A COMPARISON OF THE BILATERAL, DYNAMIC Q-ANGLE IN FEMALES

The purpose of this study was to investigate the Q-angle during the stance phase of walking and jogging. Twenty-one females were recruited to participate in the study. Subjects were filmed walking and jogging on the treadmill and the bilateral, Q-angle measurements were calculated for heel-strike (HS), mid-stance (MS), and toe-off (TO) at each speed. Significant differences (p < 0.05) in the Q-angle were found between HS and TO and between MS and TO for both legs during walking. There were no significant differences found during jogging for the left leg; however, there were significant differences in the right leg from MS to TO. A comparison of the Q-angle values showed that HS, MS, and TO were significantly different between the right and left legs for walking, and only MS and TO were significantly different for jogging

POLTERGEIST Encodes a Protein Phosphatase 2C that Regulates CLAVATA Pathways Controlling Stem Cell Identity at Arabidopsis Shoot and Flower Meristems

AbstractBackground: Receptor kinases are a large gene family in plants and have more than 600 members in Arabidopsis. Receptor kinases in plants regulate a broad range of developmental processes, including steroid hormone perception, organ elongation, self-incompatibility, and abscission. Intracellular signaling components for receptor kinases in plants are largely unknown. The CLAVATA 1 (CLV1) receptor kinase in Arabidopsis regulates stem cell identity and differentiation through its repression of WUSCHEL (WUS) expression. Mutations at the POLTERGEIST (POL) gene were previously described as phenotypic suppressors of mutations within the CLV1 gene. Genetic evidence placed POL as a downstream regulator of CLAVATA1 signaling.Results: We provide evidence that POL functions in both the CLV1-WUS pathway and a novel WUS-independent CLV1 pathway regulating stem cell identity. We demonstrate that POL encodes a protein phosphatase 2C (PP2C) with a predicted nuclear localization sequence, indicating that it has a role in signal transduction downstream of the CLV1 receptor. The N terminus of POL has a possible regulatory function, and the C terminus has PP2C-like phosphatase catalytic activity. Although the POL catalytic domain is conserved in other PP2Cs, the POL protein represents a unique subclass of plant PP2Cs. POL is broadly expressed throughout the plant.Conclusions: POL represents a novel component of the CLV1 receptor kinase signaling pathway. The ubiquitous expression of POL and pol phenotypes outside the meristem suggest that POL may be a common regulator of many signaling pathways

Effects of a Mindfulness Intervention to Improve Teachers’ Well-being

Teacher attrition has increased by 50% over the past 15 years. The purpose of this study was to determine whether a mindfulness-based intervention would be effective for reducing stress and increasing mindfulness, self-efficacy, job satisfaction, and well-being among teachers at Title I (low-income) schools. The researchers evaluated the effects of a mindfulness intervention created specifically for teachers using a four-way ANOVA (2 × 3 × 2 × 5) with two between subjects’ factors, groups and levels, and two within subjects/repeated measures factors, pretest-posttest and scales. While the study did not show significant effects for mindfulness, stress, self-efficacy, and well-being, teachers in the control group demonstrated significantly lower levels of job satisfaction compared to the experimental group. These results are commensurate with previous studies that suggest with a mindfulness-based intervention, teachers report increased job satisfaction

On the detectability of star-planet interaction

Magnetic (or tidal) interactions between "hot Jupiters" and their host stars can potentially enhance chromospheric and coronal activity. An ideal testbed for investigating this effect is provided by the extreme WASP-18 system, which features a massive (~10 times Jupiter) close-in (~1 day period) transiting planet orbiting a young F6 star. Optical and X-ray observations of WASP-18 were conducted in November 2011. The high-resolution echelle spectrograph MIKE was used on the 6.5m Magellan Clay telescope to obtain 13 spectra spanning planetary orbital phases of 0.7-1.4, while the X-ray Telescope on Swift provided contemporaneous monitoring with a stacked exposure of ~50 ks. The cores of the Ca II H and K lines do not show significant variability over multiple orbits spanning ~8 d, in contrast to the expectation of phase-dependent chromospheric activity enhancements for efficient star-planet interaction. The star is also X-ray faint, with log Lx < 27.6 erg/s (0.3-2 keV), indicating that coronal activity is likewise low. The lack of detectable star-planet interaction in this extreme system requires that any such effect must here be transient, if indeed present. We demonstrate that searches for Ca II H and K variability can potentially mistake a stellar hotspot, if observed over a short segment of the rotation period, for planet-induced activity. Taken together, these results suggest that the utility of star-planet interaction as a robust method of estimating exoplanet magnetic field strengths may be limited.Comment: Accepted to ApJ; 9 pages emulateapj, 5 figures, 1 table (v2: corrected fn15, typos, refs

