POLYMORPHISM OF CANDIDATE GENES OF HEMOSTASIS DISORDERS IN PREGNANT WOMEN AFTER HAVING А(H1N1)PDM2009 INFLUENZA

We studied polymorphisms of candidate genes of hemostasis disorders in 205 pregnant women after having A(H1N1) pdm2009 influenza during pregnancy and in 75 pregnant women who didn't have acute respiratory viral infections during pregnancy. Pregnant women with influenza were more often carriers of mutant alleles F2, genotypes F7:0976GA and F13:W3G> . Severe course of influenza is associated with genotypes F7:0976GA and PAI-1:4G4G. It was revealed that polymorphisms of genes F7:0976G>A, F13:103G>T and PAI-1:6755G>4G can be molecular predictors of complicated course of A(HlNl)pdm09 influenza in pregnant women

An innovative approach to the treatment of high-risk HPV patients using allokin-alpha

Introduction. In the research literature, there have been recorded instances of imbalance of interleukins and tumour necrosis factors in the cervix tissues, endocervical mucus, suggestive of the potential feasibility of the use of immunomodulatory therapy.Purpose of the study. To assess the effectiveness of the use of the domestic drug allokin-alpha in the treatment of high-risk HPV infection (HPV) in patients of reproductive age, without elimination of the virus within more than 6–12 months and planning pregnancy.Materials and methods. The study included 60 patients of reproductive age with chronic HPV carriage – BP infection. They are represented in the study by two groups: 1st: 30 people in whose therapy allokin-alpha was not used (control group); and 30 patients without HPV BPV elimination in terms of more than 6–12 months, planning pregnancy and taking the domestic cytokiton-like immunomodulator allokin-alpha.Results. Spontaneous pregnancy occurred in 2/30 (6.7%) in the group without immunomodulator and in 9/30 patients in the group with allokin-alpha (30%) cases (c² = 5.45; p = 0.02; OR = 6.0 [1.17–30.72])Conclusion. The sustained virological response that we have achieved indicates the advisability of the use of allokin-alpha, a domestic immunomodulator of natural origin, in patients with high-risk chronic HPV infection and reproductive planning

PREVALENCE AND CHARACTERISTICS OF INFERTILITY IN YOUNG WOMEN OF REPRODUCTIVE AGE LIVING IN ZABAYKALSKY DISTRICT

We conducted epidemiological research of WHO record for the estimation of prevalence and characteristics of infertility in young women living in Zabaykalsky District. The research included 2932 women of 18-35 years, 731 of them had infertility. Frequency of infertility exceeds 15% critical level, determined by WHO, and reaches 24,9 % (22,3 % - in town, 25,8 % - in countryside). Secondary infertility appears more often that primary one (54,2 % vs 45,8 %). 44,0 % of young infertile women don't plan pregnancy

Associative connection of infectious and inflammatory diseases in pregnancy and severe preeclampsia

Materials and methods. This retrospective case-control study enrolled 50 women with severe preeclampsia and 50 control women with spontaneous singleton pregnancy. Median age of women ranged from 20 to 35 years. All women did not have a history of hypertension, autoimmune, metabolic, renal, or cardiac diseases, and preeclampsia before this pregnancy. We have analyzed χ2, odds ratio (OR) and its 95% confidence intervals (95% Cl). Results. We found significant association between maternal systemic infectious and severe preeclampsia (OR = 49.6; 95% Cl 13.05-188.64). The risk of severe preeclampsia were significantly lower in patients with local infections of the lower genital tract (OR = 4.5; 95% Cl 1.49-6.71). Asymptomatic bacteriuria is associated with the highest risk of severe preeclampsia (OR = 17.0; 95% Cl 4.66-61.81). Acute gravidarum pyelonephritis showed lower association with severe preeclampsia (OR = 5.4; 95% Cl 1.69-10.54). We did not observe increased risk of severe preeclampsia with acute respiratory infections (OR = 2.0; 95% Cl 0.71-4.69). Acute non-specific bacterial vaginitis and acute candidiasis vulvovaginitis were found to be risk factors of severe preeclampsia (OR = 6.7; 95% Cl 1.90-11.02 and OR = 4.3; 95% Cl 1.45-9.99 respectively). Cytomegalovirus infection (2 %), toxoplasmosis (2 %), Chlamydia trachomatis cervicitis (4 %), acute Trichomonas colpitis (2 %) and bacterial vaginosis (4 %) were found only in patients with severe preeclampsia. Conclusion. Our data support that acute maternal infection is associated with an increased risk of severe preeclampsia in healthy women with singleton pregnancy. Systemic inflammatory response might be the main potential mechanisms related to infections and enhanced development of severe preeclampsia. Further research is required to elucidate the underlying mechanism of this association

