Molecular identification of trichoderma isolates from sugarcane bagasse based on Internal Transcribed Spacer (ITS) rDNA

This study aimed to identify Trichoderma from sugarcane bagasse based on iinternal transcribed spacer (ITS) rDNA. The sample used was pure isolate of Trichoderma from sugarcane bagasse. DNA samples were isolated using the modified CTAB method. DNA was amplified using the primers ITS1 and ITS4. Interestingly, the results showed that Trichoderma genomic DNA has a concentration of 92.56 mg with a purity of 1.91. The amplicon of Trichoderma DNA is about 600 bp, whereas phylogenetic analysis shows that the sample of Trichoderma from sugarcane bagasse is one group with Trichoderma harzianum, Trichoderma piluliferum, Trichoderma sp. SQR339, Hypocrea nigricans, and Trichoderma sp. NFML CH12 BB. 15, Trichoderma aureoviride, Hypocrea lixii, and Trichoderma BAB-4585

Biodiversity of Edible Macrofungi from Alas Purwo National Park

This research aimed to invent edible macrofungi in Alas Purwo National Park that can be used as alternative food sources. The method that was used in this research was the explorative method with descriptive analysis. The sampling proccess took place along the side trail of the tropical rainforest ecosystem of Alas Purwo National Park. The tropical rainforest ecosystem that was reached from Triangulasi Resort with geographics coordinates 8° 39' 20.9" S 114° 21' 39.2" E then going to northwest for around three kilometres until Sadengan Track with geographics coordinates 8° 39' 13.1" S 114° 22' 18.2" E. The other track was from Trangulasi Resort with geographics coordinates 8° 39' 20.9" S 114° 21' 39.2" E then going to east for around five kilometres until Pancur Track with geographics coordinates 8° 40' 38.91" S 114° 22' 25.90" E. The collections of macrofungi samples were limited until five metres left and five metres right of the track. The result of this research showed there were 40 different genera and 22 of them are edible. Those 22 genera are Auricularia, Cantharellus, Clavulina, Collybia, Coltricia, Coprinus, Crepidotus, Dictyophora, Flammulina, Geastrum, Hydnellum, Inocybe, Leucocoprinus, Marasmius, Meripilus, Mycena, Oligoporus, Peziza, Pleurotus, Polyporus, Psathyrella, and Tremella

Biological Activity of Kencur (Kaempferia Galanga L.) Against SARS-CoV-2 Main Protease: in Silico Study

COVID-19 is a syndrome affecting pulmonary function but rather in serious conditions leads to death. Kencur (Kaempferia galanga L.) is a type of rhizome plant in Indonesia that is used as an herbal medicine called Jamu because it is believed to be able to cure various types of diseases. One of which is for anti-virus. The goal of this study was to see how effective the compounds in kencur are against COVID-19 with a molecular docking strategy. Kencur biological activities were obtained from the library and the design of the Acute Respiratory Syndrome Main protease (Mpro) has been gained from the protein data bank website. In addition, the biological activities in kencur were examined utilizing Lipinski's five-point concept was used to evaluate their substance molecular characteristics. Molecular docking analysis was performed with the PyRx Virtual Screening Tool software. The PyRx program was used for molecular docking simulation. While, the Discovery Studio Visualizer program was used to visualize the interaction between SARS-CoV-2 (Mpro) and the pharmacologically active metabolites in kencur. The docking evaluation on three antiviral substances revealed that Quercetin had the lowest binding energy when bound with Mpro and thus had the greatest potential as a viral inhibitor