Oregon Lake Watch, 2014 Annual Report

The Oregon Lake Watch (OLW) volunteer monitoring program is now in its second year of operation, continuing its focus on early detection of aquatic invasive species (AIS), with a secondary focus on lake water quality. The aquatic invasive species chosen as Watch List priorities for the OLW are based on the Oregon Department of Agriculture’s Noxious Weed List (Oregon Department of Agriculture Noxious Weed Control Program 2014) as well as professional judgement (more information can be found in Oregon Lake Watch Final Report 2014). This report summarizes the data collected by 15 of 24 OLW volunteers over 2014, across 110 sampling events and 20 Oregon lakes. Data collected by trained volunteers includes sightings of both native and aquatic invasive plants and animals, as well as recordings of lake temperature profiles, and Secchi depth, a measure of clarity. Of the 16 original water bodies surveyed in OLW’s first year (2013), 12 were surveyed again in 2014, and 8 are new waterbodies. Long-term monitoring of these lakes provides valuable information over time, and expanding the number of lakes monitored, especially in the case of monitoring for invasive species, means that more potentially harmful invasions can be stopped sooner. Ideally, the OLW will continue to monitor these current lakes, and expand their volunteer network to cover more waterbodies across Oregon as this program continues to grow

Oregon Lake Watch, 2015 Annual Report

The Oregon Lake Watch (OLW) volunteer monitoring program completed its third year of operation during 2015 with a focus on early detection of aquatic invasive species (AIS) and water quality status and trend assessments for the better management of Oregon’s lakes and reservoirs. AIS surveys emphasized the detection of Watch List aquatic plant and species that can have a negative impacts on the beneficial uses of our waterbodies. Water transparency and temperature measurements were used to track the condition of volunteers\u27 lakes. Thirty-one Oregon lakes have been surveyed during the OLW’s three years, 16 of which were surveyed during 2015. Seven lakes have been surveyed all three years and seven more have been surveyed two of the three years. Four Watch List plant and one animal species were found during 2015. Eurasian watermilfoil (Myriophyllum spicatum) was found in three lakes, Brazilian elodea (Egeria densa) and curly leaf pondweed (Potamogeton crispus) were each found in two lakes, and yellow flag iris (Iris pseudacorus) was found in one lake. Asian clams (Corbicula fluminea) were found in one lake. A diverse array of native plants were found along with native signal crayfish (Pacifasticus leniusculus). Water quality ranged from eutrophic to oligotrophic conditions based on water transparency and water temperatures ranged as high as 28°C (82°F). More years of data collection are necessary to track water quality trends

Physical, Chemical and Biological Assessment of Yoncalla Log Ponds

The Yoncalla Log Ponds are a series of four ponds located in within the city limits of the town in Yoncalla in northern Douglas County, Oregon. The ponds were created in stages between the late 1930s and the 1950s and used for log storage through the 1970s (North Douglas Betterment 2014). North Douglas Betterment purchased the land surrounding the ponds and contracted with the Center for Lakes and Reservoirs at Portland State University to assess the current morphometry, vegetation and water quality status of the ponds and provide recommendations for management of the aquatic vegetation. The scope of this document covers the current status assessment of the ponds using data collected from June 2013 through May 2014

Zooplankton recovery from a whole-lake disturbance: Examining roles of abiotic factors, biotic interactions, and traits

