IUSM-Purdue TREAT-AD Center Target Enabling Report LYN, Src family tyrosine-protein kinase: Protein Constructs, Cryo-EM, and Biochemical Assay

A Target Enabling Report detailing Protein Constructs, Cryo-EM and Biochemical assay for LY

IUSM-Purdue TREAT-AD Center Target Enabling Report Proline-rich Tyrosine Kinase 2 beta (PTK2β): Protein Constructs, Cryo-EM, Biophysical Assay, and Biochemical Assay

A Target Enabling report detailing the Protein Construct, Cryo-EM, Biophysical Assay and Biochemical Assay work around PTK2

IUSM-Purdue TREAT-AD Center Target Enabling Report Proline-rich Tyrosine Kinase 2 beta (PTK2β): Protein Constructs, Cryo-EM, Biophysical Assay, and Biochemical Assay

A Target Enabling report detailing the Protein Construct, Cryo-EM, Biophysical Assay and Biochemical Assay work around PTK2

IUSM-Purdue TREAT-AD Center Target Enabling Report LYN, Src family tyrosine-protein kinase: Protein Constructs, Cryo-EM, and Biochemical Assay

A Target Enabling Report detailing Protein Constructs, Cryo-EM and Biochemical assay for LY

Alcoholic Extract of Eclipta alba

As per WHO estimates, 80% of people around the world use medicinal plants for the cure and prevention of various diseases including cancer owing to their easy availability and cost effectiveness. Eclipta alba has long been used in Ayurveda to treat liver diseases, eye ailments, and hair related disorders. The promising medicinal value of E. alba prompted us to study the antioxidant, nontoxic, and anticancer potential of its alcoholic extract. In the current study, we evaluated the in vitro cytotoxic and antioxidant effect of the alcoholic extract of Eclipta alba (AEEA) in multiple cancer cell lines along with control. We have also evaluated its effect on different in vivo toxicity parameters. Here, we found that AEEA was found to be most active in most of the cancer cell lines but it significantly induced apoptosis in human breast cancer cell lines by disrupting mitochondrial membrane potential and DNA damage. Moreover, AEEA treatment inhibited migration in both MCF 7 and MDA-MB-231 cells in a dose dependent manner. Further, AEEA possesses robust in vitro antioxidant activity along with high total phenolic and flavonoid contents. In summary, our results indicate that Eclipta alba has enormous potential in complementary and alternative medicine for the treatment of cancer

Saraca indica

Medicinal plants are used as a complementary and alternative medicine in treatment of various diseases including cancer worldwide, because of their ease of accessibility and cost effectiveness. Multicomposed mixture of compounds present in a plant extract has synergistic activity, increases the therapeutic potential many folds, compensates toxicity, and increases bioavailability. Saraca indica (family Caesalpiniaceae) is one of the most ancient sacred plants with medicinal properties, exhibiting a number of pharmacological effects. Antioxidant, antibreast cancer activity and toxicological evaluation of Saraca indica bark extract (SIE) were carried out in the present study. The results of the study indicated that this herbal preparation has antioxidant and antibreast cancer activity. Toxicological studies suggest that SIE is safer to use and may have a potential to be used as complementary and alternative medicine for breast cancer therapy

Pharmacognostical evaluation of Indian folk-traditional plants<em> Coelogyne cristata and Pholidota articulata</em> used for healing fractures

622-627Coelogyne cristata Lindley (CC) and Pholidota articulata Lindley (PA) (Fam. Orchidaceae), locally known as 'hadjojen' (bone jointer) are the most effective traditional remedies for healing fractures in Uttarakhand Himalaya, India. Recent pharmacological investigations of crude extracts and isolated compounds from these plant species revealed rapid fracture healing properties and osteogenic potential. This paper provides macro and microscopic characteristic features, physicochemical properties and HPTLC profiles of both the species. Microscopic studies of leaf, pseudobulb and powdered materials showed collateral vascular bundles containing large number of mucilage cells, parenchymatous cells with pitted banded lignified or beaded with mesh-like network, septate and aseptate fibres, rhomboidal crystals of calcium oxalate and pitted parenchyma. Comparative HPTLC profile showed blue and pink florescent band at different Rf values with the distinct characteristic bands at Rf 0.31, 0.47 and 0.62 corresponding to the analytical marker compounds: ursolic acid, -sitosterol and lupeol respectively in both CC and PA. Findings of this study can be used a standardized pharmacognostical markers of CC and PA for identification and authentication of their genuine herbal drug formulations as quality control markers

Automatic Classification and Color Changing of Saree Components Using Deep Learning Techniques

Sarees are integral to Indian culture and serve as daily attire for most women on the subcontinent. Despite their popularity, there exists a gap in research regarding the automatic segmentation of sarees and the independent color modification of distinct components. Existing methods rely on labor-intensive manual adjustments through commercial applications, impeding productivity and resulting in avoidable expenses. This paper presents a tool that smartly coordinates different deep-learning techniques to modify the color patterns found on different parts of a saree. MODNet is applied for background removal and custom-trained Mask R-CNN models are utilized to precisely segment the saree body and border. The subsequent application of a color-changing algorithm in the HSV color space facilitates independent color modification for the saree border and body. The methodology proposed in this paper can be extended to any kind of clothing such as shirts, trousers, kurtas, kimonos, etc. An accuracy of 93.01&#x0025; was achieved for the saree border segmentation, and an accuracy of 89.23&#x0025; was achieved for the saree body segmentation when tested on a set of 50 test images

LC-MS/MS method for the simultaneous quantification of luteolin, wedelolactone and apigenin in mice plasma using hansen solubility parameters for liquid-liquid extraction: application to pharmacokinetics of Eclipta alba chloroform fraction

Eclipta alba (Bhringraj) in ayurveda has been widely used as a traditional medicine for its multi-therapeutic properties for ages. Luteolin (LTL), wedelolactone (WDL) and apigenin (APG) are the three main bioactive phytochemicals present in Eclipta alba extract. However there was a lack of sensitive bioanalytical method for the pharmacokinetics of these free compounds in plasma which majorly contributes for their activities after oral administration of Eclipta alba. The present study aims to develop a sensitive, rapid and reliable liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the simultaneous estimation of mice plasma concentrations of LTL, WDL and APG using quercetin as an internal standard for the pharmacokinetic analysis. Analytes were separated on Phenomenex Luna C18 (150 × 4.6 mm, 3.0 μm) column with mobile phase containing methanol: acetonitrile (90: 10, v/v) and 0.1% formic acid in 10 mM ammonium formate buffer in the ratio of 70: 30 (v/v) in isocratic mode. Liquid-liquid extraction was optimized using Hansen solubility parameters and diethyl ether finalized as an extraction solvent for the recovery ranging from 61 to 76% for all analytes in mice plasma. The validated method has an accuracy and precision over the linearity range of 0.1–200 ng/mL with a correlation coefficient (r2) of ≥0.997. The intra and inter-day assay accuracy was between 98.17 and 107% and 95.83–107.89% respectively and the intra and inter day assay precision ranged from 0.37–6.05% and 1.85–10.76%, respectively for all the analytes. This validated method can be used for future clinical investigation studies of Eclipta alba extracts