Pengaruh Limbah Cair Pabrik Gula Di Kediri Terhadap Pertumbuhantanaman Eceng Gondok (Eichornia Crassipes).

Kediri is an industrial town in which the industry has become main commodity livelihood for its citizen. The rapid of technology encourages the growth of industries that have great contribution for environmental pollution; one of them is sugar factory in Kediri. This factory produces liquid waste that it contains organic and un-organic compounds which its presence trigger Eichornia Crassipes grows rapidly in the river. In addition, Eichomia Crassipes has a potential as an Agnesia cleaners. This study is aimed at determining the effect of sugar factory liquid waste to the growth of Eichornia Crassipes. True experimental type RAK was selected as a research design, in which the treatment was done by 0% (as control), 25%, 50%, 75%, and 100% and it was repeated 5 times. Observed parameter included root length, stem length, length and width of leaf. The systematic data was analyzed using STAT program version 2.6. the results showed significant differences in the growth of the Eichornia Crassipes morphology on root length, stem length, length and width of leaf width and the 100 % liquid waste of sugar factory highest average was found on the length root of stalk. On the other side, liquid waste of sugar's factory can be used as an organic fertilizer to some extent (deposited waste)

Comparison of murine B-cell proliferative response to bacterial lipopolysaccharide and DNP derivative of Mycobacterium tuberculosis antigens

The DNP derivative of sonicate antigens of the H37Ra strain ofMycobacterium tuberculosis (Ra-DNP) is known to induce marked B-cell proliferation. In order to understand whether B-cell proliferation in response to Ra-DNP was antigen driven or represented a non-specific mitogenic effect of Ra-DNP, the effect of Ra-DNP was compared with that of lipopolysaccharide a potent B-cell mitogen. Parameters used for comparison were (i) thymidine incorporation, (ii) viable cell counts, (iii) amount of lg secreted, (iv) isotype profile of Ig released and (v) cell cycling pattern of B-cells in culture. Overall the effect of Ra-DNP was found to be essentially similar to that of lipopolysaccharide for all parameters examined. Yet quantitatively, the effect of the former was always relatively poorer. At optimal doses, the effect of Ra-DNP ranged from 50 to 70% of the lipopolysaccharide effect in different assays. These results suggest that Ra-DNP may have a B-cell mitogenic effect similar to the effect of lipopolysaccharide, but all B-cells may not respond to Ra-DNP

Raising Self-Awareness and Developing Intercultural Competence by Activating Personal Filters

All individuals are fitted with filters which affect their experience of the world, and which depend on various factors, including personal traits and cultural influences. Language is a reflection of the self, and of the culture and people it belongs to, thus when learning a new language each individual is approaching a different identity and culture with personal filters which act on the learning process. Learning involves approaching something new and inevitably leads to some change, which may occur without conscious awareness but is affected by the individual’s attitude towards novelty and diversity.This joint project involved learners of English (Parma University) and of Italian (University of Salzburg) as L2, and aimed at indirectly testing these filters. The objectives were to verify if their filters are subtly expressed in their way of seeing themselves, their own language and culture, and the ‘other’ language and culture, and also raise their awareness of them. This hopefully is a stepping stone towards discovering aspects of their personality, developing sensitivity to differences and recognizing factors to be exploited to become more efficient learners

IN VITRO AND IN VIVO PROTECTIVE EFFECTS OF AMBREX, A POLYHERBAL FORMULATION, AGAINST METHOTREXATE INDUCED DAMAGES IN HEPATIC CELLS

