33 research outputs found
INVESTIGA脟脙O POR PLANEJAMENTO EXPERIMENTAL DA INFLU脢NCIA DA AGITA脟脙O E AERA脟脙O NO PROCESSO PRODUTIVO DE XANTANA POR Xanthomonas arboricola pv pruni
Identification of pesticide-degrading Pseudomonas strains as poly-尾-hydroxybutyrate producers
Polyhydroxybutyrate (PHB) is an ecological promising substitute for polypropylene because it is biocompatible, biodegradable and can be produced by renewable sources. This study investigated PHB accumulation on pesticide-degrading Pseudomonas. 14 strains of pesticide-degrading Pseudomonas isolated from subtropical lowland soil in southern Brazil were analyzed using optical microscopy. Lipid inclusions were identified in four strains, and three of them, which degraded the pesticide carbofuran, had extensive granules accumulation which was detected by transmission electron microscopy. These strains were cultivated in a shaker at 28掳C and the polymer was characterized by Fourier transform infrared (FTIR) spectroscopy. Strain CMM43 had the best accumulation after 48 h. The biopolymer was identified as poly-尾-hydroxybutyrate.Key words: Polyhydroxybutyrate (PHB), pesticide-degrading Pseudomonas sp., microscopy, subtropical lowland soil
Segmentation and Classification of Cine-MR Images Using Fully Convolutional Networks and Handcrafted Features
Three-dimensional cine-MRI is of crucial importance for assessing the cardiac
function. Features that describe the anatomy and function of cardiac structures
(e.g. Left Ventricle (LV), Right Ventricle (RV), and Myocardium(MC)) are known
to have significant diagnostic value and can be computed from 3D cine-MR
images. However, these features require precise segmentation of cardiac
structures. Among the fully automated segmentation methods, Fully Convolutional
Networks (FCN) with Skip Connections have shown robustness in medical
segmentation problems. In this study, we develop a complete pipeline for
classification of subjects with cardiac conditions based on 3D cine-MRI. For
the segmentation task, we develop a 2D FCN and introduce Parallel Paths (PP) as
a way to exploit the 3D information of the cine-MR image. For the
classification task, 125 features were extracted from the segmented structures,
describing their anatomy and function. Next, a two-stage pipeline for feature
selection using the LASSO method is developed. A subset of 20 features is
selected for classification. Each subject is classified using an ensemble of
Logistic Regression, Multi-Layer Perceptron, and Support Vector Machine
classifiers through majority voting. The Dice Coefficient for segmentation was
0.95+-0.03, 0.89+-0.13, and 0.90+-0.03 for LV, RV, and MC respectively. The
8-fold cross validation accuracy for the classification task was 95.05% and
92.77% based on ground truth and the proposed methods segmentations
respectively. The results show that the PPs increase the segmentation accuracy,
by exploiting the spatial relations. Moreover, the classification algorithm and
the features showed discriminability while keeping the sensitivity to
segmentation error as low as possible.Comment: 9 pages, 3 figures, 2 tables. Was accepted to the ACDC challenge,
MICCAI 2017 (not attended
Influ锚ncia de diferentes extratos de levedura (insumos) no crescimento celular de Xanthomonas arboricola pv pruni cepa 101/ Influence of different yeast extracts (inputs) on the cell growth of Xanthomonas arboricola pv pruni strain 101
