Preventing Billions from being Washed Offshore: a Growing Approach to Stopping International Drug Trafficking

Many of the approaches to stopping the growth of the drug problem have been based on two conflicting theories centered around supply and demand concepts. The United States, in supporting a supply-based approach, has aimed many of its drug programs toward the eradication of the narcotics trade, mainly in marijuana, cocaine and heroin, at the source, mostly Third World countries. The drug-producing countries, however, advocate a demand- oriented solution where the objective is to wipe out demand by rehabilitating drug users, thus eliminating the need for the supply. A third solution is now emerging, one that focuses on another aspect of trafficking that controls both supply and demand - money. Government officials are realizing that the lure of huge cash profits keeps the drug world well-populated and economically secure. Therefore, the development of law enforcement tools to attack the illegal profits of drug trafficking can be more effective than trying to regulate the source or the market for drugs. Seizing a drug operation\u27s profits and assets has proven to be more effective in shutting down the operation than simply putting the trafficker in jail

Botanicals as a zinc oxide alternative to protect intestinal cells from an Escherichia coli F4 infection in vitro by modulation of enterocyte inflammatory response and bacterial virulence

Pharmacological doses of zinc oxide (ZnO) have been widely used in pig industry to control post-weaning diarrhea (PWD) symptoms exacerbated by enterotoxigenic Escherichia coli F4 infections. Because of environmental issues and regulatory restrictions, ZnO is no longer sustainable, and novel nutritional alternatives to manage PWD are urgently required. Botanicals represent a wide class of compounds employed in animal nutrition because of their diverse beneficial functions. The aim of this study was to investigate the in vitro protective action of a panel of essential oils and natural extracts on intestinal Caco-2 cells against an E. coli F4 infection. Moreover, we explored the potential mechanisms of action of all the botanicals compared to ZnO. Amongst the others, thyme essential oil, grape seed extract, and Capsicum oleoresin were the most effective in maintaining epithelial integrity and reducing bacterial translocation. Their mechanism of action was related to the modulation of cellular inflammatory response, the protection of tight junctions' expression and function, and the control of bacterial virulence, thus resembling the positive functions of ZnO. Moreover, despite their mild effects on the host side, ginger and tea tree essential oils provided promising results in the control of pathogen adhesion when employed during the challenge. These outcomes support the advantages of employing selected botanicals to manage E. coli F4 infections in vitro, therefore offering novel environmentally-friendly alternatives to pharmacological doses of ZnO capable to modulate host-pathogen interaction at different levels during PWD in pigs

A Quali-quantitative evaluation approach to pedodiversity by multivariate analysis: introduction to the concept of "pedocharacter"

A model has been developed for the interpretation of the complexity of pedological systems; this is referred to as “pedocharacter”. The main aim of the model was to reduce the variables able to define soils and their relationships with the environment through the following quali-quantitative approach: i) definition of a fair number of qualitative characters; and ii) development of an analytic function, defined as “Land Relevance of the Factor”

Thymol as an Adjuvant to Restore Antibiotic Efficacy and Reduce Antimicrobial Resistance and Virulence Gene Expression in Enterotoxigenic Escherichia coli Strains

The continuous spread of antimicrobial resistance is endangering the efficient control of enterotoxigenic Escherichia coli (ETEC), which is mainly responsible for post-weaning diarrhea onset in piglets. Thymol, the key constituent of thyme essential oil, is already used in animal nutrition for its antimicrobial action. The aim of this study was to investigate the potential adjuvant effect of thymol to re-establish antibiotic efficacy against highly resistant ETEC field strains. Secondly, we evaluated the modulation of virulence and antibiotic resistance genes. Thymol showed the capacity to control ETEC growth and, when combined with ineffective antibiotics, it increased their antimicrobial power. In particular, it showed significant effects when blended with colistin and tetracycline, suggesting that the adjuvant effects rely on the presence of complementary mechanisms of action between molecules, or the absence of resistance mechanisms that inactivate antibiotics and target sites. Furthermore, our findings demonstrate that, when added to antibiotics, thymol can help to further downregulate several virulence and antibiotic resistance genes, offering new insights on the potential mechanisms of action. Therefore, in a one-health approach, our study supports the beneficial effects of combining thymol with antibiotics to restore their efficacy, together with the possibility of targeting gene expression as a pioneering approach to manage ETEC pathogenicity

A blend of selected botanicals maintains intestinal epithelial integrity and reduces susceptibility to Escherichia coli F4 infection by modulating acute and chronic inflammation in vitro