Household water insecurity experiences and their perceived determinants in a low-income community of Cartagena, Colombia, during a water service expansion project

Traditional water indicators primarily focus on water quantity and quality, but emergent research demonstrates that measurement of lived experiences with water availability, accessibility, and use is important for understanding how household water insecurity impacts health and well-being. Few empirical studies have explored which household water insecurity experiences are most salient, or their potential causes, in Latin American cities. We analyzed data from 266 households in a low-income settlement of Cartagena, Colombia, to identify correlates and perceived determinants of water insecurity. The most prevalent household water insecurity experiences were water supply interruptions (96%), water worry (94%), and anger about the water situation (90%). Unexpected water interruptions and use of non-piped primary drinking water sources were associated with greater household water insecurity scores, water worry subscores, and hygiene subscores. Respondents perceived water issues in their community to be caused by deficiencies in gray infrastructure (49%), which included deficiencies in water distribution, treatment, or storage technologies. Social infrastructure (36%), including issues with political, economic, or administrative systems, was also cited as a barrier to water security. We did not detect significant relationships between water insecurity scores and the attribution of these problems to gray or social infrastructure, but there may be relationships between these factors and duration of residency and using a non-piped water source. These findings underscore the importance of socio-political factors and community engagement for improving urban water insecurity through slum-upgrade projects

Hidden costs: The ethics of cost-effectiveness analyses for health interventions in resource-limited settings

Cost-effectiveness analysis (CEA) is an increasingly appealing tool for evaluating and comparing health-related interventions in resource-limited settings. The goal is to inform decision-makers regarding the health benefits and associated costs of alternative interventions, helping guide allocation of limited resources by prioritizing interventions that offer the most health for the least money. Although only one component of a more complex decision-making process, CEAs influence the distribution of healthcare resources, directly influencing morbidity and mortality for the world’s most vulnerable populations. However, CEA-associated measures are frequently setting-specific valuations, and CEA outcomes may violate ethical principles of equity and distributive justice. We examine the assumptions and analytical tools used in CEAs that may conflict with societal values. We then evaluate contextual features unique to resource-limited settings, including the source of health-state utilities and disability weights; implications of CEA thresholds in light of economic uncertainty; and the role of external donors. Finally, we explore opportunities to help align interpretation of CEA outcomes with values and budgetary constraints in resource-limited settings. The ethical implications of CEAs in resource-limited settings are vast. It is imperative that CEA outcome summary measures and implementation thresholds adequately reflect societal values and ethical priorities in resource-limited settings

Echinococcus across the north : Current knowledge, future challenges

Foodborne Parasites in Cold ClimatesAbstract Zoonotic Echinococcus spp. cestodes are present in almost all circumpolar nations, and have historically posed a risk to health of indigenous as well as other northern residents. However, surveillance data on both alveolar (AE) and cystic (CE) echinococcosis remains incomplete throughout the circumpolar region: Russia, Fennoscandia, Iceland, Greenland, Canada and Alaska (USA). Prevalence of Echinococcus spp. varies considerably in definitive canid hosts, animal intermediate hosts and accidental hosts like humans. Yet despite the high prevalence reported in canids in some geographic locations, human AE and CE are much less common than in endemic Asian and central European countries. This paper explores knowledge gaps and future challenges posed by Echinococcus spp. in eight circumpolar countries, a region where rapid environmental and social change are rewriting the boundaries, transmission, and impact of many pathogens, including zoonotic Echinococcus spp. Genotypes G6, G8 and G10 of Echinococcus canadensis are causative agents of human CE and have been identified in sylvatic (wild animal) and synanthropic (ecological association with humans) cervid-canine life cycles in the following northern regions: Alaska and northern Canada - G8 and G10; northern Russia - G6, G8, G10; and Fennoscandia - G10 in Finland - with no recent reports from Norway or Sweden. Echinococcus multilocularis, which causes AE, has been identified in a sylvatic arvicoline rodent-canine lifecycle in Alaska, Canada, Russia, Sweden and Svalbard (Norway). Asian, Mongolian, European and North American strains of E. multilocularis are found in Russia, with the North American N1 strain predominating in the north. The N1 strain is also found in Alaska, as well as Svalbard, whilst Asian strains have been identified in western Alaska. Central North American (N2) strain and European-type strains of E. multilocularis are present in Canada. Typing of the strain in Sweden is still pending. Individual human cases of AE with N2 and European-type strains are reported in North America, as well as multiple cases with Asian strains in Russia and historically on St Lawrence Island, Alaska (although genotyping of human cases was not available at the time). Echinococcus spp. have not been detected in Greenland and have been eliminated from Iceland. The predominance of E. multilocularis N1 strain and E. canadensis genotypes, in regions with high prevalence in definitive hosts yet low incidence of human AE and CE, suggests that these genotypes have lower zoonotic potential and pathogenicity than European and Asian strains of E. multilocularis and livestock genotypes of E granulosus sensu stricto. The continued monitoring of the emergence of Echinococcus genotypes within definitive and intermediate hosts, as well as people, is needed to assess the impact on public health risk, since the introduction of other genotypes could have serious repercussions. Lastly, determining risk factors and source attribution for human cases, including the possibility of food and waterborne transmission and the likelihood of autochthonous transmission, remain challenges.Peer reviewe

