Structural Characterization and Physical Properties of Syzygium cumini Flowering Plant

ABSTRACT: In this study, the flowering plant of syzygium cumini was used. X-ray fluorescence (XRF) and X-ray diffraction (XRD) were used to investigate the structure of the leaves and barks of syzygium cumini. The XRD results were indicated that the structures of both leaves and bark were amorphous. The XRF results indicated that both leaves and barks contained Potassium (K), Calcium (Ca), (Ti), and Magnesium (Mn), Iron (Fe), Copper (Cu), Zinc (Zn), Zernike (As), Lead (Pb), Barium (Br), Strontium (Sr). The optical properties of syzygium cumini were carried out using Fourier Transformation infrared spectroscopy (FTIR) and Ultra violet spectroscopy (UV). The FTIR spectra showed a broad and strong absorption band in the range (685-1638) cm -1 , and these absorptions were assigned to the different stretching vibrations. The absorption for bark and leave was found to be 2.45 and 2.52 a u, while the wave length was found to be 277.4 and 277.5 nm, respectively. The energy band gap is calculated and found to be 5.017 and 4.67 eV, for bark and leave, respectively

Copy number rather than epigenetic alterations are the major dictator of imprinted methylation in tumors

It has been postulated that imprinting aberrations are common in tumors. To understand the role of imprinting in cancer, we have characterized copy-number and methylation in over 280 cancer cell lines and confirm our observations in primary tumors. Imprinted differentially methylated regions (DMRs) regulate parent-of-origin monoallelic expression of neighboring transcripts in cis. Unlike single-copy CpG islands that may be prone to hypermethylation, imprinted DMRs can either loose or gain methylation during tumorigenesis. Here, we show that methylation profiles at imprinted DMRs often not represent genuine epigenetic changes but simply the accumulation of underlying copy-number aberrations (CNAs), which is independent of the genome methylation state inferred from cancer susceptible loci. Our results reveal that CNAs also influence allelic expression as loci with copy-number neutral loss-of-heterozygosity or amplifications may be expressed from the appropriate parental chromosomes, which is indicative of maintained imprinting, although not observed as a single expression foci by RNA FISH