Pure shift amide detection in conventional and TROSY-type experiments of 13^{13}C,15^{15}N-labeled proteins

Large coupling networks in uniformly $^{13}$C,$^{15}$N-labeled biomolecules induce broad multiplets that even in flexible proteins are frequently not recognized as such. The reason is that given multiplets typically consist of a large number of individual resonances that result in a single broad line, in which individual components are no longer resolved. We here introduce a real-time pure shift acquisition scheme for the detection of amide protons which is based on 13C-BIRDr,X. As a result the full homo- and heteronuclear coupling network can be suppressed at low power leading to real singlets at substantially improved resolution and uncompromised sensitivity. The method is tested on a small globular and an intrinsically disordered protein (IDP) where the average spectral resolution is increased by a factor of ~ 2 and higher. Equally important, the approach works without saturation of water magnetization for solvent suppression and exchanging amide protons are not affected by saturation transfer

Visualising the Imageless Past: Film and Audio-Visual Media in Archaeological Museums

This paper focuses on the use of film and audio-visual media in an exhibition context, as a means  for mediating the objects of culture on display, which in turn supports the narration of the exhibition. The paper focuses on two German museums, one of archaeology and the other of prehistory, to examine exhibition practices through the different functions of audio-visual media. How does audio-visual media help to visualise the past? How do they contribute to unveil the immaterial side, i.e. the “meaning” and significance of the objects on display? What kind of picture does the synergy between material objects and audio-visual media paint of our ancestors? Moreover, how can audio-visual media help museums to deal with the basic problem of archaeology (and historiography in general) and the representation of history? Finally, in what ways are audio-visual media used to inform the discipline and methods of archaeology, as a process for gaining knowledge?The analysis will demonstrate how the exhibition spaces of prehistory museums have distinct ways of integrating moving images, thus reflecting the different attitudes they have towards the materiality and mediality of the museum object and, as a consequence, the representation of history in the museum space

Should neurologists diagnose and manage functional neurologic disorders? It is complicated

Whereas only neurologists can "rule in" functional neurologic disorders (FNDs)-using physical signs and semiologic features-their role in follow-up care remains debated. We outlined the arguments for and against a neurologist's primary role in both assessing and managing FNDs. Favorable arguments include the following: (1) FND presents neurologically, and thus, only neurologists can ascertain the etiology of new neurologic deficits appearing on follow-up, and (2) neurologic encounters facilitate acceptance of diagnosis and enhance treatment engagement. Counter arguments include the following: (1) FND is a Diagnostic and Statistical Manual of Mental Disorders, 5th Edition codified psychiatric disorder with largely psychiatric treatments, and (2) neurologists can reassess patients if new neurologic symptoms develop without playing a primary follow-up role. Although more research is needed to clarify optimal approaches, neurologic expertise could be leveraged for diagnostic and coordinating roles if the pool of neurologists, psychiatrists, psychotherapists, physical and occupational therapists, and other allied clinicians trained in the interdisciplinary care of FNDs is substantially increased

Using Technology to Increase Physical Activity in Health Profession Students

Health profession students may need help establishing and maintaining positive health behaviors when they are in college. This study explored the effectiveness of text messaging as an innovative method for promoting an increase in daily physical activity. A convenience sample (N = 134) was recruited from students at a college of Health and Human Services in Michigan. The participants were randomized into an intervention or control group (n = 67 each). The intervention group received daily affective text messages encouraging more physical activity by taking more steps. The control group received only messages reminding them to report their number of steps. All of the participants received a pedometer, completed a demographics and daily habits questionnaire, and completed the Perceived Stress Scale (PSS). There was no significant difference between the intervention and control groups in their number of daily steps. However, the most inactive participants had a significant increase in steps during the study period. Health profession students’ lifestyle behaviors have consequences, as they become caregivers in our dynamic, demanding health-care system. For those with the greatest need for physical activity, encouraging such activity via text messaging may improve their ability to care for themselves and their clients

Southwest Partnership Project Economic Development Team

Final project for URSP688L: Planning Technologies (Fall 2015). University of Maryland, College Park.The Southwest Partnership has made significant progress in exploring the existing status of commercial development in their seven neighborhoods. This project will support this work using geospatial analysis and visualization to further the Southwest Partnership’s economic development goals. Data provided by the Southwest Partnership on existing businesses was geocoded and represented on a map of the seven neighborhoods and their immediate surroundings. The businesses in the Southwest Partnership area were categorized according to a display-friendly schema. The three main layers presented are vacant parcels with commercial and industrial zoning, business locations by category, and transit lines and stops. Major observations include the residential nature of most northern and southern neighborhoods, with the most concentrated and varied business activity in the central Hollins Market neighborhood and its borders. This neighborhood is also well-served by transit, is commercially zoned, and contains multiple vacant properties, suggesting its suitability for future business development. The report concludes that geospatial portrayal of business locations and types can contribute to future economic development strategies in the Southwest Partnership. However, several further steps would help solidify the information base. More accurate and comprehensive business information with respect to operating status and confirmed type of business conducted at each location is necessary, likely by visual survey. Additionally, resident feedback about new businesses and locations will help validate the analysis presented here that summarized existing types, gaps, and possible locations for future enterprises. The user-friendly, online GIS format of the final map product should prove useful in both presenting the analysis to the Southwest Partnership and form a basis for future work.The Southwest Partnership (SWP), Baltimor

