Empirical Insights in the Current Development of Smart Contracts

Blockchain technology enables a lot of knowledge possibilities in an even more digital business environment. Besides the well-known bitcoin application, another implementation so called smart contracts recently arised. Smart contracts profit from the blockchain mechanism benefits (e.g., transaction security). A huge advantage of smart contracts is, that they provide trust between transaction partners without integrating a third party. Practice has already noticed their advantages and implementing smart contracts more and more into their business. However, research is still under development and a common scientifical foundation is still missing. Particularly, research lacks in empirical and practical findings. This paper responds to that gap. Conducting an expert study relevant data was collected and analyzed regarding empirical standards. The results we found and present in this on-going research paper give insights about basic aspects, challenges in the implementation as well as the use cases of smart contracts

Holocene coastal stratigraphy, coastal changes and potential palaeoseismological implications inferred from geo-archives in Central Chile (29–32° S)

Coastal geomorphology and the stratigraphy of coastal geoarchives record past coastal and fluctuations of coastal environments. In addition, these archives potentially store traces of past extreme events such as earthquakes and tsunamis, severe storms, and major flfl oodings of the coastal hinterland, e.g. due to El Niño conditions. Studying their characteristics may thus improve the knowledge of past frequency and magnitude patterns of such extreme events. For instance, large scaled spatial information about past earthquakes is needed for the understanding and estimation of seismo-tectonic processes. Misinterpretations in the size of preceding earthquakes may lead to incorrect strain balance estimations along megathrusts. Thus, fundamental research on the occurrence of past earthquakes is needed, which can be reflected in sudden or long-term coastal changes. Using sedimentological, geomorphological and microfaunal evidence, coeval geomorphodynamic and palaeoenvironmental changes at four different locations between 29° 50′ and 32° 20′ S in Central Chile were identififi ed in estuary systems, coastal swamps and coastal plains. The results may represent possible indirect evidence for palaeoseismicity, affecting the coastal system by vertical tectonic movements. Changes of coastline elevation, morphodynamic activity and/or coastal environments, as well as the formation of a liquefaction layer took place during the last c. 400 years. Moreover, major flfl ooding events related to strong El Niño conditions are assumed to have influenced the coastal stratigraphy by depositing high energy fluvial deposits. Our results suggest that the coastal environment, geomorphology and stratigraphy are considerably inflfl uenced by tectonic processes in the study area; a relation of the presented fifi ndings to the 1730 Great Valparaíso Earthquake is assumed. In general, the findings may encourage the implementation of comparable detailed studies, which may ultimately contribute to a better understanding of the Holocene coastal evolution and its relation to palaeoseismicity in Central Chile

The environs of Elaia (NW Turkey) – Sea-level fluctuations, vegetation history and microfaunal record

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The ancient harbour city of Ainos and its environs – Palaeoenvironmental reconstructions based on sedimentological and microfaunal evidence

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The SLIM1 transcription factor affects sugar signaling during sulfur deficiency in Arabidopsis

The homeostasis of major macronutrient metabolism needs to be tightly regulated, especially when the availability of one or more nutrients fluctuates in the environment. Both sulfur metabolism and glucose signaling are important processes throughout plant growth and development, as well as during stress responses. Still, very little is known about how these processes affect each other, although they are positively connected. Here, we showed in Arabidopsis that the crucial transcription factor of sulfur metabolism, SLIM1, is involved in glucose signaling during shortage of sulfur. The germination rate of the slim1_KO mutant was severely affected by high glucose and osmotic stress. The expression of SLIM1-dependent genes in sulfur deficiency appeared to be additionally induced by a high concentration of either mannitol or glucose, but also by sucrose, which is not only the source of glucose but another signaling molecule. Additionally, SLIM1 affects PAP1 expression during sulfur deficiency by directly binding to its promoter. The lack of PAP1 induction in such conditions leads to much lower anthocyanin production. Taken together, our results indicate that SLIM1 is involved in the glucose response by modulating sulfur metabolism and directly controlling PAP1 expression in Arabidopsis during sulfur deficiency stress

Highly colored boron-doped thiazolothiazoles from the reductive dimerization of boron isothiocyanates

Reduction of (CAAC)BBr2(NCS) (CAAC=cyclic alkyl(amino)carbene) in the presence of a Lewis base L yields tricoordinate (CAAC)LB(NCS) borylenes which undergo reversible E/Z‐isomerization. The same reduction in the absence of L yields deep blue, bis(CAAC)‐stabilized, boron‐doped, aromatic thiazolothiazoles resulting from the dimerization of dicoordinate (CAAC)B(NCS) borylene intermediates

Intensiv farbige Bor-dotierte Thiazolthiazole durch reduktive Dimerisierung von Borisothiocyanaten

Die Reduktion von (CAAC)BBr2(NCS) (CAAC=cyclisches Alkyl(amino)carben) in der Gegenwart einer Lewis-Base L liefert dreifach koordinierte (CAAC)LB(NCS)-Borylene, die eine reversible (E)/(Z)-Isomerisierung eingehen. Die gleiche Reduktion in Abwesenheit von L führt zu intensiv blauen, bis(CAAC)-stabilisierten, Bor-dotierten, aromatischen Thiazolthiazolen, die aus der Dimerisierung zweifach koordinierter (CAAC)B(NCS)-Borylenintermediate resultieren

Estimating the optimal efflux inhibitor concentration of carvacrol as a function of the bacterial physiological state

Our aim was to find the optimal efflux inhibitor concentration of a natural component, carvacrol, as a function of the physiological state of Escherichia coli. Using fluorescence-based measurements with two strains of E. coli, the effect of carvacrol was assessed at 17 sub-inhibitory concentrations, at which the bacterial efflux mechanism was compromised. The efficacy of carvacrol, as an efflux inhibitor, was compared to synthetic inhibitors and we found carvacrol the most efficient one. We considered the accumulation of Ethidium Bromide (EtBr) as a proxy for drugs spreading in the cell, thus measuring the efflux activity indirectly. The change in membrane integrity caused by the exposure to carvacrol was monitored using the LIVE/DEAD BacLight Bacterial Viability kit. To find the optimal inhibitory concentration of carvacrol, we used predictive microbiology methods. This optimum varied with the bacterial physiological state, as non-growing cultures were less susceptible to the effect of carvacrol than growing cultures were. Moreover, we point out, for the first time, that the efflux-mediated resistance of untreated cultures was also stronger in the non-growing than in the growing phase at population level