Chronomodulated rupturable pulsatile drug delivery of theophylline: preparation and in vitro characterization

The aim of the present study was to develop and evaluate chronomodulated rupturable pulsatile drug delivery of theophylline for the treatment of nocturnal asthma. Core tablets containing theophylline were coated with two consecutive layers, an inner swelling layer and an outer rupturable coating layer. The effect of formulation variables such as molecular weight of outer rupturable coating layer, type and amount of swelling layer, weight gain of coating film and influence of paddle speed on drug release were investigated. The drug release from the pulsatile tablets exhibited an initial lag period, followed by a stage of rapid drug release. The optimal level of swelling layer (crospovidone) to achieve a fast and complete release was 20 % w/w. The results indicated that lag time was prolonged with the increased weight gain of coating film. Also no significant difference in the drug release was observed for different rotational speeds. In accordance with the chronomodulated therapy of asthma, the lag time criterion of 5 hours was satisfied by formulation having 11 % weight gain of outer rupturable layer.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Correlation between 6-minute walk distance and spirometry parameters in stable chronic obstructive pulmonary disease patients

Background: Six minute walk test (6MWT) is a sub-maximal exercise test, used as a clinical indicator of the functional capacity, in patients with cardiopulmonary diseases. It is simple, objective and reproducible test. The present study was designed to assess correlation of six minute walk test with spirometry parameters, in patients with chronic obstructive pulmonary disease.Methods: In this cross sectional study, fifty patients diagnosed with chronic obstructive pulmonary disease (GOLD criteria) coming to tertiary center were recruited according to inclusion and exclusion criteria. All patients underwent spirometric measurement. Spirometric indices including FEV1, FVC, FEV1/FVC and MVV were tested using computerized spirometer. 6MWT was performed following American Thoracic Society (ATS) guidelines. Percent (%) predicted 6MWD was calculated. Correlation between spirometry and 6MWT was assessed.Results: It was found that correlation between 6MWT and spirometry is statistically significant. There is significant strong positive correlation between percent predicted 6MWD and FEV1 (r=0.850 and p= <0.001), whereas there is significant moderate correlation between percent predicted 6MWD and FVC (r=0.554 and p= <0.001), FEV1/FVC (r=0.509 and p= <0.001) and MVV (r=0.615 and p= <0.001).Conclusions: In chronic obstructive pulmonary disease, percent predicted 6mwd significantly correlated with the spirometry parameters (FEV1, FVC, FEV1/FVC, and MVV). 6MWD decreases as there is decline in the pulmonary function. 6MWT can be a useful replacement of spirometry in assessment of severity of COPD

Thermostable phytase in feed and fuel industries

Phytase with wide ranging biochemical properties has long been utilized in a multitude of industries, even so, thermostability plays a crucial factor in choosing the right phytase in a few of the sectors. Mesophilic phytases are not considered to be a viable option in the feed industry owing to its limited stability in the required feed processing temperature. In the recent past, inclusion of thermostable phytase in fuel ethanol production from starch based raw material has been demonstrated with economic benefits. Therefore, considerable emphasis has been placed on using complementary approaches such as mining of extremophilic microbial wealth, encapsulation and using enzyme engineering for obtaining stable phytase variants. This article means to give an insight on role of thermostable phytases in feed and fuel industries and methods for its development, highlighting molecular determinants of thermostability

