Spectral diffusion and 14N quadrupole splittings in absorption detected magnetic resonance hole burning spectra of photosynthetic reaction centers

Zero field absorption detected magnetic resonance hole burning measurements were performed on photosynthetic reaction centers of the bacteria Rhodobacter sphaeroides R26 and Rhodopseudomonas viridis. Extrapolation to zero microwave power yielded pseudohomogeneous linewidths of 2.0 MHz for Rhodopseudomonas viridis, 1.0 and 0.9 MHz for the protonated forms of Rhodobacter sphaeroides R26 with and without monomer bacteriochlorophyll exchanged, and 0.25 MHz as an upper limit for fully deuterated reaction centers of Rhodobacter sphaeroides R26. The measured linewidths were interpreted as being due to unresolved hyperfine interaction between the nuclear spins and the triplet electron spin, the line shape being determined by spectral diffusion among the nuclei. The difference in linewidths between Rhodobacter sphaeroides R26 and Rhodopseudomonas viridis is then explained by triplet delocalization on the special pair in the former, and localization on one dimer half on the latter. In the fully deuterated sample, four quadrupole satellites were observed in the hole spectra arising from the eight 14N nitrogens in the special pair. The quadrupole parameters seem to be very similar for all nitrogens and were determined to =1.25±0.1 MHz and =0.9±0.1 MHz. The Journal of Chemical Physics is copyrighted by The American Institute of Physics

CHEMICALLY MODIFIED PHOTOSYNTHETIC BACTERIAL REACTION CENTERS: CIRCULAR DICHROISM, RAMAN RESONANCE, LOW TEMPERATURE ABSORPTION, FLUORESCENCE AND ODMR SPECTRA AND POLYPEPTIDE COMPOSITION OF BOROHYDRIDE TREATED REACTION CENTERS FROM Rhodobacter sphaeroides R26

Reaction centers from Rhodobacter sphaeroides have been modified by treatment with sodium borohydride similar to the original procedure [Ditson et al., Biochim. Biophys. Acta 766, 623 (1984)], and investigated spectroscopically and by gel electrophoresis. (1) Low temperature (1.2 K) absorption, fluorescence, absorption- and fluorescence-detected ODMR, and microwave-induced singlet-triplet absorption difference spectra (MIA) suggest that the treatment produces a spectroscopically homogeneous preparation with one of the ‘additional’ bacteriochlorophylls being removed. The modification does not alter the zero field splitting parameters of the primary donor triplet (TP870). (2) From the circular dichroism and Raman resonance spectra in the1500–1800 cm-1 region, the removed pigment is assigned to BchlM, e.g. the "extra" Bchl on the "inactive" M-branch. (3) A strong coupling among all pigment molecules is deduced from the circular dichroism spectra, because pronounced band-shifts and/or intensity changes occur in the spectral components assigned to all pigments. This is supported by distinct differences among the MIA spectra of untreated and modified reaction centers, as well as by Raman resonance. (4) The modification is accompanied by partial proteolytic cleavage of the M-subunit. The preparation is thus spectroscopically homogeneous, but biochemically heterogenous

ESR Study of (C_5H_{12}N)_2CuBr_4

ESR studies at 9.27, 95.4, and 289.7 GHz have been performed on (C$_5$H$_{12}$N)$_2$CuBr$_4$ down to 3.7 K. The 9.27 GHz data were acquired with a single crystal and do not indicate the presence of any structural transitions. The high frequency data were collected with a polycrystalline sample and resolved two absorbances, consistent with two crystallographic orientations of the magnetic sites and with earlier ESR studies performed at 300 K. Below $B_{C1}=6.6$ T, our data confirm the presence of a spin singlet ground state.Comment: 2 pages, 4 figs., submitted 23rd International Conference on Low Temperature Physics (LT-23), Aug. 200

Microwave properties of Nd_0.5Sr_0.5MnO_3: a key role of the (x^2-y^2)-orbital effects

Transmittance of the colossal magnetoresistive compound Nd_0.5Sr_0.5MnO_3 showing metal-insulator phase transition has been studied by means of the submm- and mm-wavelength band spectroscopy. An unusually high transparency of the material provided direct evidence for the significant suppression of the coherent Drude weight in the ferromagnetic metallic state. Melting of the A-type antiferromagnetic states has been found to be responsible for a considerable increase in the microwave transmission, which was observed at the transition from the insulating to the metallic phase induced by magnetic field or temperature. This investigation confirmed a dominant role of the (x^2-y^2)-orbital degree of freedom in the low-energy optical properties of Nd_0.5Sr_0.5MnO_3 and other doped manganites with planar (x^2-y^2)-orbital order, as predicted theoretically. The results are discussed in terms of the orbital-liquid concept.Comment: 8 pages, 3 figure