EFFICIENCY, SAFETY AND POSSIBILITIES OF IMMUNOTROPIC THERAPY AFTER SURGICAL TREATMENT OF ENDOMETRIOSIS

The identification of the immune system disorders along with the impact on the causative agent and correction of such disorders is of great importance in the therapy of patients with endometritis. It has been established that the infectious agents are eliminated by the phagocytic system cells, therefore the optimal choice for the activation of anti-infective immunity are the immunomodulators acting on the monocyte-macrophage system cells. Use of immunomodulators: recombinant human interleukin-2 (Roncoleukin®, IL-2) and Interferon alfa-2b + Taurine + Benzocaine (Genferon®) in combination with the standard therapy showed its safety and efficiency in treating patients with endometritis after caesarean section

COMPARATIVE EFFECTS OF THYMOGEN AND THYMALIN UPON IMMUNITY, HAEMOSTASIS AND POSTOPERATIVE ENDOMETRITIS

Abstract.  An  immune  deficiency  state,  along  with  continuously  increased  intravascular  coagulation, are  shown  to  develop  in  puerperal  women  after  Caesarian  section  complicated  by  endometritis.  Complex therapeutic schedules aimed for endometritis treatment, including thymic peptides (thymalin or thymogen) are accompanied by more rapid correction of these disorders and favorable clinical effect. (Med. Immunol., 2011,  vol. 13, N 2-3, pp 279-284

Increasing the effectiveness of the university based on economics modeling

Исследовательская статья с элементами кейса и иллюстрацией конкретных расчетов на основе разработанной модели с использованием данных пилотного вуза. Цель - сформировать модель поддержки управленческих решений по эффективному использованию имущества вуза, провести исследование на основе такой модели и предложить рекомендации по повышению эффективности деятельности вуза в целом. В статье анализируется достаточность бюджета для содержания имущества вуза и обеспечения его текущей деятельности, а также рассматриваются возможности повышения эффективности использования имущества, предлагается модель поддержки принятия управленческих решений в отношении недвижимого имущества вуза. Данная модель формируется из нескольких подмоделей: «учебная деятельность», «научная деятельность», «проживание», «стоимость использования», «развитие имущественного комплекса», «бюджет». Она может использоваться для имитационных расчетов «что если» с целью анализа различных сценариев изменения параметров внешней среды и внутренних параметров управления (в частности, сценариев по достижению стратегических целей вуза) и прогноза результатов деятельности на основе расчетных показателей вуза. Методологической основой исследования явились общенаучные методы, такие как анализ и синтез, моделирование и классификация, а также методы теории принятия решений. Имитационные расчеты позволяют выделить наиболее важные (существенные) факторы влияния на финансовый результат вуза. Например, сценарные и параметрические расчеты показывают, что для вузов с высокой степенью загруженности помещений рост количества студентов (контрольных показателей приема) при прежней эффективности использования помещений требует строительства новых зданий и в текущих условиях функционирования возможен только при поддержке государства. Повышение эффективности использования имущества позволяет обеспечить рост целевых показателей вуза без дополнительных инвестиций со стороны государства. В условиях снижения контингента студентов модель позволяет оценить необходимую величину оптимизации удельных экономических показателей для формирования бездефицитного бюджета. Новым в статье является разработка и построение модели, которая в комплексе увязывает учебную и научную деятельность вуза, формирование расписания для студентов и загрузку аудиторий, содержание помещений и общежитий, а также бюджет вуза. Подход универсален и применим в любом учреждении высшего образования. Разработанная модель может послужить методической основой для тиражирования предложенного подхода и поиска путей повышения эффективности организаций высшего образования. Применение модели для параметров (условий) конкретных вузов позволит их руководству целенаправленно задавать параметры развития вуза для рационального применения программно-целевого подхода по развитию и достижению целевых показателей. В будущем исследователи могут выполнить более детальные эмпирические разработки по совершенствованию с учетом многообразия внутренних и внешних факторов. Результаты данного исследования имеют практическую значимость для проректоров по экономике, административно-хозяйственной работе, начальников финансово-экономических управлений, а также для руководителей вузов, принимающих решения о формировании стратегии развития в условиях ограниченного бюджета с учетом рисков.The article is the research paper, with case elements and illustration of specific calculations based on the developed model using the data of a pilot university. Its goal is to form a model for decision support system for the effective use of the institution’s property, investigate the model for specific university’s data and propose recommendations for improving the effectiveness of the university as a whole. The problem of analyzing the budget sufficiency for maintaining the university’s assets and ensuring its current activities is examined in the paper, as well as the opportunity of increasing the efficiency of the use of property. The model is proposed for decision support system for the property of the institution. This model is formed from several submodels: «educational activity», «scientific activity», «living activity», «expenses», «development of the property», and «budget». The model can be used for simulation «what if» analysis of different scenarios of the external and internal parameters (in particular, to estimate the achievement of the strategic goals of the university) and forecasting the results of activities. The methodological basis of the research included general scientific methods, such as analysis and synthesis, modeling and classification, as well as methods of decision theory. Simulation calculations allow us to identify the most significant factors in the impact on the financial result of the university. In particular, for the scenario of high degree of occupancy of premises, the growth in the number of students (admission control indicators) requires the construction of new buildings under current operating conditions and is possible only with the support of the state. The scenario of increasing the efficiency of the use of property makes it possible to achieve the growth of the target indicators of the university without additional investments from the state. For the scenario of a decrease in the contingent of students, the model allows the estimation of the necessary amount of optimization of the specific economic parameters for the formation of a no-deficit budget. The complex covers the educational and scientific activities of the university, the formation of a timetable for students and the occupancy of audiences, maintenance of rooms and hostels, as well as the budget of the university making the developed model new. It is expected that this approach is applicable for different institutions of higher education. The application of the model for the conditions of specific universities will allow their management to set the parameters for the development of the university for the goal-targeted approach for the development. In the future, researchers can develop the model under detailed empirical data, taking into account the variety of internal and external factors. The results of this study van be of practical importance for vice-rectors on economics, administrative affairs, heads of financial and economic departments as well as university executives responsible for taking decisions on strategy development in the context of limited budget taking into account risks

