DNA Barcoding and Phylogeny of Patellids from Asturias (Northern Spain)

The main role for genetics in marine invertebrates is the identification of species and groups of interbreeding individuals. In Asturias exists an ancient culinary tradition of consumption for limpets (Patella s.s.) but there is a lack of studies about these species. We have sampled Asturian marine Patella s.s. specimens and conducted sequencing of the mtDNA COI gene. We have confirmed the presence of four Patella s.s. in the Asturian coasts (P. vulgata, P. depressa, P.aspera, and P. rustica). Our work raises concerns about the current state of the P. vulgata populations in Asturias, where it is exploited, due to its low levels of genetic variation. Our phylogenetic analyses using Bayesian approaches confirmed that patellids belong to four different clades but gives a new version about how these clades are related inside the genus aiming for the necessity of more work to address this issue

Evolution of growth rates in triploid and diploid turbot Scophthalmus maximus (L., 1758)

El crecimiento de los peces triploides de diversas especies ha sido objetivo de múltiples trabajos cuyos resultados no indican una pauta sostenida de comportamiento en cuanto a esta variable, obteniéndose a menudo resultados contradictorios, incluso entre individuos de la misma especie. En este trabajo se analizan las tasas de crecimiento de dos grupos de individuos triploides de rodaballo Scophthalmus maximus (L., 1758) producidos mediante la técnica de choques térmicos fríos en distintos momentos y procedentes de diferentes stocks reproductores. Los datos indican que no hay diferencias entre los diploides y los triploides y, además, que las similitudes son mayores entre cada grupo y su control que entre los grupos del mismo[Abstract] The literature on the growth rates of triploid fish before reaching maturity is inconclusive, often reporting contradictory results, even between individuals of the same species. The present paper analyses the growth rate of two groups of diploid and triploid turbot Scophthalmus maximus (L., 1758). The triploids were produced through the technique of thermal cold shocks. The specimens originated from two different broodstocks. No differencesInstituto Español de Oceanografí

Development and Evaluation of a Disease Large Animal Model for Preclinical Assessment of Renal Denervation Therapies

[EN] New-generation catheters-based renal denervation (RDN) is under investigation for the treatment of uncontrolled hypertension (HTN). We assessed the feasibility of a large animal model of HTN to accommodate the human RDN devices. Ten minipigs were instrumented to measure blood pressure (BP) in an awake-state. HTN was induced with subcutaneous 11-deoxycorticosterone (DOCA, 100 mg/kg) implants. Five months after, the surviving animals underwent RDN with the Symplicity® system. Norepinephrine (NE) renal gradients were determined before and 1 month after RDN. Renal arteries were processed for histological (hematoxylin-eosin, Movat pentachrome) and immunohistochemical (S100, tyrosine-hydroxylase) analyses. BP significantly rose after DOCA implants. Six animals died prematurely, mainly from infectious causes. The surviving animals showed stable BP levels after 5 months. One month after RDN, nerve damage was showed in three animals, with impedance drop >10%, NE gradient drop and reduction in BP. The fourth animal showed no nerve damage, impedance drop <10%, NE gradient increase and no change in BP. In conclusion, the minipig model of DOCA-induced HTN is feasible, showing durable effects. High mortality should be addressed in next iterations of this model. RDN may partially offset the DOCA-induced HTN. Impedance drop and NE renal gradient could be markers of RDN success.SIThis research was funded by Consejería de Salud, Junta de Castilla and Leon, Spain, the Grant GRS 1001/A/2014.We want to thank Medtronic Iberia for the donation of the Symplicity catheters used in this experiment

Aprender a enseñar creando recursos digitales multimedia

Virtual USATIC 2017En el Grado de Maestro en Educación Primaria una de las asignaturas que ofrece formación para aprender a enseñar utilizando las tecnologías emergentes es "las Tecnologías de la Información y Comunicación aplicadas a la Educación Primaria". Este año mediante un proceso de aprendizaje colaborativo se propuso ofrecer a los estudiantes las herramientas necesarias para llevar a cabo de forma efectiva la creación de recursos digitales multimedia. Los recursos digitales multimedia facilitan la comprensión de la información y son un recurso de apoyo muy interesante para la docencia. La combinación acertada de imágenes (fijas y/o móviles) y texto multiplica el poder de comunicación del docente. La eficacia didáctica de tales recursos está relacionada con el diseño profesional de las mismas. En esta comunicación se dan a conocer diversos recursos digiitales multimedia creados por el propio alumnado, futuro docente

