Functional analysis of sandstone ground stone tools: arguments for a qualitative and quantitative synergetic approach

In the last few years, the application of quantitative methods in the field of use wear analysis has grown considerably, involving the use of different techniques. A development in surface measurements approaches has become necessary as standard assessments based upon qualitative functional analysis are often affected by a degree of subjectivity and a limited reproducibility. To advance the current methodological debate on functional analysis of ground stone technology, we present a combined methodological approach, including qualitative and quantitative analyses, applied to the study of experimental sandstone ground stone tools. We test surface quantification at a macro and micro-scale, paired with the observation and description of residue and use wear connected to the processing of plant, animal and mineral matters. Our results provide an exhaustive quantitative dataset concerning surface modifications associated with different uses and suggest an analytical workflow for the functional analysis of both experimental and archaeological ground stone assemblages. We also highlight the limitation and pitfalls of an exclusive adoption of quantitative methods in the study of ancient tool use demonstrating how a synergetic approach can enhance the quality, reproducibility and comparability of functional data

Early evidence of stone tool use in bone working activities at Qesem Cave, Israel

For a long while, the controversy surrounding several bone tools coming from pre-Upper Palaeolithic contexts favoured the view of Homo sapiens as the only species of the genus Homo capable of modifying animal bones into specialised tools. However, evidence such as South African Early Stone Age modified bones, European Lower Palaeolithic flaked bone tools, along with Middle and Late Pleistocene bone retouchers, led to a re-evaluation of the conception of Homo sapiens as the exclusive manufacturer of specialised bone tools. The evidence presented herein include use wear and bone residues identified on two flint scrapers as well as a sawing mark on a fallow deer tibia, not associated with butchering activities. Dated to more than 300 kya, the evidence here presented is among the earliest related to tool-assisted bone working intended for non-dietary purposes, and contributes to the debate over the recognition of bone working as a much older behaviour than previously thought. The results of this study come from the application of a combined methodological approach, comprising use wear analysis, residue analysis, and taphonomy. This approach allowed for the retrieval of both direct and indirect evidence of tool-assisted bone working, at the Lower Palaeolithic site of Qesem Cave (Israel)

The application of 3D modeling and spatial analysis in the study of groundstones used in wild plants processing

In recent years, several works have proved the reliability of the application of 3D modeling and spatial analysis in the study of stone tool use. Monitoring surface morphometry resulting from the use of lithic tools has the potential to objectively quantify and identify patterns of modifications associated to specific activities and worked materials. In particular, the combination of surface morphometry with a systematic experimental framework and use wear analysis has the potential of foreseeing residue distribution areas over the groundstone surfaces, hence providing a key aid in establishing sampling strategies applied to archeological specimens. Here, we propose an approach that applies 3D modeling, performed through a close-range photogrammetry, and the use of GIS software to investigate surface modifications and residue distribution on groundstones used to process wild plants. Our work comprises a dedicated experimental framework in which modern tool replicas have been used to process different species of wild plant foods through grinding, crushing, and pounding. By applying 3D modeling and spatial analysis, we were able to characterize patterns of surface modifications related to each of the worked substances and activities performed. Moreover, we monitored the distribution of starch granules over the experimental groundstone surfaces and its variation in relation to the state of the worked substance and the action carried out. Our results provide one of the first experimental dataset focused on the use of groundstones for wild plant processing, and a reliable methodology for further studies related to the exploitation of stone technology and wild vegetal substances in the past

The arrival of millets to the Atlantic coast of northern Iberia

Despite being one of the most important crops in the recent prehistory of Eurasia, the arrival and exploitation of millets in the westernmost part of Europe are still largely underexplored. Here and for the first time, we report multipronged biomolecular evidence of millet consumption along the Atlantic façade of northern Iberia through a combination of radiocarbon dating, stable isotopes, and dental calculus analyses on the human individuals found in the burial site of El Espinoso cave (Asturias, Spain). The high-resolution chronological framework established for individuals placed the burials between 1235 and 1099 cal. BC. The discovery of high ?13C values on their bone collagen and the identification of polyhedral starch grains within their dental plaque underline the relevance of C4 plants in their diet and highlights the timing of the systematic consumption of millets in the Late Bronze Age. Our data support previous regional archaeobotanical evidence and establish a more precise chronology of the dispersal of millets into northern Iberia during the Bronze Age, becoming an essential crop until the arrival of maize from America after AD 1492. This study emphasizes the importance of multidisciplinary methods to ascertain the origin and development of agricultural practices during recent prehistory.This study is part of B.G.R.’s Doctoral dissertation, supervised by A.B.M.A. and M.R.G.M. This research is funded by the research projects of the Spanish Economy, Industry and Competitiveness Ministry HAR2016-75605-R to M.R.G.M. and HAR2017- 84997-P to A.B.M.A. Part of this research, including the stable isotope analyses, was funded by the ERC Consolidator Grant (SUBSILIENCE ref. 818299), awarded to A.B.M.A. Analysis of dental calculus was carried out in the framework of the ERC Starting Grant (HIDDEN FOODS ref. 639286), awarded to E.C. The authors would like to thank C. García de Castro and B. López for giving information and allowing the sampling of Los Cinchos cave. We are grateful to L. Agudo Perez for her invaluable help during the collagen extraction protocols. The authors wish to acknowledge Dr Lawrence G. Straus (University of New Mexico) for editing the English version of this manuscript. Finally, we also thanks to two anonymous reviewers for their constructive comments that improved the manuscript