INTEGRATED APPROACH OF SCHEDULING A FLEXIBLE JOB SHOP USING ENHANCED FIREFLY AND HYBRID FLOWER POLLINATION ALGORITHMS

Manufacturing industries are undergoing tremendous transformation due to Industry 4.0. Flexibility, consumer demands, product customization, high product quality, and reduced delivery times are mandatory for the survival of a manufacturing plant, for which scheduling plays a major role. A job shop problem modified with flexibility is called flexible job shop scheduling. It is an integral part of smart manufacturing. This study aims to optimize scheduling using an integrated approach, where assigning machines and their routing are concurrently performed. Two hybrid methods have been proposed: 1) The Hybrid Adaptive Firefly Algorithm (HAdFA) and 2) Hybrid Flower Pollination Algorithm (HFPA). To address the premature convergence problem inherent in the classic firefly algorithm, the proposed HAdFA employs two novel adaptive strategies: employing an adaptive randomization parameter (α), which dynamically modifies at each step, and Gray relational analysis updates firefly at each step, thereby maintaining a balance between diversification and intensification. HFPA is inspired by the pollination strategy of flowers. Additionally, both HAdFA and HFPA are incorporated with a local search technique of enhanced simulated annealing to accelerate the algorithm and prevent local optima entrapment. Tests on standard benchmark cases have been performed to demonstrate the proposed algorithm’s efficacy. The proposed HAdFA surpasses the performance of the HFPA and other metaheuristics found in the literature. A case study was conducted to further authenticate the efficiency of our algorithm. Our algorithm significantly improves convergence speed and enables the exploration of a large number of rich optimal solutions.

Pulsatile drug delivery for the treatment of nocturnal asthma: a chronopharmaceutical approach

The objective of the present study was to develop and evaluate a pulsatile system of theophylline based on chronopharmaceutical considerations. The basic design consists of an impermeable capsule body, which contains the drug and is closed by an erodible tablet plug. The body portion of the hard gelatin capsules was cross-linked by the combined effect of formaldehyde and heat treatment. The formulation variables such as type of plug material, capsule content, different plug composition, plug weight and plug hardness was investigated to characterize the lag time (t10).The results indicated that drug release from the pulsatile capsule exhibited an initial lag period, followed by a stage of rapid drug release. For the complete and rapid drug release from the capsule body, 15% of effervescent agent had to be included in the capsule content. The lag time criterion of 5 hrs was satisfied by the tablet plug containing 16%HPMC K100LV. A good correlation was observed between erodible tablet weight and lag time.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Chronomodulated rupturable pulsatile drug delivery of theophylline: preparation and in vitro characterization

The aim of the present study was to develop and evaluate chronomodulated rupturable pulsatile drug delivery of theophylline for the treatment of nocturnal asthma. Core tablets containing theophylline were coated with two consecutive layers, an inner swelling layer and an outer rupturable coating layer. The effect of formulation variables such as molecular weight of outer rupturable coating layer, type and amount of swelling layer, weight gain of coating film and influence of paddle speed on drug release were investigated. The drug release from the pulsatile tablets exhibited an initial lag period, followed by a stage of rapid drug release. The optimal level of swelling layer (crospovidone) to achieve a fast and complete release was 20 % w/w. The results indicated that lag time was prolonged with the increased weight gain of coating film. Also no significant difference in the drug release was observed for different rotational speeds. In accordance with the chronomodulated therapy of asthma, the lag time criterion of 5 hours was satisfied by formulation having 11 % weight gain of outer rupturable layer.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Role of male behavior in cervical carcinogenesis among women with one lifetime sexual partner

Background: The role of male behavior in the genesis of cervical cancer was examined. In India, where the incidence of cervical cancer is among the highest in the world, promiscuity among women is virtually unknown. In this study, the authors investigated the role of male behavior in cervical carcinogenesis among Indian women who had one lifetime sexual partner. Methods: A case-control study was used. Results. Premarital sexual relationships (relative risk [RR], 1.9; confidence interval, 1.2-3.2) and extramarital sexual relationships (RR, 2.7; confidence interval, 1.5-4.9) of husbands were risk factors. When husbands had sexual relationships both before and during the marriage, their wives' risk of getting cervical cancer increased by 6.9 (CI, 2.3-20.7). Risk also increased with husbands having three or more extramarital sexual partners (RR, 3.05; CI, 1.25-12.6). Sexual contact with prostitutes before or after marriage, however, did not increase the risk. History of sexually transmitted disease before marriage (RR, 2.9) or after marriage (RR, 5.9) was an important risk factor, which persisted after controlling for other factors. Sexual abstinence for 40 or more days after a wife's giving birth or having an abortion provided protection. Sex with uncircumcised men or men circumcised after age 1 year increased the risk of cervical cancer (RR, 4.1). Bidi smoking (bidi is a cheap smoking stick of 4-8 cm, consisting of a rolled piece of dried temburni leaf [Diospyres melanoxylon] containing 0.15-0.25 g of coarsely ground tobacco) for more than 20 years was a significant risk factor (RR = 2.4), whereas cigarette smoking was not a risk factor. Conclusions: Male sexual partners play a role in cervical carcinogenesis

