Trust Building Tools for Talking with Youth During Times of Conflict

This presentation will provide tools and strategies for turning crisis into opportunity. The skills of Life Space Crisis Intervention provide helping adults faced with the extreme behavior of youth during times of crisis strategies which build trust and connections in meaningful and healing ways; turning crisis into opportunity for at risk youth to develop social responsibility and grow emotionall

Fluoreszenzsonden zur Quantifizierung des chelastisierbaren Eisenpools in Kompartimenten vitaler Zellen

Eisen in der Zelle besitzt im wesentliche eine essentielle Bedeutung. So ist es ein wesentlicher Bestandteil vieler wichtiger Funktionsproteine. Es kann in seiner freien und ungebundenen Form aber auch toxisch wirken. In seiner “freien Form“, lediglich schwach gebunden an niedermolekulare Liganden ist es in der Lage Reaktionen einzugehen, bei denen hoch reaktive Sauerstoffspezies entstehen können. Dieser „chelatisierbare Eisenpool“ wird in entscheidenem Maße für unterschiedliche Zellschädigungsprozesse verantwortlich gemacht. Mit Hilfe von Fluoreszenzsonden kann der chelatisierbare Eisenpool in verschiedenen Kompartimenten der Zelle charakterisiert und quantifiziert werden. Die Fluoreszenz, der in die Zelle eingebrachten Eisen-chelatisierenden Indikatoren, wird durch vorhandenes chelatisierbares Eisen aufgehoben. Durch Zugabe eines membrangängigen Eisenchelators im Überschuss wird diese Löschung wieder aufgehoben, da der Chelator das Eisen vom Indikator entfernt. Als Folge der Zugabe kann ein Anstieg der Fluoreszenzintensität beobachtet werden. Die Zunahme der Intensität ist ein Maß für die Menge an freiem Eisen und kann quantifiziert werden. Durch Anbindung einer Zielsteuerungskomponente an die Sonde soll eine organellspezifische Akkumulation erfolgen. Es konnten Sonden zur Charakterisierung des chelatisierbaren Eisenpools in den Mitochondrien und den Lysosomen / Endosomen entwickelt werden

Pertimbangan Hakim Dalam Menjatuhkan Putusan Pidana Terhadap Pelaku Tindak Pidana Pembunuhan Pada Sengketa Tanah Di Desa Bedalewun, Kecamatan Ile Boleng, Kabupaten Flores Timur (Studi Putusan No. 100/Pid.B/2013/Pn.Ltk)

The purpose of this legal research is to know and analyze the judge's consideration in imposing criminal judgment on the perpetrators of murder in land dispute in Bedalewun village, Ile Boleng subdistrict, East Flores regency (Study of Decision Number.100/PID.B /2013/PN. LTK). The research method was normative legal research, which is to describe written legal norm about judge's consideration in giving sentence according to juridical and non-juridical consideration. The study was conducted by interviewing the judges in the Sleman District Court. The result of this research indicated that the before giving sentence in this case Number 100/PID.B/2013/PN. LTK., the judges considered both juridical and non juridical consideration. The juridical consideration are: prosecutor's indictment, prosecutor's demand, and proof and evidence presented by the public prosecutor. While nonjuridical consideration are matters relating to the duty of judges to explore, follow, and understand the values of law and sense of justice that are available in the life of Lamaholot society

Immunization Trials with Recombinant Major Sperm Protein of the Bovine Lungworm Dictyocaulus viviparus

The lungworm Dictyocaulus viviparus is one of the most economically important bovine parasites in temperate climate regions. Following infection, D. viviparus induces a temporary protective immunity, and a vaccine based on attenuated, infective larvae is commercially available. However, due to several disadvantages of the live vaccine, the development of a recombinant subunit vaccine is highly desirable. Therefore, the major sperm protein (MSP), which is essential for the parasite’s reproduction, was tested as a recombinantly Escherichia coli-expressed glutathione-S-transferase (GST)-fused vaccine antigen in immunization trials with two different adjuvants, Quil A and Al(OH)3. Calves (N = 4 per group) were immunized on study day (SD) 0, 21 and 42 and given a challenge infection on SD 63–65. The two control groups received only the respective adjuvant. Based on geometric means (GM), a 53.64% reduction in larvae per female worm was observed in the rMSP Quil A group vs. its control group (arithmetic means (AM): 54.43%), but this difference was not statistically significant. In the rMSP Al(OH)3 group, the mean number of larvae per female worm was even higher than in the respective control group (GM: 9.24%, AM: 14.14%). Furthermore, male and female worm burdens and the absolute number of larvae did not differ significantly, while the Al(OH)3 control group harbored significantly longer worms than the vaccinated group. Vaccinated animals showed a rise in rMSP-specific antibodies, particularly IgG and its subclass IgG1, and the native protein was detected by immunoblots. Although rMSP alone did not lead to significantly reduced worm fecundity, it might still prove useful as part of a multi-component vaccine

Random forest classification as a tool in epidemiological modelling: Identification of farm-specific characteristics relevant for the occurrence of Fasciola hepatica on German dairy farms

Fasciola hepatica is an internal parasite of both human and veterinary relevance. In order to control fasciolosis, a multitude of attempts to predict the risk of infection such as risk maps or forecasting models have been developed. These attempts mainly focused on the influence of geo-climatic and meteorological features. Predicting bovine fasciolosis on farm level taking into account farm-specific settings yet remains challenging. In the present study, a new methodology for this purpose, a data-driven machine learning approach using a random forest classification algorithm was applied to a cross-sectional data set of farm characteristics, management regimes, and farmer aspects within two structurally different dairying regions in Germany in order to identify factors relevant for the occurrence of F. hepatica that could predict farm-level bulk tank milk positivity. The resulting models identified farm-specific key aspects in regard to the presence of F. hepatica. In study region North, farm-level production parameters (farm-level milk yield, farm-level milk fat, farm-level milk protein), leg hygiene, body condition (prevalence of overconditioned and underconditioned cows, respectively) and pasture access were identified as features relevant in regard to farm-level F. hepatica positivity. In study region South, pasture access together with farm-level lameness prevalence, farm-level prevalence of hock lesions, herd size, parity, and farm-level milk fat appeared to be important covariates. The stratification of the analysis by study region allows for the extrapolation of the results to similar settings of dairy husbandry. The local, region-specific modelling of F. hepatica presence in this work contributes to the understanding of on-farm aspects of F. hepatica appearance. The applied technique represents a novel approach in this context to model epidemiological data on fasciolosis which allows for the identification of farms at risk and together with additional findings in regard to the epidemiology of fasciolosis, can facilitate risk assessment and deepen our understanding of on-farm drivers of the occurrence of F. hepatica