Community assembly following disturbance is a key process in determining the composition and function of the future community. However, replicated studies of community assembly at whole-ecosystem scales are rare. Here, we describe a series of whole-lake experiments, in which the recovery of zooplankton communities was tracked following an ecosystem-scale disturbance, that is, application of the piscicide, rotenone. Using a before-after-control-impact design, 14 lakes in eastern Washington were studied: Seven lakes were treated with rotenone, while seven lakes acted as reference systems. Each lake was monitored up to 6 months before and 1–2 years after the rotenone treatments. Zooplankton samples and environmental measurements were collected approximately monthly from each lake. Community responses following disturbance were assessed using metrics of abundance, diversity, and community composition, as well as taxonomic group abundance. Zooplankton recovery was also assessed using species traits related to habitat, feeding mode, trophic level, body size, and life history. In addition to patterns of recovery, potential mechanisms were explored relating to abiotic conditions, biotic interactions, and traits. There were steep declines in the abundance (average across years: 99%) and diversity (average across years: 75%) of the zooplankton community following rotenone treatment. Although abundance had recovered by the second year of the study, community diversity had not fully recovered after 2 years. Communities from rotenone lakes appeared to be compositionally recovered within about 8 months following disturbance. Cyclopoid copepods were typically the first group to recover and remained dominant for a few months, whereas cladocerans recovered more slowly, typically within ~6–7 months following rotenone. Calanoid copepods were not fully recovered 2 years after rotenone treatment. Traits related to body size and feeding mode were associated with the zooplankton communities following rotenone treatment. We failed to observe significant spatial synchrony in recovery patterns of zooplankton across lakes, though we did observe significant synchrony of zooplankton taxonomic groups within lakes. These findings suggest that traits related to ecological function, and to a lesser extent, biotic, and abiotic factors, as well as characteristics of the disturbance itself, may be important in helping to understand recovery processes

Fish Species Introductions Provide Novel Insights into the Patterns and Drivers of Phylogenetic Structure in Freshwaters

Despite long-standing interest of terrestrial ecologists, freshwater ecosystems are a fertile, yet unappreciated, testing ground for applying community phylogenetics to uncover mechanisms of species assembly. We quantify phylogenetic clustering and overdispersion of native and non-native fishes of a large river basin in the American Southwest to test for the mechanisms (environmental filtering versus competitive exclusion) and spatial scales influencing community structure. Contrary to expectations, non-native species were phylogenetically clustered and related to natural environmental conditions, whereas native species were not phylogenetically structured, likely reflecting human-related changes to the basin. The species that are most invasive (in terms of ecological impacts) tended to be the most phylogenetically divergent from natives across watersheds, but not within watersheds, supporting the hypothesis that Darwin\u27s naturalization conundrum is driven by the spatial scale. Phylogenetic distinctiveness may facilitate non-native establishment at regional scales, but environmental filtering restricts local membership to closely related species with physiological tolerances for current environments. By contrast, native species may have been phylogenetically clustered in historical times, but species loss from contemporary populations by anthropogenic activities has likely shaped the phylogenetic signal. Our study implies that fundamental mechanisms of community assembly have changed, with fundamental consequences for the biogeography of both native and non-native species

Chromophoric Dissolved Organic Matter and Dissolved Organic Carbon in Lakes Across an Elevational Gradient From the Mountains to the Sea

Dissolved organic matter (DOM) in lakes across elevation gradients is a complex function of topography, climate, vegetation coverage, land use, and lake properties. To examine sources and processing of DOM from sea level to mountain lakes (3–1,574 m), we measured dissolved organic carbon (DOC) concentrations and chromophoric dissolved organic matter (CDOM) optical properties, lake characteristics, and water quality parameters in 62 freshwater lakes in the Pacific Northwest, USA. Higher elevation lakes had lower DOC concentrations and absorbance. These lakes had higher forest cover and minimal wetlands in their watershed, in addition to low nutrients, water temperatures, and chlorophyll a in the lake itself. Two humic-like and one protein-like fluorescent component were identified from excitation-emission matrix spectroscopy. The index of recent autochthonous contribution (BIX), fluorescence index (FIX), and SR optical indices showed that most lakes were dominated by terrestrially derived material. The humification index (HIX) and specific ultra-violet absorbance (SUVA254) were consistent with more aromatic humic CDOM at lower elevations. The lower fluorescence of humic-like components at higher elevation was attributed to lower inputs from vegetation. The relative contribution of the protein-like component increased at higher elevation. This may be due to reduced allochthonous terrestrial inputs relative to in situ production of autochthonous material or increased photochemical/biological degradation of allochthonous material. Differences in optical characteristics associated with the amount and source of CDOM were observed across the elevational gradient. These differences were driven by characteristics at both within-lake and watershed scales

