Prismatic to asbestiform offretite from Northern Italy. Occurrence, morphology and crystal-chemistry of a new potentially hazardous zeolite

A multi-methodological approach, based upon field investigation, morphological characterization, chemical analysis and structure refinement was applied to different samples of fibrous offretite, a new potentially hazardous zeolite recently discovered in northern Italy. Their morphology ranges from stocky-prismatic to asbestiform. All the investigated fibers may be considered as "inhalable", and they are well within the range of the "more carcinogenic fibers" regarding diameter. As regards the length, the main mode observed in the asbestiform samples is 20-25 mu m, and similar to 93% of the measured fibers are >5 mu m and may be significantly associated with carcinogenesis also in terms of lengths. The chemical-structural features of the investigated fibers are comparable: the extra-framework cations K+, Mg2+ and Ca2+ are present in all samples in similar proportions, and refined cell parameters are similar among the samples. Offretite occurs in 60% of the investigated sites, with an estimated amount up to 75 vol % of the associated minerals. The presence of this mineral could be of concern for risk to human health, especially if one considers the vast number of quarries and mining-related activities that are operating in the zeolite host rocks

L’apprendimento della letto-scrittura e i fattori di rischio: un progetto sulla consapevolezza degli insegnanti

Reports of suspected Learning Disorders are significantly increased even when it comes to problems that must be addressed with specific teaching actions. To meet that need, born the pilot project for research-training, financed by the Umbria Region, and that involves the Regional Scholastic Office and the local health authorities of Perugia and Terni, is meant to accompany a championof teachers of the first classes of primary school in the acquisition of a more refined professional skill with respect to the reading and writing, and early identification of risk factors.Research has highlighted the importance of building a methodology and teaching based on observation and documentation. These tools are able to tell the story of every child learns to read and write, in an open setting to learning social perspective and respectful of the times of each. The pilot project will be extended this year to other teachers of other educational institutions andtrained teachers will become, in turn, co-trainers of colleagues in their own educational institution.Reports of suspected Learning Disorders are significantly increased even when it comes to problems that must be addressed with specific teaching actions. To meet that need, born the pilot project for research-training, financed by the Umbria Region, and that involves the Regional Scholastic Office and the local health authorities of Perugia and Terni, is meant to accompany a championof teachers of the first classes of primary school in the acquisition of a more refined professional skill with respect to the reading and writing, and early identification of risk factors.Research has highlighted the importance of building a methodology and teaching based on observation and documentation. These tools are able to tell the story of every child learns to read and write, in an open setting to learning social perspective and respectful of the times of each. The pilot project will be extended this year to other teachers of other educational institutions andtrained teachers will become, in turn, co-trainers of colleagues in their own educational institution

Prismatic to asbestiform offretite from Northern Italy. Occurrence, morphology and crystal-chemistry of a new potentially hazardous zeolite

A multi-methodological approach, based upon field investigation, morphological characterization, chemical analysis and structure refinement was applied to different samples of fibrous offretite, a new potentially hazardous zeolite recently discovered in northern Italy. Their morphology ranges from stocky-prismatic to asbestiform. All the investigated fibers may be considered as "inhalable", and they are well within the range of the "more carcinogenic fibers" regarding diameter. As regards the length, the main mode observed in the asbestiform samples is 20-25 mu m, and similar to 93% of the measured fibers are >5 mu m and may be significantly associated with carcinogenesis also in terms of lengths. The chemical-structural features of the investigated fibers are comparable: the extra-framework cations K+, Mg2+ and Ca2+ are present in all samples in similar proportions, and refined cell parameters are similar among the samples. Offretite occurs in 60% of the investigated sites, with an estimated amount up to 75 vol % of the associated minerals. The presence of this mineral could be of concern for risk to human health, especially if one considers the vast number of quarries and mining-related activities that are operating in the zeolite host rocks

ERG conductance expression modulates the excitability of ventral horn GABAergic interneurons that control rhythmic oscillations in the developing mouse spinal cord

