Cavitation erosion fracture mechanisms and their detection in ship rudders

The phenomenon of cavitation is of great importance when ship propellers and rudders are considered, as it can often be the cause of vibrations, noise, reduced efficiency and even erosion in some instances. The underlying fracture mechanisms of erosion, however, have not been fully understood yet. As such, this study aims to expand our knowledge regarding the fracture mechanisms of common shipbuilding alloys and explore whether cavitation erosion can be monitored, by using the relevant quantitative and qualitative data. As such, an experimental test rig was built, based on the induction of cavitation by ultrasonic means, in order for a series of tests, including mass loss and acoustic emission measurements as well as microscopic observations to be conducted. Due to the interest of BAE Systems, a number of protective coatings were also examined under an analogous context. Specimens were initially exposed to ultrasonically induced cavitation under identical experimental conditions. Mass loss was periodically measured thus materials were categorized in that respect while the positive effect of cathodic protection on the resulting erosion was confirmed. Examination through optical and scanning electron microscopes was also conducted thus the fracture mechanisms and macroscopic characteristics of cavitation erosion were identified, for each of the examined materials. Results showed that, erosion initiates through plastic deformation (orange peeling) before proceeding into ductile and brittle, due to work hardening, fracture, whereas the extent and crack propagation characteristics of each phase, depend on the material’s mechanical properties and crystalline structure. Acoustic emissions were also examined, with the aim of, characterizing the materials and potentially be utilized for erosion monitoring. Upon the successful establishment of acoustic thresholds for cavitation erosion, in the case of small specimens, a small model rudder was also examined under an analogous context, although in that instance, cavitation localization was also considered, through a triangulation source location technique. In that instance, cavitation induced erosion, was effectively monitored and characterized both in terms of intensity and location. A model rudder twice as large as the small one was also examined in order for any possible scale effects to be identified. Cavitation induced erosion, was again effectively monitored, both in terms of intensity and location, although results indicated that the method should be optimized, with respect to the parameter of size. As such, the future researcher could further promote the evolvement of the aforementioned ship rudder monitoring system, by means of optimizing the analytical procedures in order to overcome any possible scale effects, further adapting the characteristics of the system to match the size of the objects to be monitored and eventually lead to the full – scale application of the system. The conduction of sea trials would also be of great benefit and importance towards the direction of forming a solid cavitation erosion monitoring system

Fibre Bragg Grating-based Acoustic Sensor Array for Improved Condition Monitoring of Marine Lifting Surfaces

This paper discusses a novel approach to monitor marine lifting surface conditions through using arrays of Fibre Bragg Grating (FBG)-based acoustic sensors in a marine rudder. Results from these optical sensor arrays are then cross compared with those from conventional piezoelectric (PZT) sensors. A successful proof-of-concept evaluation of the optical sensor approach was, however, first undertaken by using cascaded FBGs integrated into a glass plate, monitoring the response to dropping a standard metal ball at different locations. Data obtained were compared with colocated conventional PZT sensors acoustic sensors for comparison using triangulation to determine the location of the excitation source (a sonotrode). The results obtained verify the excellent performance of the FBG-based sensors due to the excellent agreement between these different sensor types. This gives confidence to the next-stage to scale-up the FBG sensor arrays for other marine structures, with early identification of the initiation of cavitation erosion an important priority for better operational reliability and scheduling of maintenance of marine vessels

Fibre Bragg Grating-based Cascaded Acoustic Sensors for Potential Marine Structural Condition Monitoring

This paper explores the potential of using multiple Fibre Bragg grating (FBG)-based sensors for acoustic emission (AE) detection, thus offering an effective alternative to conventional piezoelectric (PZT) sensors, especially where they have shown limitations in use, such as in the marine sector. A cascaded fibre optic acoustic sensor system, using optical filter signal demodulation has been developed and its performance extensively evaluated. To undertake this under standardized conditions, the optical sensor system was evaluated using a glass plate to detect the acoustic signal, followed by an evaluation using a metal plate to identify the location of acoustic sources, when subjected to sonotrode excitation, mimicking acoustic detection in cavitation detection. Under these circumstances, a very good agreement has been reached between the outputs of the optical acoustic sensors and of the co-located PZT acoustic sensors. This work confirms the utility of these sensors – they can detect not only weak AE signals, but also enable multipoint simultaneous measurement, showing their potential for condition monitoring applications, especially in the marine sector

Prevalence of BRCA1 and BRCA2 mutations in unselected breast cancer patients from Greece