Supporting Information for Chromophoric Dissolved Organic Matter (CDOM) Across an Elevational Gradient from Sea Level to Mountain Lakes in the Pacific Northwest

This dataset is in support of Juetten et al., which has been submitted to the Journal of Geophysical Research Biogeosciences for consideration for publication. Chromophoric dissolved organic matter (CDOM) in lakes across elevation gradients is a complex function of topography, climate, vegetation coverage, land use, and lake properties. To examine sources and processing of CDOM from sea level to mountain lakes (3 to 1574 m), we measured CDOM optical properties, lake characteristics, and water quality parameters in 62 freshwater lakes in the Pacific Northwest, USA. Higher elevation lakes had lower dissolved organic carbon (DOC) and absorbance. These lakes had higher forest cover, minimal wetlands or crops, low nutrients, cooler water temperatures, and low chlorophyll a. Two humic-like and one protein-like fluorescent component were identified from excitation-emission matrix spectroscopy (EEMs). The index of recent autochthonous contribution (BIX), fluorescence index (FIX) and SR optical indices indicated that most lakes were dominated by terrestrially-derived material. The humification index (HIX) and specific ultra-violet absorbance (SUVA) were consistent with more aromatic humic CDOM at lower elevations. Fluorescence of humic-like components was lower at higher elevation, indicating reduced amounts of CDOM, attributed to lower inputs from vegetation. The relative contribution of the protein-like component increased at higher elevation, because allochthonous terrestrial inputs are reduced relative to in situ production of autochthonous material or higher photochemical and biological degradation of allochthonous material. Differences in optical characteristics associated with CDOM levels and some characteristics associated with CDOM source and quality were observed across the elevational gradient. These differences were driven by characteristics at both within-lake and watershed scales, suggesting a more complete understanding of CDOM patterns can be gained by examining factors at multiple spatial scales. The dataset available below is supplementary information to the paper in a Word file format. This includes: a figure corresponding to the RDA Figure 6 in the manuscript giving the lake names; a table giving sampling dates, elevations, locations and watershed characteristics for the lakes; a table comparing water quality parameters for deep vs, surface water samples from Lake Padden in 2011; and a table giving the measured water quality parameters for the lakes from summer 2018

Water Supply, Demand, and Quality Indicators for Assessing the Spatial Distribution of Water Resource Vulnerability in the Columbia River Basin

We investigated water resource vulnerability in the US portion of the Columbia River basin (CRB) using multiple indicators representing water supply, water demand, and water quality. Based on the US county scale, spatial analysis was conducted using various biophysical and socio-economic indicators that control water vulnerability. Water supply vulnerability and water demand vulnerability exhibited a similar spatial clustering of hotspots in areas where agricultural lands and variability of precipitation were high but dam storage capacity was low. The hotspots of water quality vulnerability were clustered around the main stem of the Columbia River where major population and agricultural centres are located. This multiple equal weight indicator approach confirmed that different drivers were associated with different vulnerability maps in the sub-basins of the CRB. Water quality variables are more important than water supply and water demand variables in the Willamette River basin, whereas water supply and demand variables are more important than water quality variables in the Upper Snake and Upper Columbia River basins. This result suggests that current water resources management and practices drive much of the vulnerability within the study area. The analysis suggests the need for increased coordination of water management across multiple levels of water governance to reduce water resource vulnerability in the CRB and a potentially different weighting scheme that explicitly takes into account the input of various water stakeholders

Phase 1 Trials of rVSV Ebola Vaccine in Africa and Europe.

