Morphological changes, beach inundation and overwash caused by an extreme storm on a low-lying embayed beach bounded by a dune system (NW Mediterranean)

The geomorphological evolution of a low-lying, micro-tidal sandy beach in the western Mediterranean, Pals beach, was characterized using airborne Light Detection and Ranging (LiDAR) data. Data were collected in prior to and six months after the impact of an extreme storm with a return period of approx. 50 years, with the aim of characterizing the beach's response to the storm. The use of repeated high-resolution topographic data to quantify beach geomorphic changes has allowed assessment of the accuracy of different proxies for estimating beach volume changes. Results revealed that changes in the shoreline position cannot accurately reproduce beach volume changes on low-lying beaches where overwash processes are significant. Observations also suggested that volume estimations from beach profiles do not accurately represent subaerial volume changes at large profile distances on beaches with significant alongshore geomorphological variability. Accordingly, the segmentation of the beach into regularly spaced bins is proposed to assess alongshore variations in the beach volume with the accuracy of the topographic data. The morphological evolution of Pals beach during the study period showed a net shoreline retreat (- 4 m) and a significant sediment gain on the subaerial beach (+ 7.5 m3/m). The net gain of sediment is mostly due to the impact of the extreme storm, driving significant overwash processes that transport sediment landwards, increasing volume on the backshore and dunes. The increase of volume on the foreshore and the presence of cuspate morphologies along the shoreline also evidence post-storm beach recovery. Observed morphological changes exhibit a high variability along the beach related to variations in beach morphology. Changes in the morphology and migration of megacusps result in a high variability in the shoreline position and foreshore volume changes. On the other hand, larger morphological changes on the backshore and larger inundation distances occur when the beach and the dunes are lower, favouring the dominance of overwash. The observed storm-induced morphological changes differ from predicted beach storm impacts because of spatial and temporal variations in the beach morphology, suggesting that detailed morphological parameters and indicators used for predicting beach vulnerability to storms should be regularly updated in order to represent the pre-storm beach conditions. Finally, observed morphological changes in Pals Bay evidenced a different behaviour between natural and urban areas, with better post-storm beach recovery on natural areas where the beach is not artificially narrowed.Peer ReviewedPostprint (author's final draft

Efecto del asesoramiento nutricional sobre la ingesta de nutrientes relacionados con la mineralización ósea en gimnastas de artística femenina = Effect of nutritional advice on nutrient ingestion related to bone mineralization in female artistic gymnasts

Las gimnastas de artística femenina de alto nivel dedican una gran parte del día a los entrenamientos y residen desde edades muy tempranas fuera del entorno familiar, alcanzando su máximo rendimiento durante la adolescencia dónde la nutrición desempeña un papel primordial. Se valoró la dieta de la selección nacional de gimnasia artística en dos periodos de 5 días cada uno, uno sin asesoramiento y otro tras asesoramiento (educación nutricional y diseño de nuevas dietas), dentro del mismo periodo de entrenamiento (precompetitivo). Se utilizó el método de registro de pesada de todos los alimentos consumido y se estimó su gasto energético en los dos mismos periodos de cinco días. El asesoramiento nutricional condujo a mejoras en la composición de la dieta de las gimnastas así como a aumentos en el consumo de gran parte de vitaminas y minerales. El asesoramiento nutricional a largo plazo podría resultar beneficioso para la salud de las gimnastas así como para su rendimiento deportivo. Competitive female artistic gymnasts dedicate a great deal of their day to training and live outside their family enviroment from a very young age, these althletes reach maximum performance during adolescence, a time in which nutrition plays a crucial role. We assessed food intake of the National Team for two 5 day periods, without and with nutritional counselling (nutritional education and new diets desing), in the same training period (precompetitive period). Diet composition was estimated by food weighing and, during the same days, we estimated their energy expenditure. Nutritional counselling resulted in improvements on the diet composition of the gymnasts as well as increases on the vitamins and minerals intake in most of them. Long term nutritional counselling can provide beneficial results for the gymnast’s health and athletic performance

SUMOylation regulates LKB1 localization and its oncogenic activity in liver cancer

