This research aimed to invent edible macrofungi in Alas Purwo National Park that can be used as alternative food sources. The method that was used in this research was the explorative method with descriptive analysis. The sampling proccess took place along the side trail of the tropical rainforest ecosystem of Alas Purwo National Park. The tropical rainforest ecosystem that was reached from Triangulasi Resort with geographics coordinates 8° 39' 20.9" S 114° 21' 39.2" E then going to northwest for around three kilometres until Sadengan Track with geographics coordinates 8° 39' 13.1" S 114° 22' 18.2" E. The other track was from Trangulasi Resort with geographics coordinates 8° 39' 20.9" S 114° 21' 39.2" E then going to east for around five kilometres until Pancur Track with geographics coordinates 8° 40' 38.91" S 114° 22' 25.90" E. The collections of macrofungi samples were limited until five metres left and five metres right of the track. The result of this research showed there were 40 different genera and 22 of them are edible. Those 22 genera are Auricularia, Cantharellus, Clavulina, Collybia, Coltricia, Coprinus, Crepidotus, Dictyophora, Flammulina, Geastrum, Hydnellum, Inocybe, Leucocoprinus, Marasmius, Meripilus, Mycena, Oligoporus, Peziza, Pleurotus, Polyporus, Psathyrella, and Tremella