Identificação de pacientes portadores do fator de infertilidade masculina através do cálculo de um novo escore de qualidade de sêmen e pela medida de espécies reativas de oxigênio

PURPOSE: To determine whether patients with male factor infertility can be accurately identified by calculating a novel semen quality score and measuring levels of reactive oxygen species during routine infertility screening. METHODS: Semen samples from 133 patients and 91 healthy donors were evaluated with manual and computer-assisted semen analysis. A principal component analysis model was employed to calculate a semen quality score. In brief, this score was calculated by base 10 logarithms multiplied by varying weights given to 9 sperm parameters. Reactive oxygen species levels were measured using chemiluminescence assay. RESULTS: The semen quality score had a sensitivity of 80.45% and accuracy of 77% at a cutoff of 93.1 in identifying patients with male factor infertility. The area under the receiver operating characteristic curves for the semen quality score was 84.28% (95% CI: 65.22%-100%). Reactive oxygen species levels [log10 (reactive oxygen species +1)] were significantly higher in male factor infertility patients. Reactive oxygen species had a sensitivity of 83.47% and specificity of 60.52% with an accuracy of 75% at a cutoff of 1.25 in identifying male factor infertility patients. The area under the receiver operating characteristic curve for reactive oxygen species levels was 78.92% (95% CI: 72.60%-85.23%). semen quality scores were significantly and negatively correlated with reactive oxygen species levels in the donors and the male factor infertility patients. CONCLUSIONS: The semen quality score and reactive oxygen species levels in semen samples appear to be strongly associated with male factor infertility. Because both of these parameters are more sensitive than individual sperm parameters in identifying male factor infertility, they should be included in routine infertility screening.OBJETIVO: Determinar se pacientes portadores do fator de infertilidade masculina podem ser precisamente identificados através do cálculo de um novo escore de qualidade de sêmen e pela medida de espécies reativas de oxigênio durante uma avaliação rotineira de infertilidade. MÉTODOS: Amostras de sêmen de 133 pacientes e de 91 doadores saudáveis foram avaliadas através de análise manual e computadorizada de sêmen. Um modelo de análise do componente principal foi empregado para calcular o escore de qualidade de sêmen, utilizando logaritmos base 10, multiplicados por ponderações variáveis de 9 parâmetros espermáticos. Os níveis de espécies reativas de oxigênio foram medidos através de testes de quimiluminescência. RESULTADOS: O escore de qualidade de sêmen apresentou sensibilidade de 80.45% e precisão de 77% para um "cutoff" de 93.1 na identificação do fator de infertilidade masculina. A área sob a curva "receiver operating characteristic" para o escore de qualidade de sêmen foi de 84.28% (95% intervalo de confiança: 65.22%-100%). Os níveis de espécies reativas de oxigênio [log10 (espécies reativas de oxigênio +1)] foram siginificativamente mais elevados nos pacientes portadores de fator de infertilidade masculina. A medica de espécies reativas de oxigênio apresentou sensibilidade de 83.47% e especificidade de 60.52% com uma precisão (definida como pacientes portadores do fator de infertilidade masculina com diagnóstico positivo e doadores corretamente excluídos) de 75% para um "cutoff" de 1.25 na identificação de pacientes portadores do fator de infertilidade masculina. A área sob a curva "receiver operating characteristic" para níveis de espécies reativas de oxigênio foi de 78.92% (95% intervalo de confiança: 72.60%-85.23%). Os escores de qualidade de sêmen correlacionaram negativamente com os níveis de espécies reativas de oxigênio tanto nos doadores e nos pacientes portadores do fator de infertilidade masculina. CONCLUSÕES: O escore de qualidade de sêmen e os níveis espécies reativas de oxigênio nas amostras de sêmen parecem associar-se fortemente com o fator de infertilidade masculina. Na medida em que os dois parâmetros mostraram-se mais sensíveis que parâmetros espermáticos individuais na identificação do fator de infertilidade masculina, deveriam ser incluídos na avaliação rotineira de infertilidade