Study of the Polysaccharide Production by the Microalga Vischeria punctata in Relation to Cultivation Conditions

Vischeria punctata is a unicellular microalga that has industrial potential, as it can produce substances with beneficial properties. Among them, endopolysaccharides (accumulated in cells) and exopolysaccharides (released by cells into the culture medium) are of particular interest. This study aimed to investigate the effect of nutrient medium composition on the growth of V. punctata biomass and the synthesis of polysaccharides by microalgae. The effect of modifying a standard nutrient medium and varying cultivation parameters (temperature, time, and extractant type) on the yield of exopolysaccharides produced by the microalgae V. punctate was investigated. The methods of spectrophotometry, ultrasonic extraction, and alcohol precipitation were used in the study. It was found that after 61 days of cultivation, the concentration of polysaccharides in the culture medium was statistically significantly higher (p <0.05) when using a Prat nutrient medium (984.9 mg/g d.w.) than BBM 3N (63.0 mg/g d.w.). It was found that the increase in the V. punctata biomass when cultivated on different nutrient media did not differ significantly. The maximum biomass values on Prat and BBM 3N media were 1.101 mg/g d.w. and 1.120 mg/g d.w., respectively. Neutral sugars and uronic acids were found in the culture media. It follows on from the obtained data that the modified PratM medium was more efficient for extracting polysaccharides from V. punctata. The potential of microalgae as new sources of valuable chemicals (polysaccharides), which can be widely used in technologies for developing novel functional foods, biologically active food supplements, and pharmaceutical substances, was studied

Study of the polysaccharide production by the microalgae C-1509 Nannochloris sp. Naumann

Biologically active compounds, including polysaccharides isolated from microalgae, have various properties. Although Nannochloropsis spp. have the potential to produce secondary metabolites important for biotechnology, only a small part of the research on these microalgae has focused on their ability to produce polysaccharide fractions. This study aims to evaluate the physicochemical growth factors of Nannochloropsis spp. microalgae, which ensure the maximum accumulation of polysaccharides, as well as to optimize the parameters of polysaccharide extraction. The optimal nutrient medium composition was selected to maximize biomass and polysaccharide accumulation. The significance of selecting the extraction module and extraction temperature regime, as well as the cultivation conditions (temperature and active acidity value) is emphasized. Important chemical components of polysaccharides responsible for their biological activity were identifie