Understanding flavin electronic structure and spectra

Flavins have emerged as central to electron bifurcation, signaling, and countless enzymatic reactions. In bifurcation, two electrons acquired as a pair are separated in coupled transfers wherein the energy of both is concentrated on one of the two. This enables organisms to drive demanding reactions based on abundant low-grade chemical fuel. To enable incorporation of this and other flavin capabilities into designed materials and devices, it is essential to understand fundamental principles of flavin electronic structure that make flavins so reactive and tunable by interactions with protein. Emerging computational tools can now replicate spectra of flavins and are gaining capacity to explain reactivity at atomistic resolution, based on electronic structures. Such fundamental understanding can moreover be transferrable to other chemical systems. A variety of computational innovations have been critical in reproducing experimental properties of flavins including their electronic spectra, vibrational signatures, and nuclear magnetic resonance (NMR) chemical shifts. A computational toolbox for understanding flavin reactivity moreover must be able to treat all five oxidation and protonation states, in addition to excited states that participate in flavoprotein's light-driven reactions. Therefore, we compare emerging hybrid strategies and their successes in replicating effects of hydrogen bonding, the surrounding dielectric, and local electrostatics. These contribute to the protein's ability to modulate flavin reactivity, so we conclude with a survey of methods for incorporating the effects of the protein residues explicitly, as well as local dynamics. Computation is poised to elucidate the factors that affect a bound flavin's ability to mediate stunningly diverse reactions, and make life possible.DFG, 390540038, EXC 2008: Unifying Systems in Catalysis "UniSysCat

Providing modeling tools on extreme events of climate change to Puget Sound managers

As climate change becomes a reality for the management of Puget Sound, water resource and fisheries managers should consider incorporating predictions and outcomes of future climate drivers into their long-range plans and daily operations. Modeling tools that focus on climate impacts and predictions show that extreme events are more often responsible for large impacts than the long-term press of climate change. Working with water resource and fisheries managers in the Dungeness and Skagit watersheds, this project uses outputs of existing climate and estuarine models to define thresholds and metrics associated with extreme climate-driven events that are of importance to the resource managers. Managers from the Dungeness and Skagit basins were brought together to assist with defining information needs for sustainable fish habitat and human water uses. The resource managers participating in the project include municipal waste water treatment operators and planners, fisheries managers, agricultural practitioners and conservation district staff, flood control specialists, and others. The information needs identified by the planners, based on the climate model outputs, include better predictions for low stream flows, stream temperature, extent of salinity intrusion into tidal rivers, and timing of extreme events that fall outside the historical norm. The project is developing a decision-support system to meet these needs. The metrics used to drive the decision-support system are derived from model outputs, driven by resource management needs. The information needs, metrics derived from existing models, and the draft decision-support system will be presented. The research team also seeks to use the project to define improved communication pathways between the scientific community and local managers

Comparison of conventional and Alltech beef PN finishing programs: performance and

The objective of this study was to compare the feedlot and carcass performance of the PN Beef Program in relation to a conventional feedlot diet when both diets are combined with or without exogenous growth promotants