Una propuesta tecnológica basada en radiofrecuencia para apoyar el proceso de picking en los cuartos fríos de Industria de Alimentos Zenú S.A.S

El presente trabajo aborda una propuesta para la implementación de una herramienta tecnológica basada en la radio frecuencia, para apoyar la operación logística que se realiza en la compañía Industria de Alimentos Zenú S.A.S.En el centro de distribución Regional Pereira. Esta propuesta tiene como objetivo principal, beneficiar y optimizar los recursos de la organización, aumentando la productividad (Kilos hora hombre), eficiencia en la operación logística, y flexibilidad en los horarios para responder a comportamientos imprevisibles. Por tal motivo, es relevante apoyarse en las herramientas tecnológicas del momento para mejorar dichos procesos logísticos. En contraprestación de lo anterior, el sistema de radio frecuencia es una nueva herramienta que soporta el proceso de separación PICKING. Dicha propuesta se soporta en primer lugar con la caracterización del sistema logístico que se maneja en la empresa actualmente con su correspondiente análisis descriptivo. En segundo lugar,se trabaja con el objetivo claro de desarrollar la propuesta de la implementación de la radiofrecuencia, en tercer lugar se mencionan las ventajas de la implementación de la herramienta, realizando una contraste entre el sistema actual y el propuesto y por último se realiza un análisis de costos para dicha implementación. Estos conceptos se enmarcan en los cuartos fríos del centro de distribución. Con el fin de poder lograr parte de la solución, por medio de conceptos llevados a la práctica, según el contexto del planteamiento del problema encontrado y su enfoque deeliminar prácticas ineficientes, llevadas actualmente en el proceso de separación “Picking” que se reflejan en el personal y en el factor productivo insatisfecho

Biotecnología aplicada en el control y manipulación del ciclo estral de bovinos.

El objetivo del presente proyecto de investigación fue recabar información acerca de las biotecnologías reproductivas aplicadas en el control y manipulación del ciclo estral de bovinos. Para poder llevar a cabo esta investigación se realizó la recopilación de información de artículos científicos, tesis, libros consultando las bases de datos de las siguientes plataformas científicas: E-book, RESEARCHGATE, SciELO, Springer, LA Referencia, DSpace ESPOCH, redalyc, Dialnet. Se han revisado artículos con los temas: los mecanismos y herramientas que regulan el ciclo estral, biotecnologías reproductivas aplicadas al control del ciclo estral y las ventajas de aplicar biotecnologías reproductivas en las ganaderías. En cuanto a los mecanismos y herramientas que regulan el ciclo estral involucra varios órganos: el eje hipotálamo-hipófisis. Ovarios, útero y las hormonas reproductivas. El uso de biotecnologías aplicadas en la reproducción como la sincronización e inducción del estro y de la ovulación ha permitido mejorar la eficiencia reproductiva de las hembras bovinas. Finalmente, los procesos fisiológicos que se llevan dentro del sistema reproductivo de la hembra bovina para poder lograr la preñez son llevados a cabo por eventos hormonales regulados por el hipotálamo mediante la secreción de GnRH, la hipófisis secreta FSH y la LH, el folículo secreta estrógenos e inhibina, el cuerpo lúteo secreta progesterona y el útero produce prostaglandina. Aplicar una serie de hormonas como la GnRH Estradiol y Progesterona para la sincronización de la onda folicular; PgF2α y dispositivos de progesterona para el control de la fase luteal; la GnRH, Estradiol y LH para la inducción de la ovulación, estas combinaciones hormonales permiten manipular la fisiología reproductiva de la vaca lo cual permite gobernar su ciclo estral. Por esta razón las biotecnologías reproductivas permiten obtener los mejores rendimientos productivos y reproductivos de las explotaciones ganaderas.This research got information about reproductive biotechnologies applied in the control and manipulation of the bovine estrous cycle. The development of this study collected data from scientific articles, theses, books, recipients of the databases of the following scientific platforms: E-book, RESEARCHGATE, SciELO, Springer, LA Reference, DSpace ESPOCH, redalyc, Dialnet. Articles of different topics were reviewed as mechanisms and tools that regulate the estrous cycle, reproductive biotechnologies applied to estrous cycle control and, the advantages of apply reproductive biotechnologies in livestock farms. Several organs involve the mechanisms and tools that regulated the estrous cycle: the hypothalamus-pituitary axis, ovaries, uterus and, reproductive hormones. The use of biotechnologies applied in reproduction as synchronization and induction of estrus and ovulation allowed improving the reproductive efficiency of bovine females. Finally, the physiological processes carried out within the bovine female reproductive system to achieve pregnancy through hormonal events regulated by the hypothalamus through the secretion of GnRH, the pituitary secretes FSH and LH; the follicle secretes estrogens and inhibin. The corpus luteum secretes progesterone and, the uterus produces prostaglandin. Applying a series of hormones such as GnRH Estradiol and Progesterone for synchronization of the follicular wave; PgF2α and progesterone devices for control of the luteal phase; GnRH, Estradiol and, LH for ovulation induction, these hormonal combinations allow manipulation of the cow's reproductive physiology which control the estrous cycle. For this reason, reproductive biotechnologies help to obtain the best productive and reproductive performances in cattle farms