The Flow of Family Transitions of Australian Families

Family structure disruption has been linked to negative child educational and health outcomes (Perales et al. 2016). Australia has relatively stable families, but income disparities between Australians are widening, and single-parent families make up a large proportion of families living in poverty. Cohabitation is also common in Australia with approximately three-quarters of marriages preceded by cohabitation. If substantial family structure churning affects Australian children this may expose a need for special policy interventions aimed at family creation and dissolution to ameliorate the negative effects of such stressful experiences. To highlight family structures and transitions Australian children experience, we use Sankey flow diagrams charting data from ‘Growing Up in Australia: The Longitudinal Study of Australian Children (LSAC)’ (Gilding 2001). We track children from birth to 11 years old using waves 1-6 and population weights to represent 183,521 children born into Australian families

Arrival of Paleo-Indians to the Southern Cone of South America: New Clues from Mitogenomes

With analyses of entire mitogenomes, studies of Native American mitochondrial DNA (mtDNA) variation have entered the final phase of phylogenetic refinement: the dissection of the founding haplogroups into clades that arose in America during and after human arrival and spread. Ages and geographic distributions of these clades could provide novel clues on the colonization processes of the different regions of the double continent. As for the Southern Cone of South America, this approach has recently allowed the identification of two local clades (D1g and D1j) whose age estimates agree with the dating of the earliest archaeological sites in South America, indicating that Paleo-Indians might have reached that region from Beringia in less than 2000 years. In this study, we sequenced 46 mitogenomes belonging to two additional clades, termed B2i2 (former B2l) and C1b13, which were recently identified on the basis of mtDNA control-region data and whose geographical distributions appear to be restricted to Chile and Argentina. We confirm that their mutational motifs most likely arose in the Southern Cone region. However, the age estimate for B2i2 and C1b13 (11–13,000 years) appears to be younger than those of other local clades. The difference could reflect the different evolutionary origins of the distinct South American-specific sub-haplogroups, with some being already present, at different times and locations, at the very front of the expansion wave in South America, and others originating later in situ, when the tribalization process had already begun. A delayed origin of a few thousand years in one of the locally derived populations, possibly in the central part of Chile, would have limited the geographical and ethnic diffusion of B2i2 and explain the present-day occurrence that appears to be mainly confined to the Tehuelche and Araucanian-speaking grou

Enhanced abundances in three large-diameter mixed-morphology supernova remnants

We present an X-ray study of three mixed-morphology supernova remnants (SNRs), HB 21, CTB 1 and HB 3, using archival ASCA and ROSAT data. These data are complemented by archival Chandra X-ray Observatory data for CTB 1 and XMM-Newton X-ray Observatory data for HB 3. The spectra from HB 21 and HB 3 are well-described with a single-temperature thermal plasma in ionization equilibrium, while a two-temperature thermal plasma is found in CTB 1. We found enhanced abundances in all three SNRs. The elemental abundance of Mg is clearly enhanced in CTB 1, while HB 21 has enhanced abundances of Si and S. The situation is not so clear in HB 3 -- the plasma in this SNR either has significantly enhanced abundances of O, Ne and Mg, or it has marginally enhanced abundances of Mg and under-abundant Fe. We discuss the plausibility of mixed-morphology SNR models for the three SNRs and the presence of enhanced abundances. We revise a list of MM SNRs and their properties, compare the three SNRs studied here with other members of this class, and discuss the presence of enhanced elemental abundances in MM SNRs. We also report the ASCA detection of a compact source in the southern part of HB 3. The source spectrum is consistent with a power law with a photon index of ~2.7, and an unabsorbed X-ray flux of ~10^{-12} erg/cm^2/s in the 0.5--10.0 keV band. The column density towards this source differs from that towards the SNR, and it is therefore unlikely they are related.Comment: 26 pages, 15 figures, revised version (minor changes), accepted for publication in ApJ (10 Aug 2006

Re-identification of G35.6-0.4 as a supernova remnant

G35.6-0.4 is an extended radio source in the Galactic plane which has previously been identified as either a supernova remnant or an HII region. Observations from the VLA Galactic Plane Survey at 1.4 GHz with a resolution of 1 arcmin allow the extent of G35.6-0.4 to be defined for the first time. Comparison with other radio survey observations show that this source has a non-thermal spectral index, with alpha -0.47 +/- 0.07. G35.6-0.4 does not have obvious associated infra-red emission, so it is identified as a Galactic supernova remnant, not an HII region. It is approximately 15 x 11 arcmin**2 in extent, showing partial limb brightening.Comment: 4 pages, accepted by MNRA