BACKGROUND: Even though liver kinase B1 (LKB1) is usually described as a tumor suppressor in a wide variety of tissues, it has been shown that LKB1 aberrant expression is associated with bad prognosis in Hepatocellular Carcinoma (HCC). METHODS: Herein we have overexpressed LKB1 in human hepatoma cells and by using histidine pull-down assay we have investigated the role of the hypoxia-related post-translational modification of Small Ubiquitin-related Modifier (SUMO)ylation in the regulation of LKB1 oncogenic role. Molecular modelling between LKB1 and its interactors, involved in regulation of LKB1 nucleocytoplasmic shuttling and LKB1 activity, was performed. Finally, high affinity SUMO binding entities-based technology were used to validate our findings in a pre-clinical mouse model and in clinical HCC. FINDINGS: We found that in human hepatoma cells under hypoxic stress, LKB1 overexpression increases cell viability and aggressiveness in association with changes in LKB1 cellular localization. Moreover, by using site-directed mutagenesis, we have shown that LKB1 is SUMOylated by SUMO-2 at Lys178 hampering LKB1 nucleocytoplasmic shuttling and fueling hepatoma cell growth. Molecular modelling of SUMO modified LKB1 further confirmed steric impedance between SUMOylated LKB1 and the STe20-Related ADaptor cofactor (STRADα), involved in LKB1 export from the nucleus. Finally, we provide evidence that endogenous LKB1 is modified by SUMO in pre-clinical mouse models of HCC and clinical HCC, where LKB1 SUMOylation is higher in fast growing tumors. INTERPRETATION: Overall, SUMO-2 modification of LKB1 at Lys178 mediates LKB1 cellular localization and its oncogenic role in liver cancer. FUND: This work was supported by grants from NIH (US Department of Health and Human services)-R01AR001576-11A1 (J.M.M and M.L.M-C.), Gobierno Vasco-Departamento de Salud 2013111114 (to M.L.M.-C), ELKARTEK 2016, Departamento de Industria del Gobierno Vasco (to M.L.M.-C), MINECO: SAF2017-87301-R and SAF2014-52097-R integrado en el Plan Estatal de Investigación Cientifica y Técnica y Innovación 2013-2016 cofinanciado con Fondos FEDER (to M.L.M.-C and J.M.M., respectively), BFU2015-71017/BMC MINECO/FEDER, EU (to A.D.Q. and I.D.M.), BIOEF (Basque Foundation for Innovation and Health Research): EITB Maratoia BIO15/CA/014; Instituto de Salud Carlos III:PIE14/00031, integrado en el Plan Estatal de Investigación Cientifica y Técnica y Innovacion 2013-2016 cofinanciado con Fondos FEDER (to M.L.M.-C and J.M.M), Asociación Española contra el Cáncer (T.C.D, P·F-T and M.L.M-C), Daniel Alagille award from EASL (to T.C.D), Fundación Científica de la Asociación Española Contra el Cancer (AECC Scientific Foundation) Rare Tumor Calls 2017 (to M.L.M and M.A), La Caixa Foundation Program (to M.L.M), Programma di Ricerca Regione-Università 2007-2009 and 2011-2012, Regione Emilia-Romagna (to E.V.), Ramón Areces Foundation and the Andalusian Government (BIO-198) (A.D.Q. and I.D.M.), ayudas para apoyar grupos de investigación del sistema Universitario Vasco IT971-16 (P.A.), MINECO:SAF2015-64352-R (P.A.), Institut National du Cancer, FRANCE, INCa grant PLBIO16-251 (M.S.R.), MINECO - BFU2016-76872-R to (E.B.). Work produced with the support of a 2017 Leonardo Grant for Researchers and Cultural Creators, BBVA Foundation (M.V-R). Finally, Ciberehd_ISCIII_MINECO is funded by the Instituto de Salud Carlos III. We thank MINECO for the Severo Ochoa Excellence Accreditation to CIC bioGUNE (SEV-2016-0644). Funding sources had no involvement in study design; in the collection, analysis, and interpretation of data; in the writing of the report; and in the decision to submit the paper for publication

Impacts of sea-level rise-induced erosion on the Catalan coast

The final publication is available at Springer via http://dx.doi.org/10.1007/s10113-016-1052-xThe Catalan coast as most of the developed Mediterranean coastal zone is characterized by the coincidence of stresses and pressures on the natural system with a high exposure and low adaptive capacity. Due to this, climate change-induced effects will increase natural hazards and aggravate their associated impacts and, in consequence, it is necessary to assess their effects for proper long-term management. In this work, we assess the impact of sea-level rise (SLR)-induced shoreline retreat on the Catalan coast for three scenarios ranging from 0.53 to 1.75 m by the year 2100. Implications are analysed in terms of affectation of two main functions provided by beaches, i.e. recreation and protection. Obtained results show that CC will be a serious threat to analysed functions since the expected enhanced shoreline retreat will severely decrease the recreational carrying capacity and the capacity of protection in the near future under tested scenarios. The actual level of development along the coastal zone reduces the natural resilient capacity of beaches to SLR in such a way that the lack of accommodation space can be identified as a main factor for the estimated impacts.Peer ReviewedPostprint (author's final draft