Xantana 茅 um heteropolissacar铆deo produzido por bact茅rias do g锚nero Xanthomonas. Esse biopol铆mero desempenha diversas fun莽玫es de grande import芒ncia para ind煤strias de variados setores. O processo de produ莽茫o de xantana 茅 realizado em duas etapas: crescimento celular e produ莽茫o do biopol铆mero, e ambas s茫o importantes para a obten莽茫o de xantana com rendimento e qualidade satisfat贸rios, os quais s茫o influenciados por diferentes fatores, dentre eles os meios de cultivo utilizados. Objetivou-se avaliar o crescimento bacteriano de Xanthomonas arboricola pv pruni cepa 101 em meio de cultivo complexo YM adicionado de diferentes extratos de levedura, sendo um tradicional - controle (1) - e outros alternativos de menor custo - 560PW (2), 810PW (3), 845MG (4), 851MG (5), 861PW (6) - e relacionar com o teor de nitrog锚nio fornecido. Avaliou-se o crescimento celular (UFC.mL-1) e o teor de nitrog锚nio (mg.dL-1) nos tempos 24 h e em 0 e 24 h de crescimento celular, respectivamente. N茫o observou-se diferen莽a estat铆stica entre a concentra莽茫o celular final obtida com extrato controle e os extratos alternativos. A concentra莽茫o celular final variou entre 3,1 脳 1010 a 5,2 脳 1010 UFC.mL-1. O uso dos extratos de levedura 4, 6 e o controle resultaram em maior disponibilidade de nitrog锚nio no meio de cultivo no tempo inicial e ao final, e t锚m potencial para propiciar um maior crescimento celular. Observou-se aumento no teor de nitrog锚nio no tempo final, provavelmente devido 脿 hidr贸lise celular parcial. 聽O teor inicial de nitrog锚nio variou de 9,11 a 12,04 e o final de 31,41 a 47,51 mg.dL-1, respectivamente. Os diferentes extratos de levedura avaliados s茫o substitutos adequados para o crescimento celular da bact茅ria X. arboricola pv pruni cepa 101, e provavelmente para outras cepas da esp茅cie, com resultados equivalentes ao obtido com o extrato controle
MODIFICA脟脮ES QU脥MICAS EM XANTANA PRODUZIDA POR Xanthomonas arboricola pv pruni PROMOVIDAS POR TRATAMENTO T脡RMICO
Influencia de la agitaci贸n y la aireaci贸n en la producci贸n de xantano por Xanthomonas campestris pv. pruni cepa 101
Se evalu贸 la producci贸n, viscosidad y composici贸n qu铆mica del xantano sintetizado por la bacteria Xanthomonas campestris pv. pruni cepa 101 en un fermentador. Durante el proceso se control贸 el pH y se determinaron el coeficiente de transferencia de masa de ox铆geno (kLa) y la producci贸n de masa celular seca. Los cultivos se realizaron en un fermentador de 3 l variando la aireaci贸n y la agitaci贸n, en las siguientes condiciones: (A) 300 rpm, 3 vvm y (B) 200 rpm, 2 vvm; a 28 掳C. Nuestros resultados mostraron que la producci贸n de goma fue dependiente del kLa, con un rendimiento m谩ximo de 8,15 g/l a 300 rpm y 3 vvm a las 54 h de fermentaci贸n, kLa de 21,4/h, mientras que la producci贸n de biomasa no se afect贸. Todas las soluciones acuosas de xantano al 3% (m/v) sintetizadas presentaron comportamiento pseudopl谩stico. La mayor viscosidad se alcanz贸 en la condici贸n de aireaci贸n/agitaci贸n m谩s intensa. Todas las muestras de xantano conten铆an glucosa, manosa, ramnosa y 谩cido glucur贸nico como constituyentes principales. La mayor tasa de agitaci贸n y aireaci贸n utilizada en la condici贸n A (300 rpm y 3 vvm) influy贸 favorablemente en el rendimiento y la viscosidad del xantano producido por la bacteria X. campestris pv. pruni 101 a diferentes tiempos de fermentaci贸n.Production, viscosity, and chemical composition of xanthan synthesized by bacterium Xanthomonas campestris pv pruni strain 101 were evaluated in bioreactor systems. During the process, the volumetric oxygen mass transfer coefficient (kLa) and the biomass were determined and the pH was monitored. The cultures were grown in a 3 l bioreactor, with aeration and agitation varying as follows: conditions (A) 300 rpm, 3 vvm and (B) 200 rpm, 2 vvm, at 28 掳C. Our results showed that gum production was dependent on kLa, with a maximum yield of 8.15 g/l at 300 rpm, 3 vvm, 54 h of fermentation, kLa 21.4/h, while biomass was not affected. All aqueous solutions of 3% (w/v) xanthans synthesized showed a pseudoplastic behavior. The highest viscosity was reached under the strongest aeration/agitation conditions. All xanthan samples contained glucose, mannose, rhamnose, and glucuronic acid as their main components. The highest agitation and aeration rates used under condition A (300 rpm and 3 vvm) favorably influenced the yield and viscosity of the xanthan produced by bacterium X. campestris pv pruni 101 at different fermentation times
Influencia de la agitaci贸n y la aireaci贸n en la producci贸n de xantano por Xanthomonas campestris pv. pruni cepa 101
