Sarcomas primarios de hueso: estudio por citometría estática mediante análisis digital de imagen

Se presenta un estudio morfométrico y densitométrico mediante análisis digital de imagen de una serie de 50 tumores óseos malignos (32 osteosarcomas, 12 condrosarcomas y 6 histiocitomas fibrosos malignos de hueso), con el fin de evaluar la utilidad de la técnica para establecer el grado y el pronóstico de estas neoplasias. Las variables morfométricas y la disposición de la cromatina guardan una estrecha relación con el grado histológico (Spearman; p < 0,05) y muestran diferencias estadísticamente significativas entre los tumores considerados de alto y bajo grado en base a su presentación y evolución clínica. La ploidía estimada por densitometría óptica se comporta como un factor pronóstico independiente en el análisis multivariante de la serie de tumores de alto grado (COX; p < 0,001). La citometría estática, aunque más lenta e imprecisa que la citometría de flujo en la determinación de los picos de ploidía, permite analizar el modelo de cromatina y contribuye objetivamente al establecimiento del grado biológico de los tumores óseos.A densitometry and morphometric assessment by static image cytometry of 50 malignant bone tumors (32 osteosarcomas, 12 chondrosarcomas, and 6 malignant fibrous histiocytomas of bone) was performed in order to assess the validity of the technic in the pathobiological and prognostic evaluation of these neoplasms. A strong correlation between the morphometric features, cromatine distribution and histologic grade was found (Spearman; p < 0.05). These parameters are also statistically different for hight and low grade tumors in clinical staging. The stimation of DNA ploidy by optic densitometry is a significant prognostic factor in the multivariant analysis for the high grade tumors (COX; p < 0.001). Although Flow cytometry is a better procedure to evaluate DNA ploidy peaks, our study reveals static image cytometry as a useful technic for determine the cromatine model and objective assessment of the biological grade of bone neoplasms

Annular erythematous papules in the neckline.

A 45-year-old woman with personal history of hypertension presented with an erythematous lesion in the neckline for a year and with a progressive growth. A physical examination revealed an annular lesion with erythematous papules in the edge. Histological exam showed phagocytosis of elastic fibers by multinucleated cells compatible with annular elastolytic giant-cell granuloma. The patient did not present any other associated systemic manifestation. Treatment with tacrolimus 0.1 percent ointment was prescribed with a very good response after two months.YesUna mujer de 45 años de edad con antecedentes personales de hipertensión presenta una lesión eritematosa en el cuello durante un año y con un crecimiento progresivo. El examen físico reveló una anular lesión con pápulas eritematosas en el borde. Examen histológico reveló la fagocitosis de las fibras elásticas de las células multinucleadas compatible con anular elastolíticas granuloma de células gigantes. El paciente no presentó ninguna otra manifestación sistémica asociada. El tratamiento con pomada de tacrolimus 0,1 por ciento fue recetado con una muy buena respuesta después de dos meses

ILDS Newsletter No. 24

On the occasion of the 70th annual meeting of the American Academy of Dermatology in San Diego the ILDS Executive Committee hosted a cocktail reception in the evening of 18 March. The reception, held at the Marriott Hotel on an evening of several competing attractions, was well attended by friends of the ILDS, who stopped by for a drink and a chat. Jean Bolognia and Chris Griffiths acted as official ILDS meeters and greeters for the guests. Our president, Wolfram Sterry gave a short speech of welcome and provided an update for those present on the recent achievements of and plans for the ILDS. The announcement of the Berlin summit was particularly well received by our member societies. Jerry Shapiro then took the floor to apprise us of the arrangements for the Vancouver World Congress, these too were greeted with enthusiasm..