Note : a simple laser shutter with protective shielding for beam powers up to 1 W

We present the design of an inexpensive and reliable mechanical laser shutter and its electronic driver. A camera diaphragm shutter unit with several sets of blades is utilized to provide fast blocking of laser light and protective shielding of the shutter mechanism up to a laser beam power of 1 W. The driver unit is based on an Arduino microcontroller with a motor-shield. Our objective was to strongly reduce construction effort and expenditure by limiting ourselves to a small number of modular parts, which are readily available. We measured opening and closing durations of less than 800 µs, and a timing jitter of less than 25 µs for the fastest set of blades. No degradation of the shutter performance was observed over 5·10^4 cycles

From exercise intolerance to functional improvement: The second wind phenomenon in the identification of McArdle disease

McArdle disease is the most common of the glycogen storage diseases. Onset of symptoms is usually in childhood with muscle pain and restricted exercise capacity. Signs and symptoms are often ignored in children or put down to 'growing pains' and thus diagnosis is often delayed. Misdiagnosis is not uncommon because several other conditions such as muscular dystrophy and muscle channelopathies can manifest with similar symptoms. A simple exercise test performed in the clinic can however help to identify patients by revealing the second wind phenomenon which is pathognomonic of the condition. Here a patient is reported illustrating the value of using a simple 12 minute walk test.RSS is funded by Ciências sem Fronteiras/CAPES Foundation. The authors would like to thank the Association for Glycogen Storage Disease (UK), the EUROMAC Registry funded by the European Union, the Muscular Dystrophy Campaign, the NHS National Specialist Commissioning Group and the Myositis Support Group for funding

Circular RNAs in urine of kidney transplant patients with acute T Cell-mediated allograft rejection

BACKGROUND: Circular RNAs (circRNAs) have recently been described as novel noncoding regulators of gene expression. They are detectable in the blood of patients with acute kidney injury. We tested whether circRNAs were present in urine and could serve as new predictors of outcome in renal transplant patients with acute rejection. METHODS: A global circRNA expression analysis using RNA from urine of patients with acute T cell-mediated renal allograft rejection and control transplant patients was performed. Dysregulated circRNAs were confirmed in a cohort of 62 patients with acute rejection, 10 patients after successful antirejection therapy, 18 control transplant patients without rejection, and 13 stable transplant patients with urinary tract infection. RESULTS: A global screen revealed several circRNAs to be altered in urine of patients with acute rejection. Concentrations of 2 circRNAs including hsa_circ_0001334 and hsa_circ_0071475 were significantly increased. These were validated in the whole cohort of patients. hsa_circ_0001334 was upregulated in patients with acute rejection compared with controls. Concentrations of hsa_circ_0001334 normalized in patients with acute rejection following successful antirejection therapy. hsa_circ_0001334 was associated with higher decline in glomerular filtration rate 1 year after transplantation. CONCLUSIONS: CircRNA concentrations are significantly dysregulated in patients with acute rejection at subclinical time points. Urinary hsa_circ_0001334 is a novel biomarker of acute kidney rejection, identifying patients with acute rejection and predicting loss of kidney function

Isabelle/PIDE as Platform for Educational Tools

The Isabelle/PIDE platform addresses the question whether proof assistants of the LCF family are suitable as technological basis for educational tools. The traditionally strong logical foundations of systems like HOL, Coq, or Isabelle have so far been counter-balanced by somewhat inaccessible interaction via the TTY (or minor variations like the well-known Proof General / Emacs interface). Thus the fundamental question of math education tools with fully-formal background theories has often been answered negatively due to accidental weaknesses of existing proof engines. The idea of "PIDE" (which means "Prover IDE") is to integrate existing provers like Isabelle into a larger environment, that facilitates access by end-users and other tools. We use Scala to expose the proof engine in ML to the JVM world, where many user-interfaces, editor frameworks, and educational tools already exist. This shall ultimately lead to combined mathematical assistants, where the logical engine is in the background, without obstructing the view on applications of formal methods, formalized mathematics, and math education in particular.Comment: In Proceedings THedu'11, arXiv:1202.453

VirusBoxing: A HIIT-based VR boxing game

Physical activity or exercise can improve people's health and reduce their risk of developing several diseases; most importantly, regular activity can improve the quality of life. However, lack of time is one of the major barriers for people doing exercise. High-intensity interval training (HIIT) can reduce the time required for a healthy exercise regime but also bring similar benefits of regular exercise. We present a boxing-based VR exergame called VirusBoxing to promote physical activity for players. VirusBoxing provides players with a platform for HIIT and empowers them with additional abilities to jab a distant object without the need to aim at it precisely. In this paper, we discuss how we adapted the HIIT protocol and gameplay features to empower players in a VR exergame to give players an efficient, effective, and enjoyable exercise experience.Comment: Corresponding author: [email protected]