Raja Bandung Banana (Musa ParadisiacaL.cv Raja Bandung) Prevents Increased Systolic Blood Pressure in Rats Given Acute Stress Test

The relationships between stress and hypertension have been evaluated. Heightened blood pressure (BP) reactions to acute stress have been implicated in cardiovascular disease\u27s development. Consumption of fruit or vegetables lowering BP. This study aimed to evaluate the effect of Raja Bandung Banana (Musa paradisiacaL. cv Raja Bandung) on blood pressure after acute restraint stress and forced swim test.Twenty male Sprague dawley rats were divided into 4 groups (A,B,C,D) and adapted for 3 days. At 4th day, groups were administered 2 g/200gBw AIN-93M, then exposed to acute restraint (1hour), except group A. After restraint, A&B received water, C received diazepam 0,5mg/kgBW, and D received banana 2,52 g/200gBW. One hour later, the forced swim test was carried out (45 minutes). Blood pressure was measured 1 hour after swim. Result showed,mean of blood pressure significantly increased after treatment (p<0,01) all groups. But, the increasing of blood pressure in C and D group was lower than unrestraint (A) and control (B) group. There was significant difference of blood pressure between control (A&B) and intervention group (C&D), but no significant difference between C and D. Thisresults indicate that Raja Bandung Banana can prevent increased blood pressure on acute stress condition as effective as diazepam

Emping Garut (Maranta Arundinacea Linn) sebagai Makanan Ringan dan Kadar Glukosa Darah, Angiotensin II Plasma Serta Tekanan Darah pada Penderita Diabetes Mellitus Tipe 2 (DMT2)

Consumption of crispy arrowroot (Maranta arundinacea Linn), blood glucose and angiotensin II level, and blood pressure in type 2 diabetesBackground: The mortality of DM caused by cardiovascular complication is about 75% can be reduce by using food with lowglycemic index. The level of fiber consumption on DM sufferers is low (12.08±3.80 gr/day). Crispy arrowroot has 14 glycemic index. The purpose of this study is to know the influence of crispy arrowroot giving to the fasting plasma glucose, angiotensin II rate and blood pressure and also to study the correlation between angiotensin II and blood pressure before and after the giving of crispy arrowroot.Method: This research used quasi-experimental design of pre and post test one group design, the subjects were 14 DMT2 female sufferers (35 to 60 years old), the length of suffering from DM was 1 year, and used insulin therapy. The giving of crispy arrowroot was 20 gr/day for four weeks. The determination of fasting blood glucose rate used colorimetry, angiotensin II rate used ELISA sandwich method and blood pressure used spignomanometer. The data analysis used paired t test and regression test.Results: Consumption of crispy arrowroot does not increase fasting blood sugar (p=0.551), or decrease angiotensin II (p=0.550), and systolic-diastolic blood pressure (psystolic=0.518; pdiastolic=0.216). Conclusion: Crispy arrowroot of 20 g/day for 4 weeks does not give any benefit to DM type 2 patients

Analisis Dan Perancangan E-commerce Pd. Garuda Jaya

Tujuan penelitian ini adalah menganalisis dan merancang sebuah sistem e-commerce pada PD.Garuda Jaya untuk membantu proses penjualan yang dapat melakukan pemesanan secara online danmemberikan informasi yang up to date kepada pelanggan. Saat ini, pemilik PD. Garuda Jaya menggunakanyellow pages untuk promosi dan juga didalam memesan barang, pelanggan langsung datang ke toko danmelalui telepon. Metode penelitian yang digunakan adalah metode analisa, dan metode perancangan. Metodeanalisa dilakukan dengan studi pustaka, pengumpulan data (observasi), dan Market Opportunity analysis.Sedangkan metode perancangan dilakukan dengan business model, customer interface, market communicationand branding, implementation, dan metrics. Hasil yang dicapai adalah bahwa PD. Garuda Jaya membutuhkan sistem penjualan yang berbasis webatau e-commerce, sehingga mempermudah pemesanan oleh pelanggan. Dengan adanya sistem ini diharapkantarget pemasaran dapat berkembang. Kesimpulan yang dapat diambil adalah internet merupakan sebuah mediayang dapat membantu dan mendukung dalam pengembangan bisnis

