2,522 research outputs found
Marine ecosystem services: Linking indicators to their classification
© 2014 Elsevier Ltd. All rights reserved. There is a multitude of ecosystem service classifications available within the literature, each with its own advantages and drawbacks. Elements of them have been used to tailor a generic ecosystem service classification for the marine environment and then for a case study site within the North Sea: the Dogger Bank. Indicators for each of the ecosystem services, deemed relevant to the case study site, were identified. Each indicator was then assessed against a set of agreed criteria to ensure its relevance and applicability to environmental management. This paper identifies the need to distinguish between indicators of ecosystem services that are entirely ecological in nature (and largely reveal the potential of an ecosystem to provide ecosystem services), indicators for the ecological processes contributing to the delivery of these services, and indicators of benefits that reveal the realized human use or enjoyment of an ecosystem service. It highlights some of the difficulties faced in selecting meaningful indicators, such as problems of specificity, spatial disconnect and the considerable uncertainty about marine species, habitats and the processes, functions and services they contribute to
Impact of Temporal Macropore Dynamics on Infiltration : Field Experiments and Model Simulations
Macropores greatly affect water and solute transport in soils. Most macropores are of biogenic origin; however, the resulting seasonal dynamics are often neglected. Our study aimed to examine temporal changes in biopore networks and the resulting infiltration patterns. We performed infiltration experiments with Brilliant Blue on pastureland in the Luxembourgian Attert catchment (spring, summer, and autumn 2015). We developed an image-processing scheme to identify and quantify changes in biopores and infiltration patterns. Subsequently, we used image-derived biopore metrics to parameterize the ecohydrological model echoRD (ecohydrological particle model based on representative domains), which includes explicit macropore flow and interaction with the soil matrix. We used the model simulations to check whether biopore dynamics affect infiltration. The observed infiltration patterns revealed variations in both biopore numbers and biopore–matrix interaction. The field-observed biopore numbers varied over time, mainly in the topsoil, with the largest biopore numbers in spring and the smallest in summer. The number of hydrologically effective biopores in the topsoil seems to determine the number and thereby the fraction of effective biopores in the subsoil. In summer, a strong biopore–matrix interaction was observed. In spring, the dominant process was rapid drainage, whereas in summer and autumn, most of the irrigated water was stored in the examined profiles. The model successfully simulated infiltration patterns for spring, summer, and autumn using temporally different macropore setups. Using a static macropore parameterization the model output deviated from the observed infiltration patterns, which emphasizes the need to consider macropores and their temporal dynamics in soil hydrological modeling
Capturing the short-term variability of carbon dioxide emissions from sedimentary rock weathering in a remote mountainous catchment, New Zealand
Weathering of organic carbon contained in sedimentary rocks (petrogenic OC, OCpetro) is an important control on the concentrations of carbon dioxide (CO2) and oxygen in the atmosphere. Of particular significance are steep mountainous catchments, where high rates of physical erosion introduce OCpetro to the surface, where oxygen in air and water can help drive oxidative weathering reactions, yet measurements of CO2 emissions from OCpetro oxidation are still scarce. Here, we use in situ gas accumulation chambers and show that CO2 fluxes, and their environmental controls, can be determined during a stand-alone, short-term (8 days) field campaign, applied to a remote setting. In the rapidly eroding Waiapu River catchment, New Zealand, dominated by mudstones, we measured high rates of CO2 release (222–1590 mgC m−2 d−1) in five accumulation chambers in the near-surface of naturally fractured and bedded rock outcrops. The corresponding CO2 concentrations are very high (pCO2 ~4700–27,100 ppmv), and such values could influence acid-hydrolysis reactions during chemical weathering of co-occurring silicate minerals. The CO2 is radiocarbon depleted (fraction modern, F14C = 0.0122–0.0547), confirming it is petrogenic in origin. Stable carbon isotopes suggest a source from OCpetro, but δ13C values of the CO2 are lower by ~3.5–3.7 ± 0.1 ‰ from those of OCpetro (−25.9 ± 0.1 ‰), consistent with isotope fractionation associated with microbial respiration of OCpetro. Over 6 days of measurement, we find that CO2 fluxes respond quickly to changes in temperature and humidity, indicating an environmental regulation that is captured by our short-term installation. The approaches applied here mean that future research can now seek to constrain the climatic, lithological and biological controls on OCpetro oxidation across regional to global scales
Increased use of total shoulder arthroplasty for osteoarthritis and improved patient-reported outcome in Denmark, 2006-2015:a nationwide cohort study from the Danish Shoulder Arthroplasty Registry
Parathyroid hormone stimulates bone regeneration in an atrophic non-union model in aged mice
Background Non-union formation still represents a major burden in trauma and orthopedic surgery. Moreover, aged
patients are at an increased risk for bone healing failure. Parathyroid hormone (PTH) has been shown to accelerate
fracture healing in young adult animals. However, there is no information whether PTH also stimulates bone regeneration in atrophic non-unions in the aged. Therefore, the aim of the present study was to analyze the efect of PTH
on bone regeneration in an atrophic non-union model in aged CD-1 mice.