In the pig production cycle, the most delicate phase is weaning, a sudden and early change that requires a quick adaptation, at the cost of developing inflammation and oxidation, especially at the intestinal level. In this period, pathogens like enterotoxigenic Escherichia coli (ETEC) contribute to the establishment of diarrhea, with long-lasting detrimental effects. Botanicals and their single bioactive components represent sustainable well-recognized tools in animal nutrition thanks to their wide-ranging beneficial functions. The aim of this study was to investigate the in vitro mechanism of action of a blend of botanicals (BOT), composed of thymol, grapeseed extract, and capsicum oleoresin, in supporting intestinal cell health during inflammatory challenges and ETEC infections. To reach this, we performed inflammatory and ETEC challenges on Caco-2 cells treated with BOT, measuring epithelial integrity, cellular oxidative stress, bacterial translocation and adhesion, gene expression levels, and examining tight junction distribution. BOT protected enterocytes against acute inflammation: while the challenge reduced epithelial tightness by 40%, BOT significantly limited its drop to 30%, also allowing faster recovery rates. In the case of chronic inflammation, BOT systematically improved by an average of 25% the integrity of challenged cells (p < 0.05). Moreover, when cells were infected with ETEC, BOT maintained epithelial integrity at the same level as an effective antibiotic and significantly reduced bacterial translocation by 1 log average. The mode of action of BOT was strictly related to the modulation of the inflammatory response, protecting tight junctions’ expression and structure. In addition, BOT influenced ETEC adhesion to intestinal cells (−4%, p < 0.05), also thanks to the reduction of enterocytes’ susceptibility to pathogens. Finally, BOT effectively scavenged reactive oxygen species generated by inflammatory and H2O2 challenges, thus alleviating oxidative stress by 40% compared to challenge (p < 0.05). These results support the employment of BOT in piglets at weaning to help manage bacterial infections and relieve transient or prolonged stressful states thanks to the modulation of host-pathogen interaction and the fine-tuning activity on the inflammatory tone

Zipf and Heaps laws from dependency structures in component systems

Complex natural and technological systems can be considered, on a coarse-grained level, as assemblies of elementary components: for example, genomes as sets of genes, or texts as sets of words. On one hand, the joint occurrence of components emerges from architectural and specific constraints in such systems. On the other hand, general regularities may unify different systems, such as the broadly studied Zipf and Heaps laws, respectively concerning the distribution of component frequencies and their number as a function of system size. Dependency structures (i.e., directed networks encoding the dependency relations between the components in a system) were proposed recently as a possible organizing principles underlying some of the regularities observed. However, the consequences of this assumption were explored only in binary component systems, where solely the presence or absence of components is considered, and multiple copies of the same component are not allowed. Here, we consider a simple model that generates, from a given ensemble of dependency structures, a statistical ensemble of sets of components, allowing for components to appear with any multiplicity. Our model is a minimal extension that is memoryless, and therefore accessible to analytical calculations. A mean-field analytical approach (analogous to the "Zipfian ensemble" in the linguistics literature) captures the relevant laws describing the component statistics as we show by comparison with numerical computations. In particular, we recover a power-law Zipf rank plot, with a set of core components, and a Heaps law displaying three consecutive regimes (linear, sub-linear and saturating) that we characterize quantitatively

Conformational Studies by Dynamic NMR. 97.Structure, Conformation, Stereodynamics and Enantioseparation of Aryl Substituted Norbornanes.

CAN 140:163414 AN 2003:996217 CAPLUS (COPYRIGHT (C) 2008 ACS ON SCIFINDER (R)) ABSTRACT THE STRUCTURE OF A 1,7,7-TRIARYLNORBORNANE (I) HAS BEEN DETD. BY X-RAY DIFFRACTION AND WAS FOUND ESSENTIALLY EQUAL TO THAT PREDICTED BY MOL. MECHANICS CALCNS. RESTRICTED ROTATION OF THE ARYL GROUPS ALSO HAS BEEN OBSD. BY DYNAMIC NMR SPECTROSCOPY IN THIS COMPD. AND IN A NO. OF ANALOGOUSLY SUBSTITUTED NORBORNANES. THE ARYL-NORBORNANE BOND ROTATION BARRIERS WERE MEASURED BY LINE SHAPE ANAL. OF THE 13C NMR SPECTRA OBTAINED AT TEMPS. LOWER THAN -100 °C AND WERE FOUND TO COVER THE RANGE 6.0 TO 7.9 KCAL MOL-1. AN EXCEPTION WAS THE ROTATION INVOLVING THE O-ANISYL GROUP IN II, WHICH OCCURS NEAR AMBIENT TEMP. SINCE THE CORRESPONDING BARRIER IS MUCH HIGHER (14.4 KCAL MOL-1). IN ONE CASE (III) CONFIGURATIONAL ENANTIOMERS COULD BE SEPD. BY CHIRAL HPLC AND THE CORRESPONDING CD SPECTRA RECORDED