Objective: To evaluate the hepatoprotective effect of Ambrex, a poly herbal formulation against methotrexate (MTX) induced hepatotoxicity in Swiss albino mice as well as in Chang liver cell lines.Methods: Ambrex was exposed to MTX intoxicated chang liver cells and cells were harvested for studying the gene expressions of Dihydrofolate reductase (DHFR), B-cell lymphoma 2 (BCL2) and Bcl-2-associated X protein (BAX). In in vivo study, Ambrex was administered orally for a period of 7 days at two dose levels (250 and 500 mg/kg b. wt) and MTX (20 mg/kg b. wt, i. p) was injected one hour after the last test drug administration. Protective effect of Ambrex was evaluated by measuring aspartate transaminase (SGOT), alanine transaminase (SGPT), alkaline phosphatase (ALP), γ–glutamyl transferase (γGT) and total bilirubin. Its effect on superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH) and lipid peroxide (LPO) was also determined.Results: Data revealed that Ambrex was able to restore the levels of antioxidants such as SOD, Catalase, and Glutathione to near normal and reduced the elevated plasma levels of SGOT, SGPT, ALP, γ–GT and total bilirubin. It also inhibited the formation of hepatic malondialdehyde induced by MTX. In vitro studies revealed that Ambrex protected MTX induced hepatotoxicity at the dose of 50 and 500ng/ml. Further, mRNA expression also illustrated that Ambrex inhibited the over expression of BAX and suppressed BCL2 and DHRF expressions.Conclusion: Results suggest that Ambrex has potent hepatoprotective effect which was evident from both in vivo and in vitro results.Â

Editorial : Predictive mechanisms in action, perception, cognition, and clinical disorders

Acknowledgments The authors would like to acknowledge their funding sources (NIH F32 MH117933 to AD, Alon Fellowship to LR).Peer reviewedPublisher PD

The Application of Problem Bassed Learning to Improve Learning Motivation Skills, of Scientific Inquiry and Argumentation Skills of Students SMPN Kediri on the Material Changes and Environmental Pollutan

This study aimed to measure the effectiveness of problem based learning design study on the motivation skills, of inquiry and scientific argumentation skills. This is the type of study design non-equivalent control group design in class VII SMPN 1 Grogol Kediri on 12 up to 23 May 2015 on the material changes and environmental pollutan. Used some of the instruments to support research that motivation questionnaire and inquiry skill rubric (process and product). And rubric scientific argumentation skills are integrated in the cognitive achievement tes. Data tabulated later in the analysis using the program SPSS 16 for windows 2007. The study is showed that: 1) students motivation treatment group (80%) higher than the control group (70%); 2) inquiry skills of the control group with the treatment process is the same (α= 0,181>0,05) but the product of inquiry skills between the two group differed (α= 0,01<0,05); 3) ANACOVA test study showed that the skills of scientific argument between the treatment group and the control group is different (α=0,000<0,05). Concluded that the model of problem-based learning can increase learning motivation, skills of inquiry, the ability of the scientific arguments.

Pengaruh Metode Multisensori Terhadap Kemampuan Mengingat Huruf Alfabet Pada Siswa Kelompok Bermain Di Sekolah Cahaya Nurani Jember

Mengingat huruf merupakan kemampuan pemula dalam tahapan untukmenjadi pembaca pemula, sebelum membaca pemula anak mengingat huruf,karena dengan mengingat huruf anak dapat menyerap setiap informasi yang ada dilingkungan. Selain itu ada fenomena lain yang berkaitab dengan kemampuanmengingat yaitu cara mengingat anak yang berkaitan di Sekolah Cahaya NuraniJember. Fenomena ini yang membuat peneliti tertarik untuk melakukkanpenelitian dengan “Pengaruh Metode Multisensori Terhadap KemampuanMeningat Huruf Alfabet Pada Sisiwa Kelompok Bermain Di Sekolah CahayaNurani”.Jenis penelitian yang digunakan adalah Eksperimen dengan desain pretest andposstes grup desain. Metode pengumpulan data mengunakan wawancara danobservasi serta alat yang digunakan sebagai metode multisensori. Populasipenelitian ini adalah siswa kelompok bermain besar (palaygrup). Tehnikpengambilan data menngunakan purposive sampling yaitu data yang sudah adadilapangan. Tehnik analisa menggunakan One Sampel T-Test.Berdasarkan hasil analisa yang diperoleh ada peningkat kemampuanmengingat huruf pada anak dengan nilai signifikan yang menyatakan bahwasignifikansi > p=0,05 sehingga Ho diterima maka dapat dikatakan korelasi yaituada pengarh metode multisensori terhadap kemampuan mengingat huruf padaanak

Immune response to Mycobacterium tuberculosis culture filtrate antigen in cured spinal tuberculosis patients and their spouses

