Response to Interpreting the results of oceanic mesoscale enrichment experiments: Caveats and lessons from limnology and coastal ecology

Peer Reviewe

The X-ray binary 2S0114+650=LSI+65 010:A slow pulsar or tidally-induced pulsations?

The X-ray source 2S0114+650=LSI+65 010 is a binary system containing a B-type primary and a low mass companion believed to be a neutron star. The system has three reported periodicities: the orbital period, P{orb}~11.6 d, X-ray flaring with P{flare}~2.7 hr, and a "superorbital" X-ray periodicity P{super}~30.7 d. The objective of this paper is to show that the puzzling periodicities in the system may be explained in the context of scenarios in which tidal interactions drive oscillations in the B-supergiant star. We calculate the solution of the equations of motion for one layer of small surface elements distributed along the equator of the star, as they respond to the forces due to gas pressure, centrifugal, coriolis, viscous forces, and the gravitational forces of both stars. This calculation provides variability timescales that can be compared with the observations. In addition, we use observational data obtained at the Observatorio Astron\'omico Nacional en San Pedro M\'artir (OAN/SPM) between 1993-2004 to determine which of the periodicities may be present in the optical region. We suggest that the tidal oscillations lead to a structured stellar wind which, when fed to the neutron star, produces the X-ray modulations. The connection between the stellar oscillations and the modulation of the mass ejection may lie in the shear energy dissipation generated by the tangential motions that are produced by the tidal interaction, particularly in the tidal bulge region. The tidal oscillation scenario weakens the case for 2S0114+650 containing a magnetar descendent.Comment: 12 pages, 14 figure

Polymorphism in TGFB1 is associated with worse non-relapse mortality and overall survival after stem cell transplantation with unrelated donors.

Transforming growth factor beta-1, encoded by the TGFB1 gene, is a cytokine that plays a central role in many physiological and pathogenic processes. We have sequenced TGFB1 regulatory region and assigned allelic genotypes in a large cohort of hematopoietic stem cell transplantation patients and donors. In this study, we analyzed 522 unrelated donor-patient pairs and examined the combined effect of all the common polymorphisms in this genomic region. In univariate analysis, we found that patients carrying a specific allele, 'p001', showed significantly reduced overall survival (5-year overall survival 30.7% for p001/ p001 patients vs. 41.6% others; P=0.032) and increased non-relapse mortality (1-year nonrelapse mortality: 39.0% vs. 25.4%; P=0.039) after transplantation. In multivariate analysis, the presence of a p001/ p001 genotype in patients was confirmed as an independent factor for reduced overall survival [hazard ratio=1.53 (1.04-2.24); P=0.031], and increased non-relapse mortality [hazard ratio=1.73 (1.06-2.83); P=0.030]. In functional experiments we found a trend towards a higher percentage of surface transforming growth factor beta-1-positive regulatory T cells after activation when the cells had a p001 allele (P=0.07). Higher or lower production of transforming growth factor beta-1 in the inflammatory context of hematopoietic stem cell transplantation may influence the development of complications in these patients. Findings indicate that TGFB1 genotype could potentially be of use as a prognostic factor in hematopoietic stem cell transplantation risk assessment algorithms

Actividades prácticas de seguridad alimentaria desarrolladas en terreno. Estrategia de vinculación docente y responsabilidad social

El desarrollo de estrategias educativas en el área de seguridad alimentaria llevadas a cabo en terreno permite al alumnado conocer la situación real de las empresas del sector alimentario. Al mismo tiempo, como el principal objetivo es satisfacer necesidades reales de una comunidad, el trabajo realizado en terreno tiene un impacto directo sobre la sociedad. En el presente trabajo estudiantes de la asignatura Higiene y Seguridad Industrial de la licenciatura en Tecnología de los Alimentos desarrollaron una lista de chequeo como herramienta de auditoría de inocuidad alimentaria para posteriormente aplicarla en terreno evaluando las fortalezas y debilidades de la herramienta desarrollada. La estrategia docente de combinar las actividades teórico-prácticas con actividades prácticas en terreno permitió al alumnado aplicar los conocimientos teóricos en situaciones reales. Finalmente, el alumnado aprendió a realizar una herramienta de trabajo adaptada a las necesidades del sector conforme a la legislación vigente. Por otra parte, el trabajo se desarrolló en el marco de un proyecto de Responsabilidad Social Universitaria (RSU) donde la vinculación entre docentes de distintas asignaturas permitió obtener una formación académica de excelencia y socialmente responsable, donde las iniciativas interdisciplinarias e interinstitucionales fortalecieron la formación profesional de estudiantes de Tecnología de los Alimentos y Veterinaria

