Doing participative practitioner-based research

Undertaking participative practitioner-based research in educational contexts can be both rewarding and revealing. The author offers some advice concerning the pitfalls and follies of doing this kind of research and how to avoid them

The use of personal narratives to improve organisational life

Andy Armitage and Alan Thornton carried out a piece of research where they used their own experience of university life as data, and created an on-going narrative between themselves as a source of personal and organisational reflection. Such deepening connections could do much to enhance organisational life

Talking about organisations

Can dialogue reveal hidden truths? This is what prompted Bronwen Rees and Andrew Armitage to experiment with the dialogical process to explore how realities and knowledge about our organisations are created

The rapid structured literature review as a research strategy

A diversity of sources of literature encompassed by the management disciplines appears to result in a growing need for a systematic methodology to map the territory of management theory. As such, when scoping out a study, structured literature review (SLR) can be considered as a means by which any critical, central literature might be considered. However, there is little guidance, or evidence, of this being undertaken for the purposes of small scale projects such as undergraduate or masters’ dissertations. This paper reports four case studies of master’s degree students following management programmes of undertaking a structured literature review (SLR) and the issues and problems they had to encounter during their journey. The findings from the case studies suggest that in terms of time to complete and the volume of output required in terms of word count, Tranfield, et al’s approach to SLRs, whilst suited to doctoral level research is not appropriate generally when dealing with undergraduate and masters research projects. Therefore, this paper provides accounts of the experiences of four students who undertook SLR for their undergraduate or master’s degree dissertation. The paper identifies that these students had to deal with a new set of conceptual problems relating to this “unorthodox” approach to a postgraduate research dissertation in coming to terms with new paradigms of enquiry that are not normally taught as part of a traditional research methods course. This was despite gaining a greater depth of insight into the subject area through a more rigorous and structured manner. The paper presents alternative remedies by way of a rapid structured literature review (RSLR) model. This would appear to be more appropriate to the conducting of small scale literature based research projects when used with undergraduate and master’s degree students than SLR identified for other research activities

Undertaking a structured literature review or structuring a literature review: tales from the field

The diversity of sources of literature within the management disciplines has resulted in a growing need for a systematic methodology to map the territory of its associated theories and models. As such, when scoping out a doctoral or policy based study the Structured Literature Review (SLR) can be considered as a means by which critical literature central to and underpinning the research can be rigorously and systematically mapped out. However, there is little guidance, or evidence, of this being the case when undertaking small scale projects for example undergraduate or masters degree dissertations. This paper reports four case studies using semi-structured interviews of masters degree students following management programmes who undertook a Structured Literature Review based dissertation and the issues and problems they had to encounter during their journey. The findings from the case studies suggest that the approach to SLRs, whilst suited to doctoral level and policy based research is not appropriate when dealing with undergraduate and masters dissertations and projects. The case study findings identified that these students conducting a SLR had to deal with a new set of conceptual, methodological and data collection problems relating to this ‘unorthodox’ approach to conducting a postgraduate research dissertation. The findings show that students had to confront new paradigms of enquiry that are not normally taught or found in ‘traditional’ research texts and research methods courses that are taught on degree programmes. However, the findings do reveal that students gained a greater depth and insight into the subject they were researching through a more rigorous and structured approach. The paper then presents alternative remedies by way of the Rapid Structured Literature Review (RSLR) research strategy which is argued as an appropriate approach in conducting small scale literature based research projects when used with undergraduate and masters degree students rather than the SLR, which is better suited for other types of research such as doctoral and policy based activities

CRISPR/Cas9-mediated mutagenesis of phytoene desaturase in diploid and octoploid strawberry

Background: Gene editing using CRISPR/Cas9 is a simple and powerful tool for elucidating genetic controls and for crop improvement and its use has been reported in a growing number of important food crops, including recently Fragaria. In order to inform application of the technology in Fragaria, we targeted the visible endogenous marker gene PDS (phytoene desaturase) in diploid Fragaria vesca ssp. vesca ‘Hawaii 4’ and octoploid F. × ananassa ‘Calypso’. Results: Agrobacterium-mediated transformation of leaf and petiole explants was used for efficient stable integration of constructs expressing plant codon-optimised Cas9 and single guide sequences under control of the Arabidopsis U6-26 consensus promoter and terminator or Fragaria vesca U6III regulatory sequences. More than 80% (‘Hawaii 4’) and 50% (‘Calypso’) putative transgenic shoot lines (multiple shoots derived from a single callus) exhibited mutant phenotypes. Of mutant shoot lines selected for molecular analysis, approximately 75% (‘Hawaii 4’) and 55% (‘Calypso’) included albino regenerants with bi-allelic target sequence variants. Our results indicate the PDS gene is functionally diploid in ‘Calypso’. Conclusion: We demonstrate that CRISPR/Cas9 may be used to generate biallelic mutants at high frequency within the genomes of diploid and octoploid strawberry. The methodology, observations and comprehensive data set pre- sented will facilitate routine application of this technology in Fragaria to single and multiple gene copy targets where mutant phenotypes cannot be identified visually