Dimerization model of the C-terminal RNA Recognition Motif of HuR

In PressHuman antigen R (HuR) is a ubiquitous 32kDa protein comprising three RNA Recognition Motifs (RRMs), whose main function is to bind Adenylate and uridylate Rich Elements (AREs) in 3′ UnTranslated Regions (UTRs) of mRNAs. In addition to binding RNA molecules, the third domain (RRM3) is involved in HuR oligomerization and apoptotic signaling. The RRM3 monomer is able to dimerize, with its self-binding affinity being dependent on ionic strength. Here we provide a deeper structural insight into the nature of the encounter complexes leading to the formation of RRM3 dimers by using Brownian Dynamics and Molecular Dynamics. Our computational data show that the initial unspecific encounter follows a downhill pathway until reaching an optimum conformation stabilized by hydrophobic interactions.I.D.-M. wishes to thank the Andalusian Government (P07-CVI-02896, P11-CVI-07216 and BIO198) for financial support.Peer reviewe

Cytochrome c signalosome in mitochondria

Cytochrome c delicately tilts the balance between cell life (respiration) and cell death (apoptosis). Whereas cell life is governed by transient electron transfer interactions of cytochrome c inside the mitochondria, the cytoplasmic adducts of cytochrome c that lead to cell death are amazingly stable. Interestingly, the contacts of cytochrome c with its counterparts shift from the area surrounding the heme crevice for the redox complexes to the opposite molecule side when the electron flow is not necessary. The cytochrome c signalosome shows a higher level of regulation by post-translational modifications—nitration and phosphorylation—of the hemeprotein. Understanding protein interfaces, as well as protein modifications, would puzzle the mitochondrial cytochrome c-controlled pathways out and enable the design of novel drugs to silence the action of pro-survival and pro-apoptotic partners of cytochrome c.Spanish Ministry of Science and Innovation BFU2009-07190Andalusian Government BIO198 P08-CVI-387

Shaping of 3YSZ porous substrates for oxygen separation membranes

[EN] In recent years, asymmetric membranes based on mixed ionic-electronic conductors (MIEC) have gained importance in practical gas separations. MIEC ceramic materials show high-energy efficiency and high temperature resistance, which allows direct integration in industrial processes. Thin layers are supported on porous substrates that provide mechanical strength. In the asymmetric membrane manufacture, the control of support porosity and microstructure is crucial. Colloidal processing is an interesting method that allows controlling the final microstructure in both surfaces and bulk, with high reproducibility. Here, the development of asymmetric membranes with a top functional layer made of Ce0.8Gd0.2O1.9/Ni2FeO4 composite is presented and aims to maximize oxygen permeation and membrane robustness. The porous substrate is prepared by slip casting while the functional layers by screen-printing. The effect of pore former volume and particle morphology were studied. The combination of spherical and flake-like PMMA particles enabled to generate open porosity suitable for fast gas transport.Financial funding from the Spanish Government (ENE2014-57651 and SEV-2012-0267 grants) is gratefully acknowledged.Escribano-Quintana, JA.; García-Fayos, J.; Serra Alfaro, JM. (2017). Shaping of 3YSZ porous substrates for oxygen separation membranes. Journal of the European Ceramic Society. 37(16):5223-5231. https://doi.org/10.1016/j.jeurceramsoc.2017.05.032S52235231371

Analysis of the rear leg rotation movement during the fencing lunge

The most relevant aulhois of fencing agree on the extension of the rear leg's knee from the on guard position, which is the ona thai provides ihe real speed to the lunge, and the extension of the arm only serves to drive the points towards the target. All the authors coincide thai it is important to keep the whole sole of the rear Foot supported on the Boor, keeping the same direction and without sliding it during the movernent, being the only one support that assures a really fast and balanced lunge, allowing the return to the on guard position or to continue towards ahead. Nevertheless, in a competition it may be observed that there are just a few fencers that keep the position of the feet in 90° angle during the lunge, as proposed in the fencing books. From this situation, we try to know if the rotation movement of the rear leg improve the speed of the Mass Centre and the weapon

Teaching Competencies in Nursing Professors: Visions of Students and Academics

Objective. This work sought to know the view of Nursing professors and students about the competencies the faculty staff must have to deploy their educational function with maximum quality and efficiency. Methods. Descriptive qualitative study through focus groups conducted with professors, students and recent Nursing career graduates from universities in Spain. Results. The importance of the proposed teaching competencies was delved into, highlighting the importance of professors knowing the context in which they teach, having the ability to selfevaluate their activity, and having adequate interpersonal communication skills, and deploy the teaching-learning process by performing proper planning, using new technologies, and knowing how to engage in teamwork. Moreover, a small discrepancy was detected in relation to disciplinary competence, which students felt was of importance, but which academics indicated is taken for granted in nursing professors; competencies directly related to the act of teaching must be enhanced. Conclusion. Practical unanimity was found between academics and students in affirming that the competencies investigatedare important for adequate development of the teaching activity in nursing professors. In all cases, the urgent need was highlighted for nursing professors to have adequate teaching training to provide their students with formation of the highest quality