Transcription factor NFE2L2/NRF2 modulates chaperone-mediated autophagy through the regulation of LAMP2A

Chaperone-mediated autophagy (CMA) is a selective degradative process for cytosolic proteins that contributes to the maintenance of proteostasis. The signaling mechanisms that control CMA are not fully understood but might involve response to stress conditions including oxidative stress. Considering the role of CMA in redoxtasis and proteostasis, we sought to determine if the transcription factor NFE2L2/NRF2 (nuclear factor, erythroid derived 2, like 2) has an impact on CMA modulation. In this work, we identified and validated 2 NFE2L2 binding sequences in the LAMP2 gene and demonstrated in several human and mouse cell types that NFE2L2 deficiency and overexpression was linked to reduced and increased LAMP2A levels, respectively. Accordingly, lysosomal LAMP2A levels were drastically reduced in nfe2l2-knockout hepatocytes, which also displayed a marked decrease in CMA activity. Oxidant challenge with paraquat or hydrogen peroxide, or pharmacological activation of NFE2L2 with sulforaphane or dimethyl fumarate also increased LAMP2A levels and CMA activity. Overall, our study identifies for the first time basal and inducible regulation of LAMP2A, and consequently CMA activity, by NFE2L2. Abbreviations: ACTB: actin, beta, ARE: antioxidant response element; ATG5: autophagy related 5; BACH1: BTB domain and CNC homolog 1; ChIP: chromatin immunoprecipitation; CMA: chaperone-mediated autophagy; DHE: dihydroethidium; DMF: dimethyl fumarate; ENCODE: Encyclopedia of DNA elements at the University of California, Santa Cruz; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; GBA: glucosylceramidase beta; GFP: green fluorescent protein; HMOX1: heme oxygenase 1; H2O2: hydrogen peroxide; HSPA8/HSC70: heat shock protein family A (Hsp70) member 8; KEAP1: kelch like ECH associated protein 1; LAMP2A: lysosomal associated membrane protein 2A; LAMP2B: lysosomal associated membrane protein 2B; LAMP2C: lysosomal associated membrane protein 2C; LAMP1: lysosomal associated membrane protein 1; MAFF: MAF bZIP transcription factor F; MAFK: MAF bZIP transcription factor K; NFE2L2/NRF2: nuclear factor, erythroid derived 2, like 2; NQO1: NAD(P)H quinone dehydrogenase 1; PQ: paraquat; PI: protease inhibitors; qRT-PCR: quantitative real-time polymerase chain reaction; RNASE: ribonuclease A family member; SFN: sulforaphane; SQSTM1/p62: sequestosome 1; TBP: TATA-box binding proteinThis paper was funded by the Spanish Ministry of Economy and Competitiveness under the grant SAF2016-76520-R and by grants from the National Institute of Health/National Institute on Aging P01 AG031782. M.P. is recipient of a FPU fellowship of Autonomous University of Madrid. E.A. was supported by NIH/NIA AG038072 P&