Different Resorptive Patterns of Two Avulsed and Replanted Upper Central Incisors Based on Scanning Electron Microscopy and Stereomicroscopic Analysis: A Case Report

Dental trauma resulting in permanent tooth avulsion commonly affects the young population. The prognosis of replantation after avulsion depends on the natural history of inflammatory and replacement resorption. Several risk factors for type and onset of external resorption have been defined. This case study describes different resorptive patterns observed in two upper central incisors belonging to a single individual, avulsed in the same moment, and replanted after thirty-six hours of dry storage. The roots were analyzed by scanning electron microscopy and stereomicroscope imaging, to obtain an in-depth analysis of the resorptive pattern. The aim of this report is to: (i) underline the high variability in the incidence of root resorption after replantation across and within types of teeth and resorption; and (ii) underline the possible concurrence of different factors affecting the onset and type of resorptive pattern. In conclusion, an unpredictable pattern of resorption may account for the poor prognosis when teeth are replanted outside the current recommendations

Wild cereal grain consumption among Early Holocene foragers of the Balkans predates the arrival of agriculture

Forager focus on wild cereal plants has been documented in the core zone of domestication in southwestern Asia, while evidence for forager use of wild grass grains remains sporadic elsewhere. In this paper, we present starch grain and phytolith analyses of dental calculus from 60 Mesolithic and Early Neolithic individuals from five sites in the Danube Gorges of the central Balkans. This zone was inhabited by likely complex Holocene foragers for several millennia before the appearance of the first farmers similar to 6200 cal BC. We also analyzed forager ground stone tools (GSTs) for evidence of plant processing. Our results based on the study of dental calculus show that certain species of Poaceae (species of the genus Aegi(ops) were used since the Early Mesolithic, while GSTs exhibit traces of a developed grass grain processing technology. The adoption of domesticated plants in this region after similar to 6500 cal BC might have been eased by the existing familiarity with wild cereals

Multipronged dental analyses reveal dietary differences in last foragers and first farmers at Grotta Continenza, central Italy (15,500–7000 BP)

This paper provides results from a suite of analyses made on human dental material from the Late Palaeolithic to Neolithic strata of the cave site of Grotta Continenza situated in the Fucino Basin of the Abruzzo region of central Italy. The available human remains from this site provide a unique possibility to study ways in which forager versus farmer lifeways affected human odonto-skeletal remains. The main aim of our study is to understand palaeodietary patterns and their changes over time as reflected in teeth. These analyses involve a review of metrics and oral pathologies, micro-fossils preserved in the mineralized dental plaque, macrowear, and buccal microwear. Our results suggest that these complementary approaches support the assumption about a critical change in dental conditions and status with the introduction of Neolithic foodstuff and habits. However, we warn that different methodologies applied here provide data at different scales of resolution for detecting such changes and a multipronged approach to the study of dental collections is needed for a more comprehensive and nuanced understanding of diachronic changes

New evidence for the Middle to Upper Palaeolithic transition interval in the Danube Gorges of the Balkans

Over the past few years, new investigations in northern Bulgaria at the site of Bacho Kiro have revived the likelihood that the "Danube corridor" route served as a primary axis for the dispersal of modern humans into Europe. The association of modern humans with material remains of Protoaurignacian and Aurignacian provenance and/or transitional industries, would push the start of the dispersal across this region to 47 ka. Furthermore, fossil remains from the cave site of Peștera cu Oase in the Romanian hinterland of the Danube Gorges area of the north-central Balkans provide genetic evidence of admixtures between Neanderthal and modern human populations that might have taken place precisely along this transitory corridor. Yet, there is still relatively little in the way of evidence about, on the one hand, the last Middle Palaeolithic, and by proxy Neanderthal, and, on the other hand, the Initial and Early Upper Palaeolithic, and by proxy modern human, settlement of the region. Our recent investigations in the Danube Gorges area have brought to light two new sites, Tabula Traiana Cave and Dubočka-Kozja Cave, with Middle to Upper Palaeolithic deposits. The application of modern standards of recovery and recording have enabled us to apply a suite of cutting edge and state-of-the-art methodologies backed by extensive radiometric dating of these sites’ deposits. In this paper, we will present most recently obtained radiocarbon accelerator mass spectrometry (AMS) measurements, which allow us to discuss the chronological attribution of different levels of the two sites with more certainty. We will also offer further details regarding the knapped stone assemblages, including the results of use-wear analyses on a select number of artefacts. Finally, this evidence is integrated with the results obtained through the analyses of the faunal assemblages and by characterizing taphonomic factors that impacted their formation. Complementary data come from a relatively large pool of unidentifiable bone samples analyzed through the application of proteomic fingerprinting known as the Zooarchaeology by Mass Spectrometry (ZooMS), which has allowed us to better characterize the animal taxa composition of the faunal assemblages from the two sites and identify agents of bone accumulation. The results indicate a late continuation of the Middle Palaeolithic presence characterized by a Levallois-derived lithic industry at one of the two sites and the broadly contemporaneous appearance of the Early Upper Palaeolithic tools in the lithic assemblage of the other site. We discuss how the locations of the two sites in this specific landscape zone along the Danube might have influenced their respective uses