Subscapular and triceps skinfolds reference values of Hispanic American children and adolescents and their comparison with the reference of Centers for Disease Control and Prevention (CDC)

Introduction: the assessment of the skinfold thickness is an objective measure of adiposity. Therefore, it is a useful tool for nutritional diagnosis and prevention of metabolic risk associated with excess fat in chilhood and adolescence. Objective: to provide percentiles of subscapular and triceps skinfolds for Hispanic American schoolchildren and compare them with those published by the Centers for Disease Control and Prevention (CDC) from United States, that it have been commonly used as a reference in most of these countries. Methods: subscapular and triceps skinfolds were measured in 9.973 schoolchildren 4-19 aged from Spain, Argentina, Cuba, Venezuela and Mexico with Holtain caliper with 0.2 mm accuracy. Percentiles were obtained with the LMS statistical method and were presented in tables divided in stages of 6 months and in curves graphics. The difference between Hispanic American and CDC mean values were provided for P3, P50 and P97 in mm and also were graphically represented. Results: skinfolds measurements obviously increased with age in both sexes but, in boys, this increase is much more marked in highest percentiles between 8 and 13 years; this maximum is reached earlier than what occurs in CDC reference. In both sexes, all percentiles analized in Hispanic American schoolchildren were higher than the CDC reference except P97 up to 10 or 13 years that was notably smaller. Conclusions: the skinfolds percentiles of Hispanic American children and adolescents differ from CDC that are usually used as reference. The values of subscapular and triceps skinfolds provided in this study, could be applied to populations of a similar ethnic background, especially in comparative studies of body composition

Redes docentes interdisciplinares para promover la infoaccesibilidad en campus virtuales inclusivos

Jornadas de Redes de Investigación en Innovación Docente de la UNED (9º. 2017. Madrid)Con el objetivo de promover la infoaccesibilidad para la creación de campus virtuales inclusivos en la Educación Superior, potenciando a la vez el trabajo cooperativo y colaborativo del profesorado universitario, se constituyó una red docente entre especialistas de distintas disciplinas que imparten docencia en la misma Facultad pero en distintas titulaciones (Grado de Maestro en Educación Primaria, Grado de Maestro en Educación Infantil y Grado en Pedagogía). La propuesta se estructuró en forma de un proyecto de innovación docente desarrollado en la Universidad de Oviedo, mediante la creación de un nuevo grupo de trabajo que implicó al Departamento de Ciencias de la Educación y a varias áreas de conocimiento (Área de Didáctica de Ciencias Experimentales, Área de Didáctica y Organización Escolar y Área de Métodos de Investigación y Diagnóstico en Educación ) para atender a la diversidad del alumnado, fomentando el aprendizaje mixto o blended learning en el campus virtual. Se utilizó como herramienta de diagnóstico una encuesta previa a los estudiantes matriculados en las asignaturas implicadas para recabar sus experiencias, percepciones y necesidades. Mediante la aplicación de diversas herramientas tecnológicas para promover la infoaccesibilidad, los resultados de este proyecto se han podido extender a 11 asignaturas que implican aproximadamente a 500 alumnos matriculados. Entre las repercusiones de este proyecto de innovación se destaca la de explorar el uso de los recursos tecnológicos disponibles desde la perspectiva de la accesibilidad y multimodalidad para su difusión entre el profesorado, promoviendo en la mayor medida posible la infoaccesibilidad en nuestros campus virtuales universitarios

Construyendo Campus Virtuales Inclusivos: la Infoaccesibilidad como Estrategia de Innovación (PAINN16-067)