Critical values for Lawshe's content validity ratio: revisiting the original methods of calculation

YesThe content validity ratio originally proposed by Lawshe is widely used to quantify content validity and yet methods used to calculate the original critical values were never reported. Methods for original calculation of critical values are suggested along with tables of exact binomial probabilities

Bioinformatic characterisation of the effector repertoire of the strawberry pathogen Phytophthora cactorum

The oomycete pathogen Phytophthora cactorum causes crown rot, a major disease of cultivated strawberry. We report the draft genome of P. cactorum isolate 10300, isolated from symptomatic Fragaria x ananassa tissue. Our analysis revealed that there are a large number of genes encoding putative secreted effectors in the genome, including nearly 200 RxLR domain containing effectors, 77 Crinklers (CRN) grouped into 38 families, and numerous apoplastic effectors, such as phytotoxins (PcF proteins) and necrosis inducing proteins. As in other Phytophthora species, the genomic environment of many RxLR and CRN genes differed from core eukaryotic genes, a hallmark of the two-speed genome. We found genes homologous to known Phytophthora infestans avirulence genes including Avr1, Avr3b, Avr4, Avrblb1 and AvrSmira2 indicating effector sequence conservation between Phytophthora species of clade 1a and clade 1c. The reported P. cactorum genome sequence and associated annotations represent a comprehensive resource for avirulence gene discovery in other Phytophthora species from clade 1 and, will facilitate effector informed breeding strategies in other crops

Photometric Accretion Signatures Near the Substellar Boundary

Multi-epoch imaging of the Orion equatorial region by the Sloan Digital Sky Survey has revealed that significant variability in the blue continuum persists into the late-M spectral types, indicating that magnetospheric accretion processes occur below the substellar boundary in the Orion OB1 association. We investigate the strength of the accretion-related continuum veiling by comparing the reddening-invariant colors of the most highly variable stars against those of main sequence M dwarfs and evolutionary models. A gradual decrease in the g band veiling is seen for the cooler and less massive members, as expected for a declining accretion rate with decreasing mass. We also see evidence that the temperature of the accretion shock decreases in the very low mass regime, reflecting a reduction in the energy flux carried by the accretion columns. We find that the near-IR excess attributed to circumstellar disk thermal emission drops rapidly for spectral types later than M4. This is likely due to the decrease in color contrast between the disk and the cooler stellar photosphere. Since accretion, which requires a substantial stellar magnetic field and the presence of a circumstellar disk, is inferred for masses down to 0.05 Msol we surmise that brown dwarfs and low mass stars share a common mode of formation.Comment: 37 pages, 14 figures, accepted by A

Dose-dependent genetic resistance to azole fungicides found in the apple scab pathogen

The evolution of azole resistance in fungal pathogens presents a major challenge in both crop production and human health. Apple orchards across the world are faced with the emergence of azole fungicide resistance in the apple scab pathogen Venturia inaequalis. Target site point mutations observed in this fungus to date cannot fully explain the reduction in sensitivity to azole fungicides. Here, polygenic resistance to tebuconazole was studied across a population of V. inaequalis. Genotyping by sequencing allowed Quantitative Trait Loci (QTLs) mapping to identify the genetic components controlling this fungicide resistance. Dose-dependent genetic resistance was identified, with distinct genetic components contributing to fungicide resistance at different exposure levels. A QTL within linkage group seven explained 65% of the variation in the effective dose required to reduce growth by 50% (ED50). This locus was also involved in resistance at lower fungicide doses (ED10). A second QTL in linkage group one was associated with dose-dependent resistance, explaining 34% of variation at low fungicide doses (ED10), but did not contribute to resistance at higher doses (ED50 and ED90). Within QTL regions, non-synonymous mutations were observed in several ATP-Binding Cassette and Major Facilitator SuperFamily transporter genes. These findings provide insight into the mechanisms of fungicide resistance that have evolved in horticultural pathogens. Identification of resistance gene candidates supports the development of molecular diagnostics to inform management practices