The clinical significance of the arterial ketone body ratio as an early indicator of graft viabilityin human liver transplantation

Arterial ketone body ratio (AKBR) was measured sequentially in 84 liver transplantations (OLTx). These transplantation procedures were classified into 3 groups with respect to graft survival and patient condition at the end of the first month (Group A, the grafts survived longer than 1 month with satisfactory patient condition; Group B, the grafts survived longer than 1 month but the patients were ICU-bound; Group C, the grafts were lost and the patients died or underwent re-OLTx). In Group A, the AKBR was elevated to above 1.0 by the second postoperative day. In Group B, the AKBR was elevated to above 0.7 but stayed below 1.0 during this period. In Group C, the AKBR remained below 0.7 longer than 2 days after operation. Although conventional liver function tests showed significant increases in Groups B and C as compared with Group A, they were less specific in predicting ultimate graft survival. © 1991 by Williams & Wilkins

Electrochemical reaction engineering of polymer electrolyte fuel cell

Although fuel cells can be considered as a type of reactor, methods of kinetic analysis and reactor modeling from the viewpoint of chemical reaction engineering have not yet been established. The rate of an electrochemical reaction is a function of concentration, temperature, and interfacial potential difference (or electromotive force). This study examined the cathode reaction in a polymer electrolyte fuel cell, in which oxygen and protons react over platinum in the catalyst layer (CL). The effects of the oxygen partial pressure and the cathode electromotive force on the reaction rate were assessed. Resistance to proton transport increases the electromotive force and reducing the reaction rate. It was established that the effectiveness factor of the cathode CL is determined by competition between the reaction and mass transport of oxygen and protons. Two dimensionless moduli that govern the cathode behavior are proposed as a means of depicting the processes in the cell

M2 Macrophages Activate WNT Signaling Pathway in Epithelial Cells: Relevance in Ulcerative Colitis

Macrophages, which exhibit great plasticity, are important components of the inflamed tissue and constitute an essential element of regenerative responses. Epithelial Wnt signalling is involved in mechanisms of proliferation and differentiation and expression of Wnt ligands by macrophages has been reported. We aim to determine whether the macrophage phenotype determines the expression of Wnt ligands, the influence of the macrophage phenotype in epithelial activation of Wnt signalling and the relevance of this pathway in ulcerative colitis. Human monocyte-derived macrophages and U937-derived macrophages were polarized towards M1 or M2 phenotypes and the expression of Wnt1 and Wnt3a was analyzed by qPCR. The effects of macrophages and the role of Wnt1 were analyzed on the expression of β-catenin, Tcf-4, c-Myc and markers of cell differentiation in a co-culture system with Caco-2 cells. Immunohistochemical staining of CD68, CD206, CD86, Wnt1, β-catenin and c-Myc were evaluated in the damaged and non-damaged mucosa of patients with UC. We also determined the mRNA expression of Lgr5 and c-Myc by qPCR and protein levels of β-catenin by western blot. Results show that M2, and no M1, activated the Wnt signaling pathway in co-culture epithelial cells through Wnt1 which impaired enterocyte differentiation. A significant increase in the number of CD206+ macrophages was observed in the damaged mucosa of chronic vs newly diagnosed patients. CD206 immunostaining co-localized with Wnt1 in the mucosa and these cells were associated with activation of canonical Wnt signalling pathway in epithelial cells and diminution of alkaline phosphatase activity. Our results show that M2 macrophages, and not M1, activate Wnt signalling pathways and decrease enterocyte differentiation in co-cultured epithelial cells. In the mucosa of UC patients, M2 macrophages increase with chronicity and are associated with activation of epithelial Wnt signalling and diminution in enterocyte differentiation

Biliary atresia

Biliary atresia (BA) is a rare disease characterised by a biliary obstruction of unknown origin that presents in the neonatal period. It is the most frequent surgical cause of cholestatic jaundice in this age group. BA occurs in approximately 1/18,000 live births in Western Europe. In the world, the reported incidence varies from 5/100,000 to 32/100,000 live births, and is highest in Asia and the Pacific region. Females are affected slightly more often than males. The common histopathological picture is one of inflammatory damage to the intra- and extrahepatic bile ducts with sclerosis and narrowing or even obliteration of the biliary tree. Untreated, this condition leads to cirrhosis and death within the first years of life. BA is not known to be a hereditary condition. No primary medical treatment is relevant for the management of BA. Once BA suspected, surgical intervention (Kasai portoenterostomy) should be performed as soon as possible as operations performed early in life is more likely to be successful. Liver transplantation may be needed later if the Kasai operation fails to restore the biliary flow or if cirrhotic complications occur. At present, approximately 90% of BA patients survive and the majority have normal quality of life

EVALUATION OF CORRECTION METHODS OF CHROMATIC ABERRATION IN DIGITAL CAMERA IMAGES

This paper reports an experiment conducted to evaluate correction methods of chromatic aberrations in images acquired by a nonmetric digital camera. The chromatic aberration correction methods evaluated in the experiment are classified into two kinds. One is the method to correct image coordinates by using camera calibration results of color-separated images. The other is the method based on the assumption that the magnitude of chromatic aberrations can be expressed by a function of a radial distance from the center of an image frame. The former is classified further into five types according to the difference of orientation parameters common to all colors. The latter is classified further into three types according to the order of the correction function. We adopt a linear function, a quadratic function and a cubic function of the radial distance as a correction function. We utilize a set of 16 convergent images shooting a white sheet with 10 by 10 black filled circles to carry out camera calibration and estimate unknown coefficients in the correction function by means of least squares adjustment. We evaluate the chromatic aberration correction methods by using a normal image shooting a white sheet with 14 by 10 black filled circles. From the experiment results, we conclude that the method based on the assumption that the magnitude of chromatic aberrations can be expressed by a cubic function of the radial distance is the best method of the evaluated methods, and would be able to correct chromatic aberrations satisfactorily enough in many cases