Se evalu贸 la producci贸n, viscosidad y composici贸n qu铆mica del xantano sintetizado por la bacteria Xanthomonas campestris pv. pruni cepa 101 en un fermentador. Durante el proceso se control贸 el pH y se determinaron el coeficiente de transferencia de masa de ox铆geno (kLa) y la producci贸n de masa celular seca. Los cultivos se realizaron en un fermentador de 3 l variando la aireaci贸n y la agitaci贸n, en las siguientes condiciones: (A) 300 rpm, 3 vvm y (B) 200 rpm, 2 vvm; a 28 掳C. Nuestros resultados mostraron que la producci贸n de goma fue dependiente del kLa, con un rendimiento m谩ximo de 8,15 g/l a 300 rpm y 3 vvm a las 54 h de fermentaci贸n, kLa de 21,4/h, mientras que la producci贸n de biomasa no se afect贸. Todas las soluciones acuosas de xantano al 3% (m/v) sintetizadas presentaron comportamiento pseudopl谩stico. La mayor viscosidad se alcanz贸 en la condici贸n de aireaci贸n/agitaci贸n m谩s intensa. Todas las muestras de xantano conten铆an glucosa, manosa, ramnosa y 谩cido glucur贸nico como constituyentes principales. La mayor tasa de agitaci贸n y aireaci贸n utilizada en la condici贸n A (300 rpm y 3 vvm) influy贸 favorablemente en el rendimiento y la viscosidad del xantano producido por la bacteria X. campestris pv. pruni 101 a diferentes tiempos de fermentaci贸n.Production, viscosity, and chemical composition of xanthan synthesized by bacterium Xanthomonas campestris pv pruni strain 101 were evaluated in bioreactor systems. During the process, the volumetric oxygen mass transfer coefficient (kLa) and the biomass were determined and the pH was monitored. The cultures were grown in a 3 l bioreactor, with aeration and agitation varying as follows: conditions (A) 300 rpm, 3 vvm and (B) 200 rpm, 2 vvm, at 28 掳C. Our results showed that gum production was dependent on kLa, with a maximum yield of 8.15 g/l at 300 rpm, 3 vvm, 54 h of fermentation, kLa 21.4/h, while biomass was not affected. All aqueous solutions of 3% (w/v) xanthans synthesized showed a pseudoplastic behavior. The highest viscosity was reached under the strongest aeration/agitation conditions. All xanthan samples contained glucose, mannose, rhamnose, and glucuronic acid as their main components. The highest agitation and aeration rates used under condition A (300 rpm and 3 vvm) favorably influenced the yield and viscosity of the xanthan produced by bacterium X. campestris pv pruni 101 at different fermentation times
ELABORA脟脙O DE BEBIDA TIPO N脡CTAR DE GRAVIOLA ADO脟ADA COM MEL DE Apis mellifera
The objective of this st udy was to prepare drinks type soursop nectars sweetened with honey from Apis mellifera , by use of experimental planning, varying the concentrations of pulp and soluble solids content and evaluate the effect of these in the physical and chemical characteristics of processed drinks. Were used a factorial planning 2 2 with three experiments in central point resulting in seven experiments. The soursop pulp was diluted with potable water to obtain products in the concentrations of 25, 30 and 35% (w/w) of pulp. The correction of soluble solids to 11, 12 and 13 掳Brix was made using honey from A. mellifera . It was ob- served that all formulations of drinks type soursop nectar sweetened with honey were in accordance with bra- zilian legislation. The mathematical equations fitted to the data of titratable acidity, total sugar, ratio, brightness and intensity of yellow were significant, and only the models fitted to the values of titratable acidity, total sug- ars and ratio were predictive. The responses of titratable acidity, total sugar, ratio, brightness and yellow color intensity were influenced by factors pulp concentration and total soluble solids content, wherein the concentra- tion of soursop pulp had greater influence on the acidity and the ratio and the content final total soluble solids of the beverages, modified by the addition of honey, showed greater influence on the content of total sugars and lightness values. The yellow intensity response showed influences of both factors