354 research outputs found

    Unpacking the client(s): constructions, positions and client–consultant dynamics

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    Research on management consultancy usually emphasizes the role and perspective of the consultants. Whilst important, consultants are only one element in a dynamic relationship involving both consultants and their clients. In much of the literature, the client is neglected, or is assumed to represent a distinct, immutable entity. In this paper, we argue that the client organisation is not uniform but is instead (like organisations generally) a more or less heterogeneous assemblage of actors, interests and inclinations involved in multiple and varied ways in consultancy projects. This paper draws upon three empirical cases and emphasizes three key aspects of clients in the context of consultancy projects: (a) client diversity, including, but not limited to diversity arising solely from (pre-)structured contact relations and interests; (b) processes of constructing ‘the client’ (including negotiation, conflict, and reconstruction) and the client identities which are thereby produced; and (c) the dynamics of client–consultant relations and how these influence the construction of multiple and perhaps contested client positions and identities

    Advances in 3D single particle localization microscopy

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    The spatial resolution of conventional optical microscopy is limited by diffraction to transverse and axial resolutions of about 250 nm, but localization of point sources, such as single molecules or fluorescent beads, can be achieved with a precision of 10 nm or better in each direction. Traditional approaches to localization microscopy in two dimensions enable high precision only for a thin in-focus layer that is typically much less than the depth of a cell. This precludes, for example, super-resolution microscopy of extended three-dimensional biological structures or mapping of blood velocity throughout a useful depth of vasculature. Several techniques have been reported recently for localization microscopy in three dimensions over an extended depth range. We describe the principles of operation and typical applications of the most promising 3D localization microscopy techniques and provide a comparison of the attainable precision for each technique in terms of the Cramér-Rao lower bound for high-resolution imaging

    A 200 Year Sub-Annual Record of Sulfate in West Antarctica, from Sixteen Ice Cores

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    Sixteen high-resolution ice-core records from West Antarctica and South Pole are used to examine the spatial and temporal distribution of sulfate for the last 200 years. The preservation of seasonal layers throughout the length of each record results in a dating accuracy of better than 1 year based on known global-scale volcanic events. A dual transport source for West Antarctic sea-salt (ss) SO42- and excess (xs) SO42- is observed: lower-tropospheric for areas below 1000m elevation and mid-/upper-tropospheric/stratospheric for areas located above 1000m. Our XsSO(4)(2-) records with volcanic peaks removed do not display any evidence of an anthropogenic impact on West Antarctic SO42- concentrations but do reveal that a major climate transition takes place over West Antarctica at similar to 1940. Global-scale volcanic eruptions appear as significant peaks in the robust-spline residual xsSO(4)(2-) records from sites located above 1000 m elevation but do not appear in the residual records from sites located below 1000 m

    Initial in vitro screening approach to investigate the potential health and environmental hazards of Enviroxℱ – a nanoparticulate cerium oxide diesel fuel additive

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    Nanotechnology is the new industrial revolution of the 21st Century as the various processes lead to radical improvements in medicine, manufacturing, energy production, land remediation, information technology and many other everyday products and applications. With this revolution however, there are undoubted concerns for health, safety and the environment which arise from the unique nature of materials and processes at the nanometre scale

    Progressing delineations of key biodiversity areas for seabirds, and their application to management of coastal seas

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    Aim: Decision-making products that support effective marine spatial planning are essential for guiding efforts that enable conservation of biodiversity facing increasing pressures. Key Biodiversity Areas (KBAs) are a product recently agreed upon by an international network of organizations for identifying globally important areas. Utilizing the KBA framework, and by developing a conservative protocol to identify sites, we identify globally importants places for breeding seabirds throughout the coastal seas of a national territory. We inform marine spatial planning by evaluating potential activities that may impact species and how a proposed network of Marine Management Areas (MMAs) overlap with important sites. Location: Southwest Atlantic Ocean. Methods: We collated a national inventory of all breeding locations for seabirds, including abundance records where available, and complementary estimates of at-sea distribution. We delineated areas of importance in coastal seas following approaches tailored to the ecology of species and assessed areas against global KBA criteria. To determine opportunities for species conservation and management, we reviewed which human activities have been documented to impact the target species globally via IUCN Red List accounts, and also assessed the overlap of important sites with a proposed MMA network. Results: We identified global KBAs for nine seabird species (Anatidae, Diomedeidae, Laridae, Procellariidae, Spheniscidae, Stercorariidae) throughout national coastal seas. Globally important areas where multiple species overlapped were only partially accounted for in key zones of the proposed MMA network. Main Conclusions: Development of a conservative protocol to identify marine sites for assessment against KBA criteria, revealed opportunities for enhancing a network of proposed Marine Management Areas in coastal seas. The framework we apply in this study has broad relevance for other systems where the design or review of management plans for the marine environment is required.info:eu-repo/semantics/publishedVersio

    Detection of a new non-native freshwater species by DNA metabarcoding of environmental samples — first record of Gammarus fossarum in the UK

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    © 2017 The Author(s). and 2017 REABIC. We report the discovery of a non-native gammarid, Gammarus fossarum (Koch, 1836) (Crustacea, Amphipoda), in UK rivers. Gammarus fossarum is a common freshwater gammarid in many parts of mainland Europe, but was previously considered absent from the UK. Gammarus fossarum was detected in a number of UK rivers following DNA metabarcoding of a mini-barcode region of the COI gene in macroinvertebrate kick samples, and environmental DNA (eDNA) from water and sediment samples. Subsequent morphological analysis and standard DNA barcoding showed that the species could be reliably identified and separated from Gammarus pulex (Linnaeus, 1758), the most dominant and widespread native freshwater gammarid in the UK. Our data demonstrate extensive geographical coverage of G. fossarum in the UK, spanning distant river catchments. At present there is no data to confirm the likely origin of G. fossarum’s introduction. Subsequent re-examination of historic archive material shows the species to have been present in the UK since at least 1964. This study is among the first to demonstrate the potential of eDNA metabarcoding for detection of new non-native species
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