Phytochemical profile and antioxidant capacity of virgin olive oil obtained from the olive cultivar 'Roghiani' from different regions of northern Libya

The aim of this work was to determine the fatty acids (FA), tocopherols, phenols, and antioxidant capacity (AC) of five virgin olive oils (VOO) obtained from the olive cultivar 'Roghiani', from different regions of northern Libya during the crop year 2015. The parameters determined for the oils were investigated for the first time. Seven principal FAs were detected in the VOO samples. Oleic, palmitic, and linoleic acids were the dominant ones. The highest percentage of palmitic acid was found in Tripoli VOO (P lt = 0.05). Oleic acid was dominant in all the VOO samples. Gharyan VOO possessed the highest value of oleic acid, O/L ratio, monounsaturated fatty acids (MUFA), total phenols content (TPC), total tocopherol and tocotrienol contents (TTC), and AC(DPPH)% (P lt = 0.05). A strong positive relationship between TPC and (AC) as (1/EC50DPPH) was observed, (r) was +0.831. On the other hand, (1/EC50DPPH) showed a positive correlation with TTC, (r) was +0.768

Antioxidant Activity of Mulberry Fruit Extracts

Phenolic compounds were extracted from the fruits of Morus nigra and Morus alba using methanol and acetone. The sugar-free extracts (SFEs) were prepared using Amberlite XAD-16 column chromatography. All of the SFEs exhibited antioxidant potential as determined by ABTS (0.75–1.25 mmol Trolox/g), DPPH (2,2-diphenyl-1-picrylhydrazyl) (EC50 from 48 μg/mL to 79 μg/mL), and reducing power assays. However, a stronger activity was noted for the SFEs obtained from Morus nigra fruits. These extracts also possessed the highest contents of total phenolics: 164 mg/g (methanolic SFE) and 173 mg/g (acetonic SFE). The presence of phenolic acids and flavonoids in the extracts was confirmed using HPLC method and chlorogenic acid and rutin were found as the dominant phenolic constituents in the SFEs

Effect of Maturity on Phenolics (Phenolic Acids and Flavonoids) Profile of Strawberry Cultivars and Mulberry Species from Pakistan

In this study, we investigated how the extent of ripeness affects the yield of extract, total phenolics, total flavonoids, individual flavonols and phenolic acids in strawberry and mulberry cultivars from Pakistan. In strawberry, the yield of extract (%), total phenolics (TPC) and total flavonoids (TFC) ranged from 8.5–53.3%, 491–1884 mg gallic acid equivalents (GAE)/100 g DW and 83–327 mg catechin equivalents (CE)/100 g DW, respectively. For the different species of mulberry the yield of extract (%), total phenolics and total flavonoids of 6.9–54.0%, 201–2287 mg GAE/100 g DW and 110–1021 mg CE/100 g DW, respectively, varied significantly as fruit maturity progressed. The amounts of individual flavonols and phenolic acid in selected berry fruits were analyzed by RP-HPLC. Among the flavonols, the content of myricetin was found to be high in Morus alba (88 mg/100 g DW), the amount of quercetin as high in Morus laevigata (145 mg/100 g DW) while kaempferol was highest in the Korona strawberry (98 mg/100 g DW) at fully ripened stage. Of the six phenolic acids detected, p-hydroxybenzoic and p-coumaric acid were the major compounds in the strawberry. M. laevigata and M. nigra contained p-coumaric acid and vanillic acid while M. macroura and M. alba contained p-hydroxy-benzoic acid and chlorogenic acid as the major phenolic acids. Overall, a trend to an increase in the percentage of extraction yield, TPC, TFC, flavonols and phenolic acids was observed as maturity progressed from un-ripened to fully-ripened stages