The Impact of Sleep Quality on Mood Status and Quality of Life in Patients with Alopecia Areata: A Comparative Study

Alopecia Areata (AA) is a chronic condition which has been associated with poor quality of life and mood status disturbances. The aim of this study is to compare the sleep quality between AA patients and controls, and to analyze the impact of poor sleep quality on patients with AA regarding mood status disturbances, quality of life and sexuality. A cross-sectional study including patients suffering from mild-to-severe AA and sex- and age-matched healthy controls was performed. Socio-demographic and clinical variables, sleep quality, quality of life, sexual disfunction, anxiety, depression and personality were collected using validated questionnaires. A total of 120 participants (60 patients and 60 controls) were included. Patients with AA showed worse sleep scores than controls (p = 0.003), as well as higher rates of anxiety and depression (p < 0.05). After a multivariate analysis, a worse sleep quality was found to be linked to anxiety, depression, a poorer quality of life and a type D personality score independently of the disease severity. In light of the results, patients with AA have a worse sleep quality than healthy controls. A poor sleep quality is associated with anxiety, depression and a worse quality of life, therefore being a general marker of a poor quality of life. Screening for sleep disturbances in specialized units could be useful to detect patients who could benefit from additional psychological support

Quality of Life and Mood Status Disturbances in Cohabitants of Patients with Alopecia Areata: A Cross-Sectional Study in a Spanish Population

A poor quality of life has been described in patients suffering from Alopecia Areata (AA). However, there is little evidence on how AA can impact on those living with patients. The aim of this study is to analyze the impact of AA on a cohabitant’s quality-of-life, mood status disturbances and sexual satisfaction. This is a cross-sectional study of AA patients and their cohabitants. Sociodemographic variables and disease severity, the quality of life, mood status disturbances and sexual dysfunction were collected using validated questionnaires. Eighty-four subjects were included in the study: 42 AA patients and 42 cohabitants. A poor quality of life and worse disease control in the patients were associated with a poorer quality of life of the family, higher scores of anxiety and depression, and the lower sexual satisfaction of cohabitants (p < 0.05). Anxiety and depression in patients were associated with worse family quality of life, higher rates of anxiety and less sexual satisfaction in cohabitants (p < 0.05). To conclude, AA seems to have an impact on the quality of life of cohabitants, leading to increased rates of anxiety, depression, a poorer quality of life, and reduced sexual satisfaction. In light of the results, a global approach for AA patients, including the care of the people who live with them, should be implemented

Impact of the COVID-19 Pandemic on the Diagnosis and Prognosis of Melanoma

Background: Early detection of melanoma is one of the main diagnostic goals of dermatologists worldwide, due to the increasing incidence of the disease in our environment. However, the irruption of the SARS-CoV-2 pandemic has posed a challenge to global healthcare, forcing systems to focus their resources on the fight against COVID-19. Methods: Retrospective cohort study. The exposed cohort were patients diagnosed with melanoma in the year after the general confinement in Spain (15 March 2020) and the unexposed cohort were patients with melanoma diagnosed in the previous year. Results: 130 patients were included. No differences were observed between demographic characteristics in both cohorts. The mean Breslow of melanoma before the onset of the pandemic was 1.08, increasing to 2.65 in the year after the onset of the pandemic (p < 0.001). On the other hand, the percentage of melanomas in situ decreased from 38.96% to 16.98% in the year after the declaration of the state of alarm in Spain. Conclusions: The SARS-CoV-2 outbreak has led to a reduction in the early diagnosis of melanoma, with an increase in invasive melanomas with poor prognosis histological factors. This could lead to an increase in melanoma-related mortality in the coming years in our environment