Remote preconditioning by aortic constriction: affords cardioprotection as classical or other remote ischemic preconditioning? Role of iNOS

Dose remote preconditioning by aortic constriction (RPAC) affords cardioprotection similar to classical or other remote ischemic preconditioning stimulus? Moreover study was also designed to investigate role of inducible nitric oxide synthase in remote preconditioning by aortic constriction. There are sufficient evidences that &#x22;ischemic preconditioning&#x22; has surgical applications and afford clinically relevant cardioprotection. Transient occlusion of circumflex artery, renal artery, limb artery or mesenteric artery preconditions the myocardium against ischemia reperfusion injury in case of ischemic heart disease leading to myocardial infraction. Here abdominal aorta was selected to produce RPAC. Four episodes of Ischemia-reperfusion of 5 min each to abdominal aorta produced RPAC by assessment of infract size, LDH and CK. These studies suggest RPAC produced acute (FWOP) and delayed (SWOP) cardioprotective effect. RPAC demonstrated a significant decrease in Ischemia-reperfusion induced release of LDH, CK and extent of myocardial infract size. L-NAME (10 mg/Kg i.v.), Aminoguanidine (150 mg/Kg s.c.), Aminoguanidine (300 mg/Kg s.c.), S-methyl isothiourea (3 mg/Kg i.v.), 1400W (1 mg/Kg i.v.) administered 10 min. before global ischemia reperfusion produced no marked effect. Aminoguanidine (150 mg/Kg s.c.), Aminoguanidine (300 mg/Kg s.c.), S-methyl isothiourea (3 mg/Kg i.v.), 1400W (1 mg/Kg i.v.) pretreatment after RPAC produced no significant effect on acute RPAC induced decrease in LDH, CK and infract size, whereas L-NAME (10 mg/Kg i.v.) increased RPAC induced decrease in LDH, CK and infract size. Most interesting observation is in delayed RPAC, where all NOS inhibitors pretreatment attenuate RPAC induced decrease in LDH, CK and infract size. In conclusions, &#x22;Remote preconditioning by aortic constriction&#x22; (RPAC) affords cardioprotection similar to classical or other remote ischemic preconditioning stimulus. Moreover, late or delayed phase of RPAC has been mediated by inducible nitric oxide synthase (iNOS) whereas it has not involved in acute RPAC

Thoracic spine stab injury with pneumocephalus and pneumorrhachiasis: A unique case report and review of literature

Trauma to skull base or sinus is the most common cause of pneumocephalus. There are only few published reports of traumatic pneumocephalus secondary to penetrating injury of upper spine, epidural anaesthesia and lumber puncture. Till date only one case of pneumocephalus and pneumorrhachiasis after thoracic spine stab injury is reported in available English literature that too associated without any neurological deficit. To our knowledge, this is the first reported case of pneumocephalus and pneumorrhachiasis after thoracic spine stab injury with asymmetrical, incomplete spinal cord injury leading to neurological deficit in the patient. The management protocol of such injuries needs to be individualised for better outcome