Humoral and cell mediated immune responses were studied in cured spinal tuberculosis patients and their spouses to understand immunit to tuberculosis in cured patients. Antibody litre and immune complex levels were measured and lymphocyte response to Mycobaclerinm tuberculosis culture filtrate antigen was observed in cured spinal tuberculosis patients ( n = 3 0 ) and their spouses (n =27). A trend towards increased antibody litre was seen in cured patients as compared to their spouses. Significantly increased circulating immune complex levels, as measured by PEG OD280 (polyethylene glycol optical density 280) were seen in the contacts compared to cured patients. And a trend towards increased lymphocyte response to Mtuberculosis culture filtrate antigen was seen with different antigen concentrations (0.1,1 and 10 μg /ml). Moreover, the effect of active-pulmonary-Tuberculosis (AT B) plasma taken from 1ILA-DR2 positive and DR2 negative patients on lymphocyte response of the cured patients showed no dramatic immunomodulatory effect in the lymphocyte response when treated with DR2 positive or DR2 negative plasma. The study suggests that the memory response lo Mtuberculosis is well maintained even after 10-15 years of treatment

Investigating Canadian parents' HPV vaccine knowledge, attitudes and behaviour: a study protocol for a longitudinal national online survey

Introduction Human papillomavirus (HPV), a sexually transmitted infection, can cause anogenital warts and a number of cancers. To prevent morbidity and mortality, three vaccines have been licensed and are recommended by Canada’s National Advisory Committee on Immunisation (for girls since 2007 and boys since 2012). Nevertheless, HPV vaccine coverage in Canada remains suboptimal in many regions. This study will be the first to concurrently examine the correlates of HPV vaccine decision-making in parents of school-aged girls and boys and evaluate changes in parental knowledge, attitudes and behaviours over time. Methods and analysis Using a national, online survey utilising theoretically driven constructs and validated measures, this study will identify HPV vaccine coverage rates and correlates of vaccine decision-making in Canada at two time points (August–September 2016 and June–July 2017). 4606 participants will be recruited to participate in an online survey through a market research and polling firm using email invitations. Data cleaning methods will identify inattentive or unmotivated participants. Ethics and dissemination The study received research ethics board approval from the Research Review Office, Integrated Health and Social Services University Network for West-Central Montreal (CODIM-FLP-16–219). The study will adopt a multimodal approach to disseminate the study’s findings to researchers, clinicians, cancer and immunisation organisations and the public in Canada and internationally

Increased Frequency and Compromised Function of T Regulatory Cells in Systemic Sclerosis (SSc) Is Related to a Diminished CD69 and TGFβ Expression

Contains fulltext : 80239.pdf (publisher's version ) (Open Access)BACKGROUND: Regulatory T cells (Tregs) are essential in the control of tolerance. Evidence implicates Tregs in human autoimmune conditions. Here we investigated their role in systemic sclerosis (SSc). METHODS/PRINCIPAL FINDINGS: Patients were subdivided as having limited cutaneous SSc (lcSSc, n = 20) or diffuse cutaneous SSc (dcSSc, n = 48). Further subdivision was made between early dcSSc (n = 24) and late dcSSc (n = 24) based upon the duration of disease. 26 controls were studied for comparison. CD3+ cells were isolated using FACS and subsequently studied for the expression of CD4, CD8, CD25, FoxP3, CD127, CD62L, GITR, CD69 using flow cytometry. T cell suppression assays were performed using sorted CD4CD25(high)CD127(-) and CD4CD25(low)CD127(high) and CD3(+) cells. Suppressive function was correlated with CD69 surface expression and TGFbeta secretion/expression. The frequency of CD4(+)CD25(+) and CD25(high)FoxP3(high)CD127(neg) T cells was highly increased in all SSc subgroups. Although the expression of CD25 and GITR was comparable between groups, expression of CD62L and CD69 was dramatically lower in SSc patients, which correlated with a diminished suppressive function. Co-incubation of Tregs from healthy donors with plasma from SSc patients fully abrogated suppressive activity. Activation of Tregs from healthy donors or SSc patients with PHA significantly up regulated CD69 expression that could be inhibited by SSc plasma. CONCLUSIONS/SIGNIFICANCE: These results indicate that soluble factors in SSc plasma inhibit Treg function specifically that is associated with altered Treg CD69 and TGFbeta expression. These data suggest that a defective Treg function may underlie the immune dysfunction in systemic sclerosis