Extra-virgin olive oil phenols block cell cycle progression and modulate chemotherapeutic toxicity in bladder cancer cells

Epidemiological data indicate that the daily consumption of extra‑virgin olive oil (EVOO), a common dietary habit of the Mediterranean area, lowers the incidence of certain types of cancer, in particular bladder neoplasm. The aim of the present study was to evaluate the antiproliferative activity of polyphenols extracted from EVOO on bladder cancer (BCa), and to clarify the biological mechanisms that trigger cell death. Furthermore, we also evaluated the ability of low doses of extra‑virgin olive oil extract (EVOOE) to modulate the in vitro activity of paclitaxel or mitomycin, two antineoplastic drugs used in the management of different types of cancer. Our results showed that EVOOE significantly inhibited the proliferation and clonogenic ability of T24 and 5637 BCa cells in a dose‑dependent manner. Furthermore, cell cycle analysis after EVOOE treatment showed a marked growth arrest prior to mitosis in the G2/M phase for both cell lines, with the subsequent induction of apoptosis only in the T24 cells. Notably, simultaneous treatment of mitomycin C and EVOOE reduced the drug cytotoxicity due to inhibition of ROS production. Conversely, the co‑treatment of T24 cells with paclitaxel and the polyphenol extract strongly increased the apoptotic cell death at each tested concentration compared to paclitaxel alone. Our results support the epidemiological evidence indicating that olive oil consumption exerts health benefits and may represent a starting point for the development of new anticancer strategies

Olive oil phenols as potential source of chemopreventive and therapeutic agents against bladder cancer

The consumption of extra virgin olive oil (EVOO), a common dietary habit of the mediterranean people, seems to be related to a lower incidence of certain types of cancer including bladder neoplasm. Metastases are the major cause of bladder cancer-related deaths and targeting cell motility has been proposed as a therapeutic strategy to prevent cancer spread. This study aim to investigate the potential anti-metastatic and antiproliferative effect of total phenols extracted from EVOO against human transitional bladder carcinoma cell lines. Our results show that EVOO extract can significantly inhibit the proliferation and motility of T24 bladder cells in a dose-dependent manner. Furthermore the enzymatic activity of MMP-2 was inhibited at non-toxic EVOO extract doses in T24 cells. The qRT-PCR revealed a decrease of the MMP-2 expression, and a simultaneous increase of the TIMPs gene expression. The cell cycle analysis of two bladder cancer cell lines (T24 and 5637) after EVOO-E treatment show a marked growth arrest prior to mitosis in the G2/M phase for both the cell lines with the subsequent induction of apoptosis only in T24 cells. Finally we evaluated the ability of EVOOE to chemosensitize T24 to the cytotoxic action of common used chemoterapics. Interestingly the simultaneous treatment of Paclitaxel and EVOOE strongly increase the apoptotic cell death at every of the tested concentration if compared to the drug alone. Our results may support the epidemiological evidences which link olive oil consumption to health benefits and may represent a starting point for the development of new anticancer strategies

Defective FoxP3+ Treg cell differentiation in the gut of Type 1 Diabetic patients

Environmental factors that act at the intestinal level such as diet, drugs, and microflora have a high impact on the pathogenesis of autoimmune Type 1 Diabetes (T1D), but it is still unclear how the gut milieu affects autoimmunity outside the intestine. Here we show that peripheral FoxP3+ Treg cell differentiation, a mechanism that takes place in the gut and is crucial to maintain systemic immune tolerance, is impaired in T1D patients. These results provide the first evidence that gut mucosa alteration could predispose to autoimmune T1D by affecting systemic immune regulation

An Innovative Tool for Technical, Environmental and Economic Design of Building Energy Plants: A Case Study in Umbria

Abstract The European goals on environmental sustainability and carbon footprint reduction should be reached by the installation of Renewable Energy Sources (RES) plants. However, the massive development of RES plants has inevitably caused new relevant problems and critical situations due to their low energy density, their social acceptance and the non-programmability of some sources. A smart tool is proposed to evaluate the integration feasibility of different energy plants; this tool is also able to estimate the environmental impacts caused by the several proposed plants for building energy production in terms of land usage, carbon footprint and water consumption. A case study, a rural tower shaped building in Umbria, is analyzed to validate the innovative tool: environmental, energy and economic parameters are calculated to allow the designer to define the best energy plant configuration

architectural and energy refurbishment of the headquarter of the university of teramo

Abstract The recent seismic events, which distressed the population of the Central Italy, have caused the incompliance with national safety standards of several historical buildings. In particular, the headquarter of the University of Teramo revealed some structural damages which make necessary to operate a restoration of the buildings. It represents a rare opportunity for investigating possible applications of a cluster of technologies and approaches largely recognized as efficient and high-performing to such particular buildings in order to achieve an upgrading in terms of energy efficiency. The proposal includes the improvement of the exploitation of renewable energy sources through adequate systems, which do not modify the visual perception of the pavilions. In that regards, the required electricity could be produced by a PV roofing installed on the parking area, while a biomass boiler and an absorption chiller could allow supplying the thermal and cooling needs. Furthermore, the rain water recovery system permits to reduce the exploitation of potable water for uses which do not require a high quality, such as irrigation. On the other hand, the envelope energy efficiency could be enhanced by applying passive strategies for reducing the heat losses ( winter conditions ) and gains ( summer conditions ) through facades and roof. The results confirmed the reliability of those interventions and the consequent advantages from an economical and energy point of view