Methods After creation of a 1.8 mm segmental defect, mice femora were stabilized by pin-clip fxation. The animals
were treated daily with either 200 mg/kg body weight PTH 1–34 (n=17) or saline (control; n=17) subcutaneously.
Bone regeneration was analyzed by means of X-ray, biomechanics, micro-computed tomography (µCT) imaging
as well as histological, immunohistochemical and Western blot analyses.
Results In PTH-treated animals bone formation was markedly improved when compared to controls. This was associated with an increased bending stifness as well as a higher number of tartrate-resistant acid phosphatase (TRAP)-
positive osteoclasts and CD31-positive microvessels within the callus tissue. Furthermore, PTH-treated aged animals showed a decreased infammatory response, characterized by a lower number of MPO-positive granulocytes
and CD68-positive macrophages within the bone defects when compared to controls. Additional Western blot
analyses demonstrated a signifcantly higher expression of cyclooxygenase (COX)-2 and phosphoinositide 3-kinase
(PI3K) in PTH-treated mice.
Conclusion Taken together, these fndings indicate that PTH is an efective pharmacological compound for the treatment of non-union formation in aged animals
Inside Out: Modern Imaging Techniques to Reveal Animal Anatomy
Animal anatomy has traditionally relied on detailed dissections to produce anatomical illustrations, but modern imaging modalities, such as MRI and CT, now represent an enormous resource that allows for fast non-invasive visualizations of animal anatomy in living animals. These modalities also allow for creation of three-dimensional representations that can be of considerable value in the dissemination of anatomical studies. In this methodological review, we present our experiences using MRI, CT and μCT to create advanced representation of animal anatomy, including bones, inner organs and blood vessels in a variety of animals, including fish, amphibians, reptiles, mammals, and spiders. The images have a similar quality to most traditional anatomical drawings and are presented together with interactive movies of the anatomical structures, where the object can be viewed from different angles. Given that clinical scanners found in the majority of larger hospitals are fully suitable for these purposes, we encourage biologists to take advantage of these imaging techniques in creation of three-dimensional graphical representations of internal structures
Direct measurement of the Meissner screening profile in superconductor-superconductor bilayers using low-energy muon spin rotation
Superconducting radio frequency (SRF) cavities, which are critical components
in many particle accelerators, need to be operated in the Meissner state to
avoid strong dissipation from magnetic vortices. For a defect-free
superconductor, the maximum attainable magnetic field for operation is set by
the superheating field, , which directly depends on the
surface current. In heterostructures composed of different superconductors, the
current in each layer depends not only on the properties of the individual
material, but also on the electromagnetic response of the adjacent layers
through boundary conditions at the interfaces. Three prototypical bilayers
[(50 nm)/Nb, (80 nm)/Nb, and
(160 nm)/Nb] are investigated here by depth-resolved
measurements of their Meissner screening profiles using low-energy muon spin
rotation (LE-SR). From fits to a model based on London theory (with
appropriate boundary and continuity conditions), a magnetic penetration depth
for the thin layers of 182.5(31) nm is found, in good agreement with literature values for the bulk
alloy. In contrast, a simple London model without appropriate boundary
conditions overestimates by more than a factor
of two, suggesting that it is inappropriate for quantifying
here. Using the measured
, the maximum vortex-free field,
, of the superconductor-superconductor (SS) bilayer structure
was estimated to be 610(40) mT. The strong suppression of the surface current
in the layer suggests an optimal thickness of 261(14) nm.Comment: 13 pages and 8 figure
Radiographic, Biomechanical and Histological Characterization of Femoral Fracture Healing in Aged CD-1 Mice
With a gradually increasing elderly population, the treatment of geriatric patients represents
a major challenge for trauma and reconstructive surgery. Although, it is well established that aging
affects bone metabolism, it is still controversial if aging impairs bone healing. Accordingly, we
