117 research outputs found
Social Versus Private Efficiency: A Comparison of Conventional and Organic Farming Systems in Vineyard Production
Organic farming may be seen as an alternative approach to agriculture that tries to integrate environmental concerns in management practices. By means of DEA, in this work we calculate and compare the efficiency of two samples of conventional and organic vineyards, from two different perspectives: in the first instance, the relationship between inputs and outputs is considered, exclusively, that is, the private efficiency; in the second instance, social efficiency is calculated, and the environmental impacts arising from the activity are also included. The comparison of the results obtained in these two scenarios allows us to draw some conclusions on the efficiency of organic farming in dry-farming conditions.organic farming, efficiency, environmental impact, Crop Production/Industries, Farm Management,
Structural basis of the allergenicity to strawberries due to Fra a 1.02
Strawberry fruits are highly valued due to their flavor, aroma, and benefits for human health. Despite this, 30% of the population with food hypersensitivity also shows adverse reactions to strawberry (Franz-Oberdorf et al, 2016). The FaFra a 1 protein family, homologs of the major birch pollen allergen Bet v 1, is involved in this allergenicity to strawberry. By RNAseq we have identified transcripts for 18 members of the FaFra a 1 family (from 1.01 to 1.18) in strawberry fruits. Although expressed in all tissues
analyzed, each family member presents a unique pattern of expression, which suggests functional specialization for each FaFra a 1 protein. FaFra a 1.02 (Fra2 from now on) is the most expressed one in red fruits and is also the most allergenic among the family members tested (Muñoz et al. 2010; Franz- Oberdorf et al, 2016). In order to understand the molecular bases of this allergenicity we crystalized Fra2 and obtained its structure by X-ray diffraction. Fra2 showed a very high structural homology to Bet v 1, and we asked whether the two proteins were recognized by the immune system in a similar way. For this, we generated five different mutant versions of Fra2 in sites described as important for allergenicity in Bet v 1 (Fernandes et al, 2016), and studied their potential allergenicity as well as their crystal structures. Three of the mutants had substitutions in loop 4 (E46R, D48R, E46/48A) and the other two facing the cavity (A141F and Q64W). Compared to Fra2, all the mutants showed a significant reduction in their
capacity to be recognized by the serum of patients with allergies to Bet v 1, and their crystal structures revealed conformational changes in the Bet v 1- IgG interaction sites. Together, these results support that Fra2 and Bet v 1 have similar allergenic determinants We hope this research will aid in understanding how human IgGs interact with Fra2 and might help in the development of new cultivars with a lesser allergenic potential.Grants BIO2013-44199R and
BES-2014-068723 (MINECO). The authors also acknowledge the support by the Plan Propio
from University of Malaga, Campus de Excelencia Internacional de Andalucía
Identifying past social-ecological thresholds to understand long-term temporal dynamics in Spain
A thorough understanding of long-term temporal social-ecological dynamics at the national scale helps to explain the current condition of a country’s ecosystems and to support environmental policies to tackle future sustainability challenges. We aimed to develop a methodological approach to understand past long-term (1960-2010) social-ecological dynamics in Spain. First, we developed a methodical framework that allowed us to explore complex social-ecological dynamics among biodiversity, ecosystem services, human well-being, drivers of change, and institutional responses. Second, we compiled 21 long-term, national-scale indicators and analyzed their temporal relationships through a redundancy analysis. Third, we used a Bayesian change point analysis to detect evidence of past social-ecological thresholds and historical time periods. Our results revealed that Spain has passed through four socialecological thresholds that define five different time periods of nature and society relationships. Finally, we discussed how the proposed methodological approach helps to reinterpret national-level ecosystem indicators through a new conceptual lens to develop a more systems-based way of understanding long-term social-ecological patterns and dynamicsThis work was supported by the Biodiversity Foundation (http://www.fundacion-biodiversidad.es/) of the Spanish Ministry of Agriculture, Food and Environment. Partial
financial support was also provided by the Ministry of Economy and Competitiveness of Spain (project CGL2014-53782-P: ECOGRADIENTES). The Spanish National Institute for
Agriculture and Food Research and Technology (INIA) funded Marina García-Llorente as part of the European Social Fund. Blanca González García-Mon participated in this article as a “la
Caixa” Banking Foundation scholar. The funders had no role in the study design, data collection and analysis, preparation of the report, or the decision to submit the study for publicatio
Do PGI integrated farms perform better? The case of the beef farms in Spain
European rural development policy is gaining in importance through one of its key instruments, the Protected Geographical Indications (PGI) system, which is designed to improve quality standards. Previous research has shown that PGI-certified beef farms tend to be more extensively managed operations that are better adapted to mountainous areas. This paper describes a comparative study of two production systems, one with PGI certification and one without, focusing on a number of economic variables. The results show a positive association between PGI production and profitability. In efficiency terms, non-certified farms show better pure technical efficiency scores, while PGI-certified holdings score higher on scale efficiency
Update on Bone Grafting Materials Used in Dentistry in the Bone Healing Process: Our Experience from Translational Studies to Their Clinical Use
The use of bone grafts is important to preserve the alveolar bone ridge height and volume indispensable for dental implant placement. Despite the highly successful outcomes for the implant-supported overdentures, it seems that a majority of edentulous individuals have not pursued implant-based rehabilitation. Among the reasons cited for this, discrepancy between highly successful therapy and its acceptance is the cost of the treatment. Therefore, the development of biomaterials for bone grafting with comparable characteristics and biological effects than those renowned internationally is necessary. In addition, domestic manufacture would reduce the high costs in public health arising from the application of these biomaterials in the dental field. The aim of the following chapter is to offer an update on one bone grafting material frequently used in dentistry through an assessment of anorganic bovine bone graft in small and medium experimental models as well as its clinical use
More, smaller bacteria in response to ocean's warming?