Out-of-Sequence Signal 3 Paralyzes Primary CD4+ T-Cell-Dependent Immunity

SummaryPrimary T cell activation involves the integration of three distinct signals delivered in sequence: (1) antigen recognition, (2) costimulation, and (3) cytokine-mediated differentiation and expansion. Strong immunostimulatory events such as immunotherapy or infection induce profound cytokine release causing “bystander” T cell activation, thereby increasing the potential for autoreactivity and need for control. We show that during strong stimulation, a profound suppression of primary CD4+ T-cell-mediated immune responses ensued and was observed across preclinical models and patients undergoing high-dose interleukin-2 (IL-2) therapy. This suppression targeted naive CD4+ but not CD8+ T cells and was mediated through transient suppressor of cytokine signaling-3 (SOCS3) inhibition of the STAT5b transcription factor signaling pathway. These events resulted in complete paralysis of primary CD4+ T cell activation, affecting memory generation and induction of autoimmunity as well as impaired viral clearance. These data highlight the critical regulation of naive CD4+ T cells during inflammatory conditions

Mapping ergonomics application to improve SMEs working condition in industrially developing countries: a critical review

In industrially developing countries (IDC), small and medium enterprises (SMEs) account for the highest proprotion of employment. Unfortunately, the working conditions in SMEs are often very poor and expose employees to a potentially wide range of health and safety risks. This paper presents a comprehensive review of 161 articles related to ergonomics application in SMEs, using Indonesia as a case study. The aim of this paper is to investigate the extent of ergonomics application and identify areas that can be improved to promote effective ergonomics for SMEs in IDC. The most urgent issue found is the need for adopting participatory approach in contrast to the commonly implemented top-down approach. Some good practices in ergonomics application were also revealed from the review, e.g. a multidisciplinary approach, unsophisticated and low-cost solutions, and recognising the importance of productivity. The review also found that more work is still required to achieve appropriate cross-cultural adaptation of ergonomics application. Practitioner Summary: Despite continuous efforts in addressing ergonomics issues in SMEs of IDC, workers are still exposed to poor work conditions. We reviewed factual-based evidence of current ergonomics application to inform future strategies of ergonomics in IDC, using Indonesia as a case study

CALOCUBE: An approach to high-granularity and homogenous calorimetry for space based detectors

Future space experiments dedicated to the observation of high-energy gamma and cosmic rays will increasingly rely on a highly performing calorimetry apparatus, and their physics performance will be primarily determined by the geometrical dimensions and the energy resolution of the calorimeter deployed. Thus it is extremely important to optimize its geometrical acceptance, the granularity, and its absorption depth for the measurement of the particle energy with respect to the total mass of the apparatus which is the most important constraint for a space launch. The proposed design tries to satisfy these criteria while staying within a total mass budget of about 1.6 tons. Calocube is a homogeneous calorimeter instrumented with Cesium iodide (CsI) crystals, whose geometry is cubic and isotropic, so as to detect particles arriving from every direction in space, thus maximizing the acceptance; granularity is obtained by filling the cubic volume with small cubic CsI crystals. The total radiation length in any direction is more than adequate for optimal electromagnetic particle identification and energy measurement, whilst the interaction length is at least sufficient to allow a precise reconstruction of hadronic showers. Optimal values for the size of the crystals and spacing among them have been studied. The design forms the basis of a three-year R&D activity which has been approved and financed by INFN. An overall description of the system, as well as results from preliminary tests on particle beams will be described

Impact of infection on proteome-wide glycosylation revealed by distinct signatures for bacterial and viral pathogens

Mechanisms of infection and pathogenesis have predominantly been studied based on differential gene or protein expression. Less is known about posttranslational modifications, which are essential for protein functional diversity. We applied an innovative glycoproteomics method to study the systemic proteome-wide glycosylation in response to infection. The protein site-specific glycosylation was characterized in plasma derived from well-defined controls and patients. We found 3862 unique features, of which we identified 463 distinct intact glycopeptides, that could be mapped to more than 30 different proteins. Statistical analyses were used to derive a glycopeptide signature that enabled significant differentiation between patients with a bacterial or viral infection. Furthermore, supported by a machine learning algorithm, we demonstrated the ability to identify the causative pathogens based on the distinctive host blood plasma glycopeptide signatures. These results illustrate that glycoproteomics holds enormous potential as an innovative approach to improve the interpretation of relevant biological changes in response to infection

