Brazilian consortium for the study on renal diseases associated with COVID-19 : a multicentric effort to understand SARS-CoV-2-related nephropathy

Kidney involvement appears to be frequent in coronavirus disease 2019 (COVID-19). Despite this, information concerning renal involvement in COVID-19 is still scarce. Several mechanisms appear to be involved in the complex relationship between the virus and the kidney. Also, different morphological patterns have been described in the kidneys of patients with COVID-19. For some authors, however, this association may be just a coincidence. To investigate this issue, we propose assessing renal morphology associated with COVID-19 at the renal pathology reference center of federal university hospitals in Brazil. Data will come from a consortium involving 17 federal university hospitals belonging to Empresa Brasileira de Serviços Hospitalares (EBSERH) network, as well as some state hospitals and an autopsy center. All biopsies will be sent to the referral center for renal pathology of the EBSERH network. The data will include patients who had coronavirus disease, both alive and deceased, with or without pre-existing kidney disease. Kidney biopsies will be analyzed by light, fluorescence, and electron microscopy. Furthermore, immunohistochemical (IHC) staining for various inflammatory cells (i.e., cells expressing CD3, CD20, CD4, CD8, CD138, CD68, and CD57) as well as angiotensin-converting enzyme 2 (ACE2) will be performed on paraffinized tissue sections. In addition to ultrastructural assays, in situ hybridization (ISH), IHC and reverse transcription-polymerase chain reaction (RT-PCR) will be used to detect Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV-2) in renal tissue. For the patients diagnosed with Collapsing Glomerulopathy, peripheral blood will be collected for apolipoprotein L-1 (APOL1) genotyping. For patients with thrombotic microangiopathy, thrombospondin type 1 motif, member 13 (ADAMTS13), antiphospholipid, and complement panel will be performed. The setting of this study is Brazil, which is second behind the United States in highest confirmed cases and deaths. With this complete approach, we hope to help define the spectrum and impact, whether immediate or long-term, of kidney injury caused by SARS-CoV-2

ATLANTIC-PRIMATES: a dataset of communities and occurrences of primates in the Atlantic Forests of South America

Primates play an important role in ecosystem functioning and offer critical insights into human evolution, biology, behavior, and emerging infectious diseases. There are 26 primate species in the Atlantic Forests of South America, 19 of them endemic. We compiled a dataset of 5,472 georeferenced locations of 26 native and 1 introduced primate species, as hybrids in the genera Callithrix and Alouatta. The dataset includes 700 primate communities, 8,121 single species occurrences and 714 estimates of primate population sizes, covering most natural forest types of the tropical and subtropical Atlantic Forest of Brazil, Paraguay and Argentina and some other biomes. On average, primate communities of the Atlantic Forest harbor 2 ± 1 species (range = 1–6). However, about 40% of primate communities contain only one species. Alouatta guariba (N = 2,188 records) and Sapajus nigritus (N = 1,127) were the species with the most records. Callicebus barbarabrownae (N = 35), Leontopithecus caissara (N = 38), and Sapajus libidinosus (N = 41) were the species with the least records. Recorded primate densities varied from 0.004 individuals/km 2 (Alouatta guariba at Fragmento do Bugre, Paraná, Brazil) to 400 individuals/km 2 (Alouatta caraya in Santiago, Rio Grande do Sul, Brazil). Our dataset reflects disparity between the numerous primate census conducted in the Atlantic Forest, in contrast to the scarcity of estimates of population sizes and densities. With these data, researchers can develop different macroecological and regional level studies, focusing on communities, populations, species co-occurrence and distribution patterns. Moreover, the data can also be used to assess the consequences of fragmentation, defaunation, and disease outbreaks on different ecological processes, such as trophic cascades, species invasion or extinction, and community dynamics. There are no copyright restrictions. Please cite this Data Paper when the data are used in publications. We also request that researchers and teachers inform us of how they are using the data. © 2018 by the The Authors. Ecology © 2018 The Ecological Society of Americ