BACKGROUND: The replication-competent recombinant vesicular stomatitis virus (rVSV)-based vaccine expressing a Zaire ebolavirus (ZEBOV) glycoprotein was selected for rapid safety and immunogenicity testing before its use in West Africa. METHODS: We performed three open-label, dose-escalation phase 1 trials and one randomized, double-blind, controlled phase 1 trial to assess the safety, side-effect profile, and immunogenicity of rVSV-ZEBOV at various doses in 158 healthy adults in Europe and Africa. All participants were injected with doses of vaccine ranging from 300,000 to 50 million plaque-forming units (PFU) or placebo. RESULTS: No serious vaccine-related adverse events were reported. Mild-to-moderate early-onset reactogenicity was frequent but transient (median, 1 day). Fever was observed in up to 30% of vaccinees. Vaccine viremia was detected within 3 days in 123 of the 130 participants (95%) receiving 3 million PFU or more; rVSV was not detected in saliva or urine. In the second week after injection, arthritis affecting one to four joints developed in 11 of 51 participants (22%) in Geneva, with pain lasting a median of 8 days (interquartile range, 4 to 87); 2 self-limited cases occurred in 60 participants (3%) in Hamburg, Germany, and Kilifi, Kenya. The virus was identified in one synovial-fluid aspirate and in skin vesicles of 2 other vaccinees, showing peripheral viral replication in the second week after immunization. ZEBOV-glycoprotein-specific antibody responses were detected in all the participants, with similar glycoprotein-binding antibody titers but significantly higher neutralizing antibody titers at higher doses. Glycoprotein-binding antibody titers were sustained through 180 days in all participants. CONCLUSIONS: In these studies, rVSV-ZEBOV was reactogenic but immunogenic after a single dose and warrants further evaluation for safety and efficacy. (Funded by the Wellcome Trust and others; ClinicalTrials.gov numbers, NCT02283099, NCT02287480, and NCT02296983; Pan African Clinical Trials Registry number, PACTR201411000919191.)

OSMB Final Report: Task 6. Tenmile Lake Boat Wash Effectiveness Monitoring

Public awareness of aquatic invasive species and proper boat cleaning procedures may prove to be beneficial in reducing the transport and establishment of aquatic invasive species like New Zealand mud snails and zebra and quagga mussels as well as hydrilla and Eurasian watermilfoil. The primary objectives of this research project were to observe the use and determine the efficacy of a public boat wash station as well as increase the public’s awareness of proper boat cleaning procedures and aquatic invasive species. The Tenmile Lakes Basin Partnership, Oregon State Marine Board, and the United States Forest Service (USFS) have undertaken an initiative to increase public awareness of invasive species and proper cleaning procedures by building a boat wash station at the Tenmile Lake public boat launch. In the summer of 2012, 199 qualitative human subject surveys were administered at Tenmile Lake in Lakeside, Oregon to boaters on their boating habits and knowledge of invasive species prior to building the boat wash (“pre-boat wash”). An extension of the 2012 study was conducted in the summer of 2013 on 200 boaters after the completion of the Tenmile Lake boat wash station (“post-boat wash”). Comparisons were made on the observations and answers of boaters prior to and after the boat wash installation. Of the boaters surveyed in the pre-boat wash field season, 75.9% of boaters claimed they would use a boat wash station at Tenmile Lake. The actual use of the boat wash station based on observations made by the field researcher during the post-boat wash field season indicated only 38.5% of surveyed boaters used the station. Furthermore, more than 20% of boaters could not verbally identify an invasive species in either field season when asked their awareness of aquatic invasive species. However, the majority of boaters surveyed at Tenmile Lake in the pre-boat wash field season (63.3%) and post-boat wash field season (66%) were aware of the phrase “Clean, Drain, Dry”. These results identify a disconnect in what boaters say and what boaters do and the knowledge gaps boaters have on aquatic invasive species, but these results also identify a growing awareness of proper boat cleaning procedures