Application of phenolic extracts from selected plants in fruit juice

Plant phenolics have gained considerable interest in recent years for their potential effects against food related microorganisms. In the present study, phenolic extracts from the leaves of three plants namely Moringa oleifera, Morus indica, and Mentha spicata were prepared by a mixture of methanol/acetone/water. The UV spectra of extracts were recorded and contents of total phenolics determined. The extracts were incorporated in pineapple juice and their solubility and stability were studied. In addition, the acceptability of treated juice samples was sensory evaluated. The solubility of M. indica and M. oleifera extracts was found to be more than that of M. spicata. The two extracts were also stable in the pH environment of pineapple juice when stored at 4°C for two weeks. The pineapple juice treated with M. indica extract was more acceptable compared to that of M. oleifera, according to sensory evaluation. Therefore, the effect of addition of phenolic extract from M. indica on the shelf life of pineapple juice stored at 4°C was investigated by monitoring the changes in titrable acidity and sensory parameters for 8 weeks. Results indicated that the extracts of natural phenolic compounds can be used to improve the quality and safety of foods

Antioxidant properties of various solvent extracts of mulberry (Morus indica L.) leaves

The antioxidant properties and total phenolic contents of methanol, acetone and water extracts of mulberry (Morus indica L.) leaves were examined. Various experimental models including iron (III) reducing capacity, total antioxidant capacity, DPPH radical scavenging activity and in vitro inhibition of ferrous sulphate-induced oxidation of lipid system were used for characterization of antioxidant activity of extracts. The three extracts showed varying degrees of efficacy in each assay in a dose-dependent manner. Methanolic extract with the highest amount of total phenolics, was the most potent antioxidant in all the assays used. In addition, the effect of temperature (50°C and 100°C), pH (3, 5, 7, 9 and 11) and storage (5°C) on the antioxidant activity of methanolic extract was investigated. The antioxidant activity of the extract remained unchanged at 50°C and was maximum at neutral pH. The extract stored at 5°C in the dark was stable for 30 days after which the antioxidant activity decreased (p⩽0.05) gradually. On the basis of the results obtained, mulberry leaves were found to serve as a potential source of natural antioxidants due to their marked antioxidant activity

Corresponding Author Antioxidant Properties of Various Solvent Extracts of Lemon Verbena (Lippia Citriodora) Leaves

Abstract Lemon verbena (Lippia citriodora) belongs tothe Verbenaceae (shahpasand) family. L.citriodora is chemical composition of the leaves plant have antimicrobial properties, refrigerant, anti-headaches onesided, anti-pains nerve and housing, carminative, helping to digestion, relaxation, dizziness, colds therapy, memory Booster and etc. In this study, phenolic compounds of L.citriodora leaves were extracted with immersion method by acetone, ethanol, methanol and water. In this method, solvent type was found as an effective factor on the extraction. The highest amount of total phenol compounds was extracted by methanol which was 25/94 (mg Gallic acid per 100 mg of the extract). Among the solvents,acetone extremely had lower efficiency in the extracting phenolic compounds from the leaves of plant. Three complementary assays, reducing power of Fe (III), DPPH radical scavenging and total antioxidant capacity were used for analysis of antioxidant properties of various extracts compared with the synthetic antioxidant, BHT. In all tests, methanolic extract, which had the highest total phenolic compounds, showed the highest antioxidant activity although it was lower that of BHT. According to the survey results, L.citriodora leaves with a good source of natural antioxidants for use in food industry's

Antioxidant efficacy of mulberry (Morus Indica L.) leaves extract and powder in edible oil

The antioxidant activity of mulberry (Morus Indica L.) leaves was evaluated in rice bran oil (RBO). The oil was subjected to accelerated oxidation at 100 degrees C for 5 days and heat treatment at 180 degrees C for 1 h. In order to examine its potential antioxidant activity, the oxidative stability of the oils was evaluated by employing peroxide value (PV), radical scavenging activity (RSA), thiobarbituric acid reactive substances assay (TBARS), and percent free fatty acids (FFA). Mulberry in two forms viz: the methanolic extract and powder were applied to RBO at 200 ppm and 0.05%, respectively, and results were compared with RBO treated with a synthetic antioxidant-BHT. The PV, RSA, TBARS, and FFA values indicated that both mulberry extract and powder were effective in inhibiting lipid oxidation when compared to synthetic antioxidant BHT. Thus, mulberry leaves may serve as a new source of natural antioxidant for use in food systems