During antenatal development, the operation and maturation of mammalian spinal networks strongly depend on the activity of ventral horn GABAergic interneurons that mediate excitation first and inhibition later. Although the functional consequence of GABA actions may depend on maturational processes in target neurons, it is also likely that evolving changes in GABAergic transmission require fine-tuning in GABA release, probably via certain intrinsic mechanisms regulating GABAergic neuron excitability at different embryonic stages. Nevertheless, it has not been possible, to date, to identify certain ionic conductances upregulated or downregulated before birth in such cells. By using an experimental model with either mouse organotypic spinal cultures or isolated spinal cord preparations, the present study examined the role of the ERG current (IK(ERG) ), a potassium conductance expressed by developing, GABA-immunoreactive spinal neurons. In organotypic cultures, only ventral interneurons with fast adaptation and GABA immunoreactivity, and only after 1 week in culture, were transformed into high-frequency bursters by E4031, a selective inhibitor of IK(ERG) that also prolonged and made more regular spontaneous bursts. In the isolated spinal cord in which GABA immunoreactivity and m-erg mRNA were colocalized in interneurons, ventral root rhythms evoked by NMDA plus 5-hydroxytryptamine were stabilized and synchronized by E4031. All of these effects were lost after 2 weeks in culture or before birth in coincidence with decreased m-erg expression. These data suggest that, during an early stage of spinal cord development, the excitability of GABAergic ventral interneurons important for circuit maturation depended, at least in part, on the function of IK(ERG)

Clinicopathological findings and risk factors associated with Cytauxzoon spp. infection in cats : A case-control study (2008-2021)

In Europe, Cytauxzoon spp. infection was documented in domestic and wild felids. Cats often develop a subclinical infection, while fatal disease is rare. Currently, information on the epidemiology, risk factors and clinicopathological findings of Cytauxzoon spp. infection remains limited and obtained by a single subject or small groups of cats. The objective of this case-control study was to evaluate clinicopathological findings and to describe risk factors associated with Cytauxzoon spp. infection in domestic cats. Infected cats (n = 39) and non-infected (n = 190) cats were selected from the database of the referral San Marco Veterinary Laboratory between 2008 and 2021. Demographic information, a preset questionnaire considering lifestyle, environment, and clinical status, and a CBC performed contextually with the PCR analysis were recorded for all cats. Data on the biochemical profile and serum protein electrophoresis were also evaluated when available. Compared to the control group, infection was more likely to occur in stray cats (24/39, 61.5%, P < 0.001), living totally/partially outdoors (36/39, 92.3%, P < 0.001), in an urban context (37/39, 94.9%, P = 0.002), taken or recently adopted from colonies (34/35, 97.1, P < 0.001), with irregular or absent parasite preventive treatments (39/39, 100%, p = 0.005), without fleas (28/35, 80%, P = 0.047) and without clinical signs (22/39, 56.4%, p = 0.026) at the time of medical evaluation. Anemia was not associated with infection, but in cats without clinical signs, the percentage of anemic-infected cats (7/22, 31.8%, P = 0.009) was higher compared to non-infected cats (5/65, 7.7%). Furthermore, a decrease in total iron serum concentration approximating the lowest reference interval [median values (IQR): 79 μg/dL (52.25) vs. 50.5 μg/dL (34), P = 0.007] was likely in infected cats. No other laboratory findings were associated with infection. Interestingly, a partial/total outdoor lifestyle was a risk factor for infection (OR: 8.58, 95% CI: 2.90-37.0, P < 0.001). In conclusion, the present study revealed that Cytauxzoon spp. infection manifests itself prevalently as a subclinical infection, based on physical examination and laboratory findings, in domestic European cats. However, subclinical infected cats were more likely to be anemic compared to non-infected

Survey, characterization and antimicrobial susceptibility of Clostridium difficile from marine bivalve shellfish of North Adriatic Sea