31st Annual Meeting and Associated Programs of the Society for Immunotherapy of Cancer (SITC 2016) : part two

Background The immunological escape of tumors represents one of the main ob- stacles to the treatment of malignancies. The blockade of PD-1 or CTLA-4 receptors represented a milestone in the history of immunotherapy. However, immune checkpoint inhibitors seem to be effective in specific cohorts of patients. It has been proposed that their efficacy relies on the presence of an immunological response. Thus, we hypothesized that disruption of the PD-L1/PD-1 axis would synergize with our oncolytic vaccine platform PeptiCRAd. Methods We used murine B16OVA in vivo tumor models and flow cytometry analysis to investigate the immunological background. Results First, we found that high-burden B16OVA tumors were refractory to combination immunotherapy. However, with a more aggressive schedule, tumors with a lower burden were more susceptible to the combination of PeptiCRAd and PD-L1 blockade. The therapy signifi- cantly increased the median survival of mice (Fig. 7). Interestingly, the reduced growth of contralaterally injected B16F10 cells sug- gested the presence of a long lasting immunological memory also against non-targeted antigens. Concerning the functional state of tumor infiltrating lymphocytes (TILs), we found that all the immune therapies would enhance the percentage of activated (PD-1pos TIM- 3neg) T lymphocytes and reduce the amount of exhausted (PD-1pos TIM-3pos) cells compared to placebo. As expected, we found that PeptiCRAd monotherapy could increase the number of antigen spe- cific CD8+ T cells compared to other treatments. However, only the combination with PD-L1 blockade could significantly increase the ra- tio between activated and exhausted pentamer positive cells (p= 0.0058), suggesting that by disrupting the PD-1/PD-L1 axis we could decrease the amount of dysfunctional antigen specific T cells. We ob- served that the anatomical location deeply influenced the state of CD4+ and CD8+ T lymphocytes. In fact, TIM-3 expression was in- creased by 2 fold on TILs compared to splenic and lymphoid T cells. In the CD8+ compartment, the expression of PD-1 on the surface seemed to be restricted to the tumor micro-environment, while CD4 + T cells had a high expression of PD-1 also in lymphoid organs. Interestingly, we found that the levels of PD-1 were significantly higher on CD8+ T cells than on CD4+ T cells into the tumor micro- environment (p < 0.0001). Conclusions In conclusion, we demonstrated that the efficacy of immune check- point inhibitors might be strongly enhanced by their combination with cancer vaccines. PeptiCRAd was able to increase the number of antigen-specific T cells and PD-L1 blockade prevented their exhaus- tion, resulting in long-lasting immunological memory and increased median survival

Genomic investigations of unexplained acute hepatitis in children

Since its first identification in Scotland, over 1,000 cases of unexplained paediatric hepatitis in children have been reported worldwide, including 278 cases in the UK1. Here we report an investigation of 38 cases, 66 age-matched immunocompetent controls and 21 immunocompromised comparator participants, using a combination of genomic, transcriptomic, proteomic and immunohistochemical methods. We detected high levels of adeno-associated virus 2 (AAV2) DNA in the liver, blood, plasma or stool from 27 of 28 cases. We found low levels of adenovirus (HAdV) and human herpesvirus 6B (HHV-6B) in 23 of 31 and 16 of 23, respectively, of the cases tested. By contrast, AAV2 was infrequently detected and at low titre in the blood or the liver from control children with HAdV, even when profoundly immunosuppressed. AAV2, HAdV and HHV-6 phylogeny excluded the emergence of novel strains in cases. Histological analyses of explanted livers showed enrichment for T cells and B lineage cells. Proteomic comparison of liver tissue from cases and healthy controls identified increased expression of HLA class 2, immunoglobulin variable regions and complement proteins. HAdV and AAV2 proteins were not detected in the livers. Instead, we identified AAV2 DNA complexes reflecting both HAdV-mediated and HHV-6B-mediated replication. We hypothesize that high levels of abnormal AAV2 replication products aided by HAdV and, in severe cases, HHV-6B may have triggered immune-mediated hepatic disease in genetically and immunologically predisposed children