Heterotrophic bacteria play a major role in organic matter cycling in the ocean. Although the high abundances and relatively fast growth rates of coastal surface bacterioplankton make them suitable sentinels of global change, past analyses have largely overlooked this functional group. Here, time series analysis of a decade of monthly observations in temperate Atlantic coastal waters revealed strong seasonal patterns in the abundance, size and biomass of the ubiquitous flow-cytometric groups of low (LNA) and high nucleic acid (HNA) content bacteria. Over this relatively short period, we also found that bacterioplankton cells were significantly smaller, a trend that is consistent with the hypothesized temperature-driven decrease in body size. Although decadal cell shrinking was observed for both groups, it was only LNA cells that were strongly coherent, with ecological theories linking temperature, abundance and individual size on both the seasonal and interannual scale. We explain this finding because, relative to their HNA counterparts, marine LNA bacteria are less diverse, dominated by members of the SAR11 clade. Temperature manipulation experiments in 2012 confirmed a direct effect of warming on bacterial size. Concurrent with rising temperatures in spring, significant decadal trends of increasing standing stocks (3% per year) accompanied by decreasing mean cell size (-1% per year) suggest a major shift in community structure, with a larger contribution of LNA bacteria to total biomass. The increasing prevalence of these typically oligotrophic taxa may severely impact marine food webs and carbon fluxes by an overall decrease in the efficiency of the biological pump.Versión del editor5,064
What can conservation strategies learn from the ecosystem services approach? Insights from ecosystem assessments in two Spanish protected areas
Biodiversity conservation strategies that overlook the interests of local people are prone to create conflicts. The ecosystem service approach holds potential for more comprehensively integrating the social dimension into decision-making in protected areas, but its implementation in conservation policies is still in its infancy. This research assesses the extent to which ecosystem services have been implemented in conservation strategies in protected areas. The study was conducted in two outstanding Spanish protected areas, covering a wetland (Doñana Natural and National Parks) and a Mediterranean mountain system (Sierra Nevada Natural and National Parks). Data were collected from deliberative workshops with managers and researchers, face-to-face surveys with users and a review of management plans. We found that, beyond intrinsic values of ecosystems and biodiversity, these areas provide multiple ecosystem services that deserve further attention to ensure their sustained delivery. Our research shows that environmental managers and researchers have different perceptions and priorities regarding ecosystem services management compared with ecosystem service users. Environmental managers and researchers in both protected areas perceived that human-nature relationships and ecosystem services are already widely included in management plans, if often not explicitly. We found that different ecosystem service categories receive uneven attention in management plans. These contained measures to manage provisioning and cultural services whereas measures for managing regulating services were perceived to be largely absent. We conclude by summarizing insights on how the ecosystem service approach may enhance the consideration of social interests in the management of management protected areas
Disentangling the complexity of socio-cultural values of temporary rivers
In the last decade, an awareness towards temporary rivers has increased globally in response to drying climates and growing human demand for water. However, social perceptions of
temporary rivers have rarely been incorporated in their science and management. In this study, we advance an understanding of the socio-cultural values of temporary rivers principally
in a European context. We used an ecosystem services-based approach for a participatory and deliberative exercise with 16 researchers and managers. Our results point out to two important aspects of socio-cultural values in temporary rivers. First, cultural ecosystem services have high socio-cultural values and usually represent the interests of the less influential stakeholders in related conflicts. And second, the temporal and geographical variability of these types of rivers is key to understand their socio-cultural values. As an example, the low provision of freshwater in a long non-flowing phase is one of the reasons for its high value. The results above point to future research needs that deserve more attention like the study of tradeoffs and synergies of ecosystem services and interdisciplinary research and management. We finally acknowledge the need to conduct case study research
to account for geographical variation and to include the multiple views of different stakeholder
groups
Transport of Pru p 3 across gastrointestinal epithelium - an essential step towards the induction of food allergy?
Background
Since intestinal absorption of food protein can trigger an allergic reaction, the effect of plant food allergen on intestinal epithelial cell permeability and its ability to cross the epithelial monolayer was evaluated.
Objective
To study the interaction of Pru p 3 with intestinal epithelium, its natural entrance, analyzing transport kinetics and cellular responses that trigger.
Methods
This was achieved using Pru p 3, the peach LTP, as a model. Enterocytic monolayers were established by culturing Caco 2 cells, as a model of enterocytes, on permeable supports that separate the apical and basal compartments. Pru p 3 was added to the apical compartment, the transepithelial resistance (TEER) was measured, and the transport was quantified.
Results
The peach allergen that crossed the cell monolayer was detected in the cell fraction and in the basal medium by immunodetection with specific antibodies and the quantity was measured by ELISA assay. Pru p 3 was able to cross the monolayer without disturbing the integrity of the tight junctions. This transport was significantly higher than that of a non-allergenic peach LTP, LTP1, and occurred via lipid raft pathway. The incubation of Caco 2 cells with Pru p 3 and LTP1 produced the expression of epithelial-specific cytokines TSLP, IL33 and IL25.
Conclusion
These results suggest that Pru p 3 was able to cross the cell monolayer by the transcellular route and then induce the production of Th2 cytokines. The results of the present study represent a step towards clarifying the importance of Pru p 3 as a sensitizer.
Clinical relevance
The capacity of food allergens to cross the intestinal monolayer could explain their high allergenic capacity and its fast diffusion through the body associating to severe symptoms
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