Lignin as co-product of second generation bioethanol production from ligno-cellulosic biomass

Abstract To improve the economic viability of the biofuel production from biomass, it is of increasing importance to add value to the lignin produced as a bio-residue. Moreover, to meet the goal to replace 30% of fossil fuel by biofuels by 2030, a huge amount of lignin will soon be produced. The first major step involved to add value to the unconverted lignin is its separation from other biomass constituents to give high purity lignin. In this current work, extraction of lignin from a bio-residue (containing ca. 40% lignin) from second generation bioethanol production is presented. The biomass chosen is Arundo donax L. (or giant reed), which is non-food plant, can tolerate a wide variety of ecological conditions with all types of soils, and has increasingly importance as raw material for industrial purposes as a source of fibers alternative to wood, which availability is decreasing. Slightly different extraction procedures are investigated. Methods used are simple, mild, safe, and avoid destruction of fiber content in the bio- residue, with the final aim to valorize all fractions of the bio-residue, which is an essential step to make biofuel production to be cost effective. Lignins extracted are characterized by morphological analysis, using Scanning Electron Microscopy, SEM, and in terms of thermal behavior -using thermo gravimetric analysis TGA- which is critical for determining suitability of the lignin for polymer composite preparation with improved thermomechanical performance. The method judged as the best of the three leads rapidly to extraction of lignin free from fibers and ash, with thermal behavior suitable for composite preparation

System thinking & synthesis mapping to manage product material selection process

Material selection is one of the core tasks in industrial product design practice since materials are the basis of manufactured artefacts. Over time, an increasing number of characteristics and attributes have been taken into consideration as competing and influent elements on the product’s material decision. However, even if the material selection can be efficiently supported by different methodologies, tools and platforms, still in the industrial strict routine, it is difficult to invest the right amount of time in scouting possible new material solutions to upgrade the production line. This gap between theoretical approach and practical application of new materials influences significantly the shift towards a more sustainable development. In this paper, the material selection process has been analysed as a process within a sociocultural system (enterprise). All the collected information have been mapped in cooperation with company employees to create a visual narrative of the whole work. The result, hence, is a synthesis map that provides a model for professionals to manage an aware material selection activity. The systemic view of the entire material selection process is then discussed: further improvements can be developed from the perspective of sustainable development and industrial/environmental interdependences

Domestic dog and alien North American mink as reservoirs of infectious diseases in the endangered Southern river otter

Introduced alien carnivores are host to infectious diseases that may become an important threat for native carnivore species conservation. Canine distemper virus (CDV) is thought to be transmitted among individuals by direct contact and to present viral dynamics associated with a density-dependent multi-host carnivore community. In contrast, Canine Parvovirus (CPV) is mostly transmitted by indirect contact and does not depend only on the density, but also on the social behaviour of infected as well as susceptible hosts. The objective of this study was to assess how introduced American mink (Neovison vison) can act as a bridge-host between domestic dog (Canis familiaris) and Southern river otter (Lontra provocax) in different dog and mink population density scenarios. Our data show that otters are seropositive to both CDV and PV, as well as a molecular identity to Parvovirus in dogs and minks. Furthermore, a strong positive correlation between dog population density and observed seroprevalence of CDV in dogs, minks, and otters was recorded. For Parvovirus, the observed seroprevalence in mink and otters was not correlated to a higher dog population density, but instead a relationship between dog and mink population densities and social behaviour. Our results suggest that introduced American mink and domestic dogs are reservoirs of CDV and PV, both being diseases of major importance for the conservation of native endangered carnivores in Patagonia

Influence of Egr-1 in cardiac tissue-derived mesenchymal stem cells in response to glucose variations

Mesenchymal stem cells (MSCs) represent a promising cell population for cell therapy and regenerative medicine applications. However, how variations in glucose are perceived by MSC pool is still unclear. Since, glucose metabolism is cell type and tissue dependent, this must be considered when MSCs are derived from alternative sources such as the heart. The zinc finger transcription factor Egr-1 is an important early response gene, likely to play a key role in the glucose-induced response. Our aim was to investigate how short-term changes in in vitro glucose concentrations affect multipotent cardiac tissue-derived MSCs (cMSCs) in a mouse model of Egr-1 KO (Egr-1-/-). Results showed that loss of Egr-1 does not significantly influence cMSC proliferation. In contrast, responses to glucose variations were observed in wt but not in Egr-1 -/- cMSCs by clonogenic assay. Phenotype analysis by RT-PCR showed that cMSCs Egr-1-/- lost the ability to regulate the glucose transporters GLUT-1 and GLUT-4 and, as expected, the Egr-1 target genes VEGF, TGFβ-1, and p300. Acetylated protein levels of H3 histone were impaired in Egr-1-/- compared to wt cMSCs. We propose that Egr-1 acts as immediate glucose biological sensor in cMSCs after a short period of stimuli, likely inducing epigenetic modifications. © 2014 Daniela Bastianelli et al