Jornadas de Innovación Docente de la Universidad de Oviedo (10ª. 2017)La Universidad de Oviedo ha extendido en los últimos años las capacidades y usos del Campus Virtual (plataforma de aprendizaje basada en Moodle) que posibilita tanto el aprendizaje mixto (blended-learning) como la educación a distancia en línea (e-learning) dentro de las titulaciones y cursos ofertados. Esto abre un nuevo abanico de metodologías y recursos didácticos para facilitar el aprendizaje, con nuevas posibilidades y desafíos a la hora de llegar efectivamente a todo el alumnado. Con el objetivo de atender a la diversidad del alumnado y promover la infoaccesibilidad para la creación de campus virtuales inclusivos en la Educación Superior, se generó un grupo de trabajo entre especialistas de distintas disciplinas que imparten docencia en las titulaciones ofertadas por la Facultad de Formación del Profesorado y Educación. El proyecto se organizó en varias fases: 1) generación de la red docente y valoraciones previas del profesorado, 2) diagnóstico mediante encuesta previa al alumnado; 3) aplicación de herramientas tecnológicas para promover la infoaccesibilidad en las distintas asignaturas. Los resultados de este proyecto se han extendido a 11 asignaturas que implican aproximadamente a 500 personas matriculadas. Entre las repercusiones de este proyecto de innovación se destaca la de explorar el uso de los recursos tecnológicos disponibles desde la perspectiva de la accesibilidad y multimodalidad para su difusión entre el profesorado, promoviendo en la mayor medida posible la infoaccesibilidad en nuestros campus virtuales universitarios

Genetic diversity of HLA system in four populations from Baja California, Mexico: Mexicali, La Paz, Tijuana and rural Baja California

We studied HLA class I (HLA-A, -B) and class II (HLA-DRB1, -DQB1) alleles by PCR-SSP based typing in 250 Mexicans from the states of Baja California Norte and Baja California Sur living in Mexicali (N = 100), La Paz (N = 75), Tijuana (N = 25) and rural communities (N = 50) to obtain information regarding allelic and haplotypic frequencies. The most frequent haplotypes for the Baja California region include nine Native American and five European haplotypes. Admixture estimates revealed that the main genetic components are European (50.45 ± 1.84% by ML; 42.03% of European haplotypes) and Native American (43.72 ± 2.36% by ML; 40.24% of Native American haplotypes), while the African genetic component was less apparent (5.83 ± 0.98% by ML; 9.36% of African haplotypes)

Wide-Geographic and Long-Term Analysis of the Role of Pathogens in the Decline of Pinna nobilis to Critically Endangered Species

A mass mortality event (MME) affecting the fan mussel Pinna nobilis was first detected in Spain in autumn 2016 and spread north- and eastward through the Mediterranean Sea. Various pathogens have been blamed for contributing to the MME, with emphasis in Haplosporidium pinnae, Mycobacterium sp. and Vibrio spp. In this study, samples from 762 fan mussels (necropsies from 263 individuals, mantle biopsies from 499) of various health conditions, with wide geographic and age range, taken before and during the MME spread from various environments along Mediterranean Sea, were used to assess the role of pathogens in the MME. The number of samples processed by both histological and molecular methods was 83. The most important factor playing a main role on the onset of the mass mortality of P. nobilis throughout the Mediterranean Sea was the infection by H. pinnae. It was the only non-detected pathogen before the MME while, during MME spreading, its prevalence was higher in sick and dead individuals than in asymptomatic ones, in MME-affected areas than in non-affected sites, and it was not associated with host size, infecting both juveniles and adults. Conversely, infection with mycobacteria was independent from the period (before or during MME), from the affection of the area by MME and from the host health condition, and it was associated with host size. Gram (-) bacteria neither appeared associated with MME.En prens

Strategies for GHG mitigation in Mediterranean cropping systems. A review

In this review we aimed to synthetize and analyze the most promising GHGs mitigation strategies for Mediterranean cropping systems. A description of most relevant measures, based on the best crop choice and management by farmers (i.e., agronomical practices), was firstly carried out. Many of these measures can be also efficient in other climatic regions, but here we provide particular results and discussion of their efficiencies for Mediterranean cropping systems. An integrated assessment of management practices on mitigating each component of the global warming potential (N2O and CH4 emissions and C sequestration) of production systems considering potential side-effects of their implementation allowed us to propose the best strategies to abate GHG emissions, while sustaining crop yields and mitigating other sources of environmental pollution (e.g. nitrate leaching and ammonia volatilization)