Taquicardia ventricular polimórfica catecolaminérgica en adolescente: un diagnóstico clínico, electrocardiográfico y genético

Catecholaminergic polymorphic ventricular tachycardia is one of the most lethal channelopathies, characterized by ventricular arrhythmias triggered by stress or physical activity. We present the case of an adolescent who consulted for recurrent syncope precipitated by exercise. In the diagnostic approach, catecholaminergic polymorphic ventricular tachycardia was reached, with a mutation in the cardiac ryanodine receptor gene, Heterozygous c.14311G&gt; A (p.v4771I exon 100), antiarrhythmic drugs and implantable cardioverter-defibrillator were necessary with satisfactory evolution. Clinical suspicion, stress test and genetic tests are essential for a timely diagnosis and management of this pathology.La taquicardia ventricular polimórfica catecolaminérgica es una de las canalopatías más letales. Los síntomas de la enfermedad aparecen en la niñez o la adolescencia, los cuales están caracterizados por arritmias ventriculares desencadenadas por estrés o actividad física. Se presenta el caso de una adolescente que consultó por síncopes recurrentes precipitados por el ejercicio. En el abordaje diagnóstico se determinó como taquicardia ventricular polimórfica catecolaminérgica, con mutación en el gen del receptor de la rianodina cardíaco, heterocigoto c.14311G&gt;A(p.v4771I exón 100), para el manejo fue necesario antiarrítmicos y el implante de un cardiodesfibrilador, con evolución satisfactoria. La sospecha clínica, la prueba de esfuerzo y las pruebas genéticas son fundamentales para un diagnóstico y manejo oportuno de esta patología

Type D Personality Is Associated with Poorer Quality of Life in Patients with Chronic Spontaneous Urticaria: A Cross-sectional Study

Type D personality (TDp) is a stable personality type that has been associated with poor quality of life in the general population and in patients with a variety of diseases, such as cancer, cardiac diseases, and dermatological diseases (e.g. psoriasis). To date, the potential association between chronic spontaneous urticaria and TDp has not been studied. The aim of this study is to analyse the impact of TDp on patients with chronic spontaneous urticaria, regarding mood disturbances, quality of life, sexuality, and sleep disturbances. A cross-sectional study including 75 patients with chronic spontaneous urticaria was performed. Data on sociodemographic variables and disease activity, quality of life, sleep, sexual dysfunction, anxiety, depression and TDp were collected using validated questionnaires. TDp was present in 28% (21/75) of the patients. Although TDp was not related to worse disease control, the presence of anxiety and depression was higher in patients with TDp. Regarding quality of life, TDp was associated with poorer quality of life and higher frequency of sleep disturbances. Prevalence of TDp in patients with chronic spontaneous urticaria is similar to that in the general population. It is associated with mood status disturbances and worse quality of life regardless of disease severity, especially in the emotional and psychological domains. This group of patients could benefit from additional psychological support as a complement to their medical treatment

Towards Compensable SLAs

In Cooperative Information Systems, service level agreements (SLA) can be used to describe the rights and obligations of parties involved in the transaction (typically the service consumer and the service provider); amongst other information, SLA could define guarantees associated with the idea of service level objectives (SLOs) that normally represent key performance indicators of either the consumer or the provider. in case the guarantee is under-fulfilled or over-fulfilled SLAs could also define some compensations (i.e. penalties or rewards). in such a context, during the last years there have been important steps towards the automation of the management of SLAs, however the formalization of compensations in SLAs still remains as an important challenge. in this paper we aim to provide a characterization model to create SLAs with compensations; specifically, the main contributions are twofold: (i) the conceptualization of the Compensation Function to express consistently penalties and rewards and (ii) a model for Compensable Guarantees that associate SLOs with Compensation Functions. This formalization models aims to establish a foundation to elaborate tools that could provide an automated support to the modeling and analysis of SLAs with compensations. Additionally, in order to validate our approach, we model and analyze a set of guarantee terms from three real world examples of SLAs and our formalization proves to be useful for detecting mistakes that are ty