Uretrostomia pré-púbica videoassistida em um felino com estenose uretral

Urethrostomy is a surgical technique used in cases of partial or total obstruction of the urethra, and the laparoscopic approach may be a treatment option. Due to the scarcity of studies involving the technique, this paper reports the case of laparoscopic-assisted pre-pubic urethrostomy (hybrid laparoscopic) in a cat with urethral stricture. A five-years-old cat presenting recurrent feline lower urinary tract disease (FLUTD) had undergone to perineal urethrostomy two years and nine months ago. After this procedure, the patient underwent another four corrective surgeries without success. Laboratory tests (blood count and biochemical) and image diagnosis were realized. The patient was subjected to laparoscopic-assisted pre-pubic urethrostomy and it was achieved therapeutic success and adequate patient quality of life. It is concluded that this technique is feasible and safe in cats, making it a possible choice of surgical treatment for urethral stricture.A uretrostomia é técnica cirúrgica utilizada em casos de obstrução parcial ou total da uretra, e na abordagem laparoscópica pode ser uma opção de tratamento. Devido à escassez de estudos envolvendo essa técnica, este trabalho relata o caso de uretrostomia pré-púbica videoassistida (laparoscópica híbrida) em um felino com estenose uretral. O paciente de cinco anos de idade apresentando histórico de doença do trato urinário inferior dos felinos (DTUIF) recorrente, havia sido submetido a uretrostomia perineal há dois anos e nove meses. Depois desse procedimento, o paciente passou por mais quatro cirurgias corretivas sem sucesso. Após realização de exames laboratoriais (hemograma e bioquímicos) e de imagem, o animal foi submetido a uretrostomia pré-púbica laparoscópica híbrida, obtendo-se sucesso terapêutico e possibilitando ao paciente adequada qualidade de vida. Conclui-se que esta técnica é viável e segura em gatos, tornando-a uma possível escolha de tratamento cirúrgico para estenose uretral

HTLV-1 in pregnant women from the Southern Bahia, Brazil: a neglected condition despite the high prevalence.

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2014-07-22T16:06:33Z No. of bitstreams: 1 Mello MA HTLV-1 in pregnant....pdf: 282662 bytes, checksum: b8aeee59c3495253c134ba4f12367c6f (MD5)Made available in DSpace on 2014-07-22T16:06:33Z (GMT). No. of bitstreams: 1 Mello MA HTLV-1 in pregnant....pdf: 282662 bytes, checksum: b8aeee59c3495253c134ba4f12367c6f (MD5) Previous issue date: 2014Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Púbica. Salvador, BA, Brasil / Faculdade de Ilhéus. Ilhéus, BA, BrasilUniversidade Estadual de Santa Cruz. Ilhéus, BA, BrasilUniversidade Estadual do Sudoeste da Bahia. Vitória da Conquista, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Laboratório de Hematologia, Genética e Biologia Computacional. Salvador, BA, BrasilUniversidade Estadual de Santa Cruz. Ilhéus, BA, BrasilUniversidade Estadual de Santa Cruz. Ilhéus, BA, BrasilEscola Bahiana de Medicina e Saúde Pública. Salvador, BA, BrasilUniversidade Estadual de Santa Cruz. Ilhéus, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Epidemiologia Molecular e Bioestatística. Salvador, BA, BrasilUniversidade Estadual de Santa Cruz. Ilhéus, BA, BrasilUniversidade Estadual de Santa Cruz. Ilhéus, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Púbica. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Púbica. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, BrasilUniversidade Estadual de Santa Cruz. Ilhéus, BA, BrasilBACKGROUND: As the most frequent pathway of vertical transmission of HTLV-1 is breast-feeding, and considering the higher prevalence in women, it is very important to perform screening examinations for anti-HTLV-1 antibodies as part of routine prenatal care. So far, no studies of HTLV-1 seroprevalence in pregnant women in the Southern region of Bahia, Brazil, have been described. METHODS: Pregnant women were selected at the two regional reference centers for health care from Southern Bahia. A total of 2766 pregnant women attending the antenatal unit between November 2008 and May 2010 have been analyzed. An extra blood sample was drawn during their routine antenatal testing. A standardized questionnaire was applied and all positive plasma samples were tested by ELISA and were confirmed by Western Blot and PCR. Besides that, positive women were contacted and visited. The family members that were present during the visit were asked to be serologically screened to the virus. A prospective study was also carried out and newborns were followed up to two years for evaluation of vertical transmission. RESULTS: HTLV prevalence was 1.05% (CI 95%: 0.70-1.50). There was no association of HTLV-1 infection with age, education, income and ethnic differences. The association with marital status was borderline (OR = 7.99; 95% CI 1.07-59.3; p = 0.042). In addition, 43 family members of the HTLV-1 seropositive women have been analyzed and specific reactivity was observed in 32.56%, including two children from previous pregnancy.Conclusion: It is very important to emphasize that the lack of HTLV-1 screening in pregnant women can promote HTLV transmission especially in endemic areas. HTLV screening in this vulnerable population and the promotion of bottle-feeding for children of seropositive mothers could be important cost-effective methods to limit the vertical transmission. Besides that, our data reinforce the need to establish strategies of active surveillance in household and family contacts as important epidemiological surveillance actions for the early detection of virus infection and the prevention of transmission by sexual or and parenteral contact

V diretriz da Sociedade Brasileira de Cardiologia sobre tratamento do infarto agudo do miocárdio com supradesnível do segmento ST

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