected transmission clusters, several of which contained a sample from our surgery patients¿ dataset, allowing us to trace the history of several mixed infections. Our results suggest that the magnitude of mixed infections in highburden settings is likely to be underestimated when only using sputum samples and they can be behind discrepancies between DST and WGS predictions if not properly assessed

Additional file 2: Table S1. of The potential of a multiplex high-throughput molecular assay for early detection of first and second line tuberculosis drug resistance mutations to improve infection control and reduce costs: a decision analytical modeling study

Results of deterministic sensitivity analyses (XLSX 20 kb

Additional file 1: of The potential of a multiplex high-throughput molecular assay for early detection of first and second line tuberculosis drug resistance mutations to improve infection control and reduce costs: a decision analytical modeling study

Expanded Methods and model parameters of treatment outcomes. (DOCX 70 kb