EDBL: a General Lexical Basis for the Automatic Processing of Basque

EDBL (Euskararen Datu-Base Lexikala) is a general-purpose lexical database used in Basque text-processing tasks. It is a large repository of lexical knowledge (currently around 80,000 entries) that acts as basis and support in a number of different NLP tasks, thus providing lexical information for several language tools: morphological analysis, spell checking and correction, lemmatization and tagging, syntactic analysis, and so on. It has been designed to be neutral in relation to the different linguistic formalisms, and flexible and open enough to accept new types of information. A browser-based user interface makes the job of consulting the database, correcting and updating entries, adding new ones, etc. easy to the lexicographer. The paper presents the conceptual schema and the main features of the database, along with some problems encountered in its design and implementation in a commercial DBMS. Given the diversity of the lexical entities and the complex relationships existing among them, three total specializations have been defined under the main class of the hierarchy that represents the conceptual schema. The first one divides all the entries in EDBL into Basque standard and non-standard entries. The second divides the units in the database into dictionary entries (classified into the different parts-of-speech) and other entries (mainly non-independent morphemes and irregularly inflected forms). Finally, another total specialization has been established between single-word entries and multiword lexical units; this permits us to describe the morphotactics of single-word entries, and the constitution and surface realization schemas of multiword lexical units.A hierarchy of typed feature structures (FS) has been designed to map the entities and relationships in the database conceptual schema. The FSs are coded in TEI-conformant SGML, and Feature Structure Declarations (FSD) have been made for all the types of the hierarchy. Feature structures are used as a delivery format to export the lexical information from the database. The information coded in this way is subsequently used as input by the different language analysis tools

Influence of Silver Nitrate on Somatic Embryogenesis Induction in Arabica Coffee (Coffea arabica L.).

Plant somatic embryogenesis (SE) has been defined as the formation of embryos from a single or group of haploid or somatic cells [1, 2]. Low frequency (LFSE) and high frequency somatic embryogenesis (HFSE) have been described. In the first type, somatic embryos are induced directly from pro-embryogenic cells of explants, while in the second, they originate from embryogenic callus [1]. It has been suggested that in LFSE the origin of somatic embryos is unicellular, whereas in HFSE has been described as unicellular or multicellular [3]. SE is a powerful biotechnological tool used to propagate elite plants or to conserve important genotypes [4]. Moreover, SE offers an efficient in vitro regeneration approach as a fundamental step in plant genetic improvement for studying basic aspects of ontogenesis of somatic embryos [5]. In Coffea spp., the first studies of SE have been reported at the beginning of 1970 [6]. Since then, a large quantity of LFSE and HFSE protocols have been optimized demonstrating that coffee is not a recalcitrant species for SE [4]. In the LFSE the somatic embryos are obtained faster (approximately 70 days) using only one medium meanwhile in HFSE several media are used and somatic embryo formation takes 9-10 months [4]. Although, in LFSE small number of somatic embryos are obtained (around 10 per explant) compared to hundreds of somatic embryos obtained per gram of embryogenic calli [4], the unicellular origin of somatic embryos in LFSE represents an advantage for the chemical and physical mutagenesis, genetic transformation and genetic editing, since prevents or reduces the appearance of chimeras [7]. In C. arabica and C. canephora many factors (such as genotype, explant type, the physiological state, age and growth conditions of the donor plants, the season of collection, nutrient composition of the medium, the volume of dissolved CO2 or O2 in the culture flask, and plant growth regulators) that affect LFSE induction have been studied [3, 8, 9, 10, 11, 12, 13]. However, few studies reported the effect of silver nitrate on LFSE using leaf explants of C. arabica L. and to the best of our knowledge it has not been analyzed using Caturra and Catuaí, which are two economic important producer cultivars in Costa Rica. Since SE is genotype dependent, the culture medium need to be modified for the different genotypes [7].Therefore, the objective of this study was to determine the influence of the benzyladenine (BAP), indole-3-acetic acid (IAA), and silver nitrate (AgNO3) on low frequency somatic embryogenesis using leaf explants of Coffea arabica L. cultivars Caturra and Catuaí