investigated fracture healing in young adult (3–4 months) and aged (16–18 months) CD-1 mice using
a stable closed femoral fracture model. Bone healing was analyzed by radiographic, biomechanical
and histological analysis at 1, 2, 3, 4 and 5 weeks after fracture. Our results demonstrated an increased
callus diameter to femoral diameter ratio in aged animals at later time points of fracture healing
when compared to young adult mice. Moreover, our biomechanical analysis revealed a significantly
decreased bending stiffness at 3 and 4 weeks after fracture in aged animals. In contrast, at 5 weeks
after fracture, the analysis showed no significant difference in bending stiffness between the two study
groups. Additional histological analysis showed a delayed endochondral ossification in aged animals
as well as a higher amounts of fibrous tissue at early healing time points. These findings indicate a
delayed process of callus remodeling in aged CD-1 mice, resulting in a delayed fracture healing when
compared to young adult animals. However, the overall healing capacity of the fractured femora was
not affected by aging
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Genetic reporter analysis reveals an expandable reservoir of OCT4+ cells in adult skin
The transcription factor Oct4 (Pou5f1) is a critical regulator of pluripotency in embryonic and induced pluripotent stem cells. Therefore, Oct4 expression might identify somatic stem cell populations with inherent multipotent potential or a propensity for facilitated reprogramming. However, analysis of Oct4 expression is confounded by Oct4 pseudogenes or non-pluripotency-related isoforms. Systematic analysis of a transgenic Oct4-EGFP reporter mouse identified testis and skin as two principle sources of Oct4+ cells in postnatal mice. While the prevalence of GFP+ cells in testis rapidly declined with age, the skin-resident GFP+ population expanded in a cyclical fashion. These cells were identified as epidermal stem cells dwelling in the stem cell niche of the hair follicle, which endogenously expressed all principle reprogramming factors at low levels. Interestingly, skin wounding or non-traumatic hair removal robustly expanded the GFP+ epidermal cell pool not only locally, but also in uninjured skin areas, demonstrating the existence of a systemic response. Thus, the epithelial stem cell niche of the hair follicle harbors an expandable pool of Oct4+ stem cells, which might be useful for therapeutic cell transfer or facilitated reprogramming. Electronic supplementary material The online version of this article (doi: 10.1186/2045-9769-3-9) contains supplementary material, which is available to authorized users
Anti-MOG antibodies are present in a subgroup of patients with a neuromyelitis optica phenotype
Background: Antibodies against myelin oligodendrocyte glycoprotein (MOG) have been identified in a subgroup of pediatric patients with inflammatory demyelinating disease of the central nervous system (CNS) and in some patients with neuromyelitis optica spectrum disorder (NMOSD). The aim of this study was to examine the frequency, clinical features, and long-term disease course of patients with anti-MOG antibodies in a European cohort of NMO/NMOSD. Findings: Sera from 48 patients with NMO/NMOSD and 48 patients with relapsing-remitting multiple sclerosis (RR-MS) were tested for anti-aquaporin-4 (AQP4) and anti-MOG antibodies with a cell-based assay. Anti-MOG antibodies were found in 4/17 patients with AQP4-seronegative NMO/NMOSD, but in none of the AQP4-seropositive NMO/NMOSD (n = 31) or RR-MS patients (n = 48). MOG-seropositive patients tended towards younger disease onset with a higher percentage of patients with pediatric (<18 years) disease onset (MOG+, AQP4+, MOG-/AQP4-: 2/4, 3/31, 0/13). MOG-seropositive patients presented more often with positive oligoclonal bands (OCBs) (3/3, 5/29, 1/13) and brain magnetic resonance imaging (MRI) lesions during disease course (2/4, 5/31, 1/13). Notably, the mean time to the second attack affecting a different CNS region was longer in the anti-MOG antibody-positive group (11.3, 3.2, 3.4 years). Conclusions: MOG-seropositive patients show a diverse clinical phenotype with clinical features resembling both NMO (attacks mainly confined to the spinal cord and optic nerves) and MS with an opticospinal presentation (positive OCBs, brain lesions). Anti-MOG antibodies can serve as a diagnostic and maybe prognostic tool in patients with an AQP4-seronegative NMO phenotype and should be tested in those patients
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