<p>Abstract</p> <p>Background</p> <p>Inheritance of a mutation in either <it>BRCA1 </it>or <it>BRCA2 </it>accounts for approximately 5% of all breast cancer cases, but varies by country. Investigations into the contribution of <it>BRCA </it>mutations to breast cancer incidence in Greece have been, for the most part, limited by small sample sizes and by the use of cases selected for their family history of cancer. The aim of the current study was to estimate <it>BRCA </it>mutation frequencies in breast cancer patients unselected for family history.</p> <p>Methods</p> <p>To do so, we enrolled 127 unselected women with breast cancer from the Alexandra Hospital in Athens, Greece, a large public hospital in the city. Mutations in <it>BRCA1 </it>and <it>BRCA2 </it>were detected using a combination of techniques and were confirmed by direct sequencing. Two large genomic deletions were sought using mutation-specific assays. A detailed family history of cancer was obtained from each patient.</p> <p>Results</p> <p>We were able to successfully complete testing on samples from 127 women. Among these, six mutations were identified (four in <it>BRCA1 </it>and two in <it>BRCA2</it>) representing 4.7% of the total or 9.5% of cases diagnosed before age forty. None of the mutation carriers had a family history of breast or ovarian cancer. Three of the four <it>BRCA1 </it>mutations were in exon 20: two were a G5331A mutation and the third was a 3.2 kb deletion. The fourth <it>BRCA1 </it>mutation was the 3819delGTAAA in exon 11. The two <it>BRCA2 </it>mutations were in exon 11 (3782del10 and 4512insT).</p> <p>Conclusions</p> <p>The G5331A mutation in <it>BRCA1 </it>appears to be a founder mutation in the Greek population.</p

Low Frequency Vibrations Disrupt Left-Right Patterning in the Xenopus Embryo

The development of consistent left-right (LR) asymmetry across phyla is a fascinating question in biology. While many pharmacological and molecular approaches have been used to explore molecular mechanisms, it has proven difficult to exert precise temporal control over functional perturbations. Here, we took advantage of acoustical vibration to disrupt LR patterning in Xenopus embryos during tightly-circumscribed periods of development. Exposure to several low frequencies induced specific randomization of three internal organs (heterotaxia). Investigating one frequency (7 Hz), we found two discrete periods of sensitivity to vibration; during the first period, vibration affected the same LR pathway as nocodazole, while during the second period, vibration affected the integrity of the epithelial barrier; both are required for normal LR patterning. Our results indicate that low frequency vibrations disrupt two steps in the early LR pathway: the orientation of the LR axis with the other two axes, and the amplification/restriction of downstream LR signals to asymmetric organs

Kisspeptin effect on endothelial monocyte activating polypeptide II (EMAP-II)-associated lymphocyte cell death and metastases in colorectal cancer patients

Kisspeptin is an antimetastatic agent in some cancers that has also been associated with lymphoid cell apoptosis, a phenomenon favoring metastases. Our aim was to determine the association of kisspeptin with lymphocyte apoptosis and the presence of metastases in colorectal cancer patients. Blood was drawn from 69 colon cancer patients and 20 healthy volunteers. Tissue specimens from healthy and pathological tissue were immunohistochemically analyzed for kisspeptin and endothelial monocyte activating polypeptide II (EMAP-II) expression. Blood EMAP-II and soluble Fas ligand (sFasL) levels were examined by an enzymelinked immunosorbent assay method. The kisspeptin and EMAP-II expression and secretion levels in the DLD-1 and HT-29 colon cancer cell lines were examined by quantitative real-time polymerase chain reaction, Western analysis and enzyme-linked immunosorbent assay, whereas lymphocyte viability was assessed by flow cytometry. The effect of kisspeptin on the viability of colon cancer cells was examined by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide]. Exogenous, synthetic and naturally produced, kisspeptin induces through the G-protein-coupled receptor 54 (GPR54; also known as the kisspeptin receptor) the EMAP-II expression and secretion in colon cancer cell lines, inducing in vitro lymphocyte apoptosis, as verified by the use of an anti-EMAP-II antibody. These results were reversed with the use of kisspeptin inhibitors and by kisspeptin-silencing experiments. Tumor kisspeptin expression was associated with the tumor EMAP-II expression (p &lt; 0.001). Elevated kisspeptin and EMAP-II expression in colon cancer tissues was associated with lack of metastases (p &lt; 0.001) in colon cancer patients. These data indicate the antimetastatic effect of tumor-elevated kisspeptin in colon cancer patients that may be mediated by the effect of kisspeptin on EMAP-II expression in colon cancer tumors in patients with normal serum EMAP-II levels. These findings provide new insight into the role of kisspeptin in the context of metastases in colon cancer patients