Abstract Clostridium difficile is a major cause of infectious diarrhea associated to healthcare settings. Community-acquired infections are increasingly reported in the last decade and exposure other than to symptomatic patients rather to contaminated foods or animals is feasible. Occurrence of C. difficile in shellfish raises concern because spores can survive the cooking temperatures given that shellfish is often consumed poorly cooked or raw. Aim of our study was to investigate whether shellfish represents a reservoir of C. difficile human PCR-ribotypes (RTs). 702 shellfish samples of farmed and wild bivalve mollusc species were collected over the 2015–2017 period in North Adriatic Italian Sea to investigate contamination with C. difficile and characterize the isolates in terms of genotypic variability and antimicrobial resistance profile. C. difficile was detected in 16.9% (CI: 14.1%–19.8%) samples: 11.6% mussels and 23.2% clams. Compared to mussels, clams were significantly associated with detection of C. difficile (OR = 2.4, P   ECOFF for vancomycin. C. difficile strains showed high variety in RTs, most of them already detected in other animals or known as highly virulent and epidemic in humans. These results prompt towards investigating on specific risk mitigation measures against C. difficile and are preliminary for any source attribution and risk assessment study

Climate of an Ultra hot Jupiter: Spectroscopic phase curve of WASP-18b with HST/WFC3

We present the analysis of a full-orbit, spectroscopic phase curve of the ultra hot Jupiter WASP-18b, obtained with the Wide Field Camera 3 aboard the Hubble Space Telescope. We measure the planet's normalized day-night contrast as >0.96 in luminosity: the disk-integrated dayside emission from the planet is at 964+-25 ppm, corresponding to 2894+-30 K, and we place an upper limit on the nightside emission of <32ppm or 1430K at the 3-sigma level. We also find that the peak of the phase curve exhibits a small, but significant offset in brightness of 4.5+-0.5 degrees eastward. We compare the extracted phase curve and phase resolved spectra to 3D Global Circulation Models and find that broadly the data can be well reproduced by some of these models. We find from this comparison several constraints on the atmospheric properties of the planet. Firstly we find that we need efficient drag to explain the very inefficient day-night re-circulation observed. We demonstrate that this drag could be due to Lorentz-force drag by a magnetic field as weak as 10 Gauss. Secondly, we show that a high metallicity is not required to match the large day-night temperature contrast. In fact, the effect of metallicity on the phase curve is different from cooler gas-giant counterparts, due to the high-temperature chemistry in WASP-18b's atmosphere. Additionally, we compare the current UHJ spectroscopic phase curves, WASP-18b and WASP-103b, and show that these two planets provide a consistent picture with remarkable similarities in their measured and inferred properties. However, key differences in these properties, such as their brightness offsets and radius anomalies, suggest that UHJ could be used to separate between competing theories for the inflation of gas-giant planets.Comment: Accepted for publication in A&

A HST/WFC3 Thermal Emission Spectrum of the Hot Jupiter HAT-P-7b

Secondary eclipse observations of several of the hottest hot Jupiters show featureless, blackbody-like spectra or molecular emission features, which are consistent with thermal inversions being present in those atmospheres. Theory predicts a transition between warmer atmospheres with thermal inversions and cooler atmospheres without inversions, but the exact transition point is unknown. In order to further investigate this issue, we observed two secondary eclipses of the hot Jupiter HAT-P-7b with the Hubble Space Telescope (HST) WFC3 instrument and combined these data with previous Spitzer and Kepler secondary eclipse observations. The HST and Spitzer data can be well fit by a blackbody with $T=2692 \pm 14$ K, and the Kepler data point constrains the geometric albedo to $A_{g}=0.077 \pm 0.006$. We modeled these data with a 3D GCM and 1D self-consistent forward models. The 1D models indicate that the atmosphere has a thermal inversion, weak heat redistribution, and water dissociation that limits the range of pressures probed. This result suggests that WFC3 observations of HAT-P-7b and possibly some other ultra-hot Jupiters appear blackbody-like because they probe a region near the tropopause where the atmospheric temperature changes slowly with pressure. Additionally, the 1D models constrain the atmospheric metallicity ($[\text{M/H}]=-0.87^{+0.38}_{-0.34}$) and the carbon-to-oxygen ratio (C/O $<1$ at 99 % confidence). The solar composition 3D GCM matches the Spitzer data but generally underpredicts the flux in the WFC3 bandpass and cannot reproduce its featureless shape. This discrepancy could be explained by high atmospheric drag or nightside clouds, and may be better understood through further observation with the James Webb Space Telescope (JWST).Comment: 